################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BpuAI BsaBI BsaWI BseAI BseRI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI HincII HindII HindIII HinfI HpaI KpnI Ksp632I MaeIII MamI MboII MfeI MflI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI ScaI SexAI SfaNI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Alw44I 138 GTGCAC G^TGCAC AlwI 937 GATCC GGATC(4/5) ApaLI 138 GTGCAC G^TGCAC AscI 465 GGCGCGCC GG^CGCGCC AviII 434 TGCGCA TGC^GCA BalI 161 TGGCCA TGG^CCA BbeI 115 GGCGCC GGCGC^C BbrPI 141 CACGTG CAC^GTG BlpI 714 GCTCAGC GC^T[ACGT]AGC BpmI 472 CTGGAG CTGGAG(16/14) Bpu1102I 714 GCTCAGC GC^T[ACGT]AGC BsaAI 141 CACGTG [CT]AC^GT[GA] BsaI 915 GAGACC GGTCTC(1/5) BsiEI 28 CGGCCG CG[GA][CT]^CG BsmBI 679 GAGACG CGTCTC(1/5) BsrDI 754 CATTGC GCAATG(2/0) BsrI 399 ACTGG ACTGG(1/-1) BstXI 383 CCAACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 714 GCTCAGC GC^T[ACGT]AGC DpnII 937 GATC ^GATC DraIII 322 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 28 CGGCCG C^GGCCG EclXI 28 CGGCCG C^GGCCG Eco52I 28 CGGCCG C^GGCCG FseI 806 GGCCGGCC GGCCGG^CC FspI 434 TGCGCA TGC^GCA HphI 860 TCACC GGTGA(8/7) KasI 115 GGCGCC G^GCGCC MaeII 142 ACGT A^CGT MboI 937 GATC ^GATC MluNI 161 TGGCCA TGG^CCA MscI 161 TGGCCA TGG^CCA NarI 115 GGCGCC GG^CGCC NdeII 937 GATC ^GATC NspI 330 GCATGT [GA]CATG^[CT] PmaCI 141 CACGTG CAC^GTG PmlI 141 CACGTG CAC^GTG Sau3AI 937 GATC ^GATC SbfI 519 CCTGCAGG CCTGCA^GG SfoI 115 GGCGCC GGC^GCC Sse8387I 519 CCTGCAGG CCTGCA^GG StuI 156 AGGCCT AGG^CCT TspRI 398 CACTG CA[GC]TG[ACGT][ACGT]^ XbaI 576 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 292 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG 364 CAGCTGCTG ApaI 6 GGGCCC GGGCC^C 74 GGGCCC AspHI 138 GTGCAC G[AT]GC[AT]^C 685 GTGCTC BfaI 487 CTAG C^TAG 577 CTAG BsgI 319 CTGCAC GTGCAG(16/14) 586 CTGCAC BsiHKAI 138 GTGCAC G[AT]GC[AT]^C 685 GTGCTC BsmAI 679 GAGAC GTCTC(1/5) 915 GAGAC BspMI 302 ACCTGC ACCTGC(4/8) 590 ACCTGC BstAPI 321 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 654 GCACCCGCTGC EcoT14I 61 CCTTGG C^C[AT][AT]GG 604 CCTTGG KspI 479 CCGCGG CCGC^GG 965 CCGCGG MaeI 487 CTAG C^TAG 577 CTAG MslI 141 CACGTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 949 CACGGCTGTG NlaIII 79 CATG CATG^ 331 CATG PpuMI 106 GGGTCCC [GA]G^G[AT]CC[CT] 779 GGGACCT PstI 361 CTGCAG CTGCA^G 520 CTGCAG SacII 479 CCGCGG CCGC^GG 965 CCGCGG SmaI 198 CCCGGG CCC^GGG 972 CCCGGG StyI 61 CCTTGG C^C[AT][AT]GG 604 CCTTGG XmaCI 198 CCCGGG C^CCGGG 972 CCCGGG XmaI 198 CCCGGG C^CCGGG 972 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccII AciI AcyI AluI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIV PvuII Sau96I ScrFI SfcI TseI