Restriction map for
pFN21AE5971
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BsaHI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
CfoI ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoNI EcoRI EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KspI MfeI
MluI MroI MseI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 392 GGTACC G^GTACC
AflIII 572 ACACGT A^C[GA][CT]GT
AlwI 540 GATCC GGATC(4/5)
AlwNI 696 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 392 GGTACC G^GTACC
BcnI 263 CCCGG CC^[GC]GG
BfaI 151 CTAG C^TAG
BlpI 92 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 92 GCTCAGC GC^T[ACGT]AGC
BsaBI 477 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 457 CTCCTC GAGGAG(10/8)
BsgI 163 CTGCAC GTGCAG(16/14)
BsiEI 689 CGGCCG CG[GA][CT]^CG
BsmI 634 GCATTC GAATGC(1/-1)
BspHI 404 TCATGA T^CATGA
BsrBI 7 CCGCTC CCGCTC(-3/-3)
BsrFI 413 ACCGGC [GA]^CCGG[CT]
BsrI 342 ACTGG ACTGG(1/-1)
BstEII 108 GGTGACC G^GT[ACGT]ACC
BstPI 108 GGTGACC G^GT[ACGT]ACC
BstYI 539 AGATCC [GA]^GATC[CT]
CelII 92 GCTCAGC GC^T[ACGT]AGC
Cfr10I 413 ACCGGC [GA]^CCGG[CT]
DpnII 540 GATC ^GATC
DraII 446 GGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 689 CGGCCG C^GGCCG
EarI 79 CTCTTC CTCTTC(1/4)
EclXI 689 CGGCCG C^GGCCG
Eco52I 689 CGGCCG C^GGCCG
EcoO109I 446 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 108 GGTGACC G^GT[ACGT]ACC
EcoRV 154 GATATC GAT^ATC
EcoT14I 836 CCATGG C^C[AT][AT]GG
FauI 601 GCGGG CCCGC(4/6)
HinfI 921 GAGTC G^A[ACGT]TC
KpnI 392 GGTACC GGTAC^C
Ksp632I 79 CTCTTC CTCTTC(1/4)
MaeI 151 CTAG C^TAG
MamI 477 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 540 GATC ^GATC
MflI 539 AGATCC [GA]^GATC[CT]
NciI 263 CCCGG CC^[GC]GG
NcoI 836 CCATGG C^CATGG
NdeII 540 GATC ^GATC
NspI 562 GCATGC [GA]CATG^[CT]
PflMI 510 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 921 GAGTC GAGTC(4/5)
PpuMI 446 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 404 TCATGA T^CATGA
SapI 78 GCTCTTC GCTCTTC(1/4)
Sau3AI 540 GATC ^GATC
SexAI 104 ACCTGGT A^CC[AT]GGT
SfiI 345 GGCCATCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 460 CTCAAG C^T[CT][GA]AG
SphI 562 GCATGC GCATG^C
StyI 836 CCATGG C^C[AT][AT]GG
TspRI 548 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 510 CCACCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 539 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 438 AGCT AG^CT
557 AGCT
AocI 675 CCTGAGG CC^T[ACGT]AGG
873 CCTGAGG
AspHI 90 GTGCTC G[AT]GC[AT]^C
400 GTGCTC
AvaII 246 GGTCC G^G[AT]CC
447 GGTCC
BanII 321 GGGCTC G[GA]GC[CT]^C
603 GGGCTC
BbrPI 397 CACGTG CAC^GTG
514 CACGTG
BglI 130 GCCATCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
346 GCCATCACGGC
BpmI 930 CTCCAG CTGGAG(16/14)
67 CTGGAG
BsaAI 397 CACGTG [CT]AC^GT[GA]
514 CACGTG
BsiHKAI 90 GTGCTC G[AT]GC[AT]^C
400 GTGCTC
BstXI 98 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
716 CCACCTGCTTGG
Bsu36I 675 CCTGAGG CC^T[ACGT]AGG
873 CCTGAGG
DraIII 327 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
511 CACCACGTG
Eco81I 675 CCTGAGG CC^T[ACGT]AGG
873 CCTGAGG
HapII 264 CCGG C^CGG
414 CCGG
HpaII 264 CCGG C^CGG
414 CCGG
MspI 264 CCGG C^CGG
414 CCGG
PmaCI 397 CACGTG CAC^GTG
514 CACGTG
PmlI 397 CACGTG CAC^GTG
514 CACGTG
SfaNI 240 GATGC GCATC(5/9)
72 GCATC
SfcI 737 CTGTAG C^T[GA][CT]AG
867 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI BalI BanI BbsI
BbvI BmyI BpuAI BsaJI BsiYI
BslI Bsp1286I BspMI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DsaI
EaeI EcoRII Fnu4HI FokI HaeIII
HphI ItaI MaeII MaeIII MboII
MluNI MnlI MscI MslI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI TseI Tsp45I