(Linear) (Six-base) MAPSORT of: KIEE2643.seq  Check: 7896  from: 1  to: 315



KIEE2643.orf

 With 182 enzymes: SgfI * 

                        October 21, 2016 17:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      43     315 
   Size:         43     272
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     315 
   Size:        182     133
 
ApoI r'AATT_y

Cuts at:      0     262     315 
   Size:        262      53
 
AseI AT'TA_AT

Cuts at:      0     302     315 
   Size:        302      13
 
BclI T'GATC_A

Cuts at:      0     110     315 
   Size:        110     205
 
BfrBI ATG'CAT

Cuts at:      0     218     315 
   Size:        218      97
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     315 
   Size:        290      25
 
BsaAI yAC'GTr

Cuts at:      0      89     315 
   Size:         89     226
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      43     315 
   Size:         43     272
 
DraIII CAC_nnn'GTG

Cuts at:      0      92     315 
   Size:         92     223
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     290     315 
   Size:        182     108      25

  Fragments arranged by size: 

                 182     108      25
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     138     315 
   Size:        138     177
 
HincII GTy'rAC

Cuts at:      0      75     315 
   Size:         75     240
 
HindIII A'AGCT_T

Cuts at:      0     286     315 
   Size:        286      29
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     315 
   Size:        173     142
 
NsiI A_TGCA'T

Cuts at:      0     220     315 
   Size:        220      95
 
NspI r_CATG'y

Cuts at:      0     222     315 
   Size:        222      93
 
PmlI CAC'GTG

Cuts at:      0      89     315 
   Size:         89     226
 
PstI C_TGCA'G

Cuts at:      0      54     315 
   Size:         54     261
 
SfcI C'TryA_G

Cuts at:      0      50     315 
   Size:         50     265
 
SphI G_CATG'C

Cuts at:      0     222     315 
   Size:        222      93
 
StyI C'CwwG_G

Cuts at:      0      41     315 
   Size:         41     274
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     247     315 
   Size:        247      68
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     239     271     315 
   Size:        239      32      44

  Fragments arranged by size: 

                 239      44      32
 

 Enzymes that do cut:

     AarI     AcuI     ApoI     AseI     BclI    BfrBI     BpmI    BsaAI
    BspMI   DraIII  Eco57MI     FalI   HincII  HindIII     MmeI     NsiI
     NspI     PmlI     PstI     SfcI     SphI     StyI    TaqII     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI