Restriction map for
pFN21AE3189
(Circular) (Six-base) MAPSORT of: pFN21AE3189.seq Check: 5051 from: 1 to: 6001
pFN21AE3189 6001 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3344 1937
Size: 1407 4594
Acc65I G'GTAC_C
Cuts at: 3322 3322
Size: 6001
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4064 5448 5716 1632
Size: 2432 1384 268 1917
Fragments arranged by size:
2432 1917 1384 268
AfeI AGC'GCT
Cuts at: 2141 2171 2327 2141
Size: 30 156 5815
Fragments arranged by size:
5815 156 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 2289 4900 5877 1203
Size: 1086 2611 977 1327
Fragments arranged by size:
2611 1327 1086 977
AgeI A'CCGG_T
Cuts at: 4832 4832
Size: 6001
AhdI GACnn_n'nnGTC
Cuts at: 4730 4730
Size: 6001
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2327 2829 1184
Size: 1143 502 4356
Fragments arranged by size:
4356 1143 502
AlwNI CAG_nnn'CTG
Cuts at: 3079 3744 5316 3079
Size: 665 1572 3764
Fragments arranged by size:
3764 1572 665
ApaLI G'TGCA_C
Cuts at: 3293 4058 5214 3293
Size: 765 1156 4080
Fragments arranged by size:
4080 1156 765
ApoI r'AATT_y
Cuts at: 1493 3310 3536 3688 5797 1493
Size: 1817 226 152 2109 1697
Fragments arranged by size:
2109 1817 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4555 160
Size: 872 3523 1606
Fragments arranged by size:
3523 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 3326 1870
Size: 73 1383 4545
Fragments arranged by size:
4545 1383 73
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2390 2406 3094 3181 3322
Size: 325 217 627 603 16 688 87 141
Cuts at: 3322 4777 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 688 627 603 325 217 141
87 16
BanII G_rGCy'C
Cuts at: 1391 2027 2756 3150 3240 3320 1391
Size: 636 729 394 90 80 4072
Fragments arranged by size:
4072 729 636 394 90 80
BbeI G_GCGC'C
Cuts at: 2394 3185 2394
Size: 791 5210
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5030
BbvCI CC'TCA_GC
Cuts at: 2628 2628
Size: 6001
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4192 4226 1903
Size: 34 50 34 2171 34 3678
Fragments arranged by size:
3678 2171 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3893 5103 3893
Size: 1210 4791
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2675 2777 2922
Size: 107 122 71 631 605 1003 102 145
Cuts at: 2922 2984 4612 136
Size: 62 1628 1525
Fragments arranged by size:
1628 1525 1003 631 605 145 122 107
102 71 62
BglII A'GATC_T
Cuts at: 5996 5996
Size: 6001
BlpI GC'TnA_GC
Cuts at: 2111 3418 2111
Size: 1307 4694
Bme1580I G_kGCm'C
Cuts at: 1790 2409 2913 3099 3297 4062 5218 1790
Size: 619 504 186 198 765 1156 2573
Fragments arranged by size:
2573 1156 765 619 504 198 186
BmgBI CAC'GTC
Cuts at: 1318 2793 1318
Size: 1475 4526
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3732 4690 442
Size: 416 53 83 399 24 2315 958 1753
Fragments arranged by size:
2315 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2883 3254 4661 1239
Size: 1644 371 1407 2579
Fragments arranged by size:
2579 1644 1407 371
Bpu10I CC'TnA_GC
Cuts at: 2628 2628
Size: 6001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4126 4991 5289 4126
Size: 865 298 4838
Fragments arranged by size:
4838 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4664 882
Size: 617 3165 2219
Fragments arranged by size:
3165 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3792 493
Size: 711 2588 2702
Fragments arranged by size:
2702 2588 711
BsaBI GATnn'nnATC
Cuts at: 5995 5995
Size: 6001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2317 2391 2722 2786
Size: 53 83 186 936 784 74 331 64
Cuts at: 2786 2879 2958 3182 3808 4190 4825 275
Size: 93 79 224 626 382 635 1451
Fragments arranged by size:
1451 936 784 635 626 382 331 224
186 93 83 79 74 64 53
BsaWI w'CCGG_w
Cuts at: 4434 4832 5106 5253 5664 4434
Size: 398 274 147 411 4771
Fragments arranged by size:
4771 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2183 2213 2997 3027 1178
Size: 30 165 30 780 30 784 30 4152
Fragments arranged by size:
4152 784 780 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6001
BseYI C'CCAG_C
Cuts at: 2493 2613 2749 5204 5700 2493
Size: 120 136 2455 496 2794
Fragments arranged by size:
2794 2455 496 136 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2255 1163
Size: 573 519 4909
Fragments arranged by size:
4909 573 519
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2015 2094 2670 2694 2760 2781
Size: 275 186 14 79 576 24 66 21
Cuts at: 2781 2988 3207 3258 3451 4212 5240 1540
Size: 207 219 51 193 761 1028 2301
Fragments arranged by size:
2301 1028 761 576 275 219 207 193
186 79 66 51 24 21 14
BsiHKAI G_wGCw'C
Cuts at: 2027 2163 3200 3297 4062 4147 5218 2027
Size: 136 1037 97 765 85 1071 2810
Fragments arranged by size:
2810 1071 1037 765 136 97 85
BsmI GAATG_Cn'
Cuts at: 3512 3605 3512
Size: 93 5908
BsmBI CGTCTCn'nnnn_
Cuts at: 2643 2643
Size: 6001
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2163 2409 2756 2913 3099 3150
Size: 399 237 136 246 347 157 186 51
Cuts at: 3150 3200 3240 3297 3320 4062 4147 5218 1391
Size: 50 40 57 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 347 246 237 186
157 136 85 57 51 50 40 23
BspEI T'CCGG_A
Cuts at: 5664 5664
Size: 6001
BspHI T'CATG_A
Cuts at: 3890 5620 3890
Size: 1730 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3356 844
Size: 2512 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3888 1394
Size: 429 2065 3507
Fragments arranged by size:
3507 2065 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4496 4670 65
Size: 1183 3248 174 1396
Fragments arranged by size:
3248 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2393 3091 4645 4832 1700
Size: 110 583 698 1554 187 2869
Fragments arranged by size:
2869 1554 698 583 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 2341 2382 1925
Size: 416 41 5544
Fragments arranged by size:
5544 416 41
BssSI C'ACGA_G
Cuts at: 4061 5073 4061
Size: 1012 4989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3286 794
Size: 2492 3509
BstBI TT'CG_AA
Cuts at: 4805 4805
Size: 6001
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6001
BstYI r'GATC_y
Cuts at: 1972 3331 3698 4083 4100 5552 5638 5650 5996
Size: 1359 367 385 17 1452 86 12 346
Cuts at: 5996 1972
Size: 1977
Fragments arranged by size:
1977 1452 1359 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2009 2073 2228 2670 2694 2703 2775
Size: 552 944 64 155 442 24 9 72
Cuts at: 2775 2781 2982 3159 3201 3207 513
Size: 6 201 177 42 6 3307
Fragments arranged by size:
3307 944 552 442 201 177 155 72
64 42 24 9 6 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5262
Fragments arranged by size:
5262 683 56
BtsI GCAGTG_nn'
Cuts at: 3517 4311 4331 3517
Size: 794 20 5187
Fragments arranged by size:
5187 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6001
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DraIII CAC_nnn'GTG
Cuts at: 3195 3286 3195
Size: 91 5910
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5008 809
Size: 507 3692 1802
Fragments arranged by size:
3692 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2012 2091 2667
Size: 54 1425 50 275 18 182 79 576
Cuts at: 2667 2691 2757 2778 2833 2914 2945 2973 2985
Size: 24 66 21 55 81 31 28 12
Cuts at: 2985 3204 3255 3448 4337 5966 8
Size: 219 51 193 889 1629 43
Fragments arranged by size:
1629 1425 889 576 275 219 193 182
81 79 66 55 54 51 50 43
31 28 24 21 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2012 2091 2667 2691 2757 2778 2985
Size: 275 200 79 576 24 66 21 207
Cuts at: 2985 3204 3255 3448 1537
Size: 219 51 193 4090
Fragments arranged by size:
4090 576 275 219 207 200 193 79
66 51 24 21
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 3931 1353
Size: 662 1916 3423
Fragments arranged by size:
3423 1916 662
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4588 4962 5108 387
Size: 1064 138 2999 374 146 1280
Fragments arranged by size:
2999 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2025 2025
Size: 6001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2883 3254 4064 4661 5448 5716 1239
Size: 393 1251 371 810 597 787 268 1524
Fragments arranged by size:
1524 1251 810 787 597 393 371 268
EcoO109I rG'GnC_Cy
Cuts at: 3030 3040 3030
Size: 10 5991
EcoRI G'AATT_C
Cuts at: 3310 3688 3310
Size: 378 5623
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 2827 2275
Size: 552 5449
FspI TGC'GCA
Cuts at: 4507 4507
Size: 6001
HaeII r_GCGC'y
Cuts at: 1678 2143 2173 2270 2329 2394 3185 5148 1678
Size: 465 30 97 59 65 791 1963 2531
Fragments arranged by size:
2531 1963 791 465 97 65 59 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4642 4674 4716 4748 1373
Size: 32 135 32 3070 32 42 32 2626
Fragments arranged by size:
3070 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3345 3591 669
Size: 988 281 1407 246 3079
Fragments arranged by size:
3079 1407 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6001
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KasI G'GCGC_C
Cuts at: 2390 3181 2390
Size: 791 5210
KpnI G_GTAC'C
Cuts at: 3326 3326
Size: 6001
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5115 5299 1305
Size: 3810 184 2007
Fragments arranged by size:
3810 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4179
Fragments arranged by size:
4179 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3092 3959 4318 4477 518
Size: 648 1926 867 359 159 2042
Fragments arranged by size:
2042 1926 867 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2110 2230 2696 2705 2768 2777 2783
Size: 405 99 120 466 9 63 9 6
Cuts at: 2783 2984 3161 3203 3209 3417 4094 5242 5487
Size: 201 177 42 6 208 677 1148 245
Cuts at: 5487 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 466 405 245 208 201
177 120 99 63 42 9 9 6
6
NarI GG'CG_CC
Cuts at: 2391 3182 2391
Size: 791 5210
NcoI C'CATG_G
Cuts at: 513 1065 2073 513
Size: 552 1008 4441
Fragments arranged by size:
4441 1008 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 2691 2778 2985 3204 3448 2691
Size: 87 207 219 244 5244
Fragments arranged by size:
5244 244 219 207 87
NspI r_CATG'y
Cuts at: 1132 1229 2293 3359 4904 1132
Size: 97 1064 1066 1545 2229
Fragments arranged by size:
2229 1545 1066 1064 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6001
PciI A'CATG_T
Cuts at: 2289 4900 2289
Size: 2611 3390
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6001
PfoI T'CCnGG_A
Cuts at: 2398 2398
Size: 6001
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 5583 5615 4070
Size: 32 1481 32 4456
Fragments arranged by size:
4456 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3040 3040
Size: 6001
PshAI GACnn'nnGTC
Cuts at: 1613 2725 1613
Size: 1112 4889
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PstI C_TGCA'G
Cuts at: 830 2859 3141 3353 830
Size: 2029 282 212 3478
Fragments arranged by size:
3478 2029 282 212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
RsrII CG'GwC_CG
Cuts at: 2396 2396
Size: 6001
SacI G_AGCT'C
Cuts at: 2027 2027
Size: 6001
SacII CC_GC'GG
Cuts at: 2012 2231 2697 2706 2778 2784 2985 3162 3204
Size: 219 466 9 72 6 201 177 42
Cuts at: 3204 3210 2012
Size: 6 4803
Fragments arranged by size:
4803 466 219 201 177 72 42 9
6 6
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SanDI GG'GwC_CC
Cuts at: 3040 3040
Size: 6001
SapI GCTCTTCn'nnn_
Cuts at: 2015 2015
Size: 6001
SbfI CC_TGCA'GG
Cuts at: 3353 3353
Size: 6001
ScaI AGT'ACT
Cuts at: 4249 5961 4249
Size: 1712 4289
SfcI C'TryA_G
Cuts at: 826 1045 2855 3012 3137 3349 4484 5165 5356
Size: 219 1810 157 125 212 1135 681 191
Cuts at: 5356 826
Size: 1471
Fragments arranged by size:
1810 1471 1135 681 219 212 191 157
125
SfoI GGC'GCC
Cuts at: 2392 3183 2392
Size: 791 5210
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 3328 1872
Size: 1456 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4105 5006 5268 820
Size: 197 926 2162 901 262 1553
Fragments arranged by size:
2162 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 3359 1132
Size: 2227 3774
SspI AAT'ATT
Cuts at: 5 52 3925 5
Size: 47 3873 2081
Fragments arranged by size:
3873 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6001
StyI C'CwwG_G
Cuts at: 513 1065 2073 2874 3440 513
Size: 552 1008 801 566 3074
Fragments arranged by size:
3074 1008 801 566 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2502 4028 4045 4198 4383 4828 325
Size: 1255 922 1526 17 153 185 445 1498
Fragments arranged by size:
1526 1498 1255 922 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4247 5959 96
Size: 274 80 33 51 1443 2270 1712 138
Fragments arranged by size:
2270 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2183 2215 5961 5993 2183
Size: 32 3746 32 2191
Fragments arranged by size:
3746 2191 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 3326 1870
Size: 1456 4545
XmnI GAAnn'nnTTC
Cuts at: 4130 4130
Size: 6001
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII SexAI SfiI SrfI SwaI XcmI