(Linear) (Six-base) MAPSORT of: KIEE3338.seq Check: 4841 from: 1 to: 2925 KIEE3338.orf With 182 enzymes: SgfI * March 22, 2012 16:30 .. AclI AA'CG_TT Cuts at: 0 327 2925 Size: 327 2598 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 700 1108 1328 2392 2925 Size: 700 408 220 1064 533 Fragments arranged by size: 1064 700 533 408 220 AflIII A'CryG_T Cuts at: 0 528 2624 2925 Size: 528 2096 301 Fragments arranged by size: 2096 528 301 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 64 1403 2925 Size: 64 1339 1522 Fragments arranged by size: 1522 1339 64 AlwNI CAG_nnn'CTG Cuts at: 0 406 660 1618 2925 Size: 406 254 958 1307 Fragments arranged by size: 1307 958 406 254 ApoI r'AATT_y Cuts at: 0 839 897 1372 2277 2925 Size: 839 58 475 905 648 Fragments arranged by size: 905 839 648 475 58 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 276 297 309 330 2925 Size: 276 21 12 21 2595 Fragments arranged by size: 2595 276 21 21 12 BanI G'GyrC_C Cuts at: 0 40 1231 2893 2925 Size: 40 1191 1662 32 Fragments arranged by size: 1662 1191 40 32 BanII G_rGCy'C Cuts at: 0 602 2646 2925 Size: 602 2044 279 Fragments arranged by size: 2044 602 279 BbsI GAAGACnn'nnnn_ Cuts at: 0 530 1279 2225 2380 2811 2845 2925 Size: 530 749 946 155 431 34 80 Fragments arranged by size: 946 749 530 431 155 80 34 BbvCI CC'TCA_GC Cuts at: 0 1300 1363 1641 2925 Size: 1300 63 278 1284 Fragments arranged by size: 1300 1284 278 63 BglII A'GATC_T Cuts at: 0 708 2025 2925 Size: 708 1317 900 Fragments arranged by size: 1317 900 708 Bme1580I G_kGCm'C Cuts at: 0 1554 2925 Size: 1554 1371 BmrI ACTGGGnnnn_n' Cuts at: 0 1506 1560 1629 2925 Size: 1506 54 69 1296 Fragments arranged by size: 1506 1296 69 54 BmtI G_CTAG'C Cuts at: 0 2155 2925 Size: 2155 770 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 376 681 2673 2925 Size: 376 305 1992 252 Fragments arranged by size: 1992 376 305 252 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 309 358 820 1057 1118 1432 2572 Size: 50 259 49 462 237 61 314 1140 Cuts at: 2572 2698 2925 Size: 126 227 Fragments arranged by size: 1140 462 314 259 237 227 126 61 50 49 Bpu10I CC'TnA_GC Cuts at: 0 1300 1363 1641 1820 2925 Size: 1300 63 278 179 1105 Fragments arranged by size: 1300 1105 278 179 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1015 1882 2026 2121 2925 Size: 1015 867 144 95 804 Fragments arranged by size: 1015 867 804 144 95 BsaI GGTCTCn'nnnn_ Cuts at: 0 347 2674 2925 Size: 347 2327 251 Fragments arranged by size: 2327 347 251 BsaBI GATnn'nnATC Cuts at: 0 413 2906 2925 Size: 413 2493 19 Fragments arranged by size: 2493 413 19 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1615 1645 2416 2446 2925 Size: 1615 30 771 30 479 Fragments arranged by size: 1615 771 479 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1012 1075 2137 2173 2359 2925 Size: 1012 63 1062 36 186 566 Fragments arranged by size: 1062 1012 566 186 63 36 BseYI C'CCAG_C Cuts at: 0 2390 2466 2522 2925 Size: 2390 76 56 403 Fragments arranged by size: 2390 403 76 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 673 1127 2925 Size: 673 454 1798 Fragments arranged by size: 1798 673 454 BsiHKAI G_wGCw'C Cuts at: 0 224 602 2925 Size: 224 378 2323 Fragments arranged by size: 2323 378 224 BsmI GAATG_Cn' Cuts at: 0 990 2925 Size: 990 1935 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1030 2925 Size: 1030 1895 Bsp1286I G_dGCh'C Cuts at: 0 224 602 1554 2646 2925 Size: 224 378 952 1092 279 Fragments arranged by size: 1092 952 378 279 224 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 827 2925 Size: 827 2098 BsrBI CCG'CTC Cuts at: 0 925 2925 Size: 925 2000 BsrDI GCAATG_nn' Cuts at: 0 2516 2925 Size: 2516 409 BssSI C'ACGA_G Cuts at: 0 785 2925 Size: 785 2140 BstAPI GCAn_nnn'nTGC Cuts at: 0 2256 2925 Size: 2256 669 BstBI TT'CG_AA Cuts at: 0 843 2925 Size: 843 2082 BstYI r'GATC_y Cuts at: 0 708 2025 2046 2108 2315 2925 Size: 708 1317 21 62 207 610 Fragments arranged by size: 1317 708 610 207 62 21 BtgI C'CryG_G Cuts at: 0 2156 2925 Size: 2156 769 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 712 2893 2925 Size: 712 2181 32 Fragments arranged by size: 2181 712 32 BtsI GCAGTG_nn' Cuts at: 0 148 656 829 1115 2123 2925 Size: 148 508 173 286 1008 802 Fragments arranged by size: 1008 802 508 286 173 148 EaeI y'GGCC_r Cuts at: 0 485 2159 2925 Size: 485 1674 766 Fragments arranged by size: 1674 766 485 EarI CTCTTCn'nnn_ Cuts at: 0 126 497 2330 2411 2726 2925 Size: 126 371 1833 81 315 199 Fragments arranged by size: 1833 371 315 199 126 81 EcoICRI GAG'CTC Cuts at: 0 600 2925 Size: 600 2325 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 50 309 358 700 820 1057 1108 1118 Size: 50 259 49 342 120 237 51 10 Cuts at: 1118 1328 1432 2392 2572 2698 2925 Size: 210 104 960 180 126 227 Fragments arranged by size: 960 342 259 237 227 210 180 126 120 104 51 50 49 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 676 2925 Size: 676 2249 EcoO109I rG'GnC_Cy Cuts at: 0 1408 1479 1581 2477 2925 Size: 1408 71 102 896 448 Fragments arranged by size: 1408 896 448 102 71 EcoRI G'AATT_C Cuts at: 0 897 2925 Size: 897 2028 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 229 2772 2925 Size: 229 2543 153 Fragments arranged by size: 2543 229 153 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 247 279 496 498 528 530 589 621 Size: 247 32 217 2 30 2 59 32 Cuts at: 621 779 811 1612 1615 1644 1647 1990 2022 Size: 158 32 801 3 29 3 343 32 Cuts at: 2022 2925 Size: 903 Fragments arranged by size: 903 801 343 247 217 158 59 32 32 32 32 30 29 3 3 2 2 HincII GTy'rAC Cuts at: 0 279 2366 2925 Size: 279 2087 559 Fragments arranged by size: 2087 559 279 MfeI C'AATT_G Cuts at: 0 1882 2254 2925 Size: 1882 372 671 Fragments arranged by size: 1882 671 372 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 164 2000 2925 Size: 164 1836 925 Fragments arranged by size: 1836 925 164 MscI TGG'CCA Cuts at: 0 487 2161 2925 Size: 487 1674 764 Fragments arranged by size: 1674 764 487 MslI CAynn'nnrTG Cuts at: 0 46 1205 1847 2925 Size: 46 1159 642 1078 Fragments arranged by size: 1159 1078 642 46 MspA1I CmG'CkG Cuts at: 0 28 70 192 519 1467 1986 1996 2925 Size: 28 42 122 327 948 519 10 929 Fragments arranged by size: 948 929 519 327 122 42 28 10 NcoI C'CATG_G Cuts at: 0 2156 2925 Size: 2156 769 NheI G'CTAG_C Cuts at: 0 2151 2925 Size: 2151 774 NspI r_CATG'y Cuts at: 0 1152 2226 2628 2781 2925 Size: 1152 1074 402 153 144 Fragments arranged by size: 1152 1074 402 153 144 PciI A'CATG_T Cuts at: 0 2624 2925 Size: 2624 301 PfoI T'CCnGG_A Cuts at: 0 607 797 2925 Size: 607 190 2128 Fragments arranged by size: 2128 607 190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 845 877 1964 1996 2925 Size: 845 32 1087 32 929 Fragments arranged by size: 1087 929 845 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1479 1581 2477 2925 Size: 1479 102 896 448 Fragments arranged by size: 1479 896 448 102 PsiI TTA'TAA Cuts at: 0 911 2925 Size: 911 2014 PstI C_TGCA'G Cuts at: 0 524 824 1562 2925 Size: 524 300 738 1363 Fragments arranged by size: 1363 738 524 300 PvuII CAG'CTG Cuts at: 0 28 70 519 1467 1986 1996 2925 Size: 28 42 449 948 519 10 929 Fragments arranged by size: 948 929 519 449 42 28 10 SacI G_AGCT'C Cuts at: 0 602 2925 Size: 602 2323 ScaI AGT'ACT Cuts at: 0 1575 2925 Size: 1575 1350 SexAI A'CCwGG_T Cuts at: 0 1253 2357 2925 Size: 1253 1104 568 Fragments arranged by size: 1253 1104 568 SfcI C'TryA_G Cuts at: 0 520 694 820 1186 1558 2140 2395 2925 Size: 520 174 126 366 372 582 255 530 Fragments arranged by size: 582 530 520 372 366 255 174 126 SmlI C'TyrA_G Cuts at: 0 994 1897 2041 2100 2925 Size: 994 903 144 59 825 Fragments arranged by size: 994 903 825 144 59 SphI G_CATG'C Cuts at: 0 1152 2925 Size: 1152 1773 StuI AGG'CCT Cuts at: 0 2212 2925 Size: 2212 713 StyI C'CwwG_G Cuts at: 0 2156 2925 Size: 2156 769 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 449 2925 Size: 449 2476 TatI w'GTAC_w Cuts at: 0 1573 2925 Size: 1573 1352 XbaI T'CTAG_A Cuts at: 0 2659 2925 Size: 2659 266 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 504 2925 Size: 504 2421 XmnI GAAnn'nnTTC Cuts at: 0 667 1313 2925 Size: 667 646 1612 Fragments arranged by size: 1612 667 646 Enzymes that do cut: AclI AcuI AflIII AlfI AlwNI ApoI BaeI BanI BanII BbsI BbvCI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII MfeI MmeI MscI MslI MspA1I NcoI NheI NspI PciI PfoI PpiI PpuMI PsiI PstI PvuII SacI ScaI SexAI SfcI SmlI SphI StuI StyI TaqII TatI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BsaAI BsaHI BsaWI BsiEI BsiWI BspEI BspHI BsrFI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PflMI PmeI PmlI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TstI Tth111I XhoI XmaI ZraI