################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstUI BstXI BstYI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AlwI 292 GGATC GGATC(4/5) ApaI 330 GGGCCC GGGCC^C AspHI 359 GTGCTC G[AT]GC[AT]^C AvaII 1262 GGACC G^G[AT]CC BanII 330 GGGCCC G[GA]GC[CT]^C BbsI 60 GTCTTC GAAGAC(2/6) BpmI 39 CTCCAG CTGGAG(16/14) BpuAI 60 GTCTTC GAAGAC(2/6) BsgI 370 GTGCAG GTGCAG(16/14) BsiHKAI 359 GTGCTC G[AT]GC[AT]^C BspMI 452 ACCTGC ACCTGC(4/8) BssKI 523 CCAGG ^CC[ACGT]GG BstNI 523 CCAGG CC^[AT]GG DraII 330 GGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 268 GACGTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 309 CCATGG C^C[GA][CT]GG EarI 1307 GAAGAG CTCTTC(1/4) EcoO109I 330 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoRII 523 CCAGG ^CC[AT]GG Ksp632I 1307 GAAGAG CTCTTC(1/4) MspA1I 68 CAGCTG C[AC]G^C[GT]G MvaI 523 CCAGG CC^[AT]GG NcoI 309 CCATGG C^CATGG NheI 76 GCTAGC G^CTAGC NlaIV 330 GGGCCC GG[ACGT]^[ACGT]CC PleI 21 GACTC GAGTC(4/5) PvuII 68 CAGCTG CAG^CTG ScrFI 523 CCAGG CC^[ACGT]GG SexAI 522 ACCAGGT A^CC[AT]GGT TaqI 222 TCGA T^CGA TthHB8I 222 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 1225 CCGC CCGC(-3/-1) 429 GCGG BfaI 77 CTAG C^TAG 146 CTAG BsaJI 81 CCAAGG C^C[ACGT][ACGT]GG 309 CCATGG BseRI 398 CTCCTC GAGGAG(10/8) 401 CTCCTC BsiYI 81 CCAAGGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1233 CCCTGTTGGGG BslI 81 CCAAGGCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1233 CCCTGTTGGGG BsmAI 19 GAGAC GTCTC(1/5) 1310 GAGAC Cac8I 49 GCCAGC GC[ACGT]^[ACGT]GC 76 GCTAGC DpnII 293 GATC ^GATC 662 GATC EcoT14I 81 CCAAGG C^C[AT][AT]GG 309 CCATGG HaeIII 48 GGCC GG^CC 331 GGCC MaeI 77 CTAG C^TAG 146 CTAG MboI 293 GATC ^GATC 662 GATC NdeII 293 GATC ^GATC 662 GATC PstI 71 CTGCAG CTGCA^G 454 CTGCAG Sau3AI 293 GATC ^GATC 662 GATC SfaNI 142 GATGC GCATC(5/9) 1218 GATGC SfcI 71 CTGCAG C^T[GA][CT]AG 454 CTGCAG StyI 81 CCAAGG C^C[AT][AT]GG 309 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AccI AcsI AluI ApoI BbvI BmyI Bsp1286I BsrI Bst1107I BstAPI BstEII BstF5I BstPI BstZ17I Cfr13I DdeI EcoO65I EcoRV Fnu4HI FokI HinfI HphI ItaI MaeII MaeIII MboII MnlI MseI MslI MwoI NlaIII Sau96I TfiI Tru9I TseI Tsp45I Tsp509I TspRI