################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI AhdI AlwI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII BalI BamHI BanI BbeI BbrPI BcgI BclI BglI BglII BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CfoI Cfr10I CpoI DpnII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MboI MflI MluI MluNI MroI MscI MspA1I MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AciI 1314 CCGC CCGC(-3/-1) AflII 1348 CTTAAG C^TTAAG Alw44I 110 GTGCAC G^TGCAC AlwNI 1945 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 110 GTGCAC G^TGCAC AvaI 1855 CTCGAG C^[CT]CG[GA]G AvrII 504 CCTAGG C^CTAGG BbsI 285 GTCTTC GAAGAC(2/6) BbvCI 907 GCTGAGG CCTCAGC(-5/-2) BbvI 1539 GCAGC GCAGC(8/12) BciVI 1627 GTATCC GTATCC(6/5) BcnI 1027 CCCGG CC^[GC]GG BfrI 1348 CTTAAG C^TTAAG BlnI 504 CCTAGG C^CTAGG BlpI 860 GCTCAGC GC^T[ACGT]AGC Bpu1102I 860 GCTCAGC GC^T[ACGT]AGC BpuAI 285 GTCTTC GAAGAC(2/6) BsaBI 1987 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 752 GAGACC GGTCTC(1/5) BsgI 1562 CTGCAC GTGCAG(16/14) BsmFI 1361 GGGAC GGGAC(10/14) BsoBI 1855 CTCGAG C^[CT]CG[GA]G Bsp1407I 1157 TGTACA T^GTACA BspDI 942 ATCGAT AT^CGAT BsrGI 1157 TGTACA T^GTACA BstAPI 1944 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 912 GGATG GGATG(2/0) BstXI 884 CCATTGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 860 GCTCAGC GC^T[ACGT]AGC ClaI 942 ATCGAT AT^CGAT DraI 1879 TTTAAA TTT^AAA DraII 1518 AGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 235 GACATCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1123 CCACGG C^C[GA][CT]GG EcoO109I 1518 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 827 GAATTC G^AATTC Fnu4HI 1539 GCAGC GC^[ACGT]GC FokI 912 GGATG GGATG(9/13) HapII 1028 CCGG C^CGG HincII 1432 GTTGAC GT[CT]^[GA]AC HindII 1432 GTTGAC GT[CT]^[GA]AC HpaII 1028 CCGG C^CGG ItaI 1539 GCAGC GC^[ACGT]GC MamI 1987 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1216 CAATTG C^AATTG MslI 885 CATTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1028 CCGG C^CGG MunI 1216 CAATTG C^AATTG NciI 1027 CCCGG CC^[GC]GG PaeR7I 1855 CTCGAG C^TCGAG PleI 1546 GAGTC GAGTC(4/5) SacI 1852 GAGCTC GAGCT^C SspBI 1157 TGTACA T^GTACA TseI 1539 GCAGC G^C[AT]GC XbaI 1291 TCTAGA T^CTAGA XhoI 1855 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BseRI 1193 CTCCTC GAGGAG(10/8) 1514 GAGGAG EcoNI 1507 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1591 CCTGAAGTAGG EcoT14I 504 CCTAGG C^C[AT][AT]GG 1273 CCTTGG HindIII 608 AAGCTT A^AGCTT 1902 AAGCTT HphI 508 GGTGA GGTGA(8/7) 1062 GGTGA MaeII 9 ACGT A^CGT 148 ACGT NlaIV 214 GGAACC GG[ACGT]^[ACGT]CC 2007 GGAGCC SapI 931 GAAGAGC GCTCTTC(1/4) 1660 GAAGAGC SspI 259 AATATT AAT^ATT 1066 AATATT StyI 504 CCTAGG C^C[AT][AT]GG 1273 CCTTGG Tsp45I 1328 GTGAC ^GT[GC]AC 1769 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AcsI AfaI AluI ApoI Asp700I AspHI AvaII BanII BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstNI Cac8I Cfr13I DdeI EarI EcoRII HaeIII HinfI Ksp632I MaeI MaeIII MboII MnlI MseI MvaI MwoI NlaIII RsaI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I XmnI