(Circular) (Six-base) MAPSORT of: pFN21AE1717.seq Check: 9336 from: 1 to: 5374 pFN21AE1717 5374 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3184 278 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2274 2717 1937 Size: 337 443 4594 Fragments arranged by size: 4594 443 337 Acc65I G'GTAC_C Cuts at: 2695 2695 Size: 5374 AclI AA'CG_TT Cuts at: 1563 3501 3874 1563 Size: 1938 373 3063 Fragments arranged by size: 3063 1938 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3437 4821 5089 1632 Size: 1805 1384 268 1917 Fragments arranged by size: 1917 1805 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5177 AflIII A'CryG_T Cuts at: 1203 4273 5250 1203 Size: 3070 977 1327 Fragments arranged by size: 3070 1327 977 AgeI A'CCGG_T Cuts at: 4205 4205 Size: 5374 AhdI GACnn_n'nnGTC Cuts at: 4103 4103 Size: 5374 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5374 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5374 AlwNI CAG_nnn'CTG Cuts at: 2440 3117 4689 2440 Size: 677 1572 3125 Fragments arranged by size: 3125 1572 677 ApaLI G'TGCA_C Cuts at: 3431 4587 3431 Size: 1156 4218 ApoI r'AATT_y Cuts at: 1493 2683 2909 3061 5170 1493 Size: 1190 226 152 2109 1697 Fragments arranged by size: 2109 1697 1190 226 152 AseI AT'TA_AT Cuts at: 160 1032 3928 160 Size: 872 2896 1606 Fragments arranged by size: 2896 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 AvaI C'yCGr_G Cuts at: 1870 1943 2699 1870 Size: 73 756 4545 Fragments arranged by size: 4545 756 73 BamHI G'GATC_C Cuts at: 2403 2704 3071 2403 Size: 301 367 4706 Fragments arranged by size: 4706 367 301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2695 4150 618 Size: 325 217 627 908 1455 1842 Fragments arranged by size: 1842 1455 908 627 325 217 BanII G_rGCy'C Cuts at: 1391 2361 2693 1391 Size: 970 332 4072 Fragments arranged by size: 4072 970 332 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2031 928 Size: 971 132 4271 Fragments arranged by size: 4271 971 132 BbvCI CC'TCA_GC Cuts at: 2600 2600 Size: 5374 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3565 3599 1903 Size: 34 1628 34 3678 Fragments arranged by size: 3678 1628 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3266 4476 3266 Size: 1210 4164 BclI T'GATC_A Cuts at: 1548 1548 Size: 5374 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2484 3985 136 Size: 107 122 71 631 605 812 1501 1525 Fragments arranged by size: 1525 1501 812 631 605 122 107 71 BglII A'GATC_T Cuts at: 2643 5369 2643 Size: 2726 2648 BlpI GC'TnA_GC Cuts at: 2791 2791 Size: 5374 Bme1580I G_kGCm'C Cuts at: 1790 2200 3435 4591 1790 Size: 410 1235 1156 2573 Fragments arranged by size: 2573 1235 1156 410 BmgBI CAC'GTC Cuts at: 1318 2395 2539 2569 1318 Size: 1077 144 30 4123 Fragments arranged by size: 4123 1077 144 30 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3105 4063 442 Size: 416 53 83 399 24 1688 958 1753 Fragments arranged by size: 1753 1688 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5374 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 5374 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2101 2345 4034 1239 Size: 862 244 1689 2579 Fragments arranged by size: 2579 1689 862 244 Bpu10I CC'TnA_GC Cuts at: 2600 2600 Size: 5374 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3499 4364 4662 3499 Size: 865 298 4211 Fragments arranged by size: 4211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4037 882 Size: 617 2538 2219 Fragments arranged by size: 2538 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3165 493 Size: 711 1961 2702 Fragments arranged by size: 2702 1961 711 BsaBI GATnn'nnATC Cuts at: 2402 5368 2402 Size: 2966 2408 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3181 3563 4198 275 Size: 53 83 186 936 1648 382 635 1451 Fragments arranged by size: 1648 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3807 4205 4479 4626 5037 3807 Size: 398 274 147 411 4144 Fragments arranged by size: 4144 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5149 Fragments arranged by size: 5149 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 2630 1186 Size: 1150 294 3930 Fragments arranged by size: 3930 1150 294 BseYI C'CCAG_C Cuts at: 4577 5073 4577 Size: 496 4878 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2613 1163 Size: 573 877 3924 Fragments arranged by size: 3924 877 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2824 3585 4613 1540 Size: 275 186 823 761 1028 2301 Fragments arranged by size: 2301 1028 823 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3435 3520 4591 3435 Size: 85 1071 4218 Fragments arranged by size: 4218 1071 85 BsmI GAATG_Cn' Cuts at: 2885 2978 2885 Size: 93 5281 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2200 2361 2693 3435 3520 4591 1391 Size: 399 410 161 332 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 410 399 332 161 85 BspEI T'CCGG_A Cuts at: 5037 5037 Size: 5374 BspHI T'CATG_A Cuts at: 3263 4993 3263 Size: 1730 3644 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 844 Size: 1885 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3261 1394 Size: 429 1438 3507 Fragments arranged by size: 3507 1438 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3869 4043 65 Size: 1183 2621 174 1396 Fragments arranged by size: 2621 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4018 4205 1700 Size: 110 2208 187 2869 Fragments arranged by size: 2869 2208 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5374 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5374 BssSI C'ACGA_G Cuts at: 3434 4446 3434 Size: 1012 4362 BstAPI GCAn_nnn'nTGC Cuts at: 794 2553 794 Size: 1759 3615 BstBI TT'CG_AA Cuts at: 4178 4178 Size: 5374 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5374 BstYI r'GATC_y Cuts at: 1972 2403 2643 2704 3071 3456 3473 4925 5011 Size: 431 240 61 367 385 17 1452 86 Cuts at: 5011 5023 5369 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 431 385 367 346 240 86 61 17 12 BtgI C'CryG_G Cuts at: 513 1065 2079 513 Size: 552 1014 3808 Fragments arranged by size: 3808 1014 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4635 Fragments arranged by size: 4635 683 56 BtsI GCAGTG_nn' Cuts at: 2596 2890 3684 3704 2596 Size: 294 794 20 4266 Fragments arranged by size: 4266 794 294 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5339 DraI TTT'AAA Cuts at: 2678 2678 Size: 5374 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2188 4381 809 Size: 507 872 2193 1802 Fragments arranged by size: 2193 1802 872 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2107 2442 2610 Size: 54 1425 50 275 18 277 335 168 Cuts at: 2610 2821 3710 5339 8 Size: 211 889 1629 43 Fragments arranged by size: 1629 1425 889 335 277 275 211 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2821 1537 Size: 275 1009 4090 Fragments arranged by size: 4090 1009 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3304 1353 Size: 1951 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2465 3961 4335 4481 387 Size: 1064 138 876 1496 374 146 1280 Fragments arranged by size: 1496 1280 1064 876 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 2345 3437 4034 4821 5089 1239 Size: 393 469 244 1092 597 787 268 1524 Fragments arranged by size: 1524 1092 787 597 469 393 268 244 EcoNI CCTnn'n_nnAGG Cuts at: 2318 2612 2318 Size: 294 5080 EcoO109I rG'GnC_Cy Cuts at: 2560 2560 Size: 5374 EcoRI G'AATT_C Cuts at: 2683 3061 2683 Size: 378 4996 FspI TGC'GCA Cuts at: 3880 3880 Size: 5374 HaeII r_GCGC'y Cuts at: 1678 2461 4521 1678 Size: 783 2060 2531 Fragments arranged by size: 2531 2060 783 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4015 4047 4089 4121 1373 Size: 32 135 32 2443 32 42 32 2626 Fragments arranged by size: 2626 2443 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2275 2718 2964 669 Size: 988 281 337 443 246 3079 Fragments arranged by size: 3079 988 443 337 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5374 HpaI GTT'AAC Cuts at: 2964 2964 Size: 5374 KpnI G_GTAC'C Cuts at: 2699 2699 Size: 5374 MfeI C'AATT_G Cuts at: 2973 2973 Size: 5374 MluI A'CGCG_T Cuts at: 5250 5250 Size: 5374 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4488 4672 1305 Size: 3183 184 2007 Fragments arranged by size: 3183 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2109 10 Size: 54 1425 343 277 3275 Fragments arranged by size: 3275 1425 343 277 54 MslI CAynn'nnrTG Cuts at: 518 1166 3332 3691 3850 518 Size: 648 2166 359 159 2042 Fragments arranged by size: 2166 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2251 2790 3467 4615 4860 1606 Size: 645 539 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 645 539 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4822 NdeI CA'TA_TG Cuts at: 387 387 Size: 5374 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5374 NotI GC'GGCC_GC Cuts at: 2821 2821 Size: 5374 NspI r_CATG'y Cuts at: 1132 1229 2095 2732 4277 1132 Size: 97 866 637 1545 2229 Fragments arranged by size: 2229 1545 866 637 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5374 PciI A'CATG_T Cuts at: 4273 4273 Size: 5374 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5374 PfoI T'CCnGG_A Cuts at: 2138 2361 2138 Size: 223 5151 PmeI GTTT'AAAC Cuts at: 2678 2678 Size: 5374 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3443 3475 4956 4988 3443 Size: 32 1481 32 3829 Fragments arranged by size: 3829 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2560 2560 Size: 5374 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5374 PsiI TTA'TAA Cuts at: 2944 2944 Size: 5374 PstI C_TGCA'G Cuts at: 830 2154 2591 2726 830 Size: 1324 437 135 3478 Fragments arranged by size: 3478 1324 437 135 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5374 SalI G'TCGA_C Cuts at: 1936 2273 2716 1936 Size: 337 443 4594 Fragments arranged by size: 4594 443 337 SbfI CC_TGCA'GG Cuts at: 2726 2726 Size: 5374 ScaI AGT'ACT Cuts at: 3622 5334 3622 Size: 1712 3662 SfcI C'TryA_G Cuts at: 826 1045 2150 2587 2656 2722 3857 4538 4729 Size: 219 1105 437 69 66 1135 681 191 Cuts at: 4729 826 Size: 1471 Fragments arranged by size: 1471 1135 1105 681 437 219 191 69 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5374 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5374 SmaI CCC'GGG Cuts at: 1872 2701 1872 Size: 829 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3478 4379 4641 820 Size: 197 926 1535 901 262 1553 Fragments arranged by size: 1553 1535 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3165 493 Size: 2672 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5374 SphI G_CATG'C Cuts at: 1132 2095 2732 1132 Size: 963 637 3774 Fragments arranged by size: 3774 963 637 SspI AAT'ATT Cuts at: 5 52 3298 5 Size: 47 3246 2081 Fragments arranged by size: 3246 2081 47 StuI AGG'CCT Cuts at: 1517 2598 1517 Size: 1081 4293 StyI C'CwwG_G Cuts at: 513 1065 2046 2813 513 Size: 552 981 767 3074 Fragments arranged by size: 3074 981 767 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2092 2401 3401 3418 3571 3756 4201 Size: 1255 512 309 1000 17 153 185 445 Cuts at: 4201 325 Size: 1498 Fragments arranged by size: 1498 1255 1000 512 445 309 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3620 5332 96 Size: 274 80 33 51 1443 1643 1712 138 Fragments arranged by size: 1712 1643 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5334 5366 5334 Size: 32 5342 Tth111I GACn'n_nGTC Cuts at: 1316 2570 1316 Size: 1254 4120 XbaI T'CTAG_A Cuts at: 2710 2710 Size: 5374 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2311 2311 Size: 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5374 XmaI C'CCGG_G Cuts at: 1870 2699 1870 Size: 829 4545 XmnI GAAnn'nnTTC Cuts at: 2036 2345 3503 2036 Size: 309 1158 3907 Fragments arranged by size: 3907 1158 309 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3182 276 Size: 53 83 186 936 1648 2468 Fragments arranged by size: 2468 1648 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI