(Circular) (Six-base) MAPSORT of: pFN21AE4380.seq Check: 8744 from: 1 to: 5905 pFN21AE4380 5905 bp With 182 enzymes: SgfI * March 28, 2012 14:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2824 2824 Size: 5905 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3248 1937 Size: 1311 4594 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2216 3968 5352 5620 1632 Size: 584 1752 1384 268 1917 Fragments arranged by size: 1917 1752 1384 584 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 4804 5781 1203 Size: 3601 977 1327 Fragments arranged by size: 3601 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 2657 1166 Size: 1491 4414 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3021 1184 Size: 1837 4068 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2438 2470 2438 Size: 32 5873 AlwNI CAG_nnn'CTG Cuts at: 3034 3080 3648 5220 3034 Size: 46 568 1572 3719 Fragments arranged by size: 3719 1572 568 46 ApaI G_GGCC'C Cuts at: 2954 2954 Size: 5905 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 3166 3214 3440 3592 5701 1493 Size: 1673 48 226 152 2109 1697 Fragments arranged by size: 2109 1697 1673 226 152 48 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 3230 1870 Size: 73 1287 4545 Fragments arranged by size: 4545 1287 73 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2183 2659 3226 4681 618 Size: 325 217 627 396 476 567 1455 1842 Fragments arranged by size: 1842 1455 627 567 476 396 325 217 BanII G_rGCy'C Cuts at: 1391 2111 2954 3224 1391 Size: 720 843 270 4072 Fragments arranged by size: 4072 843 720 270 BbeI G_GCGC'C Cuts at: 2187 2187 Size: 5905 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2850 928 Size: 971 951 3983 Fragments arranged by size: 3983 971 951 BbvCI CC'TCA_GC Cuts at: 2619 2619 Size: 5905 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4096 4130 1903 Size: 34 2159 34 3678 Fragments arranged by size: 3678 2159 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3797 5007 3797 Size: 1210 4695 BclI T'GATC_A Cuts at: 1548 3175 1548 Size: 1627 4278 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2066 4516 136 Size: 107 122 71 631 605 394 2450 1525 Fragments arranged by size: 2450 1525 631 605 394 122 107 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 3322 3322 Size: 5905 Bme1580I G_kGCm'C Cuts at: 1790 2954 3966 5122 1790 Size: 1164 1012 1156 2573 Fragments arranged by size: 2573 1164 1156 1012 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2709 2897 3636 Size: 416 53 83 399 24 1292 188 739 Cuts at: 3636 4594 442 Size: 958 1753 Fragments arranged by size: 1753 1292 958 739 416 399 188 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2111 2243 2297 2402 2465 4565 1239 Size: 872 132 54 105 63 2100 2579 Fragments arranged by size: 2579 2100 872 132 105 63 54 Bpu10I CC'TnA_GC Cuts at: 2468 2619 2955 2468 Size: 151 336 5418 Fragments arranged by size: 5418 336 151 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4030 4895 5193 4030 Size: 865 298 4742 Fragments arranged by size: 4742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2559 2955 4568 882 Size: 617 1060 396 1613 2219 Fragments arranged by size: 2219 1613 1060 617 396 BsaAI yAC'GTr Cuts at: 493 1204 3696 493 Size: 711 2492 2702 Fragments arranged by size: 2702 2492 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2023 2184 3712 4094 Size: 53 83 186 936 490 161 1528 382 Cuts at: 4094 4729 275 Size: 635 1451 Fragments arranged by size: 1528 1451 936 635 490 382 186 161 83 53 BsaWI w'CCGG_w Cuts at: 4338 4736 5010 5157 5568 4338 Size: 398 274 147 411 4675 Fragments arranged by size: 4675 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2438 2468 2583 2613 1178 Size: 30 165 30 1035 30 115 30 4470 Fragments arranged by size: 4470 1035 165 115 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2528 2804 3005 3008 3054 1186 Size: 1342 276 201 3 46 4037 Fragments arranged by size: 4037 1342 276 201 46 3 BseYI C'CCAG_C Cuts at: 5108 5604 5108 Size: 496 5409 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2927 1163 Size: 573 1191 4141 Fragments arranged by size: 4141 1191 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3355 4116 5144 1540 Size: 275 186 1354 761 1028 2301 Fragments arranged by size: 2301 1354 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2111 3966 4051 5122 2111 Size: 1855 85 1071 2894 Fragments arranged by size: 2894 1855 1071 85 BsmI GAATG_Cn' Cuts at: 3416 3509 3416 Size: 93 5812 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2111 2954 3224 3966 4051 5122 1391 Size: 399 321 843 270 742 85 1071 2174 Fragments arranged by size: 2174 1071 843 742 399 321 270 85 BspEI T'CCGG_A Cuts at: 5568 5568 Size: 5905 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2824 3260 844 Size: 1980 436 3489 Fragments arranged by size: 3489 1980 436 BsrBI CCG'CTC Cuts at: 1394 1823 3792 1394 Size: 429 1969 3507 Fragments arranged by size: 3507 1969 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4400 4574 65 Size: 1183 3152 174 1396 Fragments arranged by size: 3152 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4549 4736 1700 Size: 110 2739 187 2869 Fragments arranged by size: 2869 2739 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 2048 3965 4977 2048 Size: 1917 1012 2976 Fragments arranged by size: 2976 1917 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5905 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 2530 1184 Size: 1346 4559 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5905 BstYI r'GATC_y Cuts at: 1972 2772 3235 3602 3987 4004 5456 5542 5554 Size: 800 463 367 385 17 1452 86 12 Cuts at: 5554 5900 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 800 463 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2187 513 Size: 552 939 183 4231 Fragments arranged by size: 4231 939 552 183 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5166 Fragments arranged by size: 5166 683 56 BtsI GCAGTG_nn' Cuts at: 3421 4215 4235 3421 Size: 794 20 5091 Fragments arranged by size: 5091 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2969 4912 809 Size: 507 1653 1943 1802 Fragments arranged by size: 1943 1802 1653 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 3352 4241 Size: 54 1425 50 275 18 225 1297 889 Cuts at: 4241 5870 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1297 889 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3352 1537 Size: 275 1540 4090 Fragments arranged by size: 4090 1540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2520 2857 3835 1353 Size: 1167 337 978 3423 Fragments arranged by size: 3423 1167 978 337 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4492 4866 5012 387 Size: 1064 138 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2109 2109 Size: 5905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2216 2243 2297 2402 2465 3968 Size: 393 479 105 27 54 105 63 1503 Cuts at: 3968 4565 5352 5620 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1503 787 597 479 393 268 105 105 63 54 27 EcoO109I rG'GnC_Cy Cuts at: 2950 2951 2950 Size: 1 5904 EcoRI G'AATT_C Cuts at: 3214 3592 3214 Size: 378 5527 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 2187 5052 1678 Size: 509 2865 2531 Fragments arranged by size: 2865 2531 509 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2583 2615 4546 4578 4620 Size: 32 135 32 1011 32 1931 32 42 Cuts at: 4620 4652 1373 Size: 32 2626 Fragments arranged by size: 2626 1931 1011 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2287 3249 3495 669 Size: 988 281 349 962 246 3079 Fragments arranged by size: 3079 988 962 349 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5905 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KasI G'GCGC_C Cuts at: 2183 2183 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5019 5203 1305 Size: 3714 184 2007 Fragments arranged by size: 3714 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4083 Fragments arranged by size: 4083 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2354 2657 3863 4222 4381 518 Size: 648 1188 303 1206 359 159 2042 Fragments arranged by size: 2042 1206 1188 648 359 303 159 MspA1I CmG'CkG Cuts at: 1606 2233 2275 3321 3998 5146 5391 1606 Size: 627 42 1046 677 1148 245 2120 Fragments arranged by size: 2120 1148 1046 677 627 245 42 NarI GG'CG_CC Cuts at: 2184 2184 Size: 5905 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4414 Fragments arranged by size: 4414 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 3263 4808 1132 Size: 97 2034 1545 2229 Fragments arranged by size: 2229 2034 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5905 PfoI T'CCnGG_A Cuts at: 2111 2111 Size: 5905 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2438 2470 3974 4006 5487 5519 2438 Size: 32 1504 32 1481 32 2824 Fragments arranged by size: 2824 1504 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 2950 2950 Size: 5905 PstI C_TGCA'G Cuts at: 830 2176 2385 2406 2634 3257 830 Size: 1346 209 21 228 623 3478 Fragments arranged by size: 3478 1346 623 228 209 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 PvuII CAG'CTG Cuts at: 2275 2275 Size: 5905 SacI G_AGCT'C Cuts at: 2111 2111 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SfcI C'TryA_G Cuts at: 826 1045 2172 2381 2402 2553 2630 2853 3253 Size: 219 1127 209 21 151 77 223 400 Cuts at: 3253 4388 5069 5260 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1127 681 400 223 219 209 191 151 77 21 SfoI GGC'GCC Cuts at: 2185 2185 Size: 5905 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4009 4910 5172 820 Size: 197 926 2066 901 262 1553 Fragments arranged by size: 2066 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 2643 1517 Size: 1126 4779 StyI C'CwwG_G Cuts at: 513 1065 2004 3344 513 Size: 552 939 1340 3074 Fragments arranged by size: 3074 1340 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3932 3949 4102 4287 4732 325 Size: 1255 2352 17 153 185 445 1498 Fragments arranged by size: 2352 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4151 5863 96 Size: 274 80 33 51 1443 2174 1712 138 Fragments arranged by size: 2174 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5865 5897 5865 Size: 32 5873 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2722 2722 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 2207 4034 2207 Size: 1827 4078 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI