(Circular) (Six-base) MAPSORT of: pFN21AE2360.seq Check: 2103 from: 1 to: 10924 pFN21AE2360 10924 bp With 182 enzymes: SgfI * September 13, 2011 14:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5638 5638 Size: 10924 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8734 278 Size: 53 83 186 936 7198 2468 Fragments arranged by size: 7198 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2261 3255 5356 6548 8267 1937 Size: 324 994 2101 1192 1719 4594 Fragments arranged by size: 4594 2101 1719 1192 994 324 Acc65I G'GTAC_C Cuts at: 4282 8245 4282 Size: 3963 6961 AclI AA'CG_TT Cuts at: 1563 3100 7135 9051 9424 1563 Size: 1537 4035 1916 373 3063 Fragments arranged by size: 4035 3063 1916 1537 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5117 5369 6089 6183 6231 6372 6393 7279 Size: 3485 252 720 94 48 141 21 886 Cuts at: 7279 8238 8987 10371 10639 1632 Size: 959 749 1384 268 1917 Fragments arranged by size: 3485 1917 1384 959 886 749 720 268 252 141 94 48 21 AfeI AGC'GCT Cuts at: 5102 5102 Size: 10924 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10727 AflIII A'CryG_T Cuts at: 1203 6367 6435 7959 8155 9823 10800 1203 Size: 5164 68 1524 196 1668 977 1327 Fragments arranged by size: 5164 1668 1524 1327 977 196 68 AgeI A'CCGG_T Cuts at: 9755 9755 Size: 10924 AhdI GACnn_n'nnGTC Cuts at: 9653 9653 Size: 10924 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 10924 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2270 7887 1184 Size: 1086 5617 4221 Fragments arranged by size: 5617 4221 1086 AlwNI CAG_nnn'CTG Cuts at: 4076 4327 6209 6917 8667 10239 4076 Size: 251 1882 708 1750 1572 4761 Fragments arranged by size: 4761 1882 1750 1572 708 251 ApaI G_GGCC'C Cuts at: 2293 2293 Size: 10924 ApaLI G'TGCA_C Cuts at: 3530 4487 8981 10137 3530 Size: 957 4494 1156 4317 Fragments arranged by size: 4494 4317 1156 957 ApoI r'AATT_y Cuts at: 1493 2021 2641 3396 3426 3499 4010 4493 5207 Size: 528 620 755 30 73 511 483 714 Cuts at: 5207 7248 7386 7970 8059 8233 8459 8611 10720 Size: 2041 138 584 89 174 226 152 2109 Cuts at: 10720 1493 Size: 1697 Fragments arranged by size: 2109 2041 1697 755 714 620 584 528 511 483 226 174 152 138 89 73 30 AseI AT'TA_AT Cuts at: 160 1032 6299 7734 9478 160 Size: 872 5267 1435 1744 1606 Fragments arranged by size: 5267 1744 1606 1435 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10924 AvaI C'yCGr_G Cuts at: 1870 1943 4959 4976 8249 1870 Size: 73 3016 17 3273 4545 Fragments arranged by size: 4545 3273 3016 73 17 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5486 5519 6503 6536 5486 Size: 33 984 33 9874 Fragments arranged by size: 9874 984 33 33 BamHI G'GATC_C Cuts at: 8254 8621 8254 Size: 367 10557 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4282 8245 9700 618 Size: 325 217 627 2495 3963 1455 1842 Fragments arranged by size: 3963 2495 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2064 2096 2130 2293 3189 3296 3335 4551 Size: 673 32 34 163 896 107 39 1216 Cuts at: 4551 5934 6855 8243 1391 Size: 1383 921 1388 4072 Fragments arranged by size: 4072 1388 1383 1216 921 896 673 163 107 39 34 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2454 3010 3696 7562 8124 928 Size: 971 555 556 686 3866 562 3728 Fragments arranged by size: 3866 3728 971 686 562 556 555 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 9115 9149 1903 Size: 34 7178 34 3678 Fragments arranged by size: 7178 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5818 7938 8816 10026 5818 Size: 2120 878 1210 6716 Fragments arranged by size: 6716 2120 1210 878 BclI T'GATC_A Cuts at: 1548 1548 Size: 10924 BfrBI ATG'CAT Cuts at: 2938 5450 7264 8128 2938 Size: 2512 1814 864 5734 Fragments arranged by size: 5734 2512 1814 864 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3330 9535 136 Size: 107 122 71 631 605 1658 6205 1525 Fragments arranged by size: 6205 1658 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2191 4477 10919 2191 Size: 2286 6442 2196 Fragments arranged by size: 6442 2286 2196 BlpI GC'TnA_GC Cuts at: 2036 2058 6823 8341 2036 Size: 22 4765 1518 4619 Fragments arranged by size: 4765 4619 1518 22 Bme1580I G_kGCm'C Cuts at: 1790 2293 2702 3534 3825 4045 4491 8985 10141 Size: 503 409 832 291 220 446 4494 1156 Cuts at: 10141 1790 Size: 2573 Fragments arranged by size: 4494 2573 1156 832 503 446 409 291 220 BmgBI CAC'GTC Cuts at: 1318 7816 1318 Size: 6498 4426 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5806 8655 9613 Size: 416 53 83 399 24 4389 2849 958 Cuts at: 9613 442 Size: 1753 Fragments arranged by size: 4389 2849 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5091 7923 1056 Size: 4035 2832 4057 Fragments arranged by size: 4057 4035 2832 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2386 3666 4803 5741 2386 Size: 1280 1137 938 7569 Fragments arranged by size: 7569 1280 1137 938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4248 6797 7112 9584 1239 Size: 3009 2549 315 2472 2579 Fragments arranged by size: 3009 2579 2549 2472 315 Bpu10I CC'TnA_GC Cuts at: 6854 6854 Size: 10924 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2714 3603 3683 4458 7197 7414 9049 9914 10212 Size: 889 80 775 2739 217 1635 865 298 Cuts at: 10212 2714 Size: 3426 Fragments arranged by size: 3426 2739 1635 889 865 775 298 217 80 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2401 9587 882 Size: 617 902 7186 2219 Fragments arranged by size: 7186 2219 902 617 BsaAI yAC'GTr Cuts at: 493 1204 2344 7696 8715 493 Size: 711 1140 5352 1019 2702 Fragments arranged by size: 5352 2702 1140 1019 711 BsaBI GATnn'nnATC Cuts at: 4734 5301 6321 10918 4734 Size: 567 1020 4597 4740 Fragments arranged by size: 4740 4597 1020 567 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2676 8731 9113 9748 Size: 53 83 186 936 1143 6055 382 635 Cuts at: 9748 275 Size: 1451 Fragments arranged by size: 6055 1451 1143 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2941 9357 9755 10029 10176 10587 2941 Size: 6416 398 274 147 411 3278 Fragments arranged by size: 6416 3278 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2777 2807 1178 Size: 30 165 30 1374 30 9295 Fragments arranged by size: 9295 1374 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3009 3642 4776 7795 1186 Size: 1823 633 1134 3019 4315 Fragments arranged by size: 4315 3019 1823 1134 633 BseYI C'CCAG_C Cuts at: 4794 7052 10127 10623 4794 Size: 2258 3075 496 5095 Fragments arranged by size: 5095 3075 2258 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 3501 4663 5595 6457 6495 7883 Size: 573 540 1225 1162 932 862 38 1388 Cuts at: 7883 1163 Size: 4204 Fragments arranged by size: 4204 1388 1225 1162 932 862 573 540 38 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8374 9135 10163 1540 Size: 275 186 6373 761 1028 2301 Fragments arranged by size: 6373 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 3534 4491 4551 4786 5654 5934 6855 8985 Size: 1470 957 60 235 868 280 921 2130 Cuts at: 8985 9070 10141 2064 Size: 85 1071 2847 Fragments arranged by size: 2847 2130 1470 1071 957 921 868 280 235 85 60 BsmI GAATG_Cn' Cuts at: 3582 8128 8435 8528 3582 Size: 4546 307 93 5978 Fragments arranged by size: 5978 4546 307 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2566 2849 2566 Size: 283 10641 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2096 2130 2293 2702 3189 3296 Size: 399 274 32 34 163 409 487 107 Cuts at: 3296 3335 3534 3825 4045 4491 4551 4786 5654 Size: 39 199 291 220 446 60 235 868 Cuts at: 5654 5934 6855 8243 8985 9070 10141 1391 Size: 280 921 1388 742 85 1071 2174 Fragments arranged by size: 2174 1388 1071 921 868 742 487 446 409 399 291 280 274 235 220 199 163 107 85 60 39 34 32 BspEI T'CCGG_A Cuts at: 2941 10587 2941 Size: 7646 3278 BspHI T'CATG_A Cuts at: 4593 8813 10543 4593 Size: 4220 1730 4974 Fragments arranged by size: 4974 4220 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4075 5540 5638 7855 8279 844 Size: 3231 1465 98 2217 424 3489 Fragments arranged by size: 3489 3231 2217 1465 424 98 BsrBI CCG'CTC Cuts at: 1394 1823 8011 8811 1394 Size: 429 6188 800 3507 Fragments arranged by size: 6188 3507 800 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3993 4397 4504 5376 7044 7989 9419 Size: 1183 2745 404 107 872 1668 945 1430 Cuts at: 9419 9593 65 Size: 174 1396 Fragments arranged by size: 2745 1668 1430 1396 1183 945 872 404 174 107 BsrFI r'CCGG_y Cuts at: 1700 1810 9568 9755 1700 Size: 110 7758 187 2869 Fragments arranged by size: 7758 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10924 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10924 BssSI C'ACGA_G Cuts at: 8984 9996 8984 Size: 1012 9912 BstAPI GCAn_nnn'nTGC Cuts at: 794 4327 6061 794 Size: 3533 1734 5657 Fragments arranged by size: 5657 3533 1734 BstBI TT'CG_AA Cuts at: 2383 6073 9728 2383 Size: 3690 3655 3579 Fragments arranged by size: 3690 3655 3579 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10924 BstYI r'GATC_y Cuts at: 1972 2191 4477 4584 5176 8254 8621 9006 9023 Size: 219 2286 107 592 3078 367 385 17 Cuts at: 9023 10475 10561 10573 10919 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 3078 2286 1977 1452 592 385 367 346 219 107 86 17 12 BstZ17I GTA'TAC Cuts at: 6549 6549 Size: 10924 Bsu36I CC'TnA_GG Cuts at: 4788 5181 5925 4788 Size: 393 744 9787 Fragments arranged by size: 9787 744 393 BtgI C'CryG_G Cuts at: 513 1065 2004 2097 4578 4683 513 Size: 552 939 93 2481 105 6754 Fragments arranged by size: 6754 2481 939 552 105 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6935 487 Size: 683 56 5709 4476 Fragments arranged by size: 5709 4476 683 56 BtsI GCAGTG_nn' Cuts at: 5003 6762 8440 9234 9254 5003 Size: 1759 1678 794 20 6673 Fragments arranged by size: 6673 1759 1678 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10924 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3163 3198 529 Size: 35 2599 35 8255 Fragments arranged by size: 8255 2599 35 35 DraI TTT'AAA Cuts at: 4563 6276 6465 7740 8228 4563 Size: 1713 189 1275 488 7259 Fragments arranged by size: 7259 1713 1275 488 189 DraIII CAC_nnn'GTG Cuts at: 3479 6255 3479 Size: 2776 8148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9931 809 Size: 507 8615 1802 Fragments arranged by size: 8615 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3309 4920 Size: 54 1425 50 275 18 177 1302 1611 Cuts at: 4920 6286 6759 6879 7312 8371 9260 10889 8 Size: 1366 473 120 433 1059 889 1629 43 Fragments arranged by size: 1629 1611 1425 1366 1302 1059 889 473 433 275 177 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8371 1537 Size: 275 6559 4090 Fragments arranged by size: 6559 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2956 3301 3340 3867 4634 4932 5388 6075 Size: 1603 345 39 527 767 298 456 687 Cuts at: 6075 6421 7074 7233 7697 8854 1353 Size: 346 653 159 464 1157 3423 Fragments arranged by size: 3423 1603 1157 767 687 653 527 464 456 346 345 298 159 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4327 9511 9885 10031 387 Size: 1064 138 2738 5184 374 146 1280 Fragments arranged by size: 5184 2738 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2062 4549 5932 6853 2062 Size: 2487 1383 921 6133 Fragments arranged by size: 6133 2487 1383 921 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4248 5117 5369 6089 6183 6231 6372 Size: 393 2616 869 252 720 94 48 141 Cuts at: 6372 6393 6797 7112 7279 8238 8987 9584 10371 Size: 21 404 315 167 959 749 597 787 Cuts at: 10371 10639 1239 Size: 268 1524 Fragments arranged by size: 2616 1524 959 869 787 749 720 597 404 393 315 268 252 167 141 94 48 21 EcoNI CCTnn'n_nnAGG Cuts at: 6568 6568 Size: 10924 EcoO109I rG'GnC_Cy Cuts at: 2289 7603 2289 Size: 5314 5610 EcoRI G'AATT_C Cuts at: 4010 8233 8611 4010 Size: 4223 378 6323 Fragments arranged by size: 6323 4223 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3644 4777 5359 7543 3644 Size: 1133 582 2184 7025 Fragments arranged by size: 7025 2184 1133 582 FspI TGC'GCA Cuts at: 2018 9430 2018 Size: 7412 3512 HaeII r_GCGC'y Cuts at: 1678 5104 10071 1678 Size: 3426 4967 2531 Fragments arranged by size: 4967 3426 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2224 2256 2410 2442 2453 Size: 32 135 32 652 32 154 32 11 Cuts at: 2453 2456 2485 2488 2923 2955 2997 3029 4364 Size: 3 29 3 435 32 42 32 1335 Cuts at: 4364 4396 4844 4876 4972 5004 5141 5173 6035 Size: 32 448 32 96 32 137 32 862 Cuts at: 6035 6067 6224 6256 6378 6410 6562 6594 9565 Size: 32 157 32 122 32 152 32 2971 Cuts at: 9565 9597 9639 9671 1373 Size: 32 42 32 2626 Fragments arranged by size: 2971 2626 1335 862 652 448 435 157 154 152 137 135 122 96 42 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 29 11 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2243 8268 8514 669 Size: 988 281 305 6025 246 3079 Fragments arranged by size: 6025 3079 988 305 281 246 HindIII A'AGCT_T Cuts at: 748 3677 4952 7796 748 Size: 2929 1275 2844 3876 Fragments arranged by size: 3876 2929 2844 1275 HpaI GTT'AAC Cuts at: 8514 8514 Size: 10924 KpnI G_GTAC'C Cuts at: 4286 8249 4286 Size: 3963 6961 MfeI C'AATT_G Cuts at: 3226 5498 7746 8523 3226 Size: 2272 2248 777 5627 Fragments arranged by size: 5627 2272 2248 777 MluI A'CGCG_T Cuts at: 10800 10800 Size: 10924 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3024 4789 4914 5497 7858 10038 10222 1305 Size: 1719 1765 125 583 2361 2180 184 2007 Fragments arranged by size: 2361 2180 2007 1765 1719 583 184 125 MscI TGG'CCA Cuts at: 10 64 1489 1832 3311 4922 6288 6761 6881 Size: 54 1425 343 1479 1611 1366 473 120 Cuts at: 6881 7314 10 Size: 433 3620 Fragments arranged by size: 3620 1611 1479 1425 1366 473 433 343 120 54 MslI CAynn'nnrTG Cuts at: 518 1166 3933 4646 4659 7074 8882 9241 9400 Size: 648 2767 713 13 2415 1808 359 159 Cuts at: 9400 518 Size: 2042 Fragments arranged by size: 2767 2415 2042 1808 713 648 359 159 13 MspA1I CmG'CkG Cuts at: 1606 4327 4513 4798 5287 8340 9017 10165 10410 Size: 2721 186 285 489 3053 677 1148 245 Cuts at: 10410 1606 Size: 2120 Fragments arranged by size: 3053 2721 2120 1148 677 489 285 245 186 NcoI C'CATG_G Cuts at: 513 1065 2004 4578 4683 513 Size: 552 939 2574 105 6754 Fragments arranged by size: 6754 2574 939 552 105 NdeI CA'TA_TG Cuts at: 387 3817 7888 387 Size: 3430 4071 3423 Fragments arranged by size: 4071 3430 3423 NheI G'CTAG_C Cuts at: 1052 5087 7919 1052 Size: 4035 2832 4057 Fragments arranged by size: 4057 4035 2832 NotI GC'GGCC_GC Cuts at: 8371 8371 Size: 10924 NsiI A_TGCA'T Cuts at: 2940 5452 7266 8130 2940 Size: 2512 1814 864 5734 Fragments arranged by size: 5734 2512 1814 864 NspI r_CATG'y Cuts at: 1132 1229 2938 4487 4577 5450 6371 6439 7963 Size: 97 1709 1549 90 873 921 68 1524 Cuts at: 7963 7986 8159 8282 9827 1132 Size: 23 173 123 1545 2229 Fragments arranged by size: 2229 1709 1549 1545 1524 921 873 173 123 97 90 68 23 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10924 PciI A'CATG_T Cuts at: 6367 6435 7959 8155 9823 6367 Size: 68 1524 196 1668 7468 Fragments arranged by size: 7468 1668 1524 196 68 PflMI CCAn_nnn'nTGG Cuts at: 1108 2758 1108 Size: 1650 9274 PfoI T'CCnGG_A Cuts at: 4014 4014 Size: 10924 PmeI GTTT'AAAC Cuts at: 8228 8228 Size: 10924 PmlI CAC'GTG Cuts at: 2344 2344 Size: 10924 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7353 7385 8993 9025 10506 10538 7353 Size: 32 1608 32 1481 32 7739 Fragments arranged by size: 7739 1608 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10924 PsiI TTA'TAA Cuts at: 5426 8494 5426 Size: 3068 7856 PspOMI G'GGCC_C Cuts at: 2289 2289 Size: 10924 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3765 3797 3765 Size: 32 10892 PstI C_TGCA'G Cuts at: 830 3744 4072 4852 5286 7786 8276 830 Size: 2914 328 780 434 2500 490 3478 Fragments arranged by size: 3478 2914 2500 780 490 434 328 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10924 PvuII CAG'CTG Cuts at: 4327 4513 4798 4327 Size: 186 285 10453 Fragments arranged by size: 10453 285 186 SacI G_AGCT'C Cuts at: 2064 4551 5934 6855 2064 Size: 2487 1383 921 6133 Fragments arranged by size: 6133 2487 1383 921 SalI G'TCGA_C Cuts at: 1936 8266 1936 Size: 6330 4594 SapI GCTCTTCn'nnn_ Cuts at: 3301 3340 5388 6075 3301 Size: 39 2048 687 8150 Fragments arranged by size: 8150 2048 687 39 SbfI CC_TGCA'GG Cuts at: 4072 8276 4072 Size: 4204 6720 ScaI AGT'ACT Cuts at: 4883 6228 9172 10884 4883 Size: 1345 2944 1712 4923 Fragments arranged by size: 4923 2944 1712 1345 SfcI C'TryA_G Cuts at: 826 1045 2326 3458 3740 4068 4848 5077 5282 Size: 219 1281 1132 282 328 780 229 205 Cuts at: 5282 6581 7782 8272 9407 10088 10279 826 Size: 1299 1201 490 1135 681 191 1471 Fragments arranged by size: 1471 1299 1281 1201 1135 1132 780 681 490 328 282 229 219 205 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10924 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10924 SmaI CCC'GGG Cuts at: 1872 8251 1872 Size: 6379 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2729 3618 3698 4473 7212 7393 Size: 197 926 786 889 80 775 2739 181 Cuts at: 7393 9028 9929 10191 820 Size: 1635 901 262 1553 Fragments arranged by size: 2739 1635 1553 926 901 889 786 775 262 197 181 80 SnaBI TAC'GTA Cuts at: 493 8715 493 Size: 8222 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10924 SphI G_CATG'C Cuts at: 1132 2938 4577 7986 8282 1132 Size: 1806 1639 3409 296 3774 Fragments arranged by size: 3774 3409 1806 1639 296 SspI AAT'ATT Cuts at: 5 52 3856 3899 4559 4836 8848 5 Size: 47 3804 43 660 277 4012 2081 Fragments arranged by size: 4012 3804 2081 660 277 47 43 StuI AGG'CCT Cuts at: 1517 1517 Size: 10924 StyI C'CwwG_G Cuts at: 513 1065 2004 2893 3562 3981 4578 4683 8363 Size: 552 939 889 669 419 597 105 3680 Cuts at: 8363 513 Size: 3074 Fragments arranged by size: 3680 3074 939 889 669 597 552 419 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3187 6060 7574 8205 8951 8968 9121 Size: 1255 1607 2873 1514 631 746 17 153 Cuts at: 9121 9306 9751 325 Size: 185 445 1498 Fragments arranged by size: 2873 1607 1514 1498 1255 746 631 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4881 6226 7347 Size: 274 80 33 51 1443 2904 1345 1121 Cuts at: 7347 9170 10882 96 Size: 1823 1712 138 Fragments arranged by size: 2904 1823 1712 1443 1345 1121 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 10884 10916 10884 Size: 32 10892 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10924 XbaI T'CTAG_A Cuts at: 8260 8260 Size: 10924 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4930 4930 Size: 10924 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10924 XmaI C'CCGG_G Cuts at: 1870 8249 1870 Size: 6379 4545 XmnI GAAnn'nnTTC Cuts at: 2418 2564 3429 3693 3780 9053 2418 Size: 146 865 264 87 5273 4289 Fragments arranged by size: 5273 4289 865 264 146 87 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8732 276 Size: 53 83 186 936 7198 2468 Fragments arranged by size: 7198 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BbvCI BsiWI BstXI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI