(Circular) (Six-base) MAPSORT of: pFN21AE1259.seq Check: 9458 from: 1 to: 6484 pFN21AE1259 6484 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2796 4294 278 Size: 53 83 186 936 1260 1498 2468 Fragments arranged by size: 2468 1498 1260 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 1937 Size: 1890 4594 Acc65I G'GTAC_C Cuts at: 3325 3805 3325 Size: 480 6004 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 2296 2336 4547 5931 6199 1632 Size: 476 188 40 2211 1384 268 1917 Fragments arranged by size: 2211 1917 1384 476 268 188 40 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 2093 3252 5383 6360 1203 Size: 890 1159 2131 977 1327 Fragments arranged by size: 2131 1327 1159 977 890 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 3669 5213 3669 Size: 1544 4940 AleI CACnn'nnGTG Cuts at: 1166 2457 1166 Size: 1291 5193 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2442 1184 Size: 1258 5226 AlwNI CAG_nnn'CTG Cuts at: 2251 2623 3667 4227 5799 2251 Size: 372 1044 560 1572 2936 Fragments arranged by size: 2936 1572 1044 560 372 ApaI G_GGCC'C Cuts at: 2350 2350 Size: 6484 ApaLI G'TGCA_C Cuts at: 2912 4541 5697 2912 Size: 1629 1156 3699 Fragments arranged by size: 3699 1629 1156 ApoI r'AATT_y Cuts at: 1493 3129 3793 4019 4171 6280 1493 Size: 1636 664 226 152 2109 1697 Fragments arranged by size: 2109 1697 1636 664 226 152 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 3182 3470 3809 1870 Size: 73 1239 288 339 4545 Fragments arranged by size: 4545 1239 339 288 73 BamHI G'GATC_C Cuts at: 3171 3814 4181 3171 Size: 643 367 5474 Fragments arranged by size: 5474 643 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2451 3289 3325 3411 3638 Size: 325 217 627 664 838 36 86 227 Cuts at: 3638 3776 3805 5260 618 Size: 138 29 1455 1842 Fragments arranged by size: 1842 1455 838 664 627 325 227 217 138 86 36 29 BanII G_rGCy'C Cuts at: 1391 2350 3237 3609 3803 1391 Size: 959 887 372 194 4072 Fragments arranged by size: 4072 959 887 372 194 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2096 3033 928 Size: 971 197 937 4379 Fragments arranged by size: 4379 971 937 197 BbvCI CC'TCA_GC Cuts at: 3345 3345 Size: 6484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2818 2852 3486 3520 4675 Size: 34 50 34 797 34 634 34 1155 Cuts at: 4675 4709 1903 Size: 34 3678 Fragments arranged by size: 3678 1155 797 634 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2333 2783 4376 5586 2333 Size: 450 1593 1210 3231 Fragments arranged by size: 3231 1593 1210 450 BclI T'GATC_A Cuts at: 1548 2139 1548 Size: 591 5893 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2594 5095 136 Size: 107 122 71 631 605 922 2501 1525 Fragments arranged by size: 2501 1525 922 631 605 122 107 71 BglII A'GATC_T Cuts at: 6479 6479 Size: 6484 BlpI GC'TnA_GC Cuts at: 3901 3901 Size: 6484 Bme1580I G_kGCm'C Cuts at: 1790 2350 2916 3183 3294 3681 4545 5701 1790 Size: 560 566 267 111 387 864 1156 2573 Fragments arranged by size: 2573 1156 864 566 560 387 267 111 BmgBI CAC'GTC Cuts at: 1318 2094 3253 1318 Size: 776 1159 4549 Fragments arranged by size: 4549 1159 776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2172 2611 4215 Size: 416 53 83 399 24 755 439 1604 Cuts at: 4215 5173 442 Size: 958 1753 Fragments arranged by size: 1753 1604 958 755 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2259 3370 2259 Size: 1111 5373 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2837 3547 5144 1239 Size: 1598 710 1597 2579 Fragments arranged by size: 2579 1598 1597 710 Bpu10I CC'TnA_GC Cuts at: 2368 3345 2368 Size: 977 5507 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4609 5474 5772 4609 Size: 865 298 5321 Fragments arranged by size: 5321 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5147 882 Size: 617 3648 2219 Fragments arranged by size: 3648 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3057 4275 493 Size: 711 1853 1218 2702 Fragments arranged by size: 2702 1853 1218 711 BsaBI GATnn'nnATC Cuts at: 6478 6478 Size: 6484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2328 2583 2793 3501 Size: 53 83 186 936 795 255 210 708 Cuts at: 3501 4291 4673 5308 275 Size: 790 382 635 1451 Fragments arranged by size: 1451 936 795 790 708 635 382 255 210 186 83 53 BsaWI w'CCGG_w Cuts at: 4917 5315 5589 5736 6147 4917 Size: 398 274 147 411 5254 Fragments arranged by size: 5254 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6259 Fragments arranged by size: 6259 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2731 2879 2999 1186 Size: 1545 148 120 4671 Fragments arranged by size: 4671 1545 148 120 BseYI C'CCAG_C Cuts at: 2629 3681 5687 6183 2629 Size: 1052 2006 496 2930 Fragments arranged by size: 2930 2006 1052 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2254 1163 Size: 573 518 5393 Fragments arranged by size: 5393 573 518 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2259 3216 3220 3496 3934 4695 Size: 275 186 258 957 4 276 438 761 Cuts at: 4695 5723 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 957 761 438 276 275 258 186 4 BsiHKAI G_wGCw'C Cuts at: 2916 3210 4545 4630 5701 2916 Size: 294 1335 85 1071 3699 Fragments arranged by size: 3699 1335 1071 294 85 BsmI GAATG_Cn' Cuts at: 2288 2939 3995 4088 2288 Size: 651 1056 93 4684 Fragments arranged by size: 4684 1056 651 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2350 2916 3183 3210 3237 3294 3609 Size: 399 560 566 267 27 27 57 315 Cuts at: 3609 3681 3803 4545 4630 5701 1391 Size: 72 122 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 566 560 399 315 267 122 85 72 57 27 27 BspEI T'CCGG_A Cuts at: 6147 6147 Size: 6484 BspHI T'CATG_A Cuts at: 4373 6103 4373 Size: 1730 4754 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2669 3839 844 Size: 1825 1170 3489 Fragments arranged by size: 3489 1825 1170 BsrBI CCG'CTC Cuts at: 1394 1823 2266 3479 4371 1394 Size: 429 443 1213 892 3507 Fragments arranged by size: 3507 1213 892 443 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2341 4979 5153 65 Size: 1183 1093 2638 174 1396 Fragments arranged by size: 2638 1396 1183 1093 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 2432 2651 2696 2705 3215 5128 Size: 110 249 373 219 45 9 510 1913 Cuts at: 5128 5315 1700 Size: 187 2869 Fragments arranged by size: 2869 1913 510 373 249 219 187 110 45 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6484 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6484 BssSI C'ACGA_G Cuts at: 2932 3238 3761 4544 5556 2932 Size: 306 523 783 1012 3860 Fragments arranged by size: 3860 1012 783 523 306 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6484 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6484 BstXI CCAn_nnnn'nTGG Cuts at: 2815 3767 2815 Size: 952 5532 BstYI r'GATC_y Cuts at: 1972 2167 3171 3186 3814 4181 4566 4583 6035 Size: 195 1004 15 628 367 385 17 1452 Cuts at: 6035 6121 6133 6479 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1004 628 385 367 346 195 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 2591 2591 Size: 6484 BtgI C'CryG_G Cuts at: 513 1065 2476 2506 2808 3073 3642 3716 513 Size: 552 1411 30 302 265 569 74 3281 Fragments arranged by size: 3281 1411 569 552 302 265 74 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5745 Fragments arranged by size: 5745 683 56 BtsI GCAGTG_nn' Cuts at: 2485 4000 4794 4814 2485 Size: 1515 794 20 4155 Fragments arranged by size: 4155 1515 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 2122 3039 3788 2122 Size: 917 749 4818 Fragments arranged by size: 4818 917 749 DraIII CAC_nnn'GTG Cuts at: 3622 3622 Size: 6484 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5491 809 Size: 507 4175 1802 Fragments arranged by size: 4175 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 2653 2703 Size: 54 1425 50 275 18 426 397 50 Cuts at: 2703 3197 3213 3217 3313 3493 3931 4820 6449 Size: 494 16 4 96 180 438 889 1629 Cuts at: 6449 8 Size: 43 Fragments arranged by size: 1629 1425 889 494 438 426 397 275 180 96 54 50 50 43 18 16 4 EagI C'GGCC_G Cuts at: 1537 1812 2256 3213 3217 3493 3931 1537 Size: 275 444 957 4 276 438 4090 Fragments arranged by size: 4090 957 444 438 276 275 4 EarI CTCTTCn'nnn_ Cuts at: 1353 2897 4414 1353 Size: 1544 1517 3423 Fragments arranged by size: 3423 1544 1517 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2002 2162 5071 5445 5591 387 Size: 1064 138 413 160 2909 374 146 1280 Fragments arranged by size: 2909 1280 1064 413 374 160 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2296 2336 2837 3547 4547 5144 Size: 393 476 188 40 501 710 1000 597 Cuts at: 5144 5931 6199 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1000 787 710 597 501 476 393 268 188 40 EcoO109I rG'GnC_Cy Cuts at: 2347 2568 3483 3568 2347 Size: 221 915 85 5263 Fragments arranged by size: 5263 915 221 85 EcoRI G'AATT_C Cuts at: 3793 4171 3793 Size: 378 6106 FseI GG_CCGG'CC Cuts at: 3219 3219 Size: 6484 FspI TGC'GCA Cuts at: 4990 4990 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 2630 5631 1678 Size: 952 3001 2531 Fragments arranged by size: 3001 2531 952 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3655 3687 5125 5157 5199 Size: 32 135 32 2083 32 1438 32 42 Cuts at: 5199 5231 1373 Size: 32 2626 Fragments arranged by size: 2626 2083 1438 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3828 4074 669 Size: 988 281 1890 246 3079 Fragments arranged by size: 3079 1890 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KpnI G_GTAC'C Cuts at: 3329 3809 3329 Size: 480 6004 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3630 5598 5782 1305 Size: 2325 1968 184 2007 Fragments arranged by size: 2325 2007 1968 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3315 10 Size: 54 1425 343 1483 3179 Fragments arranged by size: 3179 1483 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2424 2457 2699 2874 3381 3765 4442 Size: 648 1258 33 242 175 507 384 677 Cuts at: 4442 4801 4960 518 Size: 359 159 2042 Fragments arranged by size: 2042 1258 677 648 507 384 359 242 175 159 33 MspA1I CmG'CkG Cuts at: 1606 2478 3274 3644 3900 4577 5725 5970 1606 Size: 872 796 370 256 677 1148 245 2120 Fragments arranged by size: 2120 1148 872 796 677 370 256 245 NaeI GCC'GGC Cuts at: 2653 2707 3217 2653 Size: 54 510 5920 Fragments arranged by size: 5920 510 54 NcoI C'CATG_G Cuts at: 513 1065 2808 3073 513 Size: 552 1743 265 3924 Fragments arranged by size: 3924 1743 552 265 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NgoMIV G'CCGG_C Cuts at: 2651 2705 3215 2651 Size: 54 510 5920 Fragments arranged by size: 5920 510 54 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 2256 3931 2256 Size: 1675 4809 NspI r_CATG'y Cuts at: 1132 1229 2533 2912 3842 5387 1132 Size: 97 1304 379 930 1545 2229 Fragments arranged by size: 2229 1545 1304 930 379 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6484 PciI A'CATG_T Cuts at: 5383 5383 Size: 6484 PflMI CCAn_nnn'nTGG Cuts at: 1108 2623 3277 3322 1108 Size: 1515 654 45 4270 Fragments arranged by size: 4270 1515 654 45 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2351 2383 4553 4585 6066 6098 2351 Size: 32 2170 32 1481 32 2737 Fragments arranged by size: 2737 2170 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3568 3568 Size: 6484 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PspOMI G'GGCC_C Cuts at: 2346 2346 Size: 6484 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3116 3148 3116 Size: 32 6452 PstI C_TGCA'G Cuts at: 830 3339 3726 3836 830 Size: 2509 387 110 3478 Fragments arranged by size: 3478 2509 387 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 3274 3274 Size: 6484 SacII CC_GC'GG Cuts at: 2479 3645 2479 Size: 1166 5318 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SanDI GG'GwC_CC Cuts at: 3568 3568 Size: 6484 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SfcI C'TryA_G Cuts at: 826 1045 3335 3722 3832 4967 5648 5839 826 Size: 219 2290 387 110 1135 681 191 1471 Fragments arranged by size: 2290 1471 1135 681 387 219 191 110 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 3184 3811 1872 Size: 1312 627 4545 Fragments arranged by size: 4545 1312 627 SmlI C'TyrA_G Cuts at: 820 1017 1943 4588 5489 5751 820 Size: 197 926 2645 901 262 1553 Fragments arranged by size: 2645 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 2533 3842 1132 Size: 1401 1309 3774 Fragments arranged by size: 3774 1401 1309 SspI AAT'ATT Cuts at: 5 52 2209 4408 5 Size: 47 2157 2199 2081 Fragments arranged by size: 2199 2157 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6484 StyI C'CwwG_G Cuts at: 513 1065 2808 3073 3923 513 Size: 552 1743 265 850 3074 Fragments arranged by size: 3074 1743 850 552 265 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2630 3269 4511 4528 4681 4866 5311 Size: 1255 1050 639 1242 17 153 185 445 Cuts at: 5311 325 Size: 1498 Fragments arranged by size: 1498 1255 1242 1050 639 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4730 6442 96 Size: 274 80 33 51 1443 2753 1712 138 Fragments arranged by size: 2753 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3351 3383 6444 6476 3351 Size: 32 3061 32 3359 Fragments arranged by size: 3359 3061 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6484 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3244 3278 3422 3244 Size: 34 144 6306 Fragments arranged by size: 6306 144 34 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6484 XmaI C'CCGG_G Cuts at: 1870 3182 3809 1870 Size: 1312 627 4545 Fragments arranged by size: 4545 1312 627 XmnI GAAnn'nnTTC Cuts at: 4613 4613 Size: 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2794 4292 276 Size: 53 83 186 936 1260 1498 2468 Fragments arranged by size: 2468 1498 1260 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI