(Linear) (Six-base) MAPSORT of: KIEE3517.seq  Check: 92  from: 1  to: 663



KIEE3517.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:35  ..

AfeI AGC'GCT

Cuts at:      0     271     663 
   Size:        271     392
 
AflIII A'CryG_T

Cuts at:      0     378     663 
   Size:        378     285
 
AgeI A'CCGG_T

Cuts at:      0     526     663 
   Size:        526     137
 
AhdI GACnn_n'nnGTC

Cuts at:      0     201     663 
   Size:        201     462
 
AlwNI CAG_nnn'CTG

Cuts at:      0      84     663 
   Size:         84     579
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     133     663 
   Size:        133     530
 
BglI GCCn_nnn'nGGC

Cuts at:      0     178     663 
   Size:        178     485
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     663 
   Size:         73     590
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     505     663 
   Size:        505     158
 
BsaAI yAC'GTr

Cuts at:      0     381     663 
   Size:        381     282
 
BsaWI w'CCGG_w

Cuts at:      0     526     663 
   Size:        526     137
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     576     606     663 
   Size:        576      30      57

  Fragments arranged by size: 

                 576      57      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     652     663 
   Size:        652      11
 
BsrBI CCG'CTC

Cuts at:      0     122     663 
   Size:        122     541
 
BsrFI r'CCGG_y

Cuts at:      0     526     663 
   Size:        526     137
 
BsrGI T'GTAC_A

Cuts at:      0     152     663 
   Size:        152     511
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     219     663 
   Size:        219     444
 
BtsI GCAGTG_nn'

Cuts at:      0     323     458     663 
   Size:        323     135     205

  Fragments arranged by size: 

                 323     205     135
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     406     663 
   Size:        406     257
 
EaeI y'GGCC_r

Cuts at:      0     261     663 
   Size:        261     402
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     663 
   Size:         73     590
 
HaeII r_GCGC'y

Cuts at:      0     273     663 
   Size:        273     390
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     187     219     576     608     663 
   Size:        187      32     357      32      55

  Fragments arranged by size: 

                 357     187      55      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     168     663 
   Size:        168     495
 
MscI TGG'CCA

Cuts at:      0     263     663 
   Size:        263     400
 
MspA1I CmG'CkG

Cuts at:      0     240     259     663 
   Size:        240      19     404

  Fragments arranged by size: 

                 404     240      19
 
PmlI CAC'GTG

Cuts at:      0     381     663 
   Size:        381     282
 
PstI C_TGCA'G

Cuts at:      0     245     465     663 
   Size:        245     220     198

  Fragments arranged by size: 

                 245     220     198
 
PvuII CAG'CTG

Cuts at:      0     240     259     663 
   Size:        240      19     404

  Fragments arranged by size: 

                 404     240      19
 
SfcI C'TryA_G

Cuts at:      0     241     461     663 
   Size:        241     220     202

  Fragments arranged by size: 

                 241     220     202
 
StuI AGG'CCT

Cuts at:      0      25     663 
   Size:         25     638
 
TatI w'GTAC_w

Cuts at:      0     152     215     663 
   Size:        152      63     448

  Fragments arranged by size: 

                 448     152      63
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     184     663 
   Size:        184     479
 

 Enzymes that do cut:

     AfeI   AflIII     AgeI     AhdI    AlwNI     BbsI     BglI     BpmI
     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BsrBI    BsrFI    BsrGI
    BstXI     BtsI     DrdI     EaeI  Eco57MI    HaeII    Hin4I     MmeI
     MscI   MspA1I     PmlI     PstI    PvuII     SfcI     StuI     TatI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsaHI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI