(Linear) (Six-base) MAPSORT of: KIEE6474.seq Check: 4717 from: 1 to: 1410 KIEE6474.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AccI GT'mk_AC Cuts at: 0 1268 1410 Size: 1268 142 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 712 797 1410 Size: 460 252 85 613 Fragments arranged by size: 613 460 252 85 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 141 1410 Size: 141 1269 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 696 728 1410 Size: 696 32 682 Fragments arranged by size: 696 682 32 AlwNI CAG_nnn'CTG Cuts at: 0 820 1410 Size: 820 590 ApoI r'AATT_y Cuts at: 0 89 437 803 1410 Size: 89 348 366 607 Fragments arranged by size: 607 366 348 89 AseI AT'TA_AT Cuts at: 0 71 1120 1410 Size: 71 1049 290 Fragments arranged by size: 1049 290 71 BamHI G'GATC_C Cuts at: 0 1039 1410 Size: 1039 371 BanII G_rGCy'C Cuts at: 0 237 1410 Size: 237 1173 BfrBI ATG'CAT Cuts at: 0 722 1410 Size: 722 688 BglI GCCn_nnn'nGGC Cuts at: 0 175 959 1410 Size: 175 784 451 Fragments arranged by size: 784 451 175 Bme1580I G_kGCm'C Cuts at: 0 55 1410 Size: 55 1355 BmgBI CAC'GTC Cuts at: 0 1218 1410 Size: 1218 192 BmtI G_CTAG'C Cuts at: 0 488 1410 Size: 488 922 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 551 1410 Size: 551 859 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 10 235 1410 Size: 10 225 1175 Fragments arranged by size: 1175 225 10 BsaI GGTCTCn'nnnn_ Cuts at: 0 927 1410 Size: 927 483 BsaAI yAC'GTr Cuts at: 0 1272 1410 Size: 1272 138 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 250 280 1410 Size: 250 30 1130 Fragments arranged by size: 1130 250 30 BseYI C'CCAG_C Cuts at: 0 373 1410 Size: 373 1037 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 104 1410 Size: 104 1306 BsiHKAI G_wGCw'C Cuts at: 0 237 1410 Size: 237 1173 BsmI GAATG_Cn' Cuts at: 0 588 1410 Size: 588 822 Bsp1286I G_dGCh'C Cuts at: 0 55 237 1410 Size: 55 182 1173 Fragments arranged by size: 1173 182 55 BssSI C'ACGA_G Cuts at: 0 449 1410 Size: 449 961 BstYI r'GATC_y Cuts at: 0 1039 1410 Size: 1039 371 BtgI C'CryG_G Cuts at: 0 269 1410 Size: 269 1141 BtsI GCAGTG_nn' Cuts at: 0 234 1410 Size: 234 1176 DraI TTT'AAA Cuts at: 0 924 1326 1410 Size: 924 402 84 Fragments arranged by size: 924 402 84 EaeI y'GGCC_r Cuts at: 0 176 327 416 1410 Size: 176 151 89 994 Fragments arranged by size: 994 176 151 89 EarI CTCTTCn'nnn_ Cuts at: 0 816 1005 1410 Size: 816 189 405 Fragments arranged by size: 816 405 189 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1155 1410 Size: 1155 255 EcoICRI GAG'CTC Cuts at: 0 235 1410 Size: 235 1175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 551 712 797 1410 Size: 460 91 161 85 613 Fragments arranged by size: 613 460 161 91 85 EcoRI G'AATT_C Cuts at: 0 437 1410 Size: 437 973 EcoRV GAT'ATC Cuts at: 0 609 1410 Size: 609 801 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 276 1355 1410 Size: 276 1079 55 Fragments arranged by size: 1079 276 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 174 206 1410 Size: 174 32 1204 Fragments arranged by size: 1204 174 32 HincII GTy'rAC Cuts at: 0 768 1410 Size: 768 642 HindIII A'AGCT_T Cuts at: 0 216 1410 Size: 216 1194 HpaI GTT'AAC Cuts at: 0 768 1410 Size: 768 642 MscI TGG'CCA Cuts at: 0 178 329 418 1410 Size: 178 151 89 992 Fragments arranged by size: 992 178 151 89 MslI CAynn'nnrTG Cuts at: 0 133 861 1216 1410 Size: 133 728 355 194 Fragments arranged by size: 728 355 194 133 MspA1I CmG'CkG Cuts at: 0 377 966 1015 1292 1410 Size: 377 589 49 277 118 Fragments arranged by size: 589 377 277 118 49 NheI G'CTAG_C Cuts at: 0 484 1410 Size: 484 926 NsiI A_TGCA'T Cuts at: 0 724 1410 Size: 724 686 NspI r_CATG'y Cuts at: 0 668 1410 Size: 668 742 PasI CC'CwG_GG Cuts at: 0 797 1410 Size: 797 613 PflMI CCAn_nnn'nTGG Cuts at: 0 229 425 1410 Size: 229 196 985 Fragments arranged by size: 985 229 196 PsiI TTA'TAA Cuts at: 0 1175 1410 Size: 1175 235 PstI C_TGCA'G Cuts at: 0 204 1410 Size: 204 1206 PvuII CAG'CTG Cuts at: 0 377 966 1015 1410 Size: 377 589 49 395 Fragments arranged by size: 589 395 377 49 SacI G_AGCT'C Cuts at: 0 237 1410 Size: 237 1173 SfcI C'TryA_G Cuts at: 0 200 749 821 1410 Size: 200 549 72 589 Fragments arranged by size: 589 549 200 72 SmlI C'TyrA_G Cuts at: 0 25 250 1410 Size: 25 225 1160 Fragments arranged by size: 1160 225 25 SphI G_CATG'C Cuts at: 0 668 1410 Size: 668 742 SspI AAT'ATT Cuts at: 0 1383 1410 Size: 1383 27 StyI C'CwwG_G Cuts at: 0 636 1410 Size: 636 774 SwaI ATTT'AAAT Cuts at: 0 924 1410 Size: 924 486 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 810 1410 Size: 810 600 TatI w'GTAC_w Cuts at: 0 350 1410 Size: 350 1060 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 80 112 1410 Size: 80 32 1298 Fragments arranged by size: 1298 80 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 111 1410 Size: 111 1299 XmnI GAAnn'nnTTC Cuts at: 0 575 1410 Size: 575 835 Enzymes that do cut: AccI AcuI AlfI AloI AlwNI ApoI AseI BamHI BanII BfrBI BglI Bme1580I BmgBI BmtI BpmI BpuEI BsaI BsaAI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BssSI BstYI BtgI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI Hin4I HincII HindIII HpaI MscI MslI MspA1I NheI NsiI NspI PasI PflMI PsiI PstI PvuII SacI SfcI SmlI SphI SspI StyI SwaI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI BmrI BplI Bpu10I BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EcoNI EcoO109I FseI FspI FspAI HaeII KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NotI NruI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI Tth111I XbaI XhoI XmaI ZraI