(Linear) (Six-base) MAPSORT of: KIEE6064.seq Check: 4171 from: 1 to: 960 KIEE6064.orf With 182 enzymes: SgfI * December 5, 2014 13:21 .. AccI GT'mk_AC Cuts at: 0 113 960 Size: 113 847 AgeI A'CCGG_T Cuts at: 0 730 960 Size: 730 230 AleI CACnn'nnGTG Cuts at: 0 751 960 Size: 751 209 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 166 960 Size: 166 794 ApoI r'AATT_y Cuts at: 0 43 960 Size: 43 917 AvaI C'yCGr_G Cuts at: 0 20 275 960 Size: 20 255 685 Fragments arranged by size: 685 255 20 Bme1580I G_kGCm'C Cuts at: 0 306 960 Size: 306 654 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 292 786 945 960 Size: 292 494 159 15 Fragments arranged by size: 494 292 159 15 Bpu10I CC'TnA_GC Cuts at: 0 508 960 Size: 508 452 BsaAI yAC'GTr Cuts at: 0 381 960 Size: 381 579 BsaWI w'CCGG_w Cuts at: 0 730 960 Size: 730 230 BseYI C'CCAG_C Cuts at: 0 71 129 444 676 796 960 Size: 71 58 315 232 120 164 Fragments arranged by size: 315 232 164 120 71 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 597 960 Size: 597 363 BsiEI CG_ry'CG Cuts at: 0 704 960 Size: 704 256 BsmBI CGTCTCn'nnnn_ Cuts at: 0 866 960 Size: 866 94 Bsp1286I G_dGCh'C Cuts at: 0 306 960 Size: 306 654 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 86 109 272 316 797 960 Size: 86 23 163 44 481 163 Fragments arranged by size: 481 163 163 86 44 23 BsrBI CCG'CTC Cuts at: 0 521 960 Size: 521 439 BsrFI r'CCGG_y Cuts at: 0 730 960 Size: 730 230 BssSI C'ACGA_G Cuts at: 0 573 960 Size: 573 387 BstAPI GCAn_nnn'nTGC Cuts at: 0 475 960 Size: 475 485 Bsu36I CC'TnA_GG Cuts at: 0 913 960 Size: 913 47 BtgI C'CryG_G Cuts at: 0 26 362 638 752 960 Size: 26 336 276 114 208 Fragments arranged by size: 336 276 208 114 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 657 960 Size: 657 303 BtsI GCAGTG_nn' Cuts at: 0 476 559 585 960 Size: 476 83 26 375 Fragments arranged by size: 476 375 83 26 EaeI y'GGCC_r Cuts at: 0 200 383 566 755 960 Size: 200 183 183 189 205 Fragments arranged by size: 205 200 189 183 183 EarI CTCTTCn'nnn_ Cuts at: 0 97 613 960 Size: 97 516 347 Fragments arranged by size: 516 347 97 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 225 493 960 Size: 225 268 467 Fragments arranged by size: 467 268 225 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 292 786 945 960 Size: 292 494 159 15 Fragments arranged by size: 494 292 159 15 EcoO109I rG'GnC_Cy Cuts at: 0 75 909 953 960 Size: 75 834 44 7 Fragments arranged by size: 834 75 44 7 EcoRI G'AATT_C Cuts at: 0 43 960 Size: 43 917 EcoRV GAT'ATC Cuts at: 0 552 960 Size: 552 408 HaeII r_GCGC'y Cuts at: 0 151 960 Size: 151 809 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 269 301 960 Size: 269 32 659 Fragments arranged by size: 659 269 32 MscI TGG'CCA Cuts at: 0 202 385 568 757 960 Size: 202 183 183 189 203 Fragments arranged by size: 203 202 189 183 183 MslI CAynn'nnrTG Cuts at: 0 142 184 751 781 960 Size: 142 42 567 30 179 Fragments arranged by size: 567 179 142 42 30 MspA1I CmG'CkG Cuts at: 0 28 960 Size: 28 932 NcoI C'CATG_G Cuts at: 0 362 960 Size: 362 598 NspI r_CATG'y Cuts at: 0 696 960 Size: 696 264 PfoI T'CCnGG_A Cuts at: 0 523 960 Size: 523 437 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 590 622 960 Size: 590 32 338 Fragments arranged by size: 590 338 32 PpuMI rG'GwC_Cy Cuts at: 0 75 953 960 Size: 75 878 7 Fragments arranged by size: 878 75 7 PshAI GACnn'nnGTC Cuts at: 0 587 960 Size: 587 373 SacII CC_GC'GG Cuts at: 0 29 960 Size: 29 931 SanDI GG'GwC_CC Cuts at: 0 953 960 Size: 953 7 SexAI A'CCwGG_T Cuts at: 0 116 960 Size: 116 844 SfcI C'TryA_G Cuts at: 0 879 960 Size: 879 81 SgrAI Cr'CCGG_yG Cuts at: 0 730 960 Size: 730 230 SmaI CCC'GGG Cuts at: 0 22 960 Size: 22 938 StuI AGG'CCT Cuts at: 0 458 960 Size: 458 502 StyI C'CwwG_G Cuts at: 0 362 960 Size: 362 598 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 451 690 960 Size: 451 239 270 Fragments arranged by size: 451 270 239 TatI w'GTAC_w Cuts at: 0 188 960 Size: 188 772 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 255 810 960 Size: 255 555 150 Fragments arranged by size: 555 255 150 XmaI C'CCGG_G Cuts at: 0 20 960 Size: 20 940 XmnI GAAnn'nnTTC Cuts at: 0 602 960 Size: 602 358 Enzymes that do cut: AccI AgeI AleI AlfI ApoI AvaI Bme1580I BpmI Bpu10I BsaAI BsaWI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I MscI MslI MspA1I NcoI NspI PfoI PpiI PpuMI PshAI SacII SanDI SexAI SfcI SgrAI SmaI StuI StyI TaqII TatI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AhdI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaXI BseRI BsiHKAI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SgfI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI ZraI