################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AvaII AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaII KasI KpnI KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 1267 GTCTAC GT^[AC][GT]AC AfaI 351 GTAC GT^AC AlwNI 815 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 571 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 233 GAGCTC G[AT]GC[AT]^C BamHI 1039 GGATCC G^GATCC BanII 233 GAGCTC G[GA]GC[CT]^C BfaI 485 CTAG C^TAG BpmI 530 CTGGAG CTGGAG(16/14) BsaAI 1270 TACGTG [CT]AC^GT[GA] BsaI 933 GAGACC GGTCTC(1/5) BsgI 83 GTGCAG GTGCAG(16/14) BsiHKAI 233 GAGCTC G[AT]GC[AT]^C BsmI 582 GAATGC GAATGC(1/-1) BssSI 449 CTCGTG CACGAG(-5/-1) BstYI 1039 GGATCC [GA]^GATC[CT] Cfr13I 960 GGGCC G^G[ACGT]CC DsaI 269 CCACGG C^C[GA][CT]GG EcoRI 437 GAATTC G^AATTC EcoRV 607 GATATC GAT^ATC EcoT14I 636 CCAAGG C^C[AT][AT]GG EcoT22I 720 ATGCAT ATGCA^T FauI 659 GCGGG CCCGC(4/6) HincII 766 GTTAAC GT[CT]^[GA]AC HindII 766 GTTAAC GT[CT]^[GA]AC HindIII 216 AAGCTT A^AGCTT HpaI 766 GTTAAC GTT^AAC HphI 615 TCACC GGTGA(8/7) MaeI 485 CTAG C^TAG MflI 1039 GGATCC [GA]^GATC[CT] NheI 484 GCTAGC G^CTAGC NsiI 720 ATGCAT ATGCA^T NspI 664 GCATGC [GA]CATG^[CT] PleI 140 GAGTC GAGTC(4/5) PstI 200 CTGCAG CTGCA^G RsaI 351 GTAC GT^AC SacI 233 GAGCTC GAGCT^C Sau96I 960 GGGCC G^G[ACGT]CC SphI 664 GCATGC GCATG^C SspI 1381 AATATT AAT^ATT StyI 636 CCAAGG C^C[AT][AT]GG SwaI 921 ATTTAAAT ATTT^AAAT TfiI 285 GAATC G^A[AT]TC XcmI 104 CCAAAGGTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1039 GGATCC [GA]^GATC[CT] XmnI 571 GAACGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwI 1040 GATCC GGATC(4/5) 1039 GGATC AseI 70 ATTAAT AT^TAAT 1119 ATTAAT AsnI 70 ATTAAT AT^TAAT 1119 ATTAAT BglI 169 GCCATACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 953 GCCAAGAGGGC BmyI 51 GGGCAC G[AGT]GC[ACT]^C 233 GAGCTC BsiYI 223 CCATGCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 419 CCATGTTGTGG BslI 223 CCATGCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 419 CCATGTTGTGG BsmFI 99 GTCCC GGGAC(10/14) 385 GTCCC Bsp1286I 51 GGGCAC G[AGT]GC[ACT]^C 233 GAGCTC BssKI 797 CCAGG ^CC[ACGT]GG 1149 CCAGG BstF5I 296 CATCC GGATG(2/0) 357 CATCC BstNI 797 CCAGG CC^[AT]GG 1149 CCAGG DpnII 841 GATC ^GATC 1040 GATC DraI 922 TTTAAA TTT^AAA 1324 TTTAAA EarI 810 CTCTTC CTCTTC(1/4) 999 CTCTTC EcoRII 797 CCAGG ^CC[AT]GG 1149 CCAGG FokI 296 CATCC GGATG(9/13) 357 CATCC HinfI 140 GAGTC G^A[ACGT]TC 285 GAATC Ksp632I 810 CTCTTC CTCTTC(1/4) 999 CTCTTC MboI 841 GATC ^GATC 1040 GATC MvaI 797 CCAGG CC^[AT]GG 1149 CCAGG NdeII 841 GATC ^GATC 1040 GATC PflMI 223 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 419 CCATGTTGTGG PshBI 70 ATTAAT AT^TAAT 1119 ATTAAT Sau3AI 841 GATC ^GATC 1040 GATC ScrFI 797 CCAGG CC^[ACGT]GG 1149 CCAGG SmlI 25 CTCAAG C^T[CT][GA]AG 250 CTCAAG TspRI 228 CAGTG CA[GC]TG[ACGT][ACGT]^ 491 CAGTG Van91I 223 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 419 CCATGTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AcsI AluI ApoI BalI BbvI BsaJI BsmAI BsrI Cac8I DdeI EaeI Fnu4HI HaeIII ItaI MaeII MaeIII MboII MluNI MnlI MscI MseI MslI MspA1I MwoI NlaIII NlaIV PvuII SfaNI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TthHB8I