Restriction map for
pFN21AE9408
(Linear) (Six-base) MAPSORT of: KIEE9408.seq Check: 1775 from: 1 to: 1074
KIEE9408.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 433 1074
Size: 433 641
AccI GT'mk_AC
Cuts at: 0 833 887 1074
Size: 833 54 187
Fragments arranged by size:
833 187 54
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1074
Size: 485 589
AleI CACnn'nnGTG
Cuts at: 0 55 1074
Size: 55 1019
AlwNI CAG_nnn'CTG
Cuts at: 0 353 726 1053 1074
Size: 353 373 327 21
Fragments arranged by size:
373 353 327 21
ApaI G_GGCC'C
Cuts at: 0 26 1067 1074
Size: 26 1041 7
Fragments arranged by size:
1041 26 7
ApaLI G'TGCA_C
Cuts at: 0 175 662 1074
Size: 175 487 412
Fragments arranged by size:
487 412 175
AvaI C'yCGr_G
Cuts at: 0 245 1066 1074
Size: 245 821 8
Fragments arranged by size:
821 245 8
BanI G'GyrC_C
Cuts at: 0 93 139 343 535 1074
Size: 93 46 204 192 539
Fragments arranged by size:
539 204 192 93 46
BanII G_rGCy'C
Cuts at: 0 26 959 994 1003 1067 1074
Size: 26 933 35 9 64 7
Fragments arranged by size:
933 64 35 26 9 7
BbeI G_GCGC'C
Cuts at: 0 347 1074
Size: 347 727
BbsI GAAGACnn'nnnn_
Cuts at: 0 222 1074
Size: 222 852
BbvCI CC'TCA_GC
Cuts at: 0 853 1074
Size: 853 221
BglI GCCn_nnn'nGGC
Cuts at: 0 935 1074
Size: 935 139
Bme1580I G_kGCm'C
Cuts at: 0 26 98 179 666 1067 1074
Size: 26 72 81 487 401 7
Fragments arranged by size:
487 401 81 72 26 7
BmgBI CAC'GTC
Cuts at: 0 378 972 1074
Size: 378 594 102
Fragments arranged by size:
594 378 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 628 1074
Size: 109 519 446
Fragments arranged by size:
519 446 109
Bpu10I CC'TnA_GC
Cuts at: 0 853 1002 1074
Size: 853 149 72
Fragments arranged by size:
853 149 72
BsaAI yAC'GTr
Cuts at: 0 840 1074
Size: 840 234
BsaHI Gr'CG_yC
Cuts at: 0 344 1074
Size: 344 730
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 976 1006 1074
Size: 976 30 68
Fragments arranged by size:
976 68 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 1074
Size: 142 932
BseYI C'CCAG_C
Cuts at: 0 555 958 1074
Size: 555 403 116
Fragments arranged by size:
555 403 116
BsiEI CG_ry'CG
Cuts at: 0 32 651 676 1074
Size: 32 619 25 398
Fragments arranged by size:
619 398 32 25
BsiHKAI G_wGCw'C
Cuts at: 0 179 209 666 740 994 1074
Size: 179 30 457 74 254 80
Fragments arranged by size:
457 254 179 80 74 30
Bsp1286I G_dGCh'C
Cuts at: 0 26 98 179 209 666 740 959 994
Size: 26 72 81 30 457 74 219 35
Cuts at: 994 1003 1067 1074
Size: 9 64 7
Fragments arranged by size:
457 219 81 74 72 64 35 30
26 9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 433 1074
Size: 433 641
BsrFI r'CCGG_y
Cuts at: 0 532 572 646 1027 1074
Size: 532 40 74 381 47
Fragments arranged by size:
532 381 74 47 40
BssSI C'ACGA_G
Cuts at: 0 819 826 1074
Size: 819 7 248
Fragments arranged by size:
819 248 7
BstAPI GCAn_nnn'nTGC
Cuts at: 0 726 1074
Size: 726 348
BstEII G'GTnAC_C
Cuts at: 0 288 1074
Size: 288 786
BstXI CCAn_nnnn'nTGG
Cuts at: 0 87 155 192 282 1074
Size: 87 68 37 90 792
Fragments arranged by size:
792 90 87 68 37
BtgI C'CryG_G
Cuts at: 0 788 1074
Size: 788 286
BtsI GCAGTG_nn'
Cuts at: 0 62 402 1074
Size: 62 340 672
Fragments arranged by size:
672 340 62
DrdI GACnn_nn'nnGTC
Cuts at: 0 883 1074
Size: 883 191
EaeI y'GGCC_r
Cuts at: 0 29 449 527 648 673 785 842 936
Size: 29 420 78 121 25 112 57 94
Cuts at: 936 1074
Size: 138
Fragments arranged by size:
420 138 121 112 94 78 57 29
25
EagI C'GGCC_G
Cuts at: 0 29 648 673 1074
Size: 29 619 25 401
Fragments arranged by size:
619 401 29 25
EarI CTCTTCn'nnn_
Cuts at: 0 421 937 1074
Size: 421 516 137
Fragments arranged by size:
516 421 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 676 1074
Size: 676 398
EcoICRI GAG'CTC
Cuts at: 0 992 1074
Size: 992 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 485 628 1074
Size: 109 376 143 446
Fragments arranged by size:
446 376 143 109
EcoNI CCTnn'n_nnAGG
Cuts at: 0 498 1045 1074
Size: 498 547 29
Fragments arranged by size:
547 498 29
EcoO109I rG'GnC_Cy
Cuts at: 0 22 23 1050 1074
Size: 22 1 1027 24
Fragments arranged by size:
1027 24 22 1
HaeII r_GCGC'y
Cuts at: 0 166 223 347 705 796 1074
Size: 166 57 124 358 91 278
Fragments arranged by size:
358 278 166 124 91 57
KasI G'GCGC_C
Cuts at: 0 343 1074
Size: 343 731
MscI TGG'CCA
Cuts at: 0 451 1074
Size: 451 623
MslI CAynn'nnrTG
Cuts at: 0 55 433 637 1074
Size: 55 378 204 437
Fragments arranged by size:
437 378 204 55
MspA1I CmG'CkG
Cuts at: 0 19 83 479 869 965 1074
Size: 19 64 396 390 96 109
Fragments arranged by size:
396 390 109 96 64 19
NaeI GCC'GGC
Cuts at: 0 574 1029 1074
Size: 574 455 45
Fragments arranged by size:
574 455 45
NarI GG'CG_CC
Cuts at: 0 344 1074
Size: 344 730
NgoMIV G'CCGG_C
Cuts at: 0 572 1027 1074
Size: 572 455 47
Fragments arranged by size:
572 455 47
NspI r_CATG'y
Cuts at: 0 402 810 984 1074
Size: 402 408 174 90
Fragments arranged by size:
408 402 174 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 1053 1074
Size: 1053 21
PfoI T'CCnGG_A
Cuts at: 0 605 1074
Size: 605 469
PmlI CAC'GTG
Cuts at: 0 840 1074
Size: 840 234
PspOMI G'GGCC_C
Cuts at: 0 22 1063 1074
Size: 22 1041 11
Fragments arranged by size:
1041 22 11
PvuII CAG'CTG
Cuts at: 0 869 1074
Size: 869 205
SacI G_AGCT'C
Cuts at: 0 994 1074
Size: 994 80
SexAI A'CCwGG_T
Cuts at: 0 281 1074
Size: 281 793
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 935 1074
Size: 935 139
SfoI GGC'GCC
Cuts at: 0 345 1074
Size: 345 729
SgrAI Cr'CCGG_yG
Cuts at: 0 532 1074
Size: 532 542
SmaI CCC'GGG
Cuts at: 0 247 1074
Size: 247 827
SphI G_CATG'C
Cuts at: 0 984 1074
Size: 984 90
StuI AGG'CCT
Cuts at: 0 152 929 1074
Size: 152 777 145
Fragments arranged by size:
777 152 145
StyI C'CwwG_G
Cuts at: 0 995 1074
Size: 995 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 307 1060 1074
Size: 307 753 14
Fragments arranged by size:
753 307 14
TatI w'GTAC_w
Cuts at: 0 335 1074
Size: 335 739
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 976 1008 1074
Size: 976 32 66
Fragments arranged by size:
976 66 32
Tth111I GACn'n_nGTC
Cuts at: 0 973 1074
Size: 973 101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 1074
Size: 282 792
XmaI C'CCGG_G
Cuts at: 0 245 1074
Size: 245 829
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BbvCI BglI Bme1580I BmgBI
BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BssSI BstAPI BstEII BstXI
BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII KasI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PflMI PfoI PmlI PspOMI
PvuII SacI SexAI SfiI SfoI SgrAI SmaI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BplI BpuEI BsaI BsaBI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SmlI SnaBI SpeI SrfI SspI SwaI
XbaI XhoI XmnI ZraI