################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII Alw44I AlwI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BmyI Bpu1102I BsaBI BsaHI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraII DraIII DsaI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MboI MfeI MflI MluI MroI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AflIII 378 ACACGT A^C[GA][CT]GT AgeI 526 ACCGGT A^CCGGT AhdI 196 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 79 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 269 AGCGCT AGC^GCT AspEI 196 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 225 GGACC G^G[AT]CC BalI 261 TGGCCA TGG^CCA BbrPI 379 CACGTG CAC^GTG BbsI 126 GAAGAC GAAGAC(2/6) BfaI 290 CTAG C^TAG BglI 172 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 52 CTGGAG CTGGAG(16/14) BpuAI 126 GAAGAC GAAGAC(2/6) BsaAI 379 CACGTG [CT]AC^GT[GA] BsaI 511 GAGACC GGTCTC(1/5) BsaJI 108 CCTGGG C^C[ACGT][ACGT]GG BsaWI 526 ACCGGT [AT]^CCGG[AT] BseRI 637 GAGGAG GAGGAG(10/8) Bsp1407I 152 TGTACA T^GTACA BsrBI 120 GAGCGG CCGCTC(-3/-3) BsrFI 526 ACCGGT [GA]^CCGG[CT] BsrGI 152 TGTACA T^GTACA BstXI 212 CCATGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 526 ACCGGT [GA]^CCGG[CT] Cfr13I 225 GGACC G^G[ACGT]CC DdeI 206 CTAAG C^T[ACGT]AG DrdI 400 GACGATGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 261 TGGCCA [CT]^GGCC[GA] Eam1105I 196 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 269 AGCGCT AGC^GCT FauI 122 GCGGG CCCGC(4/6) HaeII 269 AGCGCT [GA]GCGC^[CT] MaeI 290 CTAG C^TAG MluNI 261 TGGCCA TGG^CCA MscI 261 TGGCCA TGG^CCA MseI 353 TTAA T^TAA PinAI 526 ACCGGT A^CCGGT PleI 640 GAGTC GAGTC(4/5) PmaCI 379 CACGTG CAC^GTG PmlI 379 CACGTG CAC^GTG Sau96I 225 GGACC G^G[ACGT]CC SspBI 152 TGTACA T^GTACA StuI 23 AGGCCT AGG^CCT Tru9I 353 TTAA T^TAA XcmI 177 CCAGGCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AciI 144 CCGC CCGC(-3/-1) 122 GCGG AfaI 153 GTAC GT^AC 216 GTAC BsiYI 552 CCGGCGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 598 CCAAAAGAGGG BslI 552 CCGGCGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 598 CCAAAAGAGGG BsmFI 251 GGGAC GGGAC(10/14) 607 GGGAC BstF5I 131 CATCC GGATG(2/0) 29 GGATG CfoI 170 GCGC GCG^C 270 GCGC FokI 131 CATCC GGATG(9/13) 29 GGATG HhaI 170 GCGC GCG^C 270 GCGC HinP1I 170 GCGC G^CGC 270 GCGC MaeII 380 ACGT A^CGT 536 ACGT MboII 126 GAAGA GAAGA(8/7) 475 GAAGA MspA1I 238 CAGCTG C[AC]G^C[GT]G 257 CAGCTG PstI 241 CTGCAG CTGCA^G 461 CTGCAG PvuII 238 CAGCTG CAG^CTG 257 CAGCTG RsaI 153 GTAC GT^AC 216 GTAC SfcI 241 CTGCAG C^T[GA][CT]AG 461 CTGCAG TaqI 620 TCGA T^CGA 635 TCGA TfiI 304 GATTC G^A[AT]TC 628 GAATC TthHB8I 620 TCGA T^CGA 635 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AluI BbvI BsmAI BsrI BssKI BstNI Cac8I EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MnlI MspI MvaI MwoI NlaIII ScrFI TseI Tsp45I Tsp509I TspRI