################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI AlwI AlwNI AocI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DpnII DraI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MboI MfeI MflI MluI MroI MseI MslI MunI MvnI NarI NcoI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Alw44I 327 GTGCAC G^TGCAC Aor51HI 224 AGCGCT AGC^GCT ApaLI 327 GTGCAC G^TGCAC AspHI 327 GTGCAC G[AT]GC[AT]^C BalI 255 TGGCCA TGG^CCA BbrPI 321 CACGTG CAC^GTG BglI 467 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 327 GTGCAC G[AGT]GC[ACT]^C BsaAI 321 CACGTG [CT]AC^GT[GA] BsgI 264 CTGCAC GTGCAG(16/14) BsiHKAI 327 GTGCAC G[AT]GC[AT]^C Bsp1286I 327 GTGCAC G[AGT]GC[ACT]^C BsrFI 520 GCCGGC [GA]^CCGG[CT] BsrI 393 CCAGT ACTGG(1/-1) BstEII 375 GGTGACC G^GT[ACGT]ACC BstF5I 129 GGATG GGATG(2/0) BstPI 375 GGTGACC G^GT[ACGT]ACC BstXI 353 CCAAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 520 GCCGGC [GA]^CCGG[CT] DraIII 321 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 224 AGCGCT AGC^GCT EcoO65I 375 GGTGACC G^GT[ACGT]ACC EcoT14I 525 CCTTGG C^C[AT][AT]GG FokI 129 GGATG GGATG(9/13) HgaI 237 GACGC GACGC(5/10) MaeII 322 ACGT A^CGT MaeIII 376 GTGAC ^GT[ACGT]AC MluNI 255 TGGCCA TGG^CCA MscI 255 TGGCCA TGG^CCA MspA1I 336 CAGCTG C[AC]G^C[GT]G NaeI 520 GCCGGC GCC^GGC NgoMIV 520 GCCGGC G^CCGGC PmaCI 321 CACGTG CAC^GTG PmlI 321 CACGTG CAC^GTG PvuII 336 CAGCTG CAG^CTG SmaI 470 CCCGGG CCC^GGG StuI 300 AGGCCT AGG^CCT StyI 525 CCTTGG C^C[AT][AT]GG Tsp45I 376 GTGAC ^GT[GC]AC XmaCI 470 CCCGGG C^CCGGG XmaI 470 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BcnI 470 CCCGG CC^[GC]GG 471 CCGGG BsaI 148 GAGACC GGTCTC(1/5) 546 GAGACC BseRI 142 GAGGAG GAGGAG(10/8) 145 GAGGAG BsmAI 148 GAGAC GTCTC(1/5) 546 GAGAC DraII 367 GGGACCC [GA]G^G[ACGT]CC[CT] 440 AGGACCC DsaI 96 CCACGG C^C[GA][CT]GG 282 CCACGG EaeI 46 CGGCCA [CT]^GGCC[GA] 255 TGGCCA EcoO109I 367 GGGACCC [GA]G^G[ACGT]CC[CT] 440 AGGACCC HaeII 6 GGCGCT [GA]GCGC^[CT] 224 AGCGCT HapII 471 CCGG C^CGG 521 CCGG HinfI 295 GAGTC G^A[ACGT]TC 434 GAGTC HpaII 471 CCGG C^CGG 521 CCGG HphI 375 GGTGA GGTGA(8/7) 350 TCACC MspI 471 CCGG C^CGG 521 CCGG NciI 470 CCCGG CC^[GC]GG 471 CCGGG NlaIII 253 CATG CATG^ 381 CATG PleI 295 GAGTC GAGTC(4/5) 434 GAGTC PpuMI 367 GGGACCC [GA]G^G[AT]CC[CT] 440 AGGACCC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AluI AvaI AvaII BbvI BsaJI BsiYI BslI BsmFI BsoBI BspMI BsrDI BssKI BstNI Cac8I CfoI Cfr13I EarI EcoRII Fnu4HI HaeIII HhaI HinP1I ItaI Ksp632I MboII MnlI MvaI MwoI NlaIV Sau96I ScrFI TseI