Restriction map for
pFN21AE0797
(Circular) (Six-base) MAPSORT of: pFN21AE0797.seq Check: 4690 from: 1 to: 9421
pFN21AE0797 9421 bp
With 182 enzymes: SgfI *
September 9, 2010 14:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7231 278
Size: 53 83 186 936 5695 2468
Fragments arranged by size:
5695 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2242 4816 6764 1937
Size: 305 2574 1948 4594
Fragments arranged by size:
4594 2574 1948 305
Acc65I G'GTAC_C
Cuts at: 6742 6742
Size: 9421
AclI AA'CG_TT
Cuts at: 1563 7548 7921 1563
Size: 5985 373 3063
Fragments arranged by size:
5985 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3038 3549 4874 5331 5351 5393 5892 7484
Size: 1406 511 1325 457 20 42 499 1592
Cuts at: 7484 8868 9136 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1592 1406 1384 1325 511 499 457
268 42 20
AflII C'TTAA_G
Cuts at: 820 1017 6032 820
Size: 197 5015 4209
Fragments arranged by size:
5015 4209 197
AflIII A'CryG_T
Cuts at: 1203 4911 8320 9297 1203
Size: 3708 3409 977 1327
Fragments arranged by size:
3708 3409 1327 977
AgeI A'CCGG_T
Cuts at: 8252 8252
Size: 9421
AhdI GACnn_n'nnGTC
Cuts at: 5623 8150 5623
Size: 2527 6894
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9421
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2315 4101 4539 6483 1184
Size: 1131 1786 438 1944 4122
Fragments arranged by size:
4122 1944 1786 1131 438
AlwNI CAG_nnn'CTG
Cuts at: 2024 2836 3271 4198 5445 5464 7164 8736 2024
Size: 812 435 927 1247 19 1700 1572 2709
Fragments arranged by size:
2709 1700 1572 1247 927 812 435 19
ApaI G_GGCC'C
Cuts at: 2327 2470 2919 4684 2327
Size: 143 449 1765 7064
Fragments arranged by size:
7064 1765 449 143
ApaLI G'TGCA_C
Cuts at: 7478 8634 7478
Size: 1156 8265
ApoI r'AATT_y
Cuts at: 1493 3027 4376 4469 5336 5750 6730 6956 7108
Size: 1534 1349 93 867 414 980 226 152
Cuts at: 7108 9217 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1534 1349 980 867 414 226
152 93
AseI AT'TA_AT
Cuts at: 160 1032 4164 6275 7975 160
Size: 872 3132 2111 1700 1606
Fragments arranged by size:
3132 2111 1700 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9421
AvaI C'yCGr_G
Cuts at: 1870 1943 2583 2797 2818 2912 2938 6746 1870
Size: 73 640 214 21 94 26 3808 4545
Fragments arranged by size:
4545 3808 640 214 94 73 26 21
AvrII C'CTAG_G
Cuts at: 5101 6340 5101
Size: 1239 8182
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5412 5445 5412
Size: 33 9388
BamHI G'GATC_C
Cuts at: 2279 3991 4632 4959 5351 6404 6751 7118 2279
Size: 1712 641 327 392 1053 347 367 4582
Fragments arranged by size:
4582 1712 1053 641 392 367 347 327
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2842 5297 6742 8197 618
Size: 325 217 627 1055 2455 1445 1455 1842
Fragments arranged by size:
2455 1842 1455 1445 1055 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2327 2395 2470 2557 2919 3753 4097 4393
Size: 936 68 75 87 362 834 344 296
Cuts at: 4393 4684 5485 6740 1391
Size: 291 801 1255 4072
Fragments arranged by size:
4072 1255 936 834 801 362 344 296
291 87 75 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2271 5619 6000 6220 6426 928
Size: 971 372 3348 381 220 206 3923
Fragments arranged by size:
3923 3348 971 381 372 220 206
BbvCI CC'TCA_GC
Cuts at: 3702 3702
Size: 9421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2531 2565 7612 7646 1903
Size: 34 594 34 5047 34 3678
Fragments arranged by size:
5047 3678 594 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3069 7313 8523 3069
Size: 4244 1210 3967
Fragments arranged by size:
4244 3967 1210
BclI T'GATC_A
Cuts at: 1548 3559 4803 4899 1548
Size: 2011 1244 96 6070
Fragments arranged by size:
6070 2011 1244 96
BfrBI ATG'CAT
Cuts at: 2215 2224 2215
Size: 9 9412
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5295 8032 136
Size: 107 122 71 631 605 3623 2737 1525
Fragments arranged by size:
3623 2737 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3899 6411 9416 3899
Size: 2512 3005 3904
Fragments arranged by size:
3904 3005 2512
BlpI GC'TnA_GC
Cuts at: 2810 4026 4047 5375 6838 2810
Size: 1216 21 1328 1463 5393
Fragments arranged by size:
5393 1463 1328 1216 21
Bme1580I G_kGCm'C
Cuts at: 1790 2294 2327 2470 2847 2913 2919 4684 6457
Size: 504 33 143 377 66 6 1765 1773
Cuts at: 6457 7482 8638 1790
Size: 1025 1156 2573
Fragments arranged by size:
2573 1773 1765 1156 1025 504 377 143
66 33 6
BmgBI CAC'GTC
Cuts at: 1318 2404 4057 5944 1318
Size: 1086 1653 1887 4795
Fragments arranged by size:
4795 1887 1653 1086
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4093 4653 5350
Size: 416 53 83 399 24 2676 560 697
Cuts at: 5350 7152 8110 442
Size: 1802 958 1753
Fragments arranged by size:
2676 1802 1753 958 697 560 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9421
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 3297 5863 2422
Size: 875 2566 5980
Fragments arranged by size:
5980 2566 875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2392 2442 2945 3242 3320 4175 6371 6419
Size: 1153 50 503 297 78 855 2196 48
Cuts at: 6419 8081 1239
Size: 1662 2579
Fragments arranged by size:
2579 2196 1662 1153 855 503 297 78
50 48
Bpu10I CC'TnA_GC
Cuts at: 3702 5981 3702
Size: 2279 7142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7546 8411 8709 7546
Size: 865 298 8258
Fragments arranged by size:
8258 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 8084 882
Size: 617 6585 2219
Fragments arranged by size:
6585 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 7212 493
Size: 711 6008 2702
Fragments arranged by size:
6008 2702 711
BsaBI GATnn'nnATC
Cuts at: 6066 9415 6066
Size: 3349 6072
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3858 4813 5958 7228
Size: 53 83 186 936 2325 955 1145 1270
Cuts at: 7228 7610 8245 275
Size: 382 635 1451
Fragments arranged by size:
2325 1451 1270 1145 955 936 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3739 5704 6333 7854 8252 8526 8673 9084 3739
Size: 1965 629 1521 398 274 147 411 4076
Fragments arranged by size:
4076 1965 1521 629 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4133 4163 5960 5990 1178
Size: 30 165 30 2730 30 1797 30 4609
Fragments arranged by size:
4609 2730 1797 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2439 3551 3694 3697 3700 3719 4676 4688
Size: 1253 1112 143 3 3 19 957 12
Cuts at: 4688 5690 5846 5915 5918 6521 6686 1186
Size: 1002 156 69 3 603 165 3921
Fragments arranged by size:
3921 1253 1112 1002 957 603 165 156
143 69 19 12 3 3 3
BseYI C'CCAG_C
Cuts at: 4452 4872 8624 9120 4452
Size: 420 3752 496 4753
Fragments arranged by size:
4753 3752 496 420
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8848
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2860 2904 5565 6871 7632 8660
Size: 275 186 859 44 2661 1306 761 1028
Cuts at: 8660 1540
Size: 2301
Fragments arranged by size:
2661 2301 1306 1028 859 761 275 186
44
BsiHKAI G_wGCw'C
Cuts at: 2403 2557 2825 3645 4311 5381 5485 7482 7567
Size: 154 268 820 666 1070 104 1997 85
Cuts at: 7567 8638 2403
Size: 1071 3186
Fragments arranged by size:
3186 1997 1071 1070 820 666 268 154
104 85
BsmI GAATG_Cn'
Cuts at: 3516 3715 5648 6932 7025 3516
Size: 199 1933 1284 93 5912
Fragments arranged by size:
5912 1933 1284 199 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2410 3218 2410
Size: 808 8613
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2294 2327 2395 2403 2470 2557 2825
Size: 399 504 33 68 8 67 87 268
Cuts at: 2825 2847 2913 2919 3645 3753 4097 4311 4393
Size: 22 66 6 726 108 344 214 82
Cuts at: 4393 4684 5381 5485 6457 6740 7482 7567 8638
Size: 291 697 104 972 283 742 85 1071
Cuts at: 8638 1391
Size: 2174
Fragments arranged by size:
2174 1071 972 742 726 697 504 399
344 291 283 268 214 108 104 87
85 82 68 67 66 33 22 8
6
BspEI T'CCGG_A
Cuts at: 9084 9084
Size: 9421
BspHI T'CATG_A
Cuts at: 5238 7310 9040 5238
Size: 2072 1730 5619
Fragments arranged by size:
5619 2072 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6776 844
Size: 5932 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2814 3040 3493 5641 7308 1394
Size: 429 991 226 453 2148 1667 3507
Fragments arranged by size:
3507 2148 1667 991 453 429 226
BsrDI GCAATG_nn'
Cuts at: 65 1248 4281 6505 7916 8090 65
Size: 1183 3033 2224 1411 174 1396
Fragments arranged by size:
3033 2224 1411 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2764 2839 2859 2932 3809 5781 8065
Size: 110 954 75 20 73 877 1972 2284
Cuts at: 8065 8252 1700
Size: 187 2869
Fragments arranged by size:
2869 2284 1972 954 877 187 110 75
73 20
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9421
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9421
BssSI C'ACGA_G
Cuts at: 5636 7481 8493 5636
Size: 1845 1012 6564
Fragments arranged by size:
6564 1845 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2221 2836 794
Size: 1427 615 7379
Fragments arranged by size:
7379 1427 615
BstBI TT'CG_AA
Cuts at: 8225 8225
Size: 9421
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9421
BstXI CCAn_nnnn'nTGG
Cuts at: 3442 3442
Size: 9421
BstYI r'GATC_y
Cuts at: 1972 2279 3420 3899 3991 4632 4959 5351 5461
Size: 307 1141 479 92 641 327 392 110
Cuts at: 5461 5553 6067 6404 6411 6751 7118 7503 7520
Size: 92 514 337 7 340 367 385 17
Cuts at: 7520 8972 9058 9070 9416 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1141 641 514 479 392 385
367 346 340 337 327 307 110 92
92 86 17 12 7
BstZ17I GTA'TAC
Cuts at: 2243 2243
Size: 9421
Bsu36I CC'TnA_GG
Cuts at: 5149 5149
Size: 9421
BtgI C'CryG_G
Cuts at: 513 1065 2898 3021 3135 4113 513
Size: 552 1833 123 114 978 5821
Fragments arranged by size:
5821 1833 978 552 123 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2045 6681 487
Size: 683 56 819 4636 3227
Fragments arranged by size:
4636 3227 819 683 56
BtsI GCAGTG_nn'
Cuts at: 2913 3447 4582 5981 6937 7731 7751 2913
Size: 534 1135 1399 956 794 20 4583
Fragments arranged by size:
4583 1399 1135 956 794 534 20
ClaI AT'CG_AT
Cuts at: 1554 2235 6177 1554
Size: 681 3942 4798
Fragments arranged by size:
4798 3942 681
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9386
DraI TTT'AAA
Cuts at: 6725 6725
Size: 9421
DraIII CAC_nnn'GTG
Cuts at: 4618 5209 4618
Size: 591 8830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2185 3785 6428 8428 809
Size: 507 869 1600 2643 2000 1802
Fragments arranged by size:
2643 2000 1802 1600 869 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2857 2901 3411
Size: 54 1425 50 275 18 1027 44 510
Cuts at: 3411 5562 6868 7757 9386 8
Size: 2151 1306 889 1629 43
Fragments arranged by size:
2151 1629 1425 1306 1027 889 510 275
54 50 44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2857 2901 5562 6868 1537
Size: 275 1045 44 2661 1306 4090
Fragments arranged by size:
4090 2661 1306 1045 275 44
EarI CTCTTCn'nnn_
Cuts at: 1353 3671 3906 3930 3945 3969 5892 6012 6095
Size: 2318 235 24 15 24 1923 120 83
Cuts at: 6095 6100 6474 6498 6507 6522 7351 1353
Size: 5 374 24 9 15 829 3423
Fragments arranged by size:
3423 2318 1923 829 374 235 120 83
24 24 24 15 15 9 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5800 8008 8382 8528 387
Size: 1064 138 4211 2208 374 146 1280
Fragments arranged by size:
4211 2208 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2555 5483 2555
Size: 2928 6493
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2392 2442 2945 3038 3242 3320 3549
Size: 393 760 50 503 93 204 78 229
Cuts at: 3549 4175 4874 5331 5351 5393 5892 6371 6419
Size: 626 699 457 20 42 499 479 48
Cuts at: 6419 7484 8081 8868 9136 1239
Size: 1065 597 787 268 1524
Fragments arranged by size:
1524 1065 787 760 699 626 597 503
499 479 457 393 268 229 204 93
78 50 48 42 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2094 2313 6353 2094
Size: 219 4040 5162
Fragments arranged by size:
5162 4040 219
EcoO109I rG'GnC_Cy
Cuts at: 2045 2057 2324 2466 2467 2513 2916 4170 4195
Size: 12 267 142 1 46 403 1254 25
Cuts at: 4195 4647 4681 5925 2045
Size: 452 34 1244 5541
Fragments arranged by size:
5541 1254 1244 452 403 267 142 46
34 25 12 1
EcoRI G'AATT_C
Cuts at: 6730 7108 6730
Size: 378 9043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6047 6047
Size: 9421
FseI GG_CCGG'CC
Cuts at: 2863 2863
Size: 9421
FspI TGC'GCA
Cuts at: 3206 7927 3206
Size: 4721 4700
HaeII r_GCGC'y
Cuts at: 1678 3265 5938 8568 1678
Size: 1587 2673 2630 2531
Fragments arranged by size:
2673 2630 2531 1587
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4133 4165 4921 4953 5218
Size: 32 135 32 2561 32 756 32 265
Cuts at: 5218 5250 5609 5641 6116 6148 6307 6339 6396
Size: 32 359 32 475 32 159 32 57
Cuts at: 6396 6428 6521 6533 6553 6565 8062 8094 8136
Size: 32 93 12 20 12 1497 32 42
Cuts at: 8136 8168 1373
Size: 32 2626
Fragments arranged by size:
2626 2561 1497 756 475 359 265 159
135 93 57 42 32 32 32 32
32 32 32 32 32 32 32 20
12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2776 6765 7011 669
Size: 988 281 838 3989 246 3079
Fragments arranged by size:
3989 3079 988 838 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9421
HpaI GTT'AAC
Cuts at: 2776 7011 2776
Size: 4235 5186
KpnI G_GTAC'C
Cuts at: 6746 6746
Size: 9421
MfeI C'AATT_G
Cuts at: 7020 7020
Size: 9421
MluI A'CGCG_T
Cuts at: 9297 9297
Size: 9421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 8535 8719 1305
Size: 7230 184 2007
Fragments arranged by size:
7230 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7599
Fragments arranged by size:
7599 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 4550 5030 7379 7738 7897 518
Size: 648 1131 2253 480 2349 359 159 2042
Fragments arranged by size:
2349 2253 2042 1131 648 480 359 159
MspA1I CmG'CkG
Cuts at: 1606 2539 2900 2965 3178 3853 4654 5417 6122
Size: 933 361 65 213 675 801 763 705
Cuts at: 6122 6837 7514 8662 8907 1606
Size: 715 677 1148 245 2120
Fragments arranged by size:
2120 1148 933 801 763 715 705 677
675 361 245 213 65
NaeI GCC'GGC
Cuts at: 2766 2861 2934 2766
Size: 95 73 9253
Fragments arranged by size:
9253 95 73
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8869
NdeI CA'TA_TG
Cuts at: 387 4372 4944 387
Size: 3985 572 4864
Fragments arranged by size:
4864 3985 572
NgoMIV G'CCGG_C
Cuts at: 2764 2859 2932 2764
Size: 95 73 9253
Fragments arranged by size:
9253 95 73
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9421
NotI GC'GGCC_GC
Cuts at: 2901 5562 6868 2901
Size: 2661 1306 5454
Fragments arranged by size:
5454 2661 1306
NsiI A_TGCA'T
Cuts at: 2217 2226 2217
Size: 9 9412
NspI r_CATG'y
Cuts at: 1132 1229 2215 3205 3380 3458 4078 4915 5614
Size: 97 986 990 175 78 620 837 699
Cuts at: 5614 6779 8324 1132
Size: 1165 1545 2229
Fragments arranged by size:
2229 1545 1165 990 986 837 699 620
175 97 78
PasI CC'CwG_GG
Cuts at: 1348 2042 2072 3733 1348
Size: 694 30 1661 7036
Fragments arranged by size:
7036 1661 694 30
PciI A'CATG_T
Cuts at: 4911 8320 4911
Size: 3409 6012
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2084 2147 4198 1108
Size: 976 63 2051 6331
Fragments arranged by size:
6331 2051 976 63
PfoI T'CCnGG_A
Cuts at: 2095 2146 3987 5849 2095
Size: 51 1841 1862 5667
Fragments arranged by size:
5667 1862 1841 51
PmeI GTTT'AAAC
Cuts at: 6725 6725
Size: 9421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7490 7522 9003 9035 7490
Size: 32 1481 32 7876
Fragments arranged by size:
7876 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2513 4170 4647 5925 2513
Size: 1657 477 1278 6009
Fragments arranged by size:
6009 1657 1278 477
PshAI GACnn'nnGTC
Cuts at: 1613 4598 1613
Size: 2985 6436
PsiI TTA'TAA
Cuts at: 2571 4505 6991 2571
Size: 1934 2486 5001
Fragments arranged by size:
5001 2486 1934
PspOMI G'GGCC_C
Cuts at: 2323 2466 2915 4680 2323
Size: 143 449 1765 7064
Fragments arranged by size:
7064 1765 449 143
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5264 5296 5264
Size: 32 9389
PstI C_TGCA'G
Cuts at: 830 2613 2970 4659 4724 4926 5469 5658 6773
Size: 1783 357 1689 65 202 543 189 1115
Cuts at: 6773 830
Size: 3478
Fragments arranged by size:
3478 1783 1689 1115 543 357 202 189
65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9421
PvuII CAG'CTG
Cuts at: 2539 2965 3178 3853 5417 2539
Size: 426 213 675 1564 6543
Fragments arranged by size:
6543 1564 675 426 213
SacI G_AGCT'C
Cuts at: 2557 5485 2557
Size: 2928 6493
SacII CC_GC'GG
Cuts at: 2901 2901
Size: 9421
SalI G'TCGA_C
Cuts at: 1936 6763 1936
Size: 4827 4594
SanDI GG'GwC_CC
Cuts at: 4170 4647 5925 4170
Size: 477 1278 7666
Fragments arranged by size:
7666 1278 477
SbfI CC_TGCA'GG
Cuts at: 5658 6773 5658
Size: 1115 8306
ScaI AGT'ACT
Cuts at: 7669 9381 7669
Size: 1712 7709
SfcI C'TryA_G
Cuts at: 826 1045 2609 2966 4655 4720 4922 5465 5654
Size: 219 1564 357 1689 65 202 543 189
Cuts at: 5654 5950 6645 6769 7904 8585 8776 826
Size: 296 695 124 1135 681 191 1471
Fragments arranged by size:
1689 1564 1471 1135 695 681 543 357
296 219 202 191 189 124 65
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9421
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9421
SmaI CCC'GGG
Cuts at: 1872 2799 2914 2940 6748 1872
Size: 927 115 26 3808 4545
Fragments arranged by size:
4545 3808 927 115 26
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6032 7525 8426 8688 820
Size: 197 926 4089 1493 901 262 1553
Fragments arranged by size:
4089 1553 1493 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7212 493
Size: 6719 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9421
SphI G_CATG'C
Cuts at: 1132 2215 3205 3458 4078 6779 1132
Size: 1083 990 253 620 2701 3774
Fragments arranged by size:
3774 2701 1083 990 620 253
SrfI GCCC'GGGC
Cuts at: 2914 2914
Size: 9421
SspI AAT'ATT
Cuts at: 5 52 5913 7345 5
Size: 47 5861 1432 2081
Fragments arranged by size:
5861 2081 1432 47
StuI AGG'CCT
Cuts at: 1517 3012 1517
Size: 1495 7926
StyI C'CwwG_G
Cuts at: 513 1065 2356 4953 5101 6340 6860 513
Size: 552 1291 2597 148 1239 520 3074
Fragments arranged by size:
3074 2597 1291 1239 552 520 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2055 2328 2391 3305 3393 3617 3853
Size: 1255 475 273 63 914 88 224 236
Cuts at: 3853 5073 5493 7448 7465 7618 7803 8248 325
Size: 1220 420 1955 17 153 185 445 1498
Fragments arranged by size:
1955 1498 1255 1220 914 475 445 420
273 236 224 185 153 88 63 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4080 7667 9379
Size: 274 80 33 51 1443 2103 3587 1712
Cuts at: 9379 96
Size: 138
Fragments arranged by size:
3587 2103 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4990 5022 5960 5992 9381 9413 4990
Size: 32 938 32 3389 32 4998
Fragments arranged by size:
4998 3389 938 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9421
XbaI T'CTAG_A
Cuts at: 6757 6757
Size: 9421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3133 4460 3133
Size: 1327 8094
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9421
XmaI C'CCGG_G
Cuts at: 1870 2797 2912 2938 6746 1870
Size: 927 115 26 3808 4545
Fragments arranged by size:
4545 3808 927 115 26
XmnI GAAnn'nnTTC
Cuts at: 5336 6315 7550 5336
Size: 979 1235 7207
Fragments arranged by size:
7207 1235 979
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7229 276
Size: 53 83 186 936 5695 2468
Fragments arranged by size:
5695 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BbeI BsiWI EcoRV FspAI
KasI NarI NruI PacI PmlI PspXI RsrII SapI
SexAI SfiI SfoI SwaI