Restriction map for
pFN21AE3189
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrGI BsrI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI DpnII DraI DrdI
Eam1105I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NaeI NdeI NdeII NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI SalI Sau3AI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AflIII 284 ACATGT A^C[GA][CT]GT
Alw44I 1288 GTGCAC G^TGCAC
AlwNI 1069 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1288 GTGCAC G^TGCAC
BbvCI 622 CCTCAGC CCTCAGC(-5/-2)
BfaI 1285 CTAG C^TAG
BlpI 105 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 105 GCTGAGC GC^T[ACGT]AGC
BsgI 265 CTGCAC GTGCAG(16/14)
BsmBI 632 CGTCTC CGTCTC(1/5)
BspLU11I 284 ACATGT A^CATGT
BstAPI 1275 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 1181 CATCC GGATG(2/0)
CelII 105 GCTGAGC GC^T[ACGT]AGC
CpoI 390 CGGTCCG CG^G[AT]CCG
EarI 15 GAAGAG CTCTTC(1/4)
FokI 1181 CATCC GGATG(9/13)
Ksp632I 15 GAAGAG CTCTTC(1/4)
MaeI 1285 CTAG C^TAG
MaeIII 585 GTCAC ^GT[ACGT]AC
MslI 1083 CATGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 68 CCATGG C^CATGG
NspI 284 ACATGT [GA]CATG^[CT]
PleI 1001 GAGTC GAGTC(4/5)
PpuMI 1034 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 716 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 390 CGGTCCG CG^G[AT]CCG
SacI 18 GAGCTC GAGCT^C
SapI 15 GAAGAGC GCTCTTC(1/4)
TaqI 290 TCGA T^CGA
Tsp45I 585 GTCAC ^GT[GC]AC
TspRI 1146 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 290 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 24 GTAC GT^AC
529 GTAC
AvaII 391 GGTCC G^G[AT]CC
1035 GGTCC
BbeI 385 GGCGCC GGCGC^C
1176 GGCGCC
BpmI 857 CTGGAG CTGGAG(16/14)
1228 CTGGAG
BsmAI 633 GTCTC GTCTC(1/5)
723 GTCTC
BsrFI 388 GCCGGT [GA]^CCGG[CT]
1086 GCCGGT
BssHII 336 GCGCGC G^CGCGC
377 GCGCGC
BstNI 241 CCTGG CC^[AT]GG
891 CCTGG
Cfr10I 388 GCCGGT [GA]^CCGG[CT]
1086 GCCGGT
DraII 1024 GGGGCCT [GA]G^G[ACGT]CC[CT]
1034 GGGTCCC
DraIII 1185 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1276 CACTCGGTG
EcoO109I 1024 GGGGCCT [GA]G^G[ACGT]CC[CT]
1034 GGGTCCC
EcoRII 241 CCTGG ^CC[AT]GG
891 CCTGG
EcoT14I 68 CCATGG C^C[AT][AT]GG
869 CCTTGG
KasI 385 GGCGCC G^GCGCC
1176 GGCGCC
MboII 15 GAAGA GAAGA(8/7)
93 GAAGA
MvaI 241 CCTGG CC^[AT]GG
891 CCTGG
NarI 385 GGCGCC GG^CGCC
1176 GGCGCC
PstI 850 CTGCAG CTGCA^G
1132 CTGCAG
RsaI 24 GTAC GT^AC
529 GTAC
SfaNI 96 GATGC GCATC(5/9)
648 GATGC
SfoI 385 GGCGCC GGC^GCC
1176 GGCGCC
StyI 68 CCATGG C^C[AT][AT]GG
869 CCTTGG
TfiI 219 GATTC G^A[AT]TC
961 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AcyI AluI Aor51HI
AspHI BanI BanII BbvI BcnI
BglI BmyI BsaHI BsaJI BsiEI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BssKI BstUI Cac8I CfoI Cfr13I
DdeI DsaI EaeI EagI EclXI
Eco47III Eco52I FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KspI MaeII MnlI MspA1I MspI
MvnI MwoI NciI NlaIII NlaIV
NotI SacII Sau96I ScrFI SfcI
TseI