################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbrPI BcgI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FseI FspI HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 1161 AAATTT [GA]^AATT[CT] AfaI 291 GTAC GT^AC ApaI 945 GGGCCC GGGCC^C ApoI 1161 AAATTT [GA]^AATT[CT] Asp700I 198 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 102 GAGCTC G[AT]GC[AT]^C BbeI 178 GGCGCC GGCGC^C BbsI 838 GAAGAC GAAGAC(2/6) BbvCI 613 CCTCAGC CCTCAGC(-5/-2) BclI 1170 TGATCA T^GATCA BglI 55 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 838 GAAGAC GAAGAC(2/6) BsgI 901 GTGCAG GTGCAG(16/14) BsiHKAI 102 GAGCTC G[AT]GC[AT]^C BspMI 828 GCAGGT ACCTGC(4/8) BssSI 43 CTCGTG CACGAG(-5/-1) BstEII 525 GGTCACC G^GT[ACGT]ACC BstPI 525 GGTCACC G^GT[ACGT]ACC BstYI 767 AGATCC [GA]^GATC[CT] CfoI 179 GCGC GCG^C DrdI 958 GACCAGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 182 CCGTGG C^C[GA][CT]GG EaeI 50 TGGCCG [CT]^GGCC[GA] EcoO65I 525 GGTCACC G^GT[ACGT]ACC FauI 228 GCGGG CCCGC(4/6) FbaI 1170 TGATCA T^GATCA HaeII 178 GGCGCC [GA]GCGC^[CT] HhaI 179 GCGC GCG^C HinP1I 179 GCGC G^CGC HincII 280 GTCAAC GT[CT]^[GA]AC HindII 280 GTCAAC GT[CT]^[GA]AC KasI 178 GGCGCC G^GCGCC MaeII 674 ACGT A^CGT MflI 767 AGATCC [GA]^GATC[CT] NarI 178 GGCGCC GG^CGCC PvuII 268 CAGCTG CAG^CTG RsaI 291 GTAC GT^AC SacI 102 GAGCTC GAGCT^C SfoI 178 GGCGCC GGC^GCC StuI 636 AGGCCT AGG^CCT Tsp45I 526 GTCAC ^GT[GC]AC XcmI 710 CCAGTTCCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 767 AGATCC [GA]^GATC[CT] XmnI 198 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 17 GGCGTC G[GA]^CG[CT]C 178 GGCGCC AlwI 768 GATCC GGATC(4/5) 131 GGATC AlwNI 1024 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG 1070 CAGAATCTG AvaII 117 GGACC G^G[AT]CC 153 GGTCC BanI 178 GGCGCC G^G[CT][GA]CC 654 GGTGCC BanII 102 GAGCTC G[GA]GC[CT]^C 945 GGGCCC BmyI 102 GAGCTC G[AGT]GC[ACT]^C 945 GGGCCC BsaHI 17 GGCGTC G[GA]^CG[CT]C 178 GGCGCC BsaI 560 GAGACC GGTCTC(1/5) 956 GAGACC BsmFI 797 GGGAC GGGAC(10/14) 693 GTCCC Bsp1286I 102 GAGCTC G[AGT]GC[ACT]^C 945 GGGCCC DraII 944 AGGGCCC [GA]G^G[ACGT]CC[CT] 945 GGGCCCT EarI 520 GAAGAG CTCTTC(1/4) 857 GAAGAG EcoO109I 944 AGGGCCC [GA]G^G[ACGT]CC[CT] 945 GGGCCCT HgaI 18 GCGTC GACGC(5/10) 278 GCGTC Hin1I 17 GGCGTC G[GA]^CG[CT]C 178 GGCGCC Ksp632I 520 GAAGAG CTCTTC(1/4) 857 GAAGAG MaeIII 526 GTCAC ^GT[ACGT]AC 683 GTAAC MseI 367 TTAA T^TAA 1130 TTAA MslI 345 CATCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 648 CACAGAGGTG MspA1I 226 CAGCGG C[AC]G^C[GT]G 268 CAGCTG SfaNI 68 GCATC GCATC(5/9) 473 GCATC Tru9I 367 TTAA T^TAA 1130 TTAA Tsp509I 385 AATT ^AATT 1162 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI BbvI BcnI BpmI BsaJI BseRI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfcI TfiI TseI TspRI