(Linear) (Six-base) MAPSORT of: KIEE3189.seq Check: 2036 from: 1 to: 1296 KIEE3189.orf With 182 enzymes: SgfI * January 29, 2014 11:45 .. AfeI AGC'GCT Cuts at: 0 136 166 322 1296 Size: 136 30 156 974 Fragments arranged by size: 974 156 136 30 AflIII A'CryG_T Cuts at: 0 284 1296 Size: 284 1012 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 322 824 1296 Size: 322 502 472 Fragments arranged by size: 502 472 322 AlwNI CAG_nnn'CTG Cuts at: 0 1074 1296 Size: 1074 222 ApaLI G'TGCA_C Cuts at: 0 1288 1296 Size: 1288 8 BanI G'GyrC_C Cuts at: 0 385 401 1089 1176 1296 Size: 385 16 688 87 120 Fragments arranged by size: 688 385 120 87 16 BanII G_rGCy'C Cuts at: 0 22 751 1145 1235 1296 Size: 22 729 394 90 61 Fragments arranged by size: 729 394 90 61 22 BbeI G_GCGC'C Cuts at: 0 389 1180 1296 Size: 389 791 116 Fragments arranged by size: 791 389 116 BbvCI CC'TCA_GC Cuts at: 0 623 1296 Size: 623 673 BglI GCCn_nnn'nGGC Cuts at: 0 670 772 917 979 1296 Size: 670 102 145 62 317 Fragments arranged by size: 670 317 145 102 62 BlpI GC'TnA_GC Cuts at: 0 106 1296 Size: 106 1190 Bme1580I G_kGCm'C Cuts at: 0 404 908 1094 1292 1296 Size: 404 504 186 198 4 Fragments arranged by size: 504 404 198 186 4 BmgBI CAC'GTC Cuts at: 0 788 1296 Size: 788 508 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 878 1249 1296 Size: 878 371 47 Fragments arranged by size: 878 371 47 Bpu10I CC'TnA_GC Cuts at: 0 623 1296 Size: 623 673 BsaHI Gr'CG_yC Cuts at: 0 312 386 717 781 874 953 1177 1296 Size: 312 74 331 64 93 79 224 119 Fragments arranged by size: 331 312 224 119 93 79 74 64 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 178 208 992 1022 1296 Size: 178 30 784 30 274 Fragments arranged by size: 784 274 178 30 30 BseYI C'CCAG_C Cuts at: 0 488 608 744 1296 Size: 488 120 136 552 Fragments arranged by size: 552 488 136 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 1296 Size: 250 1046 BsiEI CG_ry'CG Cuts at: 0 10 89 665 689 755 776 983 1202 Size: 10 79 576 24 66 21 207 219 Cuts at: 1202 1253 1296 Size: 51 43 Fragments arranged by size: 576 219 207 79 66 51 43 24 21 10 BsiHKAI G_wGCw'C Cuts at: 0 22 158 1195 1292 1296 Size: 22 136 1037 97 4 Fragments arranged by size: 1037 136 97 22 4 BsmBI CGTCTCn'nnnn_ Cuts at: 0 638 1296 Size: 638 658 Bsp1286I G_dGCh'C Cuts at: 0 22 158 404 751 908 1094 1145 1195 Size: 22 136 246 347 157 186 51 50 Cuts at: 1195 1235 1292 1296 Size: 40 57 4 Fragments arranged by size: 347 246 186 157 136 57 51 50 40 22 4 BsrFI r'CCGG_y Cuts at: 0 388 1086 1296 Size: 388 698 210 Fragments arranged by size: 698 388 210 BssHII G'CGCG_C Cuts at: 0 336 377 1296 Size: 336 41 919 Fragments arranged by size: 919 336 41 BstAPI GCAn_nnn'nTGC Cuts at: 0 1281 1296 Size: 1281 15 BtgI C'CryG_G Cuts at: 0 4 68 223 665 689 698 770 776 Size: 4 64 155 442 24 9 72 6 Cuts at: 776 977 1154 1196 1202 1296 Size: 201 177 42 6 94 Fragments arranged by size: 442 201 177 155 94 72 64 42 24 9 6 6 4 DraIII CAC_nnn'GTG Cuts at: 0 1190 1281 1296 Size: 1190 91 15 Fragments arranged by size: 1190 91 15 EaeI y'GGCC_r Cuts at: 0 7 86 662 686 752 773 828 909 Size: 7 79 576 24 66 21 55 81 Cuts at: 909 940 968 980 1199 1250 1296 Size: 31 28 12 219 51 46 Fragments arranged by size: 576 219 81 79 66 55 51 46 31 28 24 21 12 7 EagI C'GGCC_G Cuts at: 0 7 86 662 686 752 773 980 1199 Size: 7 79 576 24 66 21 207 219 Cuts at: 1199 1250 1296 Size: 51 46 Fragments arranged by size: 576 219 207 79 66 51 46 24 21 7 EarI CTCTTCn'nnn_ Cuts at: 0 10 1296 Size: 10 1286 EcoICRI GAG'CTC Cuts at: 0 20 1296 Size: 20 1276 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 878 1249 1296 Size: 878 371 47 Fragments arranged by size: 878 371 47 EcoO109I rG'GnC_Cy Cuts at: 0 1025 1035 1296 Size: 1025 10 261 Fragments arranged by size: 1025 261 10 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 270 822 1296 Size: 270 552 474 Fragments arranged by size: 552 474 270 HaeII r_GCGC'y Cuts at: 0 138 168 265 324 389 1180 1296 Size: 138 30 97 59 65 791 116 Fragments arranged by size: 791 138 116 97 65 59 30 KasI G'GCGC_C Cuts at: 0 385 1176 1296 Size: 385 791 120 Fragments arranged by size: 791 385 120 MslI CAynn'nnrTG Cuts at: 0 1087 1296 Size: 1087 209 MspA1I CmG'CkG Cuts at: 0 6 105 225 691 700 763 772 778 Size: 6 99 120 466 9 63 9 6 Cuts at: 778 979 1156 1198 1204 1296 Size: 201 177 42 6 92 Fragments arranged by size: 466 201 177 120 99 92 63 42 9 9 6 6 6 NarI GG'CG_CC Cuts at: 0 386 1177 1296 Size: 386 791 119 Fragments arranged by size: 791 386 119 NcoI C'CATG_G Cuts at: 0 68 1296 Size: 68 1228 NotI GC'GGCC_GC Cuts at: 0 686 773 980 1199 1296 Size: 686 87 207 219 97 Fragments arranged by size: 686 219 207 97 87 NspI r_CATG'y Cuts at: 0 288 1296 Size: 288 1008 PciI A'CATG_T Cuts at: 0 284 1296 Size: 284 1012 PfoI T'CCnGG_A Cuts at: 0 393 1296 Size: 393 903 PpuMI rG'GwC_Cy Cuts at: 0 1035 1296 Size: 1035 261 PshAI GACnn'nnGTC Cuts at: 0 720 1296 Size: 720 576 PstI C_TGCA'G Cuts at: 0 854 1136 1296 Size: 854 282 160 Fragments arranged by size: 854 282 160 RsrII CG'GwC_CG Cuts at: 0 391 1296 Size: 391 905 SacI G_AGCT'C Cuts at: 0 22 1296 Size: 22 1274 SacII CC_GC'GG Cuts at: 0 7 226 692 701 773 779 980 1157 Size: 7 219 466 9 72 6 201 177 Cuts at: 1157 1199 1205 1296 Size: 42 6 91 Fragments arranged by size: 466 219 201 177 91 72 42 9 7 6 6 SanDI GG'GwC_CC Cuts at: 0 1035 1296 Size: 1035 261 SapI GCTCTTCn'nnn_ Cuts at: 0 10 1296 Size: 10 1286 SfcI C'TryA_G Cuts at: 0 850 1007 1132 1296 Size: 850 157 125 164 Fragments arranged by size: 850 164 157 125 SfoI GGC'GCC Cuts at: 0 387 1178 1296 Size: 387 791 118 Fragments arranged by size: 791 387 118 StyI C'CwwG_G Cuts at: 0 68 869 1296 Size: 68 801 427 Fragments arranged by size: 801 427 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 497 1296 Size: 497 799 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 178 210 1296 Size: 178 32 1086 Fragments arranged by size: 1086 178 32 Enzymes that do cut: AfeI AflIII AlfI AlwNI ApaLI BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BssHII BstAPI BtgI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI HaeII KasI MslI MspA1I NarI NcoI NotI NspI PciI PfoI PpuMI PshAI PstI RsrII SacI SacII SanDI SapI SfcI SfoI StyI TaqII TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AgeI AhdI AleI AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EciI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI NdeI NgoMIV NheI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI PvuII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI