(Circular) (Six-base) MAPSORT of: pFN21AB6265.seq Check: 4044 from: 1 to: 6775 pFN21AB6265 6775 bp With 182 enzymes: SgfI * March 28, 2012 13:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4585 278 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4118 1937 Size: 2181 4594 Acc65I G'GTAC_C Cuts at: 4096 4096 Size: 6775 AclI AA'CG_TT Cuts at: 1563 4902 5275 1563 Size: 3339 373 3063 Fragments arranged by size: 3339 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2122 2589 3618 3669 3813 3875 4838 6222 Size: 490 467 1029 51 144 62 963 1384 Cuts at: 6222 6490 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1029 963 490 467 268 144 62 51 AflII C'TTAA_G Cuts at: 820 1017 3353 820 Size: 197 2336 4242 Fragments arranged by size: 4242 2336 197 AflIII A'CryG_T Cuts at: 1203 5674 6651 1203 Size: 4471 977 1327 Fragments arranged by size: 4471 1327 977 AgeI A'CCGG_T Cuts at: 5606 5606 Size: 6775 AhdI GACnn_n'nnGTC Cuts at: 5504 5504 Size: 6775 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6775 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6775 AlwNI CAG_nnn'CTG Cuts at: 3955 4518 6090 3955 Size: 563 1572 4640 Fragments arranged by size: 4640 1572 563 ApaLI G'TGCA_C Cuts at: 2115 4832 5988 2115 Size: 2717 1156 2902 Fragments arranged by size: 2902 2717 1156 ApoI r'AATT_y Cuts at: 1493 2832 3055 3842 4084 4310 4462 6571 1493 Size: 1339 223 787 242 226 152 2109 1697 Fragments arranged by size: 2109 1697 1339 787 242 226 223 152 AseI AT'TA_AT Cuts at: 160 1032 5329 160 Size: 872 4297 1606 Fragments arranged by size: 4297 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 AvaI C'yCGr_G Cuts at: 1870 1943 3860 4100 1870 Size: 73 1917 240 4545 Fragments arranged by size: 4545 1917 240 73 AvrII C'CTAG_G Cuts at: 2509 2509 Size: 6775 BamHI G'GATC_C Cuts at: 4105 4472 4105 Size: 367 6408 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4096 5551 618 Size: 325 217 627 2309 1455 1842 Fragments arranged by size: 2309 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3392 3672 3703 3861 4094 1391 Size: 2001 280 31 158 233 4072 Fragments arranged by size: 4072 2001 280 233 158 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2283 928 Size: 971 384 5420 Fragments arranged by size: 5420 971 384 BbvCI CC'TCA_GC Cuts at: 2913 2913 Size: 6775 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4966 5000 1903 Size: 34 3029 34 3678 Fragments arranged by size: 3678 3029 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3643 4667 5877 3643 Size: 1024 1210 4541 Fragments arranged by size: 4541 1210 1024 BclI T'GATC_A Cuts at: 1548 1548 Size: 6775 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5386 136 Size: 107 122 71 631 605 3714 1525 Fragments arranged by size: 3714 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6770 6770 Size: 6775 BlpI GC'TnA_GC Cuts at: 2866 4192 2866 Size: 1326 5449 Bme1580I G_kGCm'C Cuts at: 1790 2119 4836 5992 1790 Size: 329 2717 1156 2573 Fragments arranged by size: 2717 2573 1156 329 BmgBI CAC'GTC Cuts at: 1318 2154 1318 Size: 836 5939 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4506 5464 442 Size: 416 53 83 399 24 3089 958 1753 Fragments arranged by size: 3089 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6775 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2220 2925 4031 5435 1239 Size: 981 705 1106 1404 2579 Fragments arranged by size: 2579 1404 1106 981 705 Bpu10I CC'TnA_GC Cuts at: 2913 2913 Size: 6775 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2263 3645 4900 5765 6063 2263 Size: 1382 1255 865 298 2975 Fragments arranged by size: 2975 1382 1255 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2751 5438 882 Size: 617 1252 2687 2219 Fragments arranged by size: 2687 2219 1252 617 BsaAI yAC'GTr Cuts at: 493 1204 4566 493 Size: 711 3362 2702 Fragments arranged by size: 3362 2702 711 BsaBI GATnn'nnATC Cuts at: 3996 6769 3996 Size: 2773 4002 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4582 4964 5599 275 Size: 53 83 186 936 3049 382 635 1451 Fragments arranged by size: 3049 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5208 5606 5880 6027 6438 5208 Size: 398 274 147 411 5545 Fragments arranged by size: 5545 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6550 Fragments arranged by size: 6550 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3189 3534 1186 Size: 2003 345 4427 Fragments arranged by size: 4427 2003 345 BseYI C'CCAG_C Cuts at: 2716 3590 5978 6474 2716 Size: 874 2388 496 3017 Fragments arranged by size: 3017 2388 874 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3552 1163 Size: 573 1816 4386 Fragments arranged by size: 4386 1816 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4225 4986 6014 1540 Size: 275 186 2224 761 1028 2301 Fragments arranged by size: 2301 2224 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2119 2959 3310 3861 4836 4921 5992 2119 Size: 840 351 551 975 85 1071 2902 Fragments arranged by size: 2902 1071 975 840 551 351 85 BsmI GAATG_Cn' Cuts at: 4286 4379 4286 Size: 93 6682 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2119 2959 3310 3392 3672 3703 3861 Size: 399 329 840 351 82 280 31 158 Cuts at: 3861 4094 4836 4921 5992 1391 Size: 233 742 85 1071 2174 Fragments arranged by size: 2174 1071 840 742 399 351 329 280 233 158 85 82 31 BspEI T'CCGG_A Cuts at: 6438 6438 Size: 6775 BspHI T'CATG_A Cuts at: 4664 6394 4664 Size: 1730 5045 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4130 844 Size: 3286 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4662 1394 Size: 429 2839 3507 Fragments arranged by size: 3507 2839 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2889 2901 3927 5270 5444 65 Size: 1183 1641 12 1026 1343 174 1396 Fragments arranged by size: 1641 1396 1343 1183 1026 174 12 BsrFI r'CCGG_y Cuts at: 1700 1810 5419 5606 1700 Size: 110 3609 187 2869 Fragments arranged by size: 3609 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3162 96 Size: 3066 3709 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6775 BssSI C'ACGA_G Cuts at: 4835 5847 4835 Size: 1012 5763 BstAPI GCAn_nnn'nTGC Cuts at: 794 3955 794 Size: 3161 3614 BstBI TT'CG_AA Cuts at: 5579 5579 Size: 6775 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6775 BstXI CCAn_nnnn'nTGG Cuts at: 2896 2896 Size: 6775 BstYI r'GATC_y Cuts at: 1972 4105 4472 4857 4874 6326 6412 6424 6770 Size: 2133 367 385 17 1452 86 12 346 Cuts at: 6770 1972 Size: 1977 Fragments arranged by size: 2133 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3128 513 Size: 552 2063 4160 Fragments arranged by size: 4160 2063 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6036 Fragments arranged by size: 6036 683 56 BtsI GCAGTG_nn' Cuts at: 3953 4291 5085 5105 3953 Size: 338 794 20 5623 Fragments arranged by size: 5623 794 338 20 ClaI AT'CG_AT Cuts at: 1554 2948 1554 Size: 1394 5381 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2642 2677 529 Size: 35 2078 35 4627 Fragments arranged by size: 4627 2078 35 35 DraI TTT'AAA Cuts at: 3886 4079 3886 Size: 193 6582 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2246 5782 809 Size: 507 930 3536 1802 Fragments arranged by size: 3536 1802 930 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4222 5111 6740 Size: 54 1425 50 275 18 2392 889 1629 Cuts at: 6740 8 Size: 43 Fragments arranged by size: 2392 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4222 1537 Size: 275 2410 4090 Fragments arranged by size: 4090 2410 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2608 2931 3660 3705 4705 1353 Size: 1255 323 729 45 1000 3423 Fragments arranged by size: 3423 1255 1000 729 323 45 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5362 5736 5882 387 Size: 1064 138 3773 374 146 1280 Fragments arranged by size: 3773 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3859 3859 Size: 6775 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2122 2220 2589 2925 3618 3669 3813 Size: 393 490 98 369 336 693 51 144 Cuts at: 3813 3875 4031 4838 5435 6222 6490 1239 Size: 62 156 807 597 787 268 1524 Fragments arranged by size: 1524 807 787 693 597 490 393 369 336 268 156 144 98 62 51 EcoNI CCTnn'n_nnAGG Cuts at: 3516 3600 3516 Size: 84 6691 EcoO109I rG'GnC_Cy Cuts at: 3524 3524 Size: 6775 EcoRI G'AATT_C Cuts at: 2832 4084 4462 2832 Size: 1252 378 5145 Fragments arranged by size: 5145 1252 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2152 2152 Size: 6775 FspI TGC'GCA Cuts at: 5281 5281 Size: 6775 HaeII r_GCGC'y Cuts at: 1678 5922 1678 Size: 4244 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2617 2649 2930 2962 3454 Size: 32 135 32 1045 32 281 32 492 Cuts at: 3454 3486 3617 3649 4010 4042 5416 5448 5490 Size: 32 131 32 361 32 1374 32 42 Cuts at: 5490 5522 1373 Size: 32 2626 Fragments arranged by size: 2626 1374 1045 492 361 281 135 131 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3439 4119 4365 669 Size: 988 281 1501 680 246 3079 Fragments arranged by size: 3079 1501 988 680 281 246 HindIII A'AGCT_T Cuts at: 748 2613 3907 748 Size: 1865 1294 3616 Fragments arranged by size: 3616 1865 1294 HpaI GTT'AAC Cuts at: 4365 4365 Size: 6775 KpnI G_GTAC'C Cuts at: 4100 4100 Size: 6775 MfeI C'AATT_G Cuts at: 3221 4374 3221 Size: 1153 5622 MluI A'CGCG_T Cuts at: 6651 6651 Size: 6775 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5889 6073 1305 Size: 4584 184 2007 Fragments arranged by size: 4584 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4953 Fragments arranged by size: 4953 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2894 4733 5092 5251 518 Size: 648 1728 1839 359 159 2042 Fragments arranged by size: 2042 1839 1728 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4191 4868 6016 6261 1606 Size: 2585 677 1148 245 2120 Fragments arranged by size: 2585 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6223 NdeI CA'TA_TG Cuts at: 387 387 Size: 6775 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6775 NotI GC'GGCC_GC Cuts at: 4222 4222 Size: 6775 NspI r_CATG'y Cuts at: 1132 1229 4133 5678 1132 Size: 97 2904 1545 2229 Fragments arranged by size: 2904 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6775 PciI A'CATG_T Cuts at: 5674 5674 Size: 6775 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6775 PmeI GTTT'AAAC Cuts at: 4079 4079 Size: 6775 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4844 4876 6357 6389 4844 Size: 32 1481 32 5230 Fragments arranged by size: 5230 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6775 PsiI TTA'TAA Cuts at: 3825 4345 3825 Size: 520 6255 PstI C_TGCA'G Cuts at: 830 4127 830 Size: 3297 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6775 SacI G_AGCT'C Cuts at: 3861 3861 Size: 6775 SalI G'TCGA_C Cuts at: 1936 4117 1936 Size: 2181 4594 SapI GCTCTTCn'nnn_ Cuts at: 2931 3660 2931 Size: 729 6046 SbfI CC_TGCA'GG Cuts at: 4127 4127 Size: 6775 ScaI AGT'ACT Cuts at: 5023 6735 5023 Size: 1712 5063 SfcI C'TryA_G Cuts at: 826 1045 2028 2767 3311 4123 5258 5939 6130 Size: 219 983 739 544 812 1135 681 191 Cuts at: 6130 826 Size: 1471 Fragments arranged by size: 1471 1135 983 812 739 681 544 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6775 SmaI CCC'GGG Cuts at: 1872 4102 1872 Size: 2230 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2278 3353 3660 3860 4879 5780 Size: 197 926 335 1075 307 200 1019 901 Cuts at: 5780 6042 820 Size: 262 1553 Fragments arranged by size: 1553 1075 1019 926 901 335 307 262 200 197 SnaBI TAC'GTA Cuts at: 493 4566 493 Size: 4073 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6775 SphI G_CATG'C Cuts at: 1132 4133 1132 Size: 3001 3774 SspI AAT'ATT Cuts at: 5 52 2266 3073 4699 5 Size: 47 2214 807 1626 2081 Fragments arranged by size: 2214 2081 1626 807 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6775 StyI C'CwwG_G Cuts at: 513 1065 2509 3278 4214 513 Size: 552 1444 769 936 3074 Fragments arranged by size: 3074 1444 936 769 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3351 4802 4819 4972 5157 5602 325 Size: 1255 1771 1451 17 153 185 445 1498 Fragments arranged by size: 1771 1498 1451 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2608 3020 3094 Size: 274 80 33 51 1443 631 412 74 Cuts at: 3094 3155 3162 5021 6733 96 Size: 61 7 1859 1712 138 Fragments arranged by size: 1859 1712 1443 631 412 274 138 80 74 61 51 33 7 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2394 2426 6735 6767 2394 Size: 32 4309 32 2402 Fragments arranged by size: 4309 2402 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6775 XbaI T'CTAG_A Cuts at: 3296 4111 3296 Size: 815 5960 XhoI C'TCGA_G Cuts at: 1943 3860 1943 Size: 1917 4858 XmaI C'CCGG_G Cuts at: 1870 4100 1870 Size: 2230 4545 XmnI GAAnn'nnTTC Cuts at: 2602 3450 3681 4904 2602 Size: 848 231 1223 4473 Fragments arranged by size: 4473 1223 848 231 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4583 276 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI