################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AccI AccIII AclI AflII AgeI Aor51HI AscI AseI AsnI Asp700I AspI AviII AvrII BbeI BbrPI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DrdI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FspI HincII HindII HindIII HpaI KasI MamI MfeI MluI MroI MunI NarI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 787 GACGTC GACGT^C Acc65I 1320 GGTACC G^GTACC AcsI 1124 GAATTT [GA]^AATT[CT] AhdI 1659 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 907 GTGCAC G^TGCAC AocI 585 CCTGAGG CC^T[ACGT]AGG ApaI 341 GGGCCC GGGCC^C ApaLI 907 GTGCAC G^TGCAC ApoI 1124 GAATTT [GA]^AATT[CT] Asp718I 1320 GGTACC G^GTACC AspEI 1659 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1308 TGGCCA TGG^CCA BamHI 1166 GGATCC G^GATCC BbvCI 1339 GCTGAGG CCTCAGC(-5/-2) BclI 134 TGATCA T^GATCA BfaI 151 CTAG C^TAG BglI 583 GCCCTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1050 CACGTA [CT]AC^GT[GA] BsgI 264 CTGCAC GTGCAG(16/14) BspMI 655 ACCTGC ACCTGC(4/8) BsrDI 329 GCAATG GCAATG(2/0) Bsu36I 585 CCTGAGG CC^T[ACGT]AGG DraIII 1612 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1659 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 886 CTCTTC CTCTTC(1/4) Eco81I 585 CCTGAGG CC^T[ACGT]AGG FbaI 134 TGATCA T^GATCA FseI 1209 GGCCGGCC GGCCGG^CC HaeII 621 GGCGCT [GA]GCGC^[CT] KpnI 1320 GGTACC GGTAC^C Ksp632I 886 CTCTTC CTCTTC(1/4) MaeI 151 CTAG C^TAG MluNI 1308 TGGCCA TGG^CCA MscI 1308 TGGCCA TGG^CCA NotI 250 GCGGCCGC GC^GGCCGC PpuMI 1562 GGGACCC [GA]G^G[AT]CC[CT] PvuII 1267 CAGCTG CAG^CTG SmaI 1177 CCCGGG CCC^GGG SphI 524 GCATGC GCATG^C SspI 202 AATATT AAT^ATT TaqI 1019 TCGA T^CGA TthHB8I 1019 TCGA T^CGA XmaCI 1177 CCCGGG C^CCGGG XmaI 1177 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 88 ACGTGT A^C[GA][CT]GT 1247 ACGTGT AspHI 907 GTGCAC G[AT]GC[AT]^C 1201 GTGCTC AvaI 1177 CCCGGG C^[CT]CG[GA]G 1465 CCCGAG AvaII 1263 GGTCC G^G[AT]CC 1563 GGACC BbsI 84 GAAGAC GAAGAC(2/6) 1021 GAAGAC BcgI 824 CGAGTCTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1492 CGAGGCGTCTGC BciVI 334 GGATAC GTATCC(6/5) 767 GTATCC BpmI 1557 CTCCAG CTGGAG(16/14) 811 CTGGAG BpuAI 84 GAAGAC GAAGAC(2/6) 1021 GAAGAC BsiHKAI 907 GTGCAC G[AT]GC[AT]^C 1201 GTGCTC BsmAI 297 GAGAC GTCTC(1/5) 673 GTCTC BsmI 283 GCATTC GAATGC(1/-1) 934 GCATTC BsoBI 1177 CCCGGG C^[CT]CG[GA]G 1465 CCCGAG BsrBI 259 CCGCTC CCGCTC(-3/-3) 1472 GAGCGG BstXI 803 CCATGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1755 CCACGAGGATGG DraI 115 TTTAAA TTT^AAA 1032 TTTAAA EcoT14I 803 CCATGG C^C[AT][AT]GG 1068 CCATGG KspI 471 CCGCGG CCGC^GG 1637 CCGCGG NcoI 803 CCATGG C^CATGG 1068 CCATGG NspI 524 GCATGC [GA]CATG^[CT] 903 GCATGT PstI 1330 CTGCAG CTGCA^G 1717 CTGCAG SacII 471 CCGCGG CCGC^GG 1637 CCGCGG SfcI 1330 CTGCAG C^T[GA][CT]AG 1717 CTGCAG StyI 803 CCATGG C^C[AT][AT]GG 1068 CCATGG TfiI 401 GAATC G^A[AT]TC 630 GATTC Tsp509I 460 AATT ^AATT 1125 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI BanI BanII BbvI BcnI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I TspRI Van91I XcmI XhoII