################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HindIII HpaII HphI KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 771 GTATAC GT^[AC][GT]AC AocI 686 CCTCAGG CC^T[ACGT]AGG AspHI 97 GTGCTC G[AT]GC[AT]^C BalI 188 TGGCCA TGG^CCA BanII 408 GAGCCC G[GA]GC[CT]^C BbsI 655 GTCTTC GAAGAC(2/6) BpuAI 655 GTCTTC GAAGAC(2/6) BsiHKAI 97 GTGCTC G[AT]GC[AT]^C BsmAI 194 GTCTC GTCTC(1/5) BsmFI 45 GGGAC GGGAC(10/14) BssKI 443 CCTGG ^CC[ACGT]GG Bst1107I 771 GTATAC GTA^TAC BstNI 443 CCTGG CC^[AT]GG BstXI 312 CCACGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 771 GTATAC GTA^TAC Bsu36I 686 CCTCAGG CC^T[ACGT]AGG CfoI 264 GCGC GCG^C DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 16 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 350 CCATGG C^C[GA][CT]GG Eco81I 686 CCTCAGG CC^T[ACGT]AGG EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRII 443 CCTGG ^CC[AT]GG HhaI 264 GCGC GCG^C HinP1I 264 GCGC G^CGC HincII 601 GTTAAC GT[CT]^[GA]AC HindII 601 GTTAAC GT[CT]^[GA]AC HpaI 601 GTTAAC GTT^AAC MluNI 188 TGGCCA TGG^CCA MscI 188 TGGCCA TGG^CCA MvaI 443 CCTGG CC^[AT]GG NcoI 350 CCATGG C^CATGG PleI 47 GACTC GAGTC(4/5) ScrFI 443 CCTGG CC^[ACGT]GG SfcI 576 CTACAG C^T[GA][CT]AG XcmI 312 CCACGTCATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 66 GATCC GGATC(4/5) 929 GGATC BmyI 97 GTGCTC G[AGT]GC[ACT]^C 408 GAGCCC BseRI 73 CTCCTC GAGGAG(10/8) 76 CTCCTC Bsp1286I 97 GTGCTC G[AGT]GC[ACT]^C 408 GAGCCC BstYI 65 AGATCC [GA]^GATC[CT] 929 GGATCT Cac8I 109 GCAAGC GC[ACGT]^[ACGT]GC 217 GCCTGC DpnII 66 GATC ^GATC 930 GATC EaeI 188 TGGCCA [CT]^GGCC[GA] 778 CGGCCA MaeII 314 ACGT A^CGT 653 ACGT MboI 66 GATC ^GATC 930 GATC MflI 65 AGATCC [GA]^GATC[CT] 929 GGATCT MseI 491 TTAA T^TAA 602 TTAA MslI 345 CATAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 762 CATGTTTGTG NdeII 66 GATC ^GATC 930 GATC NlaIV 214 GGAGCC GG[ACGT]^[ACGT]CC 446 GGACCC Sau3AI 66 GATC ^GATC 930 GATC SfaNI 823 GATGC GCATC(5/9) 290 GCATC TfiI 12 GAATC G^A[AT]TC 846 GAATC Tru9I 491 TTAA T^TAA 602 TTAA Tsp509I 493 AATT ^AATT 790 AATT XhoII 65 AGATCC [GA]^GATC[CT] 929 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AfaI AluI AvaII BbvI BfaI BsaJI BsrI BstF5I Cfr13I DdeI EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboII MnlI NlaIII RsaI Sau96I StyI TseI Tsp45I TspRI