################################## # NO CUT ENZYMES ( 231 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TfiI Tru9I TseI Tsp509I TspRI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 21 enzymes) # ################################### AspI 97 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanII 42 GAGCCC G[GA]GC[CT]^C BbsI 171 GAAGAC GAAGAC(2/6) BmyI 42 GAGCCC G[AGT]GC[ACT]^C BpmI 199 CTGGAG CTGGAG(16/14) BpuAI 171 GAAGAC GAAGAC(2/6) BsmAI 82 GAGAC GTCTC(1/5) BsmFI 95 GGGAC GGGAC(10/14) Bsp1286I 42 GAGCCC G[AGT]GC[ACT]^C HinfI 52 GACTC G^A[ACGT]TC MaeIII 50 GTGAC ^GT[ACGT]AC PflFI 97 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 52 GACTC GAGTC(4/5) SapI 39 GAAGAGC GCTCTTC(1/4) SfaNI 75 GATGC GCATC(5/9) SfcI 165 CTACAG C^T[GA][CT]AG SspI 140 AATATT AAT^ATT TaqI 107 TCGA T^CGA Tsp45I 50 GTGAC ^GT[GC]AC Tth111I 97 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 107 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 5 enzymes) # ################################### BsrI 198 ACTGG ACTGG(1/-1) 210 CCAGT DdeI 99 CTTAG C^T[ACGT]AG 176 CTAAG EarI 33 GAAGAG CTCTTC(1/4) 39 GAAGAG Ksp632I 33 GAAGAG CTCTTC(1/4) 39 GAAGAG MnlI 105 CCTC CCTC(7/6) 36 GAGG ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### MboII