(Circular) (Six-base) MAPSORT of: pFN21AE1722.seq Check: 8932 from: 1 to: 5404 pFN21AE1722 5404 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2687 2687 Size: 5404 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3214 278 Size: 53 83 186 936 1678 2468 Fragments arranged by size: 2468 1678 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2747 1937 Size: 810 4594 Acc65I G'GTAC_C Cuts at: 2725 2725 Size: 5404 AclI AA'CG_TT Cuts at: 1563 3531 3904 1563 Size: 1968 373 3063 Fragments arranged by size: 3063 1968 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3467 4851 5119 1632 Size: 1835 1384 268 1917 Fragments arranged by size: 1917 1835 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5207 AflIII A'CryG_T Cuts at: 1203 4303 5280 1203 Size: 3100 977 1327 Fragments arranged by size: 3100 1327 977 AgeI A'CCGG_T Cuts at: 4235 4235 Size: 5404 AhdI GACnn_n'nnGTC Cuts at: 2380 4133 2380 Size: 1753 3651 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5404 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2135 1184 Size: 951 4453 AlwNI CAG_nnn'CTG Cuts at: 2332 3147 4719 2332 Size: 815 1572 3017 Fragments arranged by size: 3017 1572 815 ApaI G_GGCC'C Cuts at: 2317 2423 2530 2317 Size: 106 107 5191 Fragments arranged by size: 5191 107 106 ApaLI G'TGCA_C Cuts at: 3461 4617 3461 Size: 1156 4248 ApoI r'AATT_y Cuts at: 1493 2713 2939 3091 5200 1493 Size: 1220 226 152 2109 1697 Fragments arranged by size: 2109 1697 1220 226 152 AseI AT'TA_AT Cuts at: 160 1032 3958 160 Size: 872 2926 1606 Fragments arranged by size: 2926 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5404 AvaI C'yCGr_G Cuts at: 1870 1943 2729 1870 Size: 73 786 4545 Fragments arranged by size: 4545 786 73 BamHI G'GATC_C Cuts at: 2734 3101 2734 Size: 367 5037 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 2147 2664 2725 4180 Size: 325 217 627 240 120 517 61 1455 Cuts at: 4180 618 Size: 1842 Fragments arranged by size: 1842 1455 627 517 325 240 217 120 61 BanII G_rGCy'C Cuts at: 1391 2060 2089 2317 2423 2530 2634 2723 1391 Size: 669 29 228 106 107 104 89 4072 Fragments arranged by size: 4072 669 228 107 106 104 89 29 BbeI G_GCGC'C Cuts at: 2151 2151 Size: 5404 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2353 2663 928 Size: 971 454 310 3669 Fragments arranged by size: 3669 971 454 310 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3595 3629 1903 Size: 34 1658 34 3678 Fragments arranged by size: 3678 1658 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3296 4506 3296 Size: 1210 4194 BclI T'GATC_A Cuts at: 1548 1548 Size: 5404 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2688 4015 136 Size: 107 122 71 631 605 1016 1327 1525 Fragments arranged by size: 1525 1327 1016 631 605 122 107 71 BglII A'GATC_T Cuts at: 5399 5399 Size: 5404 BlpI GC'TnA_GC Cuts at: 2821 2821 Size: 5404 Bme1580I G_kGCm'C Cuts at: 1790 2317 2423 2530 3465 4621 1790 Size: 527 106 107 935 1156 2573 Fragments arranged by size: 2573 1156 935 527 107 106 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5404 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3135 4093 442 Size: 416 53 83 399 24 1718 958 1753 Fragments arranged by size: 1753 1718 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5404 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2502 4064 1239 Size: 1263 1562 2579 Fragments arranged by size: 2579 1562 1263 Bpu10I CC'TnA_GC Cuts at: 2088 2088 Size: 5404 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3529 4394 4692 3529 Size: 865 298 4241 Fragments arranged by size: 4241 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2534 4067 882 Size: 617 1035 1533 2219 Fragments arranged by size: 2219 1533 1035 617 BsaAI yAC'GTr Cuts at: 493 1204 3195 493 Size: 711 1991 2702 Fragments arranged by size: 2702 1991 711 BsaBI GATnn'nnATC Cuts at: 5398 5398 Size: 5404 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 2358 3211 3593 Size: 53 83 186 936 615 210 853 382 Cuts at: 3593 4228 275 Size: 635 1451 Fragments arranged by size: 1451 936 853 635 615 382 210 186 83 53 BsaWI w'CCGG_w Cuts at: 3837 4235 4509 4656 5067 3837 Size: 398 274 147 411 4174 Fragments arranged by size: 4174 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5179 Fragments arranged by size: 5179 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2585 1186 Size: 1399 4005 BseYI C'CCAG_C Cuts at: 2353 2433 2520 4607 5103 2353 Size: 80 87 2087 496 2654 Fragments arranged by size: 2654 2087 496 87 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4831 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2854 3615 4643 1540 Size: 275 186 853 761 1028 2301 Fragments arranged by size: 2301 1028 853 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3465 3550 4621 3465 Size: 85 1071 4248 Fragments arranged by size: 4248 1071 85 BsmI GAATG_Cn' Cuts at: 2271 2915 3008 2271 Size: 644 93 4667 Fragments arranged by size: 4667 644 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2388 2388 Size: 5404 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2089 2317 2423 2530 2634 2723 Size: 399 270 29 228 106 107 104 89 Cuts at: 2723 3465 3550 4621 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 270 228 107 106 104 89 85 29 BspEI T'CCGG_A Cuts at: 5067 5067 Size: 5404 BspHI T'CATG_A Cuts at: 3293 5023 3293 Size: 1730 3674 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2687 2759 844 Size: 1843 72 3489 Fragments arranged by size: 3489 1843 72 BsrBI CCG'CTC Cuts at: 1394 1823 3291 1394 Size: 429 1468 3507 Fragments arranged by size: 3507 1468 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3899 4073 65 Size: 1183 2651 174 1396 Fragments arranged by size: 2651 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4048 4235 1700 Size: 110 2238 187 2869 Fragments arranged by size: 2869 2238 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5404 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5404 BssSI C'ACGA_G Cuts at: 2447 3464 4476 2447 Size: 1017 1012 3375 Fragments arranged by size: 3375 1017 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5404 BstBI TT'CG_AA Cuts at: 4208 4208 Size: 5404 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5404 BstXI CCAn_nnnn'nTGG Cuts at: 2158 2158 Size: 5404 BstYI r'GATC_y Cuts at: 1972 2598 2734 3101 3486 3503 4955 5041 5053 Size: 626 136 367 385 17 1452 86 12 Cuts at: 5053 5399 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 626 385 367 346 136 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2619 2634 513 Size: 552 939 615 15 3283 Fragments arranged by size: 3283 939 615 552 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4665 Fragments arranged by size: 4665 683 56 BtsI GCAGTG_nn' Cuts at: 2475 2920 3714 3734 2475 Size: 445 794 20 4145 Fragments arranged by size: 4145 794 445 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5404 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5369 DraI TTT'AAA Cuts at: 2708 2708 Size: 5404 DraIII CAC_nnn'GTG Cuts at: 2536 2536 Size: 5404 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4411 809 Size: 507 3095 1802 Fragments arranged by size: 3095 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2547 2700 Size: 54 1425 50 275 18 216 501 153 Cuts at: 2700 2851 3740 5369 8 Size: 151 889 1629 43 Fragments arranged by size: 1629 1425 889 501 275 216 153 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2851 1537 Size: 275 1039 4090 Fragments arranged by size: 4090 1039 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3334 1353 Size: 1981 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3991 4365 4511 387 Size: 1064 138 2402 374 146 1280 Fragments arranged by size: 2402 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2502 3467 4064 4851 5119 1239 Size: 393 870 965 597 787 268 1524 Fragments arranged by size: 1524 965 870 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2313 2314 2419 2420 2526 2643 2313 Size: 1 105 1 106 117 5074 Fragments arranged by size: 5074 117 106 105 1 1 EcoRI G'AATT_C Cuts at: 2713 3091 2713 Size: 378 5026 FspI TGC'GCA Cuts at: 3910 3910 Size: 5404 HaeII r_GCGC'y Cuts at: 1678 2151 4551 1678 Size: 473 2400 2531 Fragments arranged by size: 2531 2400 473 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2366 2395 2398 4045 Size: 32 135 32 791 3 29 3 1647 Cuts at: 4045 4077 4119 4151 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1647 791 135 42 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2250 2748 2994 669 Size: 988 281 312 498 246 3079 Fragments arranged by size: 3079 988 498 312 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5404 HpaI GTT'AAC Cuts at: 2994 2994 Size: 5404 KasI G'GCGC_C Cuts at: 2147 2147 Size: 5404 KpnI G_GTAC'C Cuts at: 2729 2729 Size: 5404 MfeI C'AATT_G Cuts at: 3003 3003 Size: 5404 MluI A'CGCG_T Cuts at: 5280 5280 Size: 5404 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4518 4702 1305 Size: 3213 184 2007 Fragments arranged by size: 3213 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 10 Size: 54 1425 343 216 3366 Fragments arranged by size: 3366 1425 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 3362 3721 3880 518 Size: 648 2196 359 159 2042 Fragments arranged by size: 2196 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2119 2185 2395 2498 2520 2820 3497 4645 Size: 513 66 210 103 22 300 677 1148 Cuts at: 4645 4890 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 513 300 245 210 103 66 22 NarI GG'CG_CC Cuts at: 2148 2148 Size: 5404 NcoI C'CATG_G Cuts at: 513 1065 2004 2619 2634 513 Size: 552 939 615 15 3283 Fragments arranged by size: 3283 939 615 552 15 NdeI CA'TA_TG Cuts at: 387 387 Size: 5404 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5404 NotI GC'GGCC_GC Cuts at: 2851 2851 Size: 5404 NspI r_CATG'y Cuts at: 1132 1229 2762 4307 1132 Size: 97 1533 1545 2229 Fragments arranged by size: 2229 1545 1533 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5404 PciI A'CATG_T Cuts at: 4303 4303 Size: 5404 PflMI CCAn_nnn'nTGG Cuts at: 1108 2332 2536 1108 Size: 1224 204 3976 Fragments arranged by size: 3976 1224 204 PfoI T'CCnGG_A Cuts at: 2226 2610 2226 Size: 384 5020 PmeI GTTT'AAAC Cuts at: 2708 2708 Size: 5404 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3473 3505 4986 5018 3473 Size: 32 1481 32 3859 Fragments arranged by size: 3859 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2643 2643 Size: 5404 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5404 PsiI TTA'TAA Cuts at: 2974 2974 Size: 5404 PspOMI G'GGCC_C Cuts at: 2313 2419 2526 2313 Size: 106 107 5191 Fragments arranged by size: 5191 107 106 PstI C_TGCA'G Cuts at: 830 2400 2506 2756 830 Size: 1570 106 250 3478 Fragments arranged by size: 3478 1570 250 106 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5404 PvuII CAG'CTG Cuts at: 2119 2185 2395 2520 2119 Size: 66 210 125 5003 Fragments arranged by size: 5003 210 125 66 SalI G'TCGA_C Cuts at: 1936 2746 1936 Size: 810 4594 SbfI CC_TGCA'GG Cuts at: 2756 2756 Size: 5404 ScaI AGT'ACT Cuts at: 3652 5364 3652 Size: 1712 3692 SfcI C'TryA_G Cuts at: 826 1045 2396 2502 2647 2752 3887 4568 4759 Size: 219 1351 106 145 105 1135 681 191 Cuts at: 4759 826 Size: 1471 Fragments arranged by size: 1471 1351 1135 681 219 191 145 106 105 SfoI GGC'GCC Cuts at: 2149 2149 Size: 5404 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5404 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5404 SmaI CCC'GGG Cuts at: 1872 2731 1872 Size: 859 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3508 4409 4671 820 Size: 197 926 1565 901 262 1553 Fragments arranged by size: 1565 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3195 493 Size: 2702 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5404 SphI G_CATG'C Cuts at: 1132 2762 1132 Size: 1630 3774 SspI AAT'ATT Cuts at: 5 52 3328 5 Size: 47 3276 2081 Fragments arranged by size: 3276 2081 47 StuI AGG'CCT Cuts at: 1517 2576 1517 Size: 1059 4345 StyI C'CwwG_G Cuts at: 513 1065 2004 2619 2634 2843 513 Size: 552 939 615 15 209 3074 Fragments arranged by size: 3074 939 615 552 209 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3431 3448 3601 3786 4231 325 Size: 1255 1851 17 153 185 445 1498 Fragments arranged by size: 1851 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2127 3650 5362 Size: 274 80 33 51 1443 150 1523 1712 Cuts at: 5362 96 Size: 138 Fragments arranged by size: 1712 1523 1443 274 150 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5364 5396 5364 Size: 32 5372 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5404 XbaI T'CTAG_A Cuts at: 2740 2740 Size: 5404 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5404 XmaI C'CCGG_G Cuts at: 1870 2729 1870 Size: 859 4545 XmnI GAAnn'nnTTC Cuts at: 3533 3533 Size: 5404 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3212 276 Size: 53 83 186 936 1678 2468 Fragments arranged by size: 2468 1678 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI