(Circular) (Six-base) MAPSORT of: pFN21AE1597.seq Check: 7930 from: 1 to: 13945 pFN21AE1597 13945 bp With 182 enzymes: SgfI * September 9, 2010 14:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3379 3565 6733 3379 Size: 186 3168 10591 Fragments arranged by size: 10591 3168 186 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2640 11755 278 Size: 53 83 186 936 1104 9115 2468 Fragments arranged by size: 9115 2468 1104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3242 3638 4304 5501 6074 10202 11288 1937 Size: 1305 396 666 1197 573 4128 1086 4594 Fragments arranged by size: 4594 4128 1305 1197 1086 666 573 396 Acc65I G'GTAC_C Cuts at: 4142 5194 11266 4142 Size: 1052 6072 6821 Fragments arranged by size: 6821 6072 1052 AclI AA'CG_TT Cuts at: 1563 9561 12072 12445 1563 Size: 7998 2511 373 3063 Fragments arranged by size: 7998 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3030 3153 3756 4815 5253 5664 5934 6336 Size: 1398 123 603 1059 438 411 270 402 Cuts at: 6336 6768 7554 7689 7977 8365 11172 12008 13392 Size: 432 786 135 288 388 2807 836 1384 Cuts at: 13392 13660 1632 Size: 268 1917 Fragments arranged by size: 2807 1917 1398 1384 1059 836 786 603 438 432 411 402 388 288 270 268 135 123 AfeI AGC'GCT Cuts at: 10236 10236 Size: 13945 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 13748 AflIII A'CryG_T Cuts at: 1203 3743 9127 9723 10503 12844 13821 1203 Size: 2540 5384 596 780 2341 977 1327 Fragments arranged by size: 5384 2540 2341 1327 977 780 596 AgeI A'CCGG_T Cuts at: 12776 12776 Size: 13945 AhdI GACnn_n'nnGTC Cuts at: 12674 12674 Size: 13945 AleI CACnn'nnGTG Cuts at: 1166 4031 5360 5483 5729 6209 6839 8600 1166 Size: 2865 1329 123 246 480 630 1761 6511 Fragments arranged by size: 6511 2865 1761 1329 630 480 246 123 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 13945 AlwNI CAG_nnn'CTG Cuts at: 3788 3994 4580 4646 8000 8624 9776 11688 13260 Size: 206 586 66 3354 624 1152 1912 1572 Cuts at: 13260 3788 Size: 4473 Fragments arranged by size: 4473 3354 1912 1572 1152 624 586 206 66 ApaLI G'TGCA_C Cuts at: 10354 12002 13158 10354 Size: 1648 1156 11141 Fragments arranged by size: 11141 1648 1156 ApoI r'AATT_y Cuts at: 1493 3148 3595 4045 4262 4396 4848 5848 5922 Size: 1655 447 450 217 134 452 1000 74 Cuts at: 5922 5983 7589 7836 8478 8810 8821 8830 8852 Size: 61 1606 247 642 332 11 9 22 Cuts at: 8852 9433 9618 9852 10102 10338 10559 10765 11254 Size: 581 185 234 250 236 221 206 489 Cuts at: 11254 11480 11632 13741 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1655 1606 1000 642 581 489 452 450 447 332 250 247 236 234 226 221 217 206 185 152 134 74 61 22 11 9 AseI AT'TA_AT Cuts at: 160 1032 7701 12499 160 Size: 872 6669 4798 1606 Fragments arranged by size: 6669 4798 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 13945 AvaI C'yCGr_G Cuts at: 1870 1943 5868 11270 1870 Size: 73 3925 5402 4545 Fragments arranged by size: 5402 4545 3925 73 AvrII C'CTAG_G Cuts at: 2482 4763 9787 2482 Size: 2281 5024 6640 Fragments arranged by size: 6640 5024 2281 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4170 4203 4464 4497 7191 7224 4170 Size: 33 261 33 2694 33 10891 Fragments arranged by size: 10891 2694 261 33 33 33 BamHI G'GATC_C Cuts at: 8784 11275 11642 8784 Size: 2491 367 11087 Fragments arranged by size: 11087 2491 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3901 4142 5194 5612 6910 Size: 325 217 627 2114 241 1052 418 1298 Cuts at: 6910 7022 7985 8432 11266 12721 618 Size: 112 963 447 2834 1455 1842 Fragments arranged by size: 2834 2114 1842 1455 1298 1052 963 627 447 418 325 241 217 112 BanII G_rGCy'C Cuts at: 1391 10646 11264 1391 Size: 9255 618 4072 Fragments arranged by size: 9255 4072 618 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2268 2640 3206 5303 6774 8622 10519 Size: 971 369 372 566 2097 1471 1848 1897 Cuts at: 10519 10796 928 Size: 277 4077 Fragments arranged by size: 4077 2097 1897 1848 1471 971 566 372 369 277 BbvCI CC'TCA_GC Cuts at: 4581 5017 5082 9408 4581 Size: 436 65 4326 9118 Fragments arranged by size: 9118 4326 436 65 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8688 8722 12136 12170 1903 Size: 34 6751 34 3414 34 3678 Fragments arranged by size: 6751 3678 3414 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2421 4198 6232 11837 13047 2421 Size: 1777 2034 5605 1210 3319 Fragments arranged by size: 5605 3319 2034 1777 1210 BclI T'GATC_A Cuts at: 1548 10344 10389 1548 Size: 8796 45 5104 Fragments arranged by size: 8796 5104 45 BfrBI ATG'CAT Cuts at: 2178 6634 9246 2178 Size: 4456 2612 6877 Fragments arranged by size: 6877 4456 2612 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3998 5603 9695 Size: 107 122 71 631 605 2326 1605 4092 Cuts at: 9695 12556 136 Size: 2861 1525 Fragments arranged by size: 4092 2861 2326 1605 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7542 10624 13940 7542 Size: 3082 3316 7547 Fragments arranged by size: 7547 3316 3082 BlpI GC'TnA_GC Cuts at: 11362 11362 Size: 13945 Bme1580I G_kGCm'C Cuts at: 1790 2490 3286 6160 10358 12006 13162 1790 Size: 700 796 2874 4198 1648 1156 2573 Fragments arranged by size: 4198 2874 2573 1648 1156 796 700 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 13945 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3288 3711 8243 Size: 416 53 83 399 24 1871 423 4532 Cuts at: 8243 8607 9995 11676 12634 442 Size: 364 1388 1681 958 1753 Fragments arranged by size: 4532 1871 1753 1681 1388 958 423 416 399 364 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 13945 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3679 6709 8344 9873 3679 Size: 3030 1635 1529 7751 Fragments arranged by size: 7751 3030 1635 1529 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2793 3423 3937 4113 7080 8314 8592 10511 Size: 1554 630 514 176 2967 1234 278 1919 Cuts at: 10511 12605 1239 Size: 2094 2579 Fragments arranged by size: 2967 2579 2094 1919 1554 1234 630 514 278 176 Bpu10I CC'TnA_GC Cuts at: 3411 4581 5017 5082 5370 8517 9022 9408 3411 Size: 1170 436 65 288 3147 505 386 7948 Fragments arranged by size: 7948 3147 1170 505 436 386 288 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2191 2480 5514 6022 11114 12070 12935 13233 2191 Size: 289 3034 508 5092 956 865 298 2903 Fragments arranged by size: 5092 3034 2903 956 865 508 298 289 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2280 3542 3650 3956 4418 5372 5615 Size: 617 781 1262 108 306 462 954 243 Cuts at: 5615 5633 5906 12608 882 Size: 18 273 6702 2219 Fragments arranged by size: 6702 2219 1262 954 781 617 462 306 273 243 108 18 BsaAI yAC'GTr Cuts at: 493 1204 3746 11736 493 Size: 711 2542 7990 2702 Fragments arranged by size: 7990 2702 2542 711 BsaBI GATnn'nnATC Cuts at: 13939 13939 Size: 13945 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2637 11120 11752 12134 Size: 53 83 186 936 1104 8483 632 382 Cuts at: 12134 12769 275 Size: 635 1451 Fragments arranged by size: 8483 1451 1104 936 635 632 382 186 83 53 BsaWI w'CCGG_w Cuts at: 10250 12378 12776 13050 13197 13608 10250 Size: 2128 398 274 147 411 10587 Fragments arranged by size: 10587 2128 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2860 2890 3022 3052 3421 Size: 30 165 30 1457 30 132 30 369 Cuts at: 3421 3451 6511 6541 7549 7579 10272 10302 1178 Size: 30 3060 30 1008 30 2693 30 4821 Fragments arranged by size: 4821 3060 2693 1457 1008 369 165 132 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3652 5099 5552 7554 7557 10255 1186 Size: 2466 1447 453 2002 3 2698 4876 Fragments arranged by size: 4876 2698 2466 2002 1447 453 3 BseYI C'CCAG_C Cuts at: 6851 6998 9451 10090 13148 13644 6851 Size: 147 2453 639 3058 496 7152 Fragments arranged by size: 7152 3058 2453 639 496 147 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2778 2883 10729 11157 1163 Size: 573 1042 105 7846 428 3951 Fragments arranged by size: 7846 3951 1042 573 428 105 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 11395 12156 13184 1540 Size: 275 186 9394 761 1028 2301 Fragments arranged by size: 9394 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3423 4880 5148 7058 8201 10358 11109 12006 12091 Size: 1457 268 1910 1143 2157 751 897 85 Cuts at: 12091 13162 3423 Size: 1071 4206 Fragments arranged by size: 4206 2157 1910 1457 1143 1071 897 751 268 85 BsmI GAATG_Cn' Cuts at: 4386 6322 9409 9747 11456 11549 4386 Size: 1936 3087 338 1709 93 6782 Fragments arranged by size: 6782 3087 1936 1709 338 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2490 3286 3423 4880 5148 6160 7058 Size: 399 700 796 137 1457 268 1012 898 Cuts at: 7058 8201 10358 10646 11109 11264 12006 12091 13162 Size: 1143 2157 288 463 155 742 85 1071 Cuts at: 13162 1391 Size: 2174 Fragments arranged by size: 2174 2157 1457 1143 1071 1012 898 796 742 700 463 399 288 268 155 137 85 BspEI T'CCGG_A Cuts at: 13608 13608 Size: 13945 BspHI T'CATG_A Cuts at: 4063 10725 11834 13564 4063 Size: 6662 1109 1730 4444 Fragments arranged by size: 6662 4444 1730 1109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3379 3565 4321 6733 11300 844 Size: 2535 186 756 2412 4567 3489 Fragments arranged by size: 4567 3489 2535 2412 756 186 BsrBI CCG'CTC Cuts at: 1394 1823 11832 1394 Size: 429 10009 3507 Fragments arranged by size: 10009 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3964 5051 6548 7818 9619 9799 12440 Size: 1183 2716 1087 1497 1270 1801 180 2641 Cuts at: 12440 12614 65 Size: 174 1396 Fragments arranged by size: 2716 2641 1801 1497 1396 1270 1183 1087 180 174 BsrFI r'CCGG_y Cuts at: 1700 1810 11052 12589 12776 1700 Size: 110 9242 1537 187 2869 Fragments arranged by size: 9242 2869 1537 187 110 BsrGI T'GTAC_A Cuts at: 96 3715 5719 6659 8301 96 Size: 3619 2004 940 1642 5740 Fragments arranged by size: 5740 3619 2004 1642 940 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 13945 BssSI C'ACGA_G Cuts at: 6151 12005 13017 6151 Size: 5854 1012 7079 Fragments arranged by size: 7079 5854 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 10877 794 Size: 10083 3862 BstBI TT'CG_AA Cuts at: 10318 12749 10318 Size: 2431 11514 BstEII G'GTnAC_C Cuts at: 1184 5095 1184 Size: 3911 10034 BstXI CCAn_nnnn'nTGG Cuts at: 2771 5191 5743 5797 6184 6994 7723 10043 10276 Size: 2420 552 54 387 810 729 2320 233 Cuts at: 10276 2771 Size: 6440 Fragments arranged by size: 6440 2420 2320 810 729 552 387 233 54 BstYI r'GATC_y Cuts at: 1972 4859 7542 7997 8784 9373 10624 11224 11275 Size: 2887 2683 455 787 589 1251 600 51 Cuts at: 11275 11642 12027 12044 13496 13582 13594 13940 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 2887 2683 1977 1452 1251 787 600 589 455 385 367 346 86 51 17 12 Bsu36I CC'TnA_GG Cuts at: 6342 8319 6342 Size: 1977 11968 BtgI C'CryG_G Cuts at: 513 1065 3081 4205 5245 5277 5304 7469 7849 Size: 552 2016 1124 1040 32 27 2165 380 Cuts at: 7849 513 Size: 6609 Fragments arranged by size: 6609 2165 2016 1124 1040 552 380 32 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 13206 Fragments arranged by size: 13206 683 56 BtsI GCAGTG_nn' Cuts at: 2678 2952 4146 4581 7026 9141 10293 10449 10997 Size: 274 1194 435 2445 2115 1152 156 548 Cuts at: 10997 11461 12255 12275 2678 Size: 464 794 20 4348 Fragments arranged by size: 4348 2445 2115 1194 1152 794 548 464 435 274 156 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 13945 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5209 5244 6739 6774 9931 9966 529 Size: 35 4645 35 1495 35 3157 35 4508 Fragments arranged by size: 4645 4508 3157 1495 35 35 35 35 DraI TTT'AAA Cuts at: 4267 9432 11249 4267 Size: 5165 1817 6963 Fragments arranged by size: 6963 5165 1817 DraIII CAC_nnn'GTG Cuts at: 8237 9512 10966 8237 Size: 1275 1454 11216 Fragments arranged by size: 11216 1454 1275 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 5833 12952 809 Size: 507 3650 867 7119 1802 Fragments arranged by size: 7119 3650 1802 867 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3304 5121 6232 Size: 54 1425 50 275 18 1474 1817 1111 Cuts at: 6232 9687 9895 11392 12281 13910 8 Size: 3455 208 1497 889 1629 43 Fragments arranged by size: 3455 1817 1629 1497 1474 1425 1111 889 275 208 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 11392 1537 Size: 275 9580 4090 Fragments arranged by size: 9580 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2110 2414 2583 6742 8769 8901 9022 9462 Size: 757 304 169 4159 2027 132 121 440 Cuts at: 9462 11041 11875 1353 Size: 1579 834 3423 Fragments arranged by size: 4159 3423 2027 1579 834 757 440 304 169 132 121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9577 10813 12532 12906 13052 387 Size: 1064 138 7988 1236 1719 374 146 1280 Fragments arranged by size: 7988 1719 1280 1236 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2793 3030 3153 3423 3756 3937 4113 Size: 393 1161 237 123 270 333 181 176 Cuts at: 4113 4815 5253 5664 5934 6336 6768 7080 7554 Size: 702 438 411 270 402 432 312 474 Cuts at: 7554 7689 7977 8314 8365 8592 10511 11172 12008 Size: 135 288 337 51 227 1919 661 836 Cuts at: 12008 12605 13392 13660 1239 Size: 597 787 268 1524 Fragments arranged by size: 1919 1524 1161 836 787 702 661 597 474 438 432 411 402 393 337 333 312 288 270 270 268 237 227 181 176 135 123 51 EcoNI CCTnn'n_nnAGG Cuts at: 3909 7830 9804 3909 Size: 3921 1974 8050 Fragments arranged by size: 8050 3921 1974 EcoO109I rG'GnC_Cy Cuts at: 3914 3991 7176 9195 10886 3914 Size: 77 3185 2019 1691 6973 Fragments arranged by size: 6973 3185 2019 1691 77 EcoRI G'AATT_C Cuts at: 3148 5848 7836 8478 8830 9852 10102 10765 11254 Size: 2700 1988 642 352 1022 250 663 489 Cuts at: 11254 11632 3148 Size: 378 5461 Fragments arranged by size: 5461 2700 1988 1022 663 642 489 378 352 250 EcoRV GAT'ATC Cuts at: 4923 6282 8148 4923 Size: 1359 1866 10720 Fragments arranged by size: 10720 1866 1359 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 7438 11116 7438 Size: 3678 10267 FspI TGC'GCA Cuts at: 12451 12451 Size: 13945 HaeII r_GCGC'y Cuts at: 1678 10238 13092 1678 Size: 8560 2854 2531 Fragments arranged by size: 8560 2854 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2031 2063 2189 2221 2942 Size: 32 135 32 459 32 126 32 721 Cuts at: 2942 2974 3218 3250 3620 3652 4355 4387 6271 Size: 32 244 32 370 32 703 32 1884 Cuts at: 6271 6303 6386 6418 6994 7026 7534 7549 7566 Size: 32 83 32 576 32 508 15 17 Cuts at: 7566 7581 8132 8164 8350 8382 9358 9390 9661 Size: 15 551 32 186 32 976 32 271 Cuts at: 9661 9693 10245 10277 10416 10448 11110 11142 12586 Size: 32 552 32 139 32 662 32 1444 Cuts at: 12586 12618 12660 12692 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1884 1444 976 721 703 662 576 552 551 508 459 370 271 244 186 139 135 126 83 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 2650 2671 3231 5673 7764 11118 Size: 988 281 712 21 560 2442 2091 3354 Cuts at: 11118 11136 11289 11535 669 Size: 18 153 246 3079 Fragments arranged by size: 3354 3079 2442 2091 988 712 560 281 246 153 21 18 HindIII A'AGCT_T Cuts at: 748 2030 11197 748 Size: 1282 9167 3496 Fragments arranged by size: 9167 3496 1282 HpaI GTT'AAC Cuts at: 11535 11535 Size: 13945 KpnI G_GTAC'C Cuts at: 4146 5198 11270 4146 Size: 1052 6072 6821 Fragments arranged by size: 6821 6072 1052 MfeI C'AATT_G Cuts at: 4452 7369 9101 11544 4452 Size: 2917 1732 2443 6853 Fragments arranged by size: 6853 2917 2443 1732 MluI A'CGCG_T Cuts at: 13821 13821 Size: 13945 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5796 6474 6971 8507 10643 11050 13059 13243 Size: 4491 678 497 1536 2136 407 2009 184 Cuts at: 13243 1305 Size: 2007 Fragments arranged by size: 4491 2136 2009 2007 1536 678 497 407 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3306 5123 6234 9897 10 Size: 54 1425 343 1474 1817 1111 3663 4058 Fragments arranged by size: 4058 3663 1817 1474 1425 1111 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2356 4031 4204 4812 5360 5483 5729 Size: 648 1190 1675 173 608 548 123 246 Cuts at: 5729 6209 6839 6992 7811 8467 8600 9756 10694 Size: 480 630 153 819 656 133 1156 938 Cuts at: 10694 10952 11903 12262 12421 518 Size: 258 951 359 159 2042 Fragments arranged by size: 2042 1675 1190 1156 951 938 819 656 648 630 608 548 480 359 258 246 173 159 153 133 123 MspA1I CmG'CkG Cuts at: 1606 3302 3329 3434 3572 3848 4184 4856 5006 Size: 1696 27 105 138 276 336 672 150 Cuts at: 5006 6416 7667 10439 11361 12038 13186 13431 1606 Size: 1410 1251 2772 922 677 1148 245 2120 Fragments arranged by size: 2772 2120 1696 1410 1251 1148 922 677 672 336 276 245 150 138 105 27 NcoI C'CATG_G Cuts at: 513 1065 3081 4205 5245 5304 7469 7849 513 Size: 552 2016 1124 1040 59 2165 380 6609 Fragments arranged by size: 6609 2165 2016 1124 1040 552 380 59 NdeI CA'TA_TG Cuts at: 387 2128 5043 6229 387 Size: 1741 2915 1186 8103 Fragments arranged by size: 8103 2915 1741 1186 NheI G'CTAG_C Cuts at: 1052 1052 Size: 13945 NotI GC'GGCC_GC Cuts at: 11392 11392 Size: 13945 NsiI A_TGCA'T Cuts at: 2180 6636 9248 2180 Size: 4456 2612 6877 Fragments arranged by size: 6877 4456 2612 NspI r_CATG'y Cuts at: 1132 1229 2178 10507 11303 12848 1132 Size: 97 949 8329 796 1545 2229 Fragments arranged by size: 8329 2229 1545 949 796 97 PasI CC'CwG_GG Cuts at: 1348 4900 5797 9457 1348 Size: 3552 897 3660 5836 Fragments arranged by size: 5836 3660 3552 897 PciI A'CATG_T Cuts at: 10503 12844 10503 Size: 2341 11604 PflMI CCAn_nnn'nTGG Cuts at: 1108 2578 5063 1108 Size: 1470 2485 9990 Fragments arranged by size: 9990 2485 1470 PfoI T'CCnGG_A Cuts at: 2577 6917 7649 8569 2577 Size: 4340 732 920 7953 Fragments arranged by size: 7953 4340 920 732 PmeI GTTT'AAAC Cuts at: 11249 11249 Size: 13945 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 12014 12046 13527 13559 12014 Size: 32 1481 32 12400 Fragments arranged by size: 12400 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3914 7176 9195 10886 3914 Size: 3262 2019 1691 6973 Fragments arranged by size: 6973 3262 2019 1691 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 13945 PsiI TTA'TAA Cuts at: 8804 10241 11515 8804 Size: 1437 1274 11234 Fragments arranged by size: 11234 1437 1274 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6963 6995 8206 8238 6963 Size: 32 1211 32 12670 Fragments arranged by size: 12670 1211 32 32 PstI C_TGCA'G Cuts at: 830 10020 10444 11297 830 Size: 9190 424 853 3478 Fragments arranged by size: 9190 3478 853 424 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 13945 PvuII CAG'CTG Cuts at: 3302 3329 3434 3572 3848 4184 4856 5006 6416 Size: 27 105 138 276 336 672 150 1410 Cuts at: 6416 7667 10439 3302 Size: 1251 2772 6808 Fragments arranged by size: 6808 2772 1410 1251 672 336 276 150 138 105 27 SalI G'TCGA_C Cuts at: 1936 11287 1936 Size: 9351 4594 SanDI GG'GwC_CC Cuts at: 10886 10886 Size: 13945 SapI GCTCTTCn'nnn_ Cuts at: 8769 9022 8769 Size: 253 13692 SbfI CC_TGCA'GG Cuts at: 11297 11297 Size: 13945 ScaI AGT'ACT Cuts at: 7531 12193 13905 7531 Size: 4662 1712 7571 Fragments arranged by size: 7571 4662 1712 SexAI A'CCwGG_T Cuts at: 10377 10377 Size: 13945 SfcI C'TryA_G Cuts at: 826 1045 3324 3780 3843 4164 4566 4950 5001 Size: 219 2279 456 63 321 402 384 51 Cuts at: 5001 6462 6894 8103 10016 10203 10440 11293 12428 Size: 1461 432 1209 1913 187 237 853 1135 Cuts at: 12428 13109 13300 826 Size: 681 191 1471 Fragments arranged by size: 2279 1913 1471 1461 1209 1135 853 681 456 432 402 384 321 237 219 191 187 63 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 13945 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 13945 SmaI CCC'GGG Cuts at: 1872 5870 11272 1872 Size: 3998 5402 4545 Fragments arranged by size: 5402 4545 3998 SmlI C'TyrA_G Cuts at: 820 1017 1943 2206 2495 5529 6037 11129 12049 Size: 197 926 263 289 3034 508 5092 920 Cuts at: 12049 12950 13212 820 Size: 901 262 1553 Fragments arranged by size: 5092 3034 1553 926 920 901 508 289 263 262 197 SnaBI TAC'GTA Cuts at: 493 11736 493 Size: 11243 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 13945 SphI G_CATG'C Cuts at: 1132 2178 11303 1132 Size: 1046 9125 3774 Fragments arranged by size: 9125 3774 1046 SspI AAT'ATT Cuts at: 5 52 4088 7297 9385 11869 5 Size: 47 4036 3209 2088 2484 2081 Fragments arranged by size: 4036 3209 2484 2088 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 13945 StyI C'CwwG_G Cuts at: 513 1065 2272 2482 3081 3344 3563 3897 4205 Size: 552 1207 210 599 263 219 334 308 Cuts at: 4205 4337 4763 5245 5304 7469 7849 8780 9787 Size: 132 426 482 59 2165 380 931 1007 Cuts at: 9787 11384 513 Size: 1597 3074 Fragments arranged by size: 3074 2165 1597 1207 1007 931 599 552 482 426 380 334 308 263 219 210 132 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3845 4892 8032 8172 8225 10082 10184 Size: 1255 2265 1047 3140 140 53 1857 102 Cuts at: 10184 11004 11972 11989 12142 12327 12772 325 Size: 820 968 17 153 185 445 1498 Fragments arranged by size: 3140 2265 1857 1498 1255 1047 968 820 445 185 153 140 102 53 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2550 2723 3715 Size: 274 80 33 51 1443 573 173 992 Cuts at: 3715 5719 6659 7529 8301 12191 13903 96 Size: 2004 940 870 772 3890 1712 138 Fragments arranged by size: 3890 2004 1712 1443 992 940 870 772 573 274 173 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3421 3453 3697 3729 6372 6404 10272 10304 13905 Size: 32 244 32 2643 32 3868 32 3601 Cuts at: 13905 13937 3421 Size: 32 3429 Fragments arranged by size: 3868 3601 3429 2643 244 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 8362 8395 1316 Size: 7046 33 6866 Fragments arranged by size: 7046 6866 33 XbaI T'CTAG_A Cuts at: 11281 11281 Size: 13945 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6991 7579 9728 10204 6991 Size: 588 2149 476 10732 Fragments arranged by size: 10732 2149 588 476 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 13945 XmaI C'CCGG_G Cuts at: 1870 5868 11270 1870 Size: 3998 5402 4545 Fragments arranged by size: 5402 4545 3998 XmnI GAAnn'nnTTC Cuts at: 3096 10901 12074 3096 Size: 7805 1173 4967 Fragments arranged by size: 7805 4967 1173 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2638 11753 276 Size: 53 83 186 936 1104 9115 2468 Fragments arranged by size: 9115 2468 1104 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BbeI BsiWI BsmBI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SfiI SfoI SrfI SwaI