(Circular) (Six-base) MAPSORT of: pFN21AE9408.seq Check: 8238 from: 1 to: 5779 pFN21AE9408 5779 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2438 2438 Size: 5779 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3589 278 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2838 2892 3122 1937 Size: 901 54 230 4594 Fragments arranged by size: 4594 901 230 54 Acc65I G'GTAC_C Cuts at: 3100 3100 Size: 5779 AclI AA'CG_TT Cuts at: 1563 3906 4279 1563 Size: 2343 373 3063 Fragments arranged by size: 3063 2343 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2490 3842 5226 5494 1632 Size: 858 1352 1384 268 1917 Fragments arranged by size: 1917 1384 1352 858 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5582 AflIII A'CryG_T Cuts at: 1203 4678 5655 1203 Size: 3475 977 1327 Fragments arranged by size: 3475 1327 977 AgeI A'CCGG_T Cuts at: 4610 4610 Size: 5779 AhdI GACnn_n'nnGTC Cuts at: 4508 4508 Size: 5779 AleI CACnn'nnGTG Cuts at: 1166 2060 1166 Size: 894 4885 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5779 AlwNI CAG_nnn'CTG Cuts at: 2358 2731 3058 3522 5094 2358 Size: 373 327 464 1572 3043 Fragments arranged by size: 3043 1572 464 373 327 ApaI G_GGCC'C Cuts at: 2031 3072 2031 Size: 1041 4738 ApaLI G'TGCA_C Cuts at: 2180 2667 3836 4992 2180 Size: 487 1169 1156 2967 Fragments arranged by size: 2967 1169 1156 487 ApoI r'AATT_y Cuts at: 1493 3088 3314 3466 5575 1493 Size: 1595 226 152 2109 1697 Fragments arranged by size: 2109 1697 1595 226 152 AseI AT'TA_AT Cuts at: 160 1032 4333 160 Size: 872 3301 1606 Fragments arranged by size: 3301 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 AvaI C'yCGr_G Cuts at: 1870 1943 2250 3071 3104 1870 Size: 73 307 821 33 4545 Fragments arranged by size: 4545 821 307 73 33 BamHI G'GATC_C Cuts at: 3109 3476 3109 Size: 367 5412 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2098 2144 2348 2540 3100 Size: 325 217 627 311 46 204 192 560 Cuts at: 3100 4555 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 560 325 311 217 204 192 46 BanII G_rGCy'C Cuts at: 1391 2031 2964 2999 3008 3072 3098 1391 Size: 640 933 35 9 64 26 4072 Fragments arranged by size: 4072 933 640 64 35 26 9 BbeI G_GCGC'C Cuts at: 2352 2352 Size: 5779 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2227 928 Size: 971 328 4480 Fragments arranged by size: 4480 971 328 BbvCI CC'TCA_GC Cuts at: 2858 2858 Size: 5779 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3970 4004 1903 Size: 34 50 34 1949 34 3678 Fragments arranged by size: 3678 1949 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3671 4881 3671 Size: 1210 4569 BclI T'GATC_A Cuts at: 1548 1548 Size: 5779 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2940 4390 136 Size: 107 122 71 631 605 1268 1450 1525 Fragments arranged by size: 1525 1450 1268 631 605 122 107 71 BglII A'GATC_T Cuts at: 5774 5774 Size: 5779 BlpI GC'TnA_GC Cuts at: 3196 3196 Size: 5779 Bme1580I G_kGCm'C Cuts at: 1790 2031 2103 2184 2671 3072 3840 4996 1790 Size: 241 72 81 487 401 768 1156 2573 Fragments arranged by size: 2573 1156 768 487 401 241 81 72 BmgBI CAC'GTC Cuts at: 1318 2383 2977 1318 Size: 1065 594 4120 Fragments arranged by size: 4120 1065 594 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3510 4468 442 Size: 416 53 83 399 24 2093 958 1753 Fragments arranged by size: 2093 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2004 2114 2633 4439 1239 Size: 765 110 519 1806 2579 Fragments arranged by size: 2579 1806 765 519 110 Bpu10I CC'TnA_GC Cuts at: 2858 3007 2858 Size: 149 5630 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3904 4769 5067 3904 Size: 865 298 4616 Fragments arranged by size: 4616 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4442 882 Size: 617 2943 2219 Fragments arranged by size: 2943 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2845 3570 493 Size: 711 1641 725 2702 Fragments arranged by size: 2702 1641 725 711 BsaBI GATnn'nnATC Cuts at: 5773 5773 Size: 5779 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2349 3586 3968 4603 Size: 53 83 186 936 816 1237 382 635 Cuts at: 4603 275 Size: 1451 Fragments arranged by size: 1451 1237 936 816 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4212 4610 4884 5031 5442 4212 Size: 398 274 147 411 4549 Fragments arranged by size: 4549 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2981 3011 1178 Size: 30 165 30 1578 30 3946 Fragments arranged by size: 3946 1578 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 1186 Size: 961 4818 BseYI C'CCAG_C Cuts at: 2560 2963 4982 5478 2560 Size: 403 2019 496 2861 Fragments arranged by size: 2861 2019 496 403 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5206 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 2656 2681 3229 3990 5018 Size: 275 186 36 619 25 548 761 1028 Cuts at: 5018 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 619 548 275 186 36 25 BsiHKAI G_wGCw'C Cuts at: 2184 2214 2671 2745 2999 3840 3925 4996 2184 Size: 30 457 74 254 841 85 1071 2967 Fragments arranged by size: 2967 1071 841 457 254 85 74 30 BsmI GAATG_Cn' Cuts at: 3290 3383 3290 Size: 93 5686 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2103 2184 2214 2671 2745 2964 Size: 399 241 72 81 30 457 74 219 Cuts at: 2964 2999 3008 3072 3098 3840 3925 4996 1391 Size: 35 9 64 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 457 399 241 219 85 81 74 72 64 35 30 26 9 BspEI T'CCGG_A Cuts at: 5442 5442 Size: 5779 BspHI T'CATG_A Cuts at: 3668 5398 3668 Size: 1730 4049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2438 3134 844 Size: 1594 696 3489 Fragments arranged by size: 3489 1594 696 BsrBI CCG'CTC Cuts at: 1394 1823 3666 1394 Size: 429 1843 3507 Fragments arranged by size: 3507 1843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4274 4448 65 Size: 1183 3026 174 1396 Fragments arranged by size: 3026 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2537 2577 2651 3032 4423 4610 1700 Size: 110 727 40 74 381 1391 187 2869 Fragments arranged by size: 2869 1391 727 381 187 110 74 40 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5779 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5779 BssSI C'ACGA_G Cuts at: 2824 2831 3839 4851 2824 Size: 7 1008 1012 3752 Fragments arranged by size: 3752 1012 1008 7 BstAPI GCAn_nnn'nTGC Cuts at: 794 2731 794 Size: 1937 3842 BstBI TT'CG_AA Cuts at: 4583 4583 Size: 5779 BstEII G'GTnAC_C Cuts at: 1184 2293 1184 Size: 1109 4670 BstXI CCAn_nnnn'nTGG Cuts at: 2092 2160 2197 2287 2092 Size: 68 37 90 5584 Fragments arranged by size: 5584 90 68 37 BstYI r'GATC_y Cuts at: 1972 3109 3476 3861 3878 5330 5416 5428 5774 Size: 1137 367 385 17 1452 86 12 346 Cuts at: 5774 1972 Size: 1977 Fragments arranged by size: 1977 1452 1137 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2793 513 Size: 552 1728 3499 Fragments arranged by size: 3499 1728 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5040 Fragments arranged by size: 5040 683 56 BtsI GCAGTG_nn' Cuts at: 2067 2407 3295 4089 4109 2067 Size: 340 888 794 20 3737 Fragments arranged by size: 3737 888 794 340 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5779 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5744 DraI TTT'AAA Cuts at: 3083 3083 Size: 5779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2888 4786 809 Size: 507 1572 1898 1802 Fragments arranged by size: 1898 1802 1572 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2454 2532 Size: 54 1425 50 275 18 204 420 78 Cuts at: 2532 2653 2678 2790 2847 2941 3226 4115 5744 Size: 121 25 112 57 94 285 889 1629 Cuts at: 5744 8 Size: 43 Fragments arranged by size: 1629 1425 889 420 285 275 204 121 112 94 78 57 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2034 2653 2678 3226 1537 Size: 275 222 619 25 548 4090 Fragments arranged by size: 4090 619 548 275 222 25 EarI CTCTTCn'nnn_ Cuts at: 1353 2426 2942 3709 1353 Size: 1073 516 767 3423 Fragments arranged by size: 3423 1073 767 516 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2681 4366 4740 4886 387 Size: 1064 138 1092 1685 374 146 1280 Fragments arranged by size: 1685 1280 1092 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2997 2997 Size: 5779 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2004 2114 2490 2633 3842 4439 5226 Size: 393 372 110 376 143 1209 597 787 Cuts at: 5226 5494 1239 Size: 268 1524 Fragments arranged by size: 1524 1209 787 597 393 376 372 268 143 110 EcoNI CCTnn'n_nnAGG Cuts at: 2503 3050 2503 Size: 547 5232 EcoO109I rG'GnC_Cy Cuts at: 2027 2028 3055 2027 Size: 1 1027 4751 Fragments arranged by size: 4751 1027 1 EcoRI G'AATT_C Cuts at: 3088 3466 3088 Size: 378 5401 FspI TGC'GCA Cuts at: 4285 4285 Size: 5779 HaeII r_GCGC'y Cuts at: 1678 2171 2228 2352 2710 2801 4926 1678 Size: 493 57 124 358 91 2125 2531 Fragments arranged by size: 2531 2125 493 358 124 91 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4420 4452 4494 4526 1373 Size: 32 135 32 2848 32 42 32 2626 Fragments arranged by size: 2848 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3123 3369 669 Size: 988 281 1185 246 3079 Fragments arranged by size: 3079 1185 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5779 HpaI GTT'AAC Cuts at: 3369 3369 Size: 5779 KasI G'GCGC_C Cuts at: 2348 2348 Size: 5779 KpnI G_GTAC'C Cuts at: 3104 3104 Size: 5779 MfeI C'AATT_G Cuts at: 3378 3378 Size: 5779 MluI A'CGCG_T Cuts at: 5655 5655 Size: 5779 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4893 5077 1305 Size: 3588 184 2007 Fragments arranged by size: 3588 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2456 10 Size: 54 1425 343 624 3333 Fragments arranged by size: 3333 1425 624 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2060 2438 2642 3737 4096 4255 518 Size: 648 894 378 204 1095 359 159 2042 Fragments arranged by size: 2042 1095 894 648 378 359 204 159 MspA1I CmG'CkG Cuts at: 1606 2024 2088 2484 2874 2970 3195 3872 5020 Size: 418 64 396 390 96 225 677 1148 Cuts at: 5020 5265 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 418 396 390 245 225 96 64 NaeI GCC'GGC Cuts at: 2579 3034 2579 Size: 455 5324 NarI GG'CG_CC Cuts at: 2349 2349 Size: 5779 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5227 NdeI CA'TA_TG Cuts at: 387 387 Size: 5779 NgoMIV G'CCGG_C Cuts at: 2577 3032 2577 Size: 455 5324 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5779 NotI GC'GGCC_GC Cuts at: 3226 3226 Size: 5779 NspI r_CATG'y Cuts at: 1132 1229 2407 2815 2989 3137 4682 1132 Size: 97 1178 408 174 148 1545 2229 Fragments arranged by size: 2229 1545 1178 408 174 148 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5779 PciI A'CATG_T Cuts at: 4678 4678 Size: 5779 PflMI CCAn_nnn'nTGG Cuts at: 1108 3058 1108 Size: 1950 3829 PfoI T'CCnGG_A Cuts at: 2610 2610 Size: 5779 PmeI GTTT'AAAC Cuts at: 3083 3083 Size: 5779 PmlI CAC'GTG Cuts at: 2845 2845 Size: 5779 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3848 3880 5361 5393 3848 Size: 32 1481 32 4234 Fragments arranged by size: 4234 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5779 PsiI TTA'TAA Cuts at: 3349 3349 Size: 5779 PspOMI G'GGCC_C Cuts at: 2027 3068 2027 Size: 1041 4738 PstI C_TGCA'G Cuts at: 830 3131 830 Size: 2301 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5779 PvuII CAG'CTG Cuts at: 2874 2874 Size: 5779 SacI G_AGCT'C Cuts at: 2999 2999 Size: 5779 SalI G'TCGA_C Cuts at: 1936 3121 1936 Size: 1185 4594 SbfI CC_TGCA'GG Cuts at: 3131 3131 Size: 5779 ScaI AGT'ACT Cuts at: 4027 5739 4027 Size: 1712 4067 SexAI A'CCwGG_T Cuts at: 2286 2286 Size: 5779 SfcI C'TryA_G Cuts at: 826 1045 3127 4262 4943 5134 826 Size: 219 2082 1135 681 191 1471 Fragments arranged by size: 2082 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2940 2940 Size: 5779 SfoI GGC'GCC Cuts at: 2350 2350 Size: 5779 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 SgrAI Cr'CCGG_yG Cuts at: 1700 2537 1700 Size: 837 4942 SmaI CCC'GGG Cuts at: 1872 2252 3106 1872 Size: 380 854 4545 Fragments arranged by size: 4545 854 380 SmlI C'TyrA_G Cuts at: 820 1017 1943 3883 4784 5046 820 Size: 197 926 1940 901 262 1553 Fragments arranged by size: 1940 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3570 493 Size: 3077 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5779 SphI G_CATG'C Cuts at: 1132 2989 3137 1132 Size: 1857 148 3774 Fragments arranged by size: 3774 1857 148 SspI AAT'ATT Cuts at: 5 52 3703 5 Size: 47 3651 2081 Fragments arranged by size: 3651 2081 47 StuI AGG'CCT Cuts at: 1517 2157 2934 1517 Size: 640 777 4362 Fragments arranged by size: 4362 777 640 StyI C'CwwG_G Cuts at: 513 1065 3000 3218 513 Size: 552 1935 218 3074 Fragments arranged by size: 3074 1935 552 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2312 3065 3806 3823 3976 4161 4606 Size: 1255 732 753 741 17 153 185 445 Cuts at: 4606 325 Size: 1498 Fragments arranged by size: 1498 1255 753 741 732 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2340 4025 5737 Size: 274 80 33 51 1443 363 1685 1712 Cuts at: 5737 96 Size: 138 Fragments arranged by size: 1712 1685 1443 363 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2981 3013 5739 5771 2981 Size: 32 2726 32 2989 Fragments arranged by size: 2989 2726 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2978 1316 Size: 1662 4117 XbaI T'CTAG_A Cuts at: 3115 3115 Size: 5779 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2287 2287 Size: 5779 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5779 XmaI C'CCGG_G Cuts at: 1870 2250 3104 1870 Size: 380 854 4545 Fragments arranged by size: 4545 854 380 XmnI GAAnn'nnTTC Cuts at: 3908 3908 Size: 5779 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3587 276 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SrfI SwaI