Restriction map for
pFN21AE1259
(Linear) (Six-base) MAPSORT of: KIEE1259.seq Check: 6124 from: 1 to: 1779
KIEE1259.orf
With 182 enzymes: SgfI *
July 15, 2010 18:35 ..
AatII G_ACGT'C
Cuts at: 0 791 1779
Size: 791 988
Acc65I G'GTAC_C
Cuts at: 0 1320 1779
Size: 1320 459
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 291 331 1779
Size: 103 188 40 1448
Fragments arranged by size:
1448 188 103 40
AflIII A'CryG_T
Cuts at: 0 88 1247 1779
Size: 88 1159 532
Fragments arranged by size:
1159 532 88
AhdI GACnn_n'nnGTC
Cuts at: 0 1664 1779
Size: 1664 115
AleI CACnn'nnGTG
Cuts at: 0 452 1779
Size: 452 1327
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 437 1779
Size: 437 1342
AlwNI CAG_nnn'CTG
Cuts at: 0 246 618 1662 1779
Size: 246 372 1044 117
Fragments arranged by size:
1044 372 246 117
ApaI G_GGCC'C
Cuts at: 0 345 1779
Size: 345 1434
ApaLI G'TGCA_C
Cuts at: 0 907 1779
Size: 907 872
ApoI r'AATT_y
Cuts at: 0 1124 1779
Size: 1124 655
AvaI C'yCGr_G
Cuts at: 0 1177 1465 1779
Size: 1177 288 314
Fragments arranged by size:
1177 314 288
BamHI G'GATC_C
Cuts at: 0 1166 1779
Size: 1166 613
BanI G'GyrC_C
Cuts at: 0 446 1284 1320 1406 1633 1771 1779
Size: 446 838 36 86 227 138 8
Fragments arranged by size:
838 446 227 138 86 36 8
BanII G_rGCy'C
Cuts at: 0 345 1232 1604 1779
Size: 345 887 372 175
Fragments arranged by size:
887 372 345 175
BbsI GAAGACnn'nnnn_
Cuts at: 0 91 1028 1779
Size: 91 937 751
Fragments arranged by size:
937 751 91
BbvCI CC'TCA_GC
Cuts at: 0 1340 1779
Size: 1340 439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 813 847 1481 1515 1779
Size: 813 34 634 34 264
Fragments arranged by size:
813 634 264 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 328 778 1779
Size: 328 450 1001
Fragments arranged by size:
1001 450 328
BclI T'GATC_A
Cuts at: 0 134 1779
Size: 134 1645
BglI GCCn_nnn'nGGC
Cuts at: 0 589 1779
Size: 589 1190
Bme1580I G_kGCm'C
Cuts at: 0 345 911 1178 1289 1676 1779
Size: 345 566 267 111 387 103
Fragments arranged by size:
566 387 345 267 111 103
BmgBI CAC'GTC
Cuts at: 0 89 1248 1779
Size: 89 1159 531
Fragments arranged by size:
1159 531 89
BmrI ACTGGGnnnn_n'
Cuts at: 0 167 606 1779
Size: 167 439 1173
Fragments arranged by size:
1173 439 167
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 254 1365 1779
Size: 254 1111 414
Fragments arranged by size:
1111 414 254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 1542 1779
Size: 832 710 237
Fragments arranged by size:
832 710 237
Bpu10I CC'TnA_GC
Cuts at: 0 363 1340 1779
Size: 363 977 439
Fragments arranged by size:
977 439 363
BsaAI yAC'GTr
Cuts at: 0 1052 1779
Size: 1052 727
BsaHI Gr'CG_yC
Cuts at: 0 323 578 788 1496 1779
Size: 323 255 210 708 283
Fragments arranged by size:
708 323 283 255 210
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 726 874 994 1779
Size: 726 148 120 785
Fragments arranged by size:
785 726 148 120
BseYI C'CCAG_C
Cuts at: 0 624 1676 1779
Size: 624 1052 103
Fragments arranged by size:
1052 624 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 1779
Size: 249 1530
BsiEI CG_ry'CG
Cuts at: 0 254 1211 1215 1491 1779
Size: 254 957 4 276 288
Fragments arranged by size:
957 288 276 254 4
BsiHKAI G_wGCw'C
Cuts at: 0 911 1205 1779
Size: 911 294 574
Fragments arranged by size:
911 574 294
BsmI GAATG_Cn'
Cuts at: 0 283 934 1779
Size: 283 651 845
Fragments arranged by size:
845 651 283
Bsp1286I G_dGCh'C
Cuts at: 0 345 911 1178 1205 1232 1289 1604 1676
Size: 345 566 267 27 27 57 315 72
Cuts at: 1676 1779
Size: 103
Fragments arranged by size:
566 345 315 267 103 72 57 27
27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 664 1779
Size: 664 1115
BsrBI CCG'CTC
Cuts at: 0 261 1474 1779
Size: 261 1213 305
Fragments arranged by size:
1213 305 261
BsrDI GCAATG_nn'
Cuts at: 0 336 1779
Size: 336 1443
BsrFI r'CCGG_y
Cuts at: 0 54 427 646 691 700 1210 1779
Size: 54 373 219 45 9 510 569
Fragments arranged by size:
569 510 373 219 54 45 9
BssSI C'ACGA_G
Cuts at: 0 927 1233 1756 1779
Size: 927 306 523 23
Fragments arranged by size:
927 523 306 23
BstXI CCAn_nnnn'nTGG
Cuts at: 0 810 1762 1779
Size: 810 952 17
Fragments arranged by size:
952 810 17
BstYI r'GATC_y
Cuts at: 0 162 1166 1181 1779
Size: 162 1004 15 598
Fragments arranged by size:
1004 598 162 15
Bsu36I CC'TnA_GG
Cuts at: 0 586 1779
Size: 586 1193
BtgI C'CryG_G
Cuts at: 0 471 501 803 1068 1637 1711 1779
Size: 471 30 302 265 569 74 68
Fragments arranged by size:
569 471 302 265 74 68 30
BtsI GCAGTG_nn'
Cuts at: 0 480 1779
Size: 480 1299
DraI TTT'AAA
Cuts at: 0 117 1034 1779
Size: 117 917 745
Fragments arranged by size:
917 745 117
DraIII CAC_nnn'GTG
Cuts at: 0 1617 1779
Size: 1617 162
EaeI y'GGCC_r
Cuts at: 0 251 648 698 1192 1208 1212 1308 1488
Size: 251 397 50 494 16 4 96 180
Cuts at: 1488 1779
Size: 291
Fragments arranged by size:
494 397 291 251 180 96 50 16
4
EagI C'GGCC_G
Cuts at: 0 251 1208 1212 1488 1779
Size: 251 957 4 276 291
Fragments arranged by size:
957 291 276 251 4
EarI CTCTTCn'nnn_
Cuts at: 0 892 1779
Size: 892 887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 157 1779
Size: 157 1622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 291 331 832 1542 1779
Size: 103 188 40 501 710 237
Fragments arranged by size:
710 501 237 188 103 40
EcoO109I rG'GnC_Cy
Cuts at: 0 342 563 1478 1563 1779
Size: 342 221 915 85 216
Fragments arranged by size:
915 342 221 216 85
FseI GG_CCGG'CC
Cuts at: 0 1214 1779
Size: 1214 565
HaeII r_GCGC'y
Cuts at: 0 625 1779
Size: 625 1154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1650 1682 1779
Size: 1650 32 97
Fragments arranged by size:
1650 97 32
KpnI G_GTAC'C
Cuts at: 0 1324 1779
Size: 1324 455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1625 1779
Size: 1625 154
MscI TGG'CCA
Cuts at: 0 1310 1779
Size: 1310 469
MslI CAynn'nnrTG
Cuts at: 0 419 452 694 869 1376 1760 1779
Size: 419 33 242 175 507 384 19
Fragments arranged by size:
507 419 384 242 175 33 19
MspA1I CmG'CkG
Cuts at: 0 473 1269 1639 1779
Size: 473 796 370 140
Fragments arranged by size:
796 473 370 140
NaeI GCC'GGC
Cuts at: 0 648 702 1212 1779
Size: 648 54 510 567
Fragments arranged by size:
648 567 510 54
NcoI C'CATG_G
Cuts at: 0 803 1068 1779
Size: 803 265 711
Fragments arranged by size:
803 711 265
NgoMIV G'CCGG_C
Cuts at: 0 646 700 1210 1779
Size: 646 54 510 569
Fragments arranged by size:
646 569 510 54
NotI GC'GGCC_GC
Cuts at: 0 251 1779
Size: 251 1528
NspI r_CATG'y
Cuts at: 0 528 907 1779
Size: 528 379 872
Fragments arranged by size:
872 528 379
PflMI CCAn_nnn'nTGG
Cuts at: 0 618 1272 1317 1779
Size: 618 654 45 462
Fragments arranged by size:
654 618 462 45
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 1779
Size: 346 32 1401
Fragments arranged by size:
1401 346 32
PpuMI rG'GwC_Cy
Cuts at: 0 1563 1779
Size: 1563 216
PspOMI G'GGCC_C
Cuts at: 0 341 1779
Size: 341 1438
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1111 1143 1779
Size: 1111 32 636
Fragments arranged by size:
1111 636 32
PstI C_TGCA'G
Cuts at: 0 1334 1721 1779
Size: 1334 387 58
Fragments arranged by size:
1334 387 58
PvuII CAG'CTG
Cuts at: 0 1269 1779
Size: 1269 510
SacII CC_GC'GG
Cuts at: 0 474 1640 1779
Size: 474 1166 139
Fragments arranged by size:
1166 474 139
SanDI GG'GwC_CC
Cuts at: 0 1563 1779
Size: 1563 216
SfcI C'TryA_G
Cuts at: 0 1330 1717 1779
Size: 1330 387 62
Fragments arranged by size:
1330 387 62
SmaI CCC'GGG
Cuts at: 0 1179 1779
Size: 1179 600
SphI G_CATG'C
Cuts at: 0 528 1779
Size: 528 1251
SspI AAT'ATT
Cuts at: 0 204 1779
Size: 204 1575
StyI C'CwwG_G
Cuts at: 0 803 1068 1779
Size: 803 265 711
Fragments arranged by size:
803 711 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 625 1264 1779
Size: 625 639 515
Fragments arranged by size:
639 625 515
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1346 1378 1779
Size: 1346 32 401
Fragments arranged by size:
1346 401 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1239 1273 1417 1779
Size: 1239 34 144 362
Fragments arranged by size:
1239 362 144 34
XmaI C'CCGG_G
Cuts at: 0 1177 1779
Size: 1177 602
ZraI GAC'GTC
Cuts at: 0 789 1779
Size: 789 990
Enzymes that do cut:
AatII Acc65I AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaAI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstXI BstYI Bsu36I BtgI BtsI DraI DraIII
EaeI EagI EarI EciI Eco57MI EcoO109I FseI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NotI NspI PflMI PpiI PpuMI PspOMI PsrI
PstI PvuII SacII SanDI SfcI SmaI SphI SspI
StyI TaqII TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AloI AscI
AseI AsiSI AvrII BaeI BbeI BfrBI BglII BlpI
BmtI BpuEI BsaI BsaBI BsaWI BsaXI BsiWI BsmBI
BspEI BspHI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DrdI EcoICRI EcoNI EcoRI EcoRV
FalI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI NarI NdeI NheI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PshAI PsiI PspXI PvuI
RsrII SacI SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI StuI
SwaI TatI Tth111I XbaI XhoI XmnI