(Circular) (Six-base) MAPSORT of: pFN21AE0766.seq Check: 8438 from: 1 to: 6019 pFN21AE0766 6019 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3829 278 Size: 53 83 186 936 2293 2468 Fragments arranged by size: 2468 2293 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2778 2850 2922 2994 3066 3138 3210 3362 Size: 841 72 72 72 72 72 72 152 Cuts at: 3362 1937 Size: 4594 Fragments arranged by size: 4594 841 152 72 72 72 72 72 72 Acc65I G'GTAC_C Cuts at: 3340 3340 Size: 6019 AclI AA'CG_TT Cuts at: 1563 4146 4519 1563 Size: 2583 373 3063 Fragments arranged by size: 3063 2583 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2348 4082 5466 5734 1632 Size: 716 1734 1384 268 1917 Fragments arranged by size: 1917 1734 1384 716 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5822 AflIII A'CryG_T Cuts at: 1203 4918 5895 1203 Size: 3715 977 1327 Fragments arranged by size: 3715 1327 977 AgeI A'CCGG_T Cuts at: 4850 4850 Size: 6019 AhdI GACnn_n'nnGTC Cuts at: 4748 4748 Size: 6019 AleI CACnn'nnGTG Cuts at: 1166 3232 1166 Size: 2066 3953 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2132 1184 Size: 948 5071 AlwNI CAG_nnn'CTG Cuts at: 3762 5334 3762 Size: 1572 4447 ApaI G_GGCC'C Cuts at: 2339 2339 Size: 6019 ApaLI G'TGCA_C Cuts at: 4076 5232 4076 Size: 1156 4863 ApoI r'AATT_y Cuts at: 1493 2737 2809 2881 2953 3025 3097 3169 3328 Size: 1244 72 72 72 72 72 72 159 Cuts at: 3328 3554 3706 5815 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1244 226 159 152 72 72 72 72 72 72 AseI AT'TA_AT Cuts at: 160 1032 4573 160 Size: 872 3541 1606 Fragments arranged by size: 3541 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6019 AvaI C'yCGr_G Cuts at: 1870 1943 3344 1870 Size: 73 1401 4545 Fragments arranged by size: 4545 1401 73 BamHI G'GATC_C Cuts at: 3349 3716 3349 Size: 367 5652 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3340 4795 618 Size: 325 217 627 1553 1455 1842 Fragments arranged by size: 1842 1553 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2339 3338 1391 Size: 948 999 4072 Fragments arranged by size: 4072 999 948 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 928 Size: 971 159 4889 Fragments arranged by size: 4889 971 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4210 4244 1903 Size: 34 2273 34 3678 Fragments arranged by size: 3678 2273 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3911 5121 3911 Size: 1210 4809 BclI T'GATC_A Cuts at: 1548 1548 Size: 6019 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4630 136 Size: 107 122 71 631 605 2958 1525 Fragments arranged by size: 2958 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6014 6014 Size: 6019 BlpI GC'TnA_GC Cuts at: 3436 3436 Size: 6019 Bme1580I G_kGCm'C Cuts at: 1790 2339 3239 4080 5236 1790 Size: 549 900 841 1156 2573 Fragments arranged by size: 2573 1156 900 841 549 BmgBI CAC'GTC Cuts at: 1318 2275 2384 1318 Size: 957 109 4953 Fragments arranged by size: 4953 957 109 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2056 3750 4708 Size: 416 53 83 399 24 639 1694 958 Cuts at: 4708 442 Size: 1753 Fragments arranged by size: 1753 1694 958 639 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2085 1056 Size: 1029 4990 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2029 4679 1239 Size: 790 2650 2579 Fragments arranged by size: 2650 2579 790 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4144 5009 5307 4144 Size: 865 298 4856 Fragments arranged by size: 4856 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4682 882 Size: 617 3183 2219 Fragments arranged by size: 3183 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3810 493 Size: 711 2606 2702 Fragments arranged by size: 2702 2606 711 BsaBI GATnn'nnATC Cuts at: 6013 6013 Size: 6019 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3826 4208 4843 275 Size: 53 83 186 936 2293 382 635 1451 Fragments arranged by size: 2293 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4452 4850 5124 5271 5682 4452 Size: 398 274 147 411 4789 Fragments arranged by size: 4789 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5794 Fragments arranged by size: 5794 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2394 2397 1186 Size: 1208 3 4808 Fragments arranged by size: 4808 1208 3 BseYI C'CCAG_C Cuts at: 2091 2332 5222 5718 2091 Size: 241 2890 496 2392 Fragments arranged by size: 2890 2392 496 241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2396 1163 Size: 573 660 4786 Fragments arranged by size: 4786 660 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3469 4230 5258 1540 Size: 275 186 1468 761 1028 2301 Fragments arranged by size: 2301 1468 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2368 4080 4165 5236 2368 Size: 1712 85 1071 3151 Fragments arranged by size: 3151 1712 1071 85 BsmI GAATG_Cn' Cuts at: 3530 3623 3530 Size: 93 5926 BsmBI CGTCTCn'nnnn_ Cuts at: 3309 3309 Size: 6019 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2339 2368 3239 3338 4080 4165 5236 Size: 399 549 29 871 99 742 85 1071 Cuts at: 5236 1391 Size: 2174 Fragments arranged by size: 2174 1071 871 742 549 399 99 85 29 BspEI T'CCGG_A Cuts at: 5682 5682 Size: 6019 BspHI T'CATG_A Cuts at: 3908 5638 3908 Size: 1730 4289 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2466 3374 844 Size: 1622 908 3489 Fragments arranged by size: 3489 1622 908 BsrBI CCG'CTC Cuts at: 1394 1823 3906 1394 Size: 429 2083 3507 Fragments arranged by size: 3507 2083 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4514 4688 65 Size: 1183 3266 174 1396 Fragments arranged by size: 3266 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4663 4850 1700 Size: 110 2853 187 2869 Fragments arranged by size: 2869 2853 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6019 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6019 BssSI C'ACGA_G Cuts at: 4079 5091 4079 Size: 1012 5007 BstAPI GCAn_nnn'nTGC Cuts at: 794 2726 2798 2870 2942 3014 3086 3158 794 Size: 1932 72 72 72 72 72 72 3655 Fragments arranged by size: 3655 1932 72 72 72 72 72 72 BstBI TT'CG_AA Cuts at: 4823 4823 Size: 6019 BstEII G'GTnAC_C Cuts at: 1184 2608 2824 2968 3112 3184 1184 Size: 1424 216 144 144 72 4019 Fragments arranged by size: 4019 1424 216 144 144 72 BstYI r'GATC_y Cuts at: 1972 3349 3716 4101 4118 5570 5656 5668 6014 Size: 1377 367 385 17 1452 86 12 346 Cuts at: 6014 1972 Size: 1977 Fragments arranged by size: 1977 1452 1377 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2779 2851 2923 2995 3067 3139 3211 2779 Size: 72 72 72 72 72 72 5587 Fragments arranged by size: 5587 72 72 72 72 72 72 BtgI C'CryG_G Cuts at: 513 1065 2314 513 Size: 552 1249 4218 Fragments arranged by size: 4218 1249 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5280 Fragments arranged by size: 5280 683 56 BtsI GCAGTG_nn' Cuts at: 2682 3239 3535 4329 4349 2682 Size: 557 296 794 20 4352 Fragments arranged by size: 4352 794 557 296 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6019 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2370 2405 529 Size: 35 1806 35 4143 Fragments arranged by size: 4143 1806 35 35 DraI TTT'AAA Cuts at: 3323 3323 Size: 6019 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2279 5026 809 Size: 507 963 2747 1802 Fragments arranged by size: 2747 1802 963 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3466 4355 5984 Size: 54 1425 50 275 18 1636 889 1629 Cuts at: 5984 8 Size: 43 Fragments arranged by size: 1636 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3466 1537 Size: 275 1654 4090 Fragments arranged by size: 4090 1654 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3307 3949 1353 Size: 1954 642 3423 Fragments arranged by size: 3423 1954 642 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2449 4606 4980 5126 387 Size: 1064 138 860 2157 374 146 1280 Fragments arranged by size: 2157 1280 1064 860 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2029 2348 4082 4679 5466 5734 1239 Size: 393 397 319 1734 597 787 268 1524 Fragments arranged by size: 1734 1524 787 597 397 393 319 268 EcoO109I rG'GnC_Cy Cuts at: 2336 2336 Size: 6019 EcoRI G'AATT_C Cuts at: 3328 3706 3328 Size: 378 5641 EcoRV GAT'ATC Cuts at: 2621 2693 2765 2837 2909 2981 3053 3125 3197 Size: 72 72 72 72 72 72 72 72 Cuts at: 3197 2621 Size: 5443 Fragments arranged by size: 5443 72 72 72 72 72 72 72 72 FspI TGC'GCA Cuts at: 4525 4525 Size: 6019 HaeII r_GCGC'y Cuts at: 1678 5166 1678 Size: 3488 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4660 4692 4734 4766 1373 Size: 32 135 32 3088 32 42 32 2626 Fragments arranged by size: 3088 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3363 3609 669 Size: 988 281 1425 246 3079 Fragments arranged by size: 3079 1425 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6019 HpaI GTT'AAC Cuts at: 3609 3609 Size: 6019 KpnI G_GTAC'C Cuts at: 3344 3344 Size: 6019 MfeI C'AATT_G Cuts at: 3618 3618 Size: 6019 MluI A'CGCG_T Cuts at: 5895 5895 Size: 6019 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5133 5317 1305 Size: 3828 184 2007 Fragments arranged by size: 3828 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4197 Fragments arranged by size: 4197 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2313 3232 3280 3977 4336 4495 518 Size: 648 1147 919 48 697 359 159 2042 Fragments arranged by size: 2042 1147 919 697 648 359 159 48 MspA1I CmG'CkG Cuts at: 1606 2075 3435 4112 5260 5505 1606 Size: 469 1360 677 1148 245 2120 Fragments arranged by size: 2120 1360 1148 677 469 245 NcoI C'CATG_G Cuts at: 513 1065 2314 513 Size: 552 1249 4218 Fragments arranged by size: 4218 1249 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6019 NheI G'CTAG_C Cuts at: 1052 2081 1052 Size: 1029 4990 NotI GC'GGCC_GC Cuts at: 3466 3466 Size: 6019 NspI r_CATG'y Cuts at: 1132 1229 3377 4922 1132 Size: 97 2148 1545 2229 Fragments arranged by size: 2229 2148 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6019 PciI A'CATG_T Cuts at: 4918 4918 Size: 6019 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6019 PmeI GTTT'AAAC Cuts at: 3323 3323 Size: 6019 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4088 4120 5601 5633 4088 Size: 32 1481 32 4474 Fragments arranged by size: 4474 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6019 PsiI TTA'TAA Cuts at: 3589 3589 Size: 6019 PspOMI G'GGCC_C Cuts at: 2335 2335 Size: 6019 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2179 2211 2179 Size: 32 5987 PstI C_TGCA'G Cuts at: 830 2080 2463 3371 830 Size: 1250 383 908 3478 Fragments arranged by size: 3478 1250 908 383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6019 PvuII CAG'CTG Cuts at: 2075 2075 Size: 6019 SalI G'TCGA_C Cuts at: 1936 3361 1936 Size: 1425 4594 SbfI CC_TGCA'GG Cuts at: 3371 3371 Size: 6019 ScaI AGT'ACT Cuts at: 4267 5979 4267 Size: 1712 4307 SexAI A'CCwGG_T Cuts at: 2527 2527 Size: 6019 SfcI C'TryA_G Cuts at: 826 1045 2076 2459 3367 4502 5183 5374 826 Size: 219 1031 383 908 1135 681 191 1471 Fragments arranged by size: 1471 1135 1031 908 681 383 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6019 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6019 SmaI CCC'GGG Cuts at: 1872 3346 1872 Size: 1474 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4123 5024 5286 820 Size: 197 926 2180 901 262 1553 Fragments arranged by size: 2180 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3810 493 Size: 3317 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6019 SphI G_CATG'C Cuts at: 1132 3377 1132 Size: 2245 3774 SspI AAT'ATT Cuts at: 5 52 3943 5 Size: 47 3891 2081 Fragments arranged by size: 3891 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6019 StyI C'CwwG_G Cuts at: 513 1065 2086 2314 3458 513 Size: 552 1021 228 1144 3074 Fragments arranged by size: 3074 1144 1021 552 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2075 2083 3284 4046 4063 4216 4401 Size: 1255 495 8 1201 762 17 153 185 Cuts at: 4401 4846 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1201 762 495 445 185 153 17 8 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4265 5977 96 Size: 274 80 33 51 1443 2288 1712 138 Fragments arranged by size: 2288 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2464 2496 5979 6011 2464 Size: 32 3483 32 2472 Fragments arranged by size: 3483 2472 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6019 XbaI T'CTAG_A Cuts at: 3355 3355 Size: 6019 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6019 XmaI C'CCGG_G Cuts at: 1870 3344 1870 Size: 1474 4545 XmnI GAAnn'nnTTC Cuts at: 4148 4148 Size: 6019 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3827 276 Size: 53 83 186 936 2293 2468 Fragments arranged by size: 2468 2293 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI