Restriction map for
pFN21AE9408
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HinfI HpaI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfcI SfuI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tsp509I TthHB8I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 343 GGCGCC G[GA]^CG[CT]C
AspI 970 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 449 TGGCCA TGG^CCA
BbeI 343 GGCGCC GGCGC^C
BbrPI 838 CACGTG CAC^GTG
BbsI 229 GTCTTC GAAGAC(2/6)
BbvCI 852 CCTCAGC CCTCAGC(-5/-2)
BglI 929 GCCTCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 229 GTCTTC GAAGAC(2/6)
BsaAI 838 CACGTG [CT]AC^GT[GA]
BsaHI 343 GGCGCC G[GA]^CG[CT]C
BseRI 127 GAGGAG GAGGAG(10/8)
BsmAI 12 GTCTC GTCTC(1/5)
BspMI 424 ACCTGC ACCTGC(4/8)
BstAPI 720 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 288 GGTGACC G^GT[ACGT]ACC
BstPI 288 GGTGACC G^GT[ACGT]ACC
DpnII 295 GATC ^GATC
DrdI 877 GACCCCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 788 CCGTGG C^C[GA][CT]GG
EcoO65I 288 GGTGACC G^GT[ACGT]ACC
EcoT14I 995 CCAAGG C^C[AT][AT]GG
Hin1I 343 GGCGCC G[GA]^CG[CT]C
KasI 343 GGCGCC G^GCGCC
MboI 295 GATC ^GATC
MluNI 449 TGGCCA TGG^CCA
MscI 449 TGGCCA TGG^CCA
NarI 343 GGCGCC GG^CGCC
NdeII 295 GATC ^GATC
PflFI 970 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1047 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 838 CACGTG CAC^GTG
PmlI 838 CACGTG CAC^GTG
PvuII 867 CAGCTG CAG^CTG
SacI 990 GAGCTC GAGCT^C
Sau3AI 295 GATC ^GATC
SexAI 281 ACCTGGT A^CC[AT]GGT
SfiI 928 GGCCTCTTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 343 GGCGCC GGC^GCC
SgrAI 531 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 245 CCCGGG CCC^GGG
SphI 980 GCATGC GCATG^C
StyI 995 CCAAGG C^C[AT][AT]GG
Tth111I 970 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1047 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 275 CCATCAACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 245 CCCGGG C^CCGGG
XmaI 245 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 832 GTCTAC GT^[AC][GT]AC
886 GTCTAC
AfaI 225 GTAC GT^AC
336 GTAC
Alw44I 175 GTGCAC G^TGCAC
662 GTGCAC
ApaI 22 GGGCCC GGGCC^C
1063 GGGCCC
ApaLI 175 GTGCAC G^TGCAC
662 GTGCAC
AvaI 245 CCCGGG C^[CT]CG[GA]G
1066 CCCGAG
BsmFI 40 GTCCC GGGAC(10/14)
312 GTCCC
BsoBI 245 CCCGGG C^[CT]CG[GA]G
1066 CCCGAG
BsrI 300 ACTGG ACTGG(1/-1)
902 CCAGT
BssSI 819 CACGAG CACGAG(-5/-1)
826 CTCGTG
BstF5I 411 CATCC GGATG(2/0)
1017 CATCC
EarI 415 CTCTTC CTCTTC(1/4)
931 CTCTTC
EcoNI 494 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1041 CCTCACCCAGG
FokI 411 CATCC GGATG(9/13)
1017 CATCC
Ksp632I 415 CTCTTC CTCTTC(1/4)
931 CTCTTC
NaeI 572 GCCGGC GCC^GGC
1027 GCCGGC
NgoMIV 572 GCCGGC G^CCGGC
1027 GCCGGC
RsaI 225 GTAC GT^AC
336 GTAC
SfaNI 428 GCATC GCATC(5/9)
653 GCATC
StuI 150 AGGCCT AGG^CCT
927 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiEI BsiHKAI
BsiYI BslI Bsp1286I BsrFI BssKI
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EaeI
EagI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV NspI Sau96I
ScrFI TseI Tsp45I TspRI