################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AvaI AviII AvrII BanI BbeI BbrPI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MfeI MluI MroI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tsp45I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 268 GTCGAC GT^[AC][GT]AC AlwNI 430 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 562 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 102 TGGCCA TGG^CCA BamHI 398 GGATCC G^GATCC BanII 352 GGGCTC G[GA]GC[CT]^C BbsI 19 GAAGAC GAAGAC(2/6) BbvCI 594 CCTCAGC CCTCAGC(-5/-2) BglI 473 GCCCCAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 638 AGATCT A^GATCT BpuAI 19 GAAGAC GAAGAC(2/6) BsaBI 393 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 587 GTGCAG GTGCAG(16/14) BsmFI 554 GGGAC GGGAC(10/14) BstAPI 542 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 453 GCGC GCG^C DraII 554 GGGACCT [GA]G^G[ACGT]CC[CT] DrdI 177 GACAGGAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 74 CCACGG C^C[GA][CT]GG EcoO109I 554 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 41 CCAAGG C^C[AT][AT]GG HaeII 452 GGCGCT [GA]GCGC^[CT] HhaI 453 GCGC GCG^C HinP1I 453 GCGC G^CGC HincII 268 GTCGAC GT[CT]^[GA]AC HindII 268 GTCGAC GT[CT]^[GA]AC MamI 393 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 19 GAAGA GAAGA(8/7) MluNI 102 TGGCCA TGG^CCA MscI 102 TGGCCA TGG^CCA MspA1I 244 CCGCTG C[AC]G^C[GT]G NspI 86 GCATGC [GA]CATG^[CT] PflFI 562 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 379 GACTC GAGTC(4/5) PpuMI 554 GGGACCT [GA]G^G[AT]CC[CT] SalI 268 GTCGAC G^TCGAC SphI 86 GCATGC GCATG^C StuI 591 AGGCCT AGG^CCT StyI 41 CCAAGG C^C[AT][AT]GG TfiI 522 GATTC G^A[AT]TC Tsp509I 32 AATT ^AATT Tth111I 562 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 299 CCAAGACCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Asp700I 27 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 336 GAACTACTTC AvaII 555 GGACC G^G[AT]CC 567 GGTCC BmyI 191 GTGCCC G[AGT]GC[ACT]^C 352 GGGCTC BpmI 111 CTCCAG CTGGAG(16/14) 355 CTCCAG BseRI 316 GAGGAG GAGGAG(10/8) 610 GAGGAG Bsp1286I 191 GTGCCC G[AGT]GC[ACT]^C 352 GGGCTC BsrI 56 ACTGG ACTGG(1/-1) 402 CCAGT BstYI 398 GGATCC [GA]^GATC[CT] 638 AGATCT DdeI 595 CTCAG C^T[ACGT]AG 664 CTGAG EcoNI 309 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 603 CCTGGCCGAGG HinfI 379 GACTC G^A[ACGT]TC 522 GATTC MflI 398 GGATCC [GA]^GATC[CT] 638 AGATCT PstI 145 CTGCAG CTGCA^G 582 CTGCAG SfaNI 116 GCATC GCATC(5/9) 482 GCATC TaqI 119 TCGA T^CGA 269 TCGA TspRI 403 CAGTG CA[GC]TG[ACGT][ACGT]^ 585 CAGTG TthHB8I 119 TCGA T^CGA 269 TCGA XhoII 398 GGATCC [GA]^GATC[CT] 638 AGATCT XmnI 27 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 336 GAACTACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI AlwI BbvI BcnI BsaJI BsiYI BslI BssKI BstNI Cac8I Cfr13I DpnII EaeI EcoRII Fnu4HI HaeIII HapII HpaII HphI ItaI MaeII MboI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfcI TseI