(Circular) (Six-base) MAPSORT of: pFN21AE6474.seq Check: 8492 from: 1 to: 6115 pFN21AE6474 6115 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3925 278 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3273 3458 1937 Size: 1336 185 4594 Fragments arranged by size: 4594 1336 185 Acc65I G'GTAC_C Cuts at: 3436 3436 Size: 6115 AclI AA'CG_TT Cuts at: 1563 4242 4615 1563 Size: 2679 373 3063 Fragments arranged by size: 3063 2679 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2465 2717 2802 4178 5562 5830 1632 Size: 833 252 85 1376 1384 268 1917 Fragments arranged by size: 1917 1384 1376 833 268 252 85 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5918 AflIII A'CryG_T Cuts at: 1203 5014 5991 1203 Size: 3811 977 1327 Fragments arranged by size: 3811 1327 977 AgeI A'CCGG_T Cuts at: 4946 4946 Size: 6115 AhdI GACnn_n'nnGTC Cuts at: 4844 4844 Size: 6115 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6115 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2146 1184 Size: 962 5153 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2701 2733 2701 Size: 32 6083 AlwNI CAG_nnn'CTG Cuts at: 2825 3858 5430 2825 Size: 1033 1572 3510 Fragments arranged by size: 3510 1572 1033 ApaLI G'TGCA_C Cuts at: 4172 5328 4172 Size: 1156 4959 ApoI r'AATT_y Cuts at: 1493 2094 2442 2808 3424 3650 3802 5911 1493 Size: 601 348 366 616 226 152 2109 1697 Fragments arranged by size: 2109 1697 616 601 366 348 226 152 AseI AT'TA_AT Cuts at: 160 1032 2076 3125 4669 160 Size: 872 1044 1049 1544 1606 Fragments arranged by size: 1606 1544 1049 1044 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 AvaI C'yCGr_G Cuts at: 1870 1943 3440 1870 Size: 73 1497 4545 Fragments arranged by size: 4545 1497 73 BamHI G'GATC_C Cuts at: 3044 3445 3812 3044 Size: 401 367 5347 Fragments arranged by size: 5347 401 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3436 4891 618 Size: 325 217 627 1649 1455 1842 Fragments arranged by size: 1842 1649 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2242 3434 1391 Size: 851 1192 4072 Fragments arranged by size: 4072 1192 851 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5144 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4306 4340 1903 Size: 34 2369 34 3678 Fragments arranged by size: 3678 2369 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4007 5217 4007 Size: 1210 4905 BclI T'GATC_A Cuts at: 1548 1548 Size: 6115 BfrBI ATG'CAT Cuts at: 2727 2727 Size: 6115 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2180 2964 4726 Size: 107 122 71 631 605 508 784 1762 Cuts at: 4726 136 Size: 1525 Fragments arranged by size: 1762 1525 784 631 605 508 122 107 71 BglII A'GATC_T Cuts at: 6110 6110 Size: 6115 BlpI GC'TnA_GC Cuts at: 3532 3532 Size: 6115 Bme1580I G_kGCm'C Cuts at: 1790 2060 4176 5332 1790 Size: 270 2116 1156 2573 Fragments arranged by size: 2573 2116 1156 270 BmgBI CAC'GTC Cuts at: 1318 3223 1318 Size: 1905 4210 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3846 4804 442 Size: 416 53 83 399 24 2429 958 1753 Fragments arranged by size: 2429 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2493 1056 Size: 1437 4678 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2556 4775 1239 Size: 1317 2219 2579 Fragments arranged by size: 2579 2219 1317 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2015 2240 4240 5105 5403 2015 Size: 225 2000 865 298 2727 Fragments arranged by size: 2727 2000 865 298 225 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2932 4778 882 Size: 617 1433 1846 2219 Fragments arranged by size: 2219 1846 1433 617 BsaAI yAC'GTr Cuts at: 493 1204 3277 3906 493 Size: 711 2073 629 2702 Fragments arranged by size: 2702 2073 711 629 BsaBI GATnn'nnATC Cuts at: 6109 6109 Size: 6115 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3922 4304 4939 275 Size: 53 83 186 936 2389 382 635 1451 Fragments arranged by size: 2389 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4548 4946 5220 5367 5778 4548 Size: 398 274 147 411 4885 Fragments arranged by size: 4885 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2255 2285 1178 Size: 30 165 30 852 30 5008 Fragments arranged by size: 5008 852 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6115 BseYI C'CCAG_C Cuts at: 2378 5318 5814 2378 Size: 2940 496 2679 Fragments arranged by size: 2940 2679 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2109 1163 Size: 573 373 5169 Fragments arranged by size: 5169 573 373 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3565 4326 5354 1540 Size: 275 186 1564 761 1028 2301 Fragments arranged by size: 2301 1564 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2242 4176 4261 5332 2242 Size: 1934 85 1071 3025 Fragments arranged by size: 3025 1934 1071 85 BsmI GAATG_Cn' Cuts at: 2593 3626 3719 2593 Size: 1033 93 4989 Fragments arranged by size: 4989 1033 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2242 3434 4176 4261 5332 1391 Size: 399 270 182 1192 742 85 1071 2174 Fragments arranged by size: 2174 1192 1071 742 399 270 182 85 BspEI T'CCGG_A Cuts at: 5778 5778 Size: 6115 BspHI T'CATG_A Cuts at: 4004 5734 4004 Size: 1730 4385 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3470 844 Size: 2626 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4002 1394 Size: 429 2179 3507 Fragments arranged by size: 3507 2179 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4610 4784 65 Size: 1183 3362 174 1396 Fragments arranged by size: 3362 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4759 4946 1700 Size: 110 2949 187 2869 Fragments arranged by size: 2949 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6115 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6115 BssSI C'ACGA_G Cuts at: 2454 4175 5187 2454 Size: 1721 1012 3382 Fragments arranged by size: 3382 1721 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6115 BstBI TT'CG_AA Cuts at: 4919 4919 Size: 6115 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6115 BstYI r'GATC_y Cuts at: 1972 3044 3445 3812 4197 4214 5666 5752 5764 Size: 1072 401 367 385 17 1452 86 12 Cuts at: 5764 6110 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1072 401 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2274 513 Size: 552 939 270 4354 Fragments arranged by size: 4354 939 552 270 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5376 Fragments arranged by size: 5376 683 56 BtsI GCAGTG_nn' Cuts at: 2239 3631 4425 4445 2239 Size: 1392 794 20 3909 Fragments arranged by size: 3909 1392 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6080 DraI TTT'AAA Cuts at: 2929 3331 3419 2929 Size: 402 88 5625 Fragments arranged by size: 5625 402 88 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5122 809 Size: 507 3806 1802 Fragments arranged by size: 3806 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 2332 2421 Size: 54 1425 50 275 18 351 151 89 Cuts at: 2421 3562 4451 6080 8 Size: 1141 889 1629 43 Fragments arranged by size: 1629 1425 1141 889 351 275 151 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3562 1537 Size: 275 1750 4090 Fragments arranged by size: 4090 1750 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2821 3010 4045 1353 Size: 1468 189 1035 3423 Fragments arranged by size: 3423 1468 1035 189 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3160 4702 5076 5222 387 Size: 1064 138 1571 1542 374 146 1280 Fragments arranged by size: 1571 1542 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2240 2240 Size: 6115 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2465 2556 2717 2802 4178 4775 5562 Size: 393 833 91 161 85 1376 597 787 Cuts at: 5562 5830 1239 Size: 268 1524 Fragments arranged by size: 1524 1376 833 787 597 393 268 161 91 85 EcoRI G'AATT_C Cuts at: 2442 3424 3802 2442 Size: 982 378 4755 Fragments arranged by size: 4755 982 378 EcoRV GAT'ATC Cuts at: 2614 2614 Size: 6115 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2281 3360 2281 Size: 1079 5036 FspI TGC'GCA Cuts at: 4621 4621 Size: 6115 HaeII r_GCGC'y Cuts at: 1678 5262 1678 Size: 3584 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2179 2211 4756 4788 4830 Size: 32 135 32 607 32 2545 32 42 Cuts at: 4830 4862 1373 Size: 32 2626 Fragments arranged by size: 2626 2545 607 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2773 3459 3705 669 Size: 988 281 835 686 246 3079 Fragments arranged by size: 3079 988 835 686 281 246 HindIII A'AGCT_T Cuts at: 748 2221 748 Size: 1473 4642 HpaI GTT'AAC Cuts at: 2773 3705 2773 Size: 932 5183 KpnI G_GTAC'C Cuts at: 3440 3440 Size: 6115 MfeI C'AATT_G Cuts at: 3714 3714 Size: 6115 MluI A'CGCG_T Cuts at: 5991 5991 Size: 6115 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5229 5413 1305 Size: 3924 184 2007 Fragments arranged by size: 3924 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2183 2334 2423 10 Size: 54 1425 343 351 151 89 3702 Fragments arranged by size: 3702 1425 351 343 151 89 54 MslI CAynn'nnrTG Cuts at: 518 1166 2138 2866 3221 4073 4432 4591 518 Size: 648 972 728 355 852 359 159 2042 Fragments arranged by size: 2042 972 852 728 648 359 355 159 MspA1I CmG'CkG Cuts at: 1606 2382 2971 3020 3297 3531 4208 5356 5601 Size: 776 589 49 277 234 677 1148 245 Cuts at: 5601 1606 Size: 2120 Fragments arranged by size: 2120 1148 776 677 589 277 245 234 49 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4624 Fragments arranged by size: 4624 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6115 NheI G'CTAG_C Cuts at: 1052 2489 1052 Size: 1437 4678 NotI GC'GGCC_GC Cuts at: 3562 3562 Size: 6115 NsiI A_TGCA'T Cuts at: 2729 2729 Size: 6115 NspI r_CATG'y Cuts at: 1132 1229 2673 3473 5018 1132 Size: 97 1444 800 1545 2229 Fragments arranged by size: 2229 1545 1444 800 97 PasI CC'CwG_GG Cuts at: 1348 2802 1348 Size: 1454 4661 PciI A'CATG_T Cuts at: 5014 5014 Size: 6115 PflMI CCAn_nnn'nTGG Cuts at: 1108 2234 2430 1108 Size: 1126 196 4793 Fragments arranged by size: 4793 1126 196 PmeI GTTT'AAAC Cuts at: 3419 3419 Size: 6115 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4184 4216 5697 5729 4184 Size: 32 1481 32 4570 Fragments arranged by size: 4570 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6115 PsiI TTA'TAA Cuts at: 3180 3685 3180 Size: 505 5610 PstI C_TGCA'G Cuts at: 830 2209 3467 830 Size: 1379 1258 3478 Fragments arranged by size: 3478 1379 1258 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6115 PvuII CAG'CTG Cuts at: 2382 2971 3020 2382 Size: 589 49 5477 Fragments arranged by size: 5477 589 49 SacI G_AGCT'C Cuts at: 2242 2242 Size: 6115 SalI G'TCGA_C Cuts at: 1936 3457 1936 Size: 1521 4594 SbfI CC_TGCA'GG Cuts at: 3467 3467 Size: 6115 ScaI AGT'ACT Cuts at: 4363 6075 4363 Size: 1712 4403 SfcI C'TryA_G Cuts at: 826 1045 2205 2754 2826 3463 4598 5279 5470 Size: 219 1160 549 72 637 1135 681 191 Cuts at: 5470 826 Size: 1471 Fragments arranged by size: 1471 1160 1135 681 637 549 219 191 72 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6115 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6115 SmaI CCC'GGG Cuts at: 1872 3442 1872 Size: 1570 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2030 2255 4219 5120 5382 820 Size: 197 926 87 225 1964 901 262 1553 Fragments arranged by size: 1964 1553 926 901 262 225 197 87 SnaBI TAC'GTA Cuts at: 493 3906 493 Size: 3413 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6115 SphI G_CATG'C Cuts at: 1132 2673 3473 1132 Size: 1541 800 3774 Fragments arranged by size: 3774 1541 800 SspI AAT'ATT Cuts at: 5 52 3388 4039 5 Size: 47 3336 651 2081 Fragments arranged by size: 3336 2081 651 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6115 StyI C'CwwG_G Cuts at: 513 1065 2004 2641 3554 513 Size: 552 939 637 913 3074 Fragments arranged by size: 3074 939 913 637 552 SwaI ATTT'AAAT Cuts at: 2929 2929 Size: 6115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2815 4142 4159 4312 4497 4942 325 Size: 1255 1235 1327 17 153 185 445 1498 Fragments arranged by size: 1498 1327 1255 1235 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2355 4361 6073 Size: 274 80 33 51 1443 378 2006 1712 Cuts at: 6073 96 Size: 138 Fragments arranged by size: 2006 1712 1443 378 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 6075 6107 2085 Size: 32 3958 32 2093 Fragments arranged by size: 3958 2093 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6115 XbaI T'CTAG_A Cuts at: 3451 3451 Size: 6115 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2116 2116 Size: 6115 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6115 XmaI C'CCGG_G Cuts at: 1870 3440 1870 Size: 1570 4545 XmnI GAAnn'nnTTC Cuts at: 2580 4244 2580 Size: 1664 4451 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3923 276 Size: 53 83 186 936 2389 2468 Fragments arranged by size: 2468 2389 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI