(Circular) (Six-base) MAPSORT of: pFN21AE3338.seq Check: 2862 from: 1 to: 7630 pFN21AE3338 7630 bp With 182 enzymes: SgfI * March 28, 2012 12:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5440 278 Size: 53 83 186 936 3904 2468 Fragments arranged by size: 3904 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4973 1937 Size: 3036 4594 Acc65I G'GTAC_C Cuts at: 4951 4951 Size: 7630 AclI AA'CG_TT Cuts at: 1563 2332 5757 6130 1563 Size: 769 3425 373 3063 Fragments arranged by size: 3425 3063 769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2705 3113 3333 4397 5693 7077 7345 1632 Size: 1073 408 220 1064 1296 1384 268 1917 Fragments arranged by size: 1917 1384 1296 1073 1064 408 268 220 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7433 AflIII A'CryG_T Cuts at: 1203 2533 4629 6529 7506 1203 Size: 1330 2096 1900 977 1327 Fragments arranged by size: 2096 1900 1330 1327 977 AgeI A'CCGG_T Cuts at: 6461 6461 Size: 7630 AhdI GACnn_n'nnGTC Cuts at: 6359 6359 Size: 7630 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7630 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2069 3408 1184 Size: 885 1339 5406 Fragments arranged by size: 5406 1339 885 AlwNI CAG_nnn'CTG Cuts at: 2411 2665 3623 5373 6945 2411 Size: 254 958 1750 1572 3096 Fragments arranged by size: 3096 1750 1572 958 254 ApaLI G'TGCA_C Cuts at: 5687 6843 5687 Size: 1156 6474 ApoI r'AATT_y Cuts at: 1493 2844 2902 3377 4282 4939 5165 5317 7426 Size: 1351 58 475 905 657 226 152 2109 Cuts at: 7426 1493 Size: 1697 Fragments arranged by size: 2109 1697 1351 905 657 475 226 152 58 AseI AT'TA_AT Cuts at: 160 1032 6184 160 Size: 872 5152 1606 Fragments arranged by size: 5152 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7630 AvaI C'yCGr_G Cuts at: 1870 1943 4955 1870 Size: 73 3012 4545 Fragments arranged by size: 4545 3012 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2281 2302 2314 2335 2281 Size: 21 12 21 7576 Fragments arranged by size: 7576 21 21 12 BamHI G'GATC_C Cuts at: 4960 5327 4960 Size: 367 7263 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 3236 4898 4951 6406 Size: 325 217 627 258 1191 1662 53 1455 Cuts at: 6406 618 Size: 1842 Fragments arranged by size: 1842 1662 1455 1191 627 325 258 217 53 BanII G_rGCy'C Cuts at: 1391 2607 4651 4949 1391 Size: 1216 2044 298 4072 Fragments arranged by size: 4072 2044 1216 298 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2535 3284 4230 4385 4816 4850 928 Size: 971 636 749 946 155 431 34 3708 Fragments arranged by size: 3708 971 946 749 636 431 155 34 BbvCI CC'TCA_GC Cuts at: 3305 3368 3646 3305 Size: 63 278 7289 Fragments arranged by size: 7289 278 63 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5821 5855 1903 Size: 34 3884 34 3678 Fragments arranged by size: 3884 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5522 6732 5522 Size: 1210 6420 BclI T'GATC_A Cuts at: 1548 1548 Size: 7630 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6241 136 Size: 107 122 71 631 605 4569 1525 Fragments arranged by size: 4569 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2713 4030 7625 2713 Size: 1317 3595 2718 Fragments arranged by size: 3595 2718 1317 BlpI GC'TnA_GC Cuts at: 5047 5047 Size: 7630 Bme1580I G_kGCm'C Cuts at: 1790 3559 5691 6847 1790 Size: 1769 2132 1156 2573 Fragments arranged by size: 2573 2132 1769 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7630 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3511 3565 3634 Size: 416 53 83 399 24 2094 54 69 Cuts at: 3634 5361 6319 442 Size: 1727 958 1753 Fragments arranged by size: 2094 1753 1727 958 416 399 83 69 54 53 24 BmtI G_CTAG'C Cuts at: 1056 4160 1056 Size: 3104 4526 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2381 2686 4678 2381 Size: 305 1992 5333 Fragments arranged by size: 5333 1992 305 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 2314 2363 2825 3062 3123 3437 4577 Size: 816 259 49 462 237 61 314 1140 Cuts at: 4577 4703 6290 1239 Size: 126 1587 2579 Fragments arranged by size: 2579 1587 1140 816 462 314 259 237 126 61 49 Bpu10I CC'TnA_GC Cuts at: 3305 3368 3646 3825 3305 Size: 63 278 179 7110 Fragments arranged by size: 7110 278 179 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3020 3887 4031 4126 5755 6620 6918 3020 Size: 867 144 95 1629 865 298 3732 Fragments arranged by size: 3732 1629 867 865 298 144 95 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2352 4679 6293 882 Size: 617 853 2327 1614 2219 Fragments arranged by size: 2327 2219 1614 853 617 BsaAI yAC'GTr Cuts at: 493 1204 5421 493 Size: 711 4217 2702 Fragments arranged by size: 4217 2702 711 BsaBI GATnn'nnATC Cuts at: 2418 4911 7624 2418 Size: 2493 2713 2424 Fragments arranged by size: 2713 2493 2424 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5437 5819 6454 275 Size: 53 83 186 936 3904 382 635 1451 Fragments arranged by size: 3904 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6063 6461 6735 6882 7293 6063 Size: 398 274 147 411 6400 Fragments arranged by size: 6400 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3620 3650 4421 4451 1178 Size: 30 165 30 2217 30 771 30 4357 Fragments arranged by size: 4357 2217 771 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3017 3080 4142 4178 4364 1186 Size: 1831 63 1062 36 186 4452 Fragments arranged by size: 4452 1831 1062 186 63 36 BseYI C'CCAG_C Cuts at: 4395 4471 4527 6833 7329 4395 Size: 76 56 2306 496 4696 Fragments arranged by size: 4696 2306 496 76 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2678 3132 1163 Size: 573 942 454 5661 Fragments arranged by size: 5661 942 573 454 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5080 5841 6869 1540 Size: 275 186 3079 761 1028 2301 Fragments arranged by size: 3079 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2229 2607 5691 5776 6847 2229 Size: 378 3084 85 1071 3012 Fragments arranged by size: 3084 3012 1071 378 85 BsmI GAATG_Cn' Cuts at: 2995 5141 5234 2995 Size: 2146 93 5391 Fragments arranged by size: 5391 2146 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3035 3035 Size: 7630 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2607 3559 4651 4949 5691 5776 Size: 399 439 378 952 1092 298 742 85 Cuts at: 5776 6847 1391 Size: 1071 2174 Fragments arranged by size: 2174 1092 1071 952 742 439 399 378 298 85 BspEI T'CCGG_A Cuts at: 7293 7293 Size: 7630 BspHI T'CATG_A Cuts at: 5519 7249 5519 Size: 1730 5900 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2832 4985 844 Size: 1988 2153 3489 Fragments arranged by size: 3489 2153 1988 BsrBI CCG'CTC Cuts at: 1394 1823 2930 5517 1394 Size: 429 1107 2587 3507 Fragments arranged by size: 3507 2587 1107 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4521 6125 6299 65 Size: 1183 3273 1604 174 1396 Fragments arranged by size: 3273 1604 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6274 6461 1700 Size: 110 4464 187 2869 Fragments arranged by size: 4464 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7630 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7630 BssSI C'ACGA_G Cuts at: 2790 5690 6702 2790 Size: 2900 1012 3718 Fragments arranged by size: 3718 2900 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4261 794 Size: 3467 4163 BstBI TT'CG_AA Cuts at: 2848 6434 2848 Size: 3586 4044 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7630 BstYI r'GATC_y Cuts at: 1972 2713 4030 4051 4113 4320 4960 5327 5712 Size: 741 1317 21 62 207 640 367 385 Cuts at: 5712 5729 7181 7267 7279 7625 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1317 741 640 385 367 346 207 86 62 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 4161 513 Size: 552 3096 3982 Fragments arranged by size: 3982 3096 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2717 4898 487 Size: 683 56 1491 2181 3219 Fragments arranged by size: 3219 2181 1491 683 56 BtsI GCAGTG_nn' Cuts at: 2153 2661 2834 3120 4128 5146 5940 5960 2153 Size: 508 173 286 1008 1018 794 20 3823 Fragments arranged by size: 3823 1018 1008 794 508 286 173 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7630 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7595 DraI TTT'AAA Cuts at: 4934 4934 Size: 7630 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6637 809 Size: 507 5321 1802 Fragments arranged by size: 5321 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2490 4164 5077 Size: 54 1425 50 275 18 660 1674 913 Cuts at: 5077 5966 7595 8 Size: 889 1629 43 Fragments arranged by size: 1674 1629 1425 913 889 660 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5077 1537 Size: 275 3265 4090 Fragments arranged by size: 4090 3265 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2131 2502 4335 4416 4731 5560 1353 Size: 778 371 1833 81 315 829 3423 Fragments arranged by size: 3423 1833 829 778 371 315 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6217 6591 6737 387 Size: 1064 138 4628 374 146 1280 Fragments arranged by size: 4628 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2605 2605 Size: 7630 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2314 2363 2705 2825 3062 3113 Size: 393 423 259 49 342 120 237 51 Cuts at: 3113 3123 3333 3437 4397 4577 4703 5693 6290 Size: 10 210 104 960 180 126 990 597 Cuts at: 6290 7077 7345 1239 Size: 787 268 1524 Fragments arranged by size: 1524 990 960 787 597 423 393 342 268 259 237 210 180 126 120 104 51 49 10 EcoNI CCTnn'n_nnAGG Cuts at: 2681 2681 Size: 7630 EcoO109I rG'GnC_Cy Cuts at: 3413 3484 3586 4482 3413 Size: 71 102 896 6561 Fragments arranged by size: 6561 896 102 71 EcoRI G'AATT_C Cuts at: 2902 4939 5317 2902 Size: 2037 378 5215 Fragments arranged by size: 5215 2037 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2234 4777 2234 Size: 2543 5087 FspI TGC'GCA Cuts at: 6136 6136 Size: 7630 HaeII r_GCGC'y Cuts at: 1678 6777 1678 Size: 5099 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2284 2501 2503 2533 Size: 32 135 32 680 32 217 2 30 Cuts at: 2533 2535 2594 2626 2784 2816 3617 3620 3649 Size: 2 59 32 158 32 801 3 29 Cuts at: 3649 3652 3995 4027 6271 6303 6345 6377 1373 Size: 3 343 32 2244 32 42 32 2626 Fragments arranged by size: 2626 2244 801 680 343 217 158 135 59 42 32 32 32 32 32 32 32 32 30 29 3 3 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2284 4371 4974 5220 669 Size: 988 281 346 2087 603 246 3079 Fragments arranged by size: 3079 2087 988 603 346 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7630 HpaI GTT'AAC Cuts at: 5220 5220 Size: 7630 KpnI G_GTAC'C Cuts at: 4955 4955 Size: 7630 MfeI C'AATT_G Cuts at: 3887 4259 5229 3887 Size: 372 970 6288 Fragments arranged by size: 6288 970 372 MluI A'CGCG_T Cuts at: 7506 7506 Size: 7630 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2169 4005 6744 6928 1305 Size: 864 1836 2739 184 2007 Fragments arranged by size: 2739 2007 1836 864 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2492 4166 10 Size: 54 1425 343 660 1674 3474 Fragments arranged by size: 3474 1674 1425 660 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2051 3210 3852 5588 5947 6106 518 Size: 648 885 1159 642 1736 359 159 2042 Fragments arranged by size: 2042 1736 1159 885 648 642 359 159 MspA1I CmG'CkG Cuts at: 1606 2033 2075 2197 2524 3472 3991 4001 5046 Size: 427 42 122 327 948 519 10 1045 Cuts at: 5046 5723 6871 7116 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1045 948 677 519 427 327 245 122 42 10 NcoI C'CATG_G Cuts at: 513 1065 4161 513 Size: 552 3096 3982 Fragments arranged by size: 3982 3096 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7630 NheI G'CTAG_C Cuts at: 1052 4156 1052 Size: 3104 4526 NotI GC'GGCC_GC Cuts at: 5077 5077 Size: 7630 NspI r_CATG'y Cuts at: 1132 1229 3157 4231 4633 4786 4988 6533 1132 Size: 97 1928 1074 402 153 202 1545 2229 Fragments arranged by size: 2229 1928 1545 1074 402 202 153 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7630 PciI A'CATG_T Cuts at: 4629 6529 4629 Size: 1900 5730 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7630 PfoI T'CCnGG_A Cuts at: 2612 2802 2612 Size: 190 7440 PmeI GTTT'AAAC Cuts at: 4934 4934 Size: 7630 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2850 2882 3969 4001 5699 5731 7212 7244 2850 Size: 32 1087 32 1698 32 1481 32 3236 Fragments arranged by size: 3236 1698 1481 1087 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3484 3586 4482 3484 Size: 102 896 6632 Fragments arranged by size: 6632 896 102 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7630 PsiI TTA'TAA Cuts at: 2916 5200 2916 Size: 2284 5346 PstI C_TGCA'G Cuts at: 830 2529 2829 3567 4982 830 Size: 1699 300 738 1415 3478 Fragments arranged by size: 3478 1699 1415 738 300 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7630 PvuII CAG'CTG Cuts at: 2033 2075 2524 3472 3991 4001 2033 Size: 42 449 948 519 10 5662 Fragments arranged by size: 5662 948 519 449 42 10 SacI G_AGCT'C Cuts at: 2607 2607 Size: 7630 SalI G'TCGA_C Cuts at: 1936 4972 1936 Size: 3036 4594 SbfI CC_TGCA'GG Cuts at: 4982 4982 Size: 7630 ScaI AGT'ACT Cuts at: 3580 5878 7590 3580 Size: 2298 1712 3620 Fragments arranged by size: 3620 2298 1712 SexAI A'CCwGG_T Cuts at: 3258 4362 3258 Size: 1104 6526 SfcI C'TryA_G Cuts at: 826 1045 2525 2699 2825 3191 3563 4145 4400 Size: 219 1480 174 126 366 372 582 255 Cuts at: 4400 4978 6113 6794 6985 826 Size: 578 1135 681 191 1471 Fragments arranged by size: 1480 1471 1135 681 582 578 372 366 255 219 191 174 126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7630 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7630 SmaI CCC'GGG Cuts at: 1872 4957 1872 Size: 3085 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2999 3902 4046 4105 5734 6635 Size: 197 926 1056 903 144 59 1629 901 Cuts at: 6635 6897 820 Size: 262 1553 Fragments arranged by size: 1629 1553 1056 926 903 901 262 197 144 59 SnaBI TAC'GTA Cuts at: 493 5421 493 Size: 4928 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7630 SphI G_CATG'C Cuts at: 1132 3157 4988 1132 Size: 2025 1831 3774 Fragments arranged by size: 3774 2025 1831 SspI AAT'ATT Cuts at: 5 52 5554 5 Size: 47 5502 2081 Fragments arranged by size: 5502 2081 47 StuI AGG'CCT Cuts at: 1517 4217 1517 Size: 2700 4930 StyI C'CwwG_G Cuts at: 513 1065 4161 5069 513 Size: 552 3096 908 3074 Fragments arranged by size: 3096 3074 908 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2454 5657 5674 5827 6012 6457 325 Size: 1255 874 3203 17 153 185 445 1498 Fragments arranged by size: 3203 1498 1255 874 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3578 5876 7588 Size: 274 80 33 51 1443 1601 2298 1712 Cuts at: 7588 96 Size: 138 Fragments arranged by size: 2298 1712 1601 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7590 7622 7590 Size: 32 7598 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7630 XbaI T'CTAG_A Cuts at: 4664 4966 4664 Size: 302 7328 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2509 2509 Size: 7630 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7630 XmaI C'CCGG_G Cuts at: 1870 4955 1870 Size: 3085 4545 XmnI GAAnn'nnTTC Cuts at: 2672 3318 5759 2672 Size: 646 2441 4543 Fragments arranged by size: 4543 2441 646 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5438 276 Size: 53 83 186 936 3904 2468 Fragments arranged by size: 3904 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI