################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssHII Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MluI MroI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AclI 326 AACGTT AA^CGTT BsmBI 1024 CGTCTC CGTCTC(1/5) BsmI 984 GAATGC GAATGC(1/-1) BspLU11I 2624 ACATGT A^CATGT BspMI 818 ACCTGC ACCTGC(4/8) BsrBI 923 GAGCGG CCGCTC(-3/-3) BsrDI 2509 GCAATG GCAATG(2/0) BssSI 785 CACGAG CACGAG(-5/-1) BstAPI 2250 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 842 TTCGAA TT^CGAA DsaI 2156 CCATGG C^C[GA][CT]GG EcoNI 672 CCTCGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 897 GAATTC G^AATTC EcoT14I 2156 CCATGG C^C[AT][AT]GG NcoI 2156 CCATGG C^CATGG NheI 2151 GCTAGC G^CTAGC NspV 842 TTCGAA TT^CGAA Psp1406I 326 AACGTT AA^CGTT SacI 598 GAGCTC GAGCT^C ScaI 1573 AGTACT AGT^ACT SfuI 842 TTCGAA TT^CGAA SphI 1148 GCATGC GCATG^C StuI 2210 AGGCCT AGG^CCT StyI 2156 CCATGG C^C[AT][AT]GG TaqI 843 TCGA T^CGA TthHB8I 843 TCGA T^CGA XbaI 2659 TCTAGA T^CTAGA XcmI 497 CCAGTGAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflIII 528 ACGTGT A^C[GA][CT]GT 2624 ACATGT Asp700I 663 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1309 GAAGTAATTC AspHI 220 GAGCAC G[AT]GC[AT]^C 598 GAGCTC BalI 485 TGGCCA TGG^CCA 2159 TGGCCA BanII 598 GAGCTC G[GA]GC[CT]^C 2642 GAGCCC BcnI 608 CCCGG CC^[GC]GG 2112 CCGGG BglII 708 AGATCT A^GATCT 2025 AGATCT BsaBI 409 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2902 GATTTTCATC BsaI 353 GAGACC GGTCTC(1/5) 2680 GAGACC BsgI 652 GTGCAG GTGCAG(16/14) 1106 GTGCAG BsiHKAI 220 GAGCAC G[AT]GC[AT]^C 598 GAGCTC CfoI 1428 GCGC GCG^C 2862 GCGC EaeI 485 TGGCCA [CT]^GGCC[GA] 2159 TGGCCA HapII 609 CCGG C^CGG 2112 CCGG HhaI 1428 GCGC GCG^C 2862 GCGC HinP1I 1428 GCGC G^CGC 2862 GCGC HincII 277 GTCAAC GT[CT]^[GA]AC 2364 GTTGAC HindII 277 GTCAAC GT[CT]^[GA]AC 2364 GTTGAC HpaII 609 CCGG C^CGG 2112 CCGG HphI 894 GGTGA GGTGA(8/7) 1461 TCACC MaeII 327 ACGT A^CGT 528 ACGT MamI 409 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2902 GATTTTCATC MfeI 1882 CAATTG C^AATTG 2254 CAATTG MluNI 485 TGGCCA TGG^CCA 2159 TGGCCA MscI 485 TGGCCA TGG^CCA 2159 TGGCCA MspI 609 CCGG C^CGG 2112 CCGG MunI 1882 CAATTG C^AATTG 2254 CAATTG NciI 608 CCCGG CC^[GC]GG 2112 CCGGG SexAI 1253 ACCAGGT A^CC[AT]GGT 2357 ACCAGGT XmnI 663 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1309 GAAGTAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BanI BbsI BbvCI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII