(Circular) (Six-base) MAPSORT of: pFN21AE5016.seq Check: 7235 from: 1 to: 5707 pFN21AE5016 5707 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2604 2604 Size: 5707 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3770 5154 5422 1632 Size: 2138 1384 268 1917 Fragments arranged by size: 2138 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 2150 2958 1166 Size: 984 808 3915 Fragments arranged by size: 3915 984 808 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2168 1184 Size: 984 4723 AlwNI CAG_nnn'CTG Cuts at: 2302 2374 3450 5022 2302 Size: 72 1076 1572 2987 Fragments arranged by size: 2987 1572 1076 72 ApaI G_GGCC'C Cuts at: 2015 2083 2015 Size: 68 5639 ApaLI G'TGCA_C Cuts at: 2143 3764 4920 2143 Size: 1621 1156 2930 Fragments arranged by size: 2930 1621 1156 ApoI r'AATT_y Cuts at: 1493 3016 3242 3394 5503 1493 Size: 1523 226 152 2109 1697 Fragments arranged by size: 2109 1697 1523 226 152 AscI GG'CGCG_CC Cuts at: 2471 2471 Size: 5707 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 2107 2135 2203 2807 2977 3032 1870 Size: 73 164 28 68 604 170 55 4545 Fragments arranged by size: 4545 604 170 164 73 68 55 28 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 2270 2407 2658 3028 Size: 325 217 627 333 150 137 251 370 Cuts at: 3028 4483 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 370 333 325 251 217 150 137 BanII G_rGCy'C Cuts at: 1391 2015 2083 2484 2858 2865 2987 3026 1391 Size: 624 68 401 374 7 122 39 4072 Fragments arranged by size: 4072 624 401 374 122 68 39 7 BbeI G_GCGC'C Cuts at: 2124 2124 Size: 5707 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4736 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3898 3932 1903 Size: 34 50 34 1877 34 3678 Fragments arranged by size: 3678 1877 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4318 136 Size: 107 122 71 631 605 2646 1525 Fragments arranged by size: 2646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5702 5702 Size: 5707 BlpI GC'TnA_GC Cuts at: 2720 3124 2720 Size: 404 5303 Bme1580I G_kGCm'C Cuts at: 1790 2015 2083 2147 2202 3768 4924 1790 Size: 225 68 64 55 1566 1156 2573 Fragments arranged by size: 2573 1566 1156 225 68 64 55 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3438 4396 442 Size: 416 53 83 399 24 2021 958 1753 Fragments arranged by size: 2021 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2498 4367 1239 Size: 1259 1869 2579 Fragments arranged by size: 2579 1869 1259 Bpu10I CC'TnA_GC Cuts at: 2210 2381 2210 Size: 171 5536 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3832 4697 4995 3832 Size: 865 298 4544 Fragments arranged by size: 4544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2914 4370 882 Size: 617 1415 1456 2219 Fragments arranged by size: 2219 1456 1415 617 BsaAI yAC'GTr Cuts at: 493 1204 2148 3498 493 Size: 711 944 1350 2702 Fragments arranged by size: 2702 1350 944 711 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2121 2710 2745 3514 Size: 53 83 186 936 588 589 35 769 Cuts at: 3514 3896 4531 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 769 635 589 588 382 186 83 53 35 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5482 Fragments arranged by size: 5482 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5707 BseYI C'CCAG_C Cuts at: 2563 2851 4910 5406 2563 Size: 288 2059 496 2864 Fragments arranged by size: 2864 2059 496 288 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2309 2576 1163 Size: 573 573 267 4294 Fragments arranged by size: 4294 573 573 267 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2036 3157 3918 4946 1540 Size: 275 186 35 1121 761 1028 2301 Fragments arranged by size: 2301 1121 1028 761 275 186 35 BsiHKAI G_wGCw'C Cuts at: 2147 2694 3768 3853 4924 2147 Size: 547 1074 85 1071 2930 Fragments arranged by size: 2930 1074 1071 547 85 BsmI GAATG_Cn' Cuts at: 3218 3311 3218 Size: 93 5614 BsmBI CGTCTCn'nnnn_ Cuts at: 2678 2678 Size: 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2083 2147 2202 2484 2694 2858 Size: 399 225 68 64 55 282 210 164 Cuts at: 2858 2865 2987 3026 3768 3853 4924 1391 Size: 7 122 39 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 282 225 210 164 122 85 68 64 55 39 7 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2316 2604 3062 844 Size: 1472 288 458 3489 Fragments arranged by size: 3489 1472 458 288 BsrBI CCG'CTC Cuts at: 1394 1823 2285 2579 2638 2773 3594 1394 Size: 429 462 294 59 135 821 3507 Fragments arranged by size: 3507 821 462 429 294 135 59 BsrDI GCAATG_nn' Cuts at: 65 1248 2758 4202 4376 65 Size: 1183 1510 1444 174 1396 Fragments arranged by size: 1510 1444 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2456 2463 2655 2812 4351 4538 1700 Size: 110 646 7 192 157 1539 187 2869 Fragments arranged by size: 2869 1539 646 192 187 157 110 7 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5707 BssHII G'CGCG_C Cuts at: 1925 2224 2226 2289 2361 2431 2433 2471 2496 Size: 299 2 63 72 70 2 38 25 Cuts at: 2496 2625 2763 2879 1925 Size: 129 138 116 4753 Fragments arranged by size: 4753 299 138 129 116 72 70 63 38 25 2 2 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 2332 2665 794 Size: 1538 333 3836 Fragments arranged by size: 3836 1538 333 BstBI TT'CG_AA Cuts at: 4511 4511 Size: 5707 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstXI CCAn_nnnn'nTGG Cuts at: 2395 2395 Size: 5707 BstYI r'GATC_y Cuts at: 1972 3037 3404 3789 3806 5258 5344 5356 5702 Size: 1065 367 385 17 1452 86 12 346 Cuts at: 5702 1972 Size: 1977 Fragments arranged by size: 1977 1452 1065 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2094 2484 2793 2953 2970 513 Size: 552 1029 390 309 160 17 3250 Fragments arranged by size: 3250 1029 552 390 309 160 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 3223 4017 4037 3223 Size: 794 20 4893 Fragments arranged by size: 4893 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DraIII CAC_nnn'GTG Cuts at: 2332 2332 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2033 2091 2166 Size: 54 1425 50 275 18 203 58 75 Cuts at: 2166 2454 2702 2790 3154 4043 5672 8 Size: 288 248 88 364 889 1629 43 Fragments arranged by size: 1629 1425 889 364 288 275 248 203 88 75 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2033 3154 1537 Size: 275 221 1121 4090 Fragments arranged by size: 4090 1121 275 221 EarI CTCTTCn'nnn_ Cuts at: 1353 3637 1353 Size: 2284 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2900 4294 4668 4814 387 Size: 1064 138 1311 1394 374 146 1280 Fragments arranged by size: 1394 1311 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2498 3770 4367 5154 5422 1239 Size: 393 866 1272 597 787 268 1524 Fragments arranged by size: 1524 1272 866 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2011 2012 2112 2353 2785 2011 Size: 1 100 241 432 4933 Fragments arranged by size: 4933 432 241 100 1 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 FseI GG_CCGG'CC Cuts at: 2816 2816 Size: 5707 FspI TGC'GCA Cuts at: 2441 4213 2441 Size: 1772 3935 HaeII r_GCGC'y Cuts at: 1678 2124 2302 2388 2492 2852 4854 1678 Size: 446 178 86 104 360 2002 2531 Fragments arranged by size: 2531 2002 446 360 178 104 86 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373 Size: 32 135 32 2776 32 42 32 2626 Fragments arranged by size: 2776 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3051 3297 669 Size: 988 281 1113 246 3079 Fragments arranged by size: 3079 1113 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KasI G'GCGC_C Cuts at: 2120 2120 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2766 4821 5005 1305 Size: 1461 2055 184 2007 Fragments arranged by size: 2055 2007 1461 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2168 10 Size: 54 1425 343 336 3549 Fragments arranged by size: 3549 1425 343 336 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2958 3665 4024 4183 518 Size: 648 984 808 707 359 159 2042 Fragments arranged by size: 2042 984 808 707 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2172 2371 2395 2428 2486 2506 2665 Size: 432 134 199 24 33 58 20 159 Cuts at: 2665 2972 2993 3123 3800 4948 5193 1606 Size: 307 21 130 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 432 307 245 199 159 134 130 58 33 24 21 20 NaeI GCC'GGC Cuts at: 2458 2657 2814 2458 Size: 199 157 5351 Fragments arranged by size: 5351 199 157 NarI GG'CG_CC Cuts at: 2121 2121 Size: 5707 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5155 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NgoMIV G'CCGG_C Cuts at: 2456 2655 2812 2456 Size: 199 157 5351 Fragments arranged by size: 5351 199 157 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 2339 3065 4610 1132 Size: 97 1110 726 1545 2229 Fragments arranged by size: 2229 1545 1110 726 97 PasI CC'CwG_GG Cuts at: 1348 2116 1348 Size: 768 4939 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PmlI CAC'GTG Cuts at: 2148 2148 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2112 2785 2112 Size: 673 5034 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PspOMI G'GGCC_C Cuts at: 2011 2079 2011 Size: 68 5639 PstI C_TGCA'G Cuts at: 830 2370 2529 3059 830 Size: 1540 159 530 3478 Fragments arranged by size: 3478 1540 530 159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 PvuII CAG'CTG Cuts at: 2172 2371 2395 2172 Size: 199 24 5484 Fragments arranged by size: 5484 199 24 SacII CC_GC'GG Cuts at: 2487 2973 2487 Size: 486 5221 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SanDI GG'GwC_CC Cuts at: 2112 2112 Size: 5707 SbfI CC_TGCA'GG Cuts at: 2529 3059 2529 Size: 530 5177 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SfcI C'TryA_G Cuts at: 826 1045 2294 2366 2525 3055 4190 4871 5062 Size: 219 1249 72 159 530 1135 681 191 Cuts at: 5062 826 Size: 1471 Fragments arranged by size: 1471 1249 1135 681 530 219 191 159 72 SfoI GGC'GCC Cuts at: 2122 2122 Size: 5707 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 2205 2979 3034 1872 Size: 333 774 55 4545 Fragments arranged by size: 4545 774 333 55 SmlI C'TyrA_G Cuts at: 820 1017 1943 3811 4712 4974 820 Size: 197 926 1868 901 262 1553 Fragments arranged by size: 1868 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 2163 1517 Size: 646 5061 StyI C'CwwG_G Cuts at: 513 1065 2066 2609 3146 513 Size: 552 1001 543 537 3074 Fragments arranged by size: 3074 1001 552 543 537 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3734 3751 3904 4089 4534 325 Size: 1255 2154 17 153 185 445 1498 Fragments arranged by size: 2154 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3953 5665 96 Size: 274 80 33 51 1443 1976 1712 138 Fragments arranged by size: 1976 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5667 5699 5667 Size: 32 5675 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 2581 3043 2581 Size: 462 5245 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 2203 2977 3032 1870 Size: 333 774 55 4545 Fragments arranged by size: 4545 774 333 55 XmnI GAAnn'nnTTC Cuts at: 3836 3836 Size: 5707 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI XcmI