################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmFI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI HinP1I HindIII KasI KpnI KspI MaeI MamI MfeI MflI MluI MroI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 538 GACGTC GACGT^C AciI 515 GCGG CCGC(-3/-1) AcsI 416 AAATTC [GA]^AATT[CT] AcyI 538 GACGTC G[GA]^CG[CT]C AfaI 157 GTAC GT^AC AflIII 678 ACATGT A^C[GA][CT]GT AlwNI 296 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 740 GGGCCC GGGCC^C ApoI 416 AAATTC [GA]^AATT[CT] AseI 693 ATTAAT AT^TAAT AsnI 693 ATTAAT AT^TAAT AspI 394 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 728 TGGCCA TGG^CCA BanII 740 GGGCCC G[GA]GC[CT]^C BclI 387 TGATCA T^GATCA BmyI 740 GGGCCC G[AGT]GC[ACT]^C BpmI 17 CTGGAG CTGGAG(16/14) BsaHI 538 GACGTC G[GA]^CG[CT]C BsmAI 541 GTCTC GTCTC(1/5) BsmBI 540 CGTCTC CGTCTC(1/5) BsmI 718 GCATTC GAATGC(1/-1) Bsp1286I 740 GGGCCC G[AGT]GC[ACT]^C BspHI 390 TCATGA T^CATGA BspLU11I 678 ACATGT A^CATGT BstAPI 346 GCATACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EaeI 728 TGGCCA [CT]^GGCC[GA] EcoT14I 505 CCTTGG C^C[AT][AT]GG FbaI 387 TGATCA T^GATCA Hin1I 538 GACGTC G[GA]^CG[CT]C HpaI 231 GTTAAC GTT^AAC MaeII 539 ACGT A^CGT MaeIII 553 GTCAC ^GT[ACGT]AC MluNI 728 TGGCCA TGG^CCA MscI 728 TGGCCA TGG^CCA MslI 391 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflFI 394 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 693 ATTAAT AT^TAAT PstI 701 CTGCAG CTGCA^G RcaI 390 TCATGA T^CATGA RsaI 157 GTAC GT^AC SfcI 701 CTGCAG C^T[GA][CT]AG StyI 505 CCTTGG C^C[AT][AT]GG TaqI 564 TCGA T^CGA Tsp45I 553 GTCAC ^GT[GC]AC TspRI 704 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 394 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 564 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 249 GATCC GGATC(4/5) 444 GGATC BcnI 252 CCCGG CC^[GC]GG 723 CCGGG BsrDI 351 CATTGC GCAATG(2/0) 557 CATTGC BsrI 16 ACTGG ACTGG(1/-1) 596 ACTGG Cac8I 497 GCCAGC GC[ACGT]^[ACGT]GC 780 GCTTGC DraII 739 AGGGCCC [GA]G^G[ACGT]CC[CT] 740 GGGCCCC DsaI 39 CCACGG C^C[GA][CT]GG 151 CCACGG EarI 490 GAAGAG CTCTTC(1/4) 648 GAAGAG EcoO109I 739 AGGGCCC [GA]G^G[ACGT]CC[CT] 740 GGGCCCC HapII 253 CCGG C^CGG 723 CCGG HincII 231 GTTAAC GT[CT]^[GA]AC 682 GTCAAC HindII 231 GTTAAC GT[CT]^[GA]AC 682 GTCAAC HpaII 253 CCGG C^CGG 723 CCGG HphI 517 GGTGA GGTGA(8/7) 527 TCACC Ksp632I 490 GAAGAG CTCTTC(1/4) 648 GAAGAG MspI 253 CCGG C^CGG 723 CCGG MwoI 346 GCATACATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 501 GCAACCTTGGC NciI 252 CCCGG CC^[GC]GG 723 CCGGG NlaIV 740 GGGCCC GG[ACGT]^[ACGT]CC 741 GGCCCC NspI 140 GCATGT [GA]CATG^[CT] 678 ACATGT SfaNI 196 GATGC GCATC(5/9) 561 GCATC TfiI 273 GATTC G^A[AT]TC 520 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AluI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MboI MboII MnlI MseI MvaI NdeII NlaIII PleI Sau3AI Sau96I ScrFI Tru9I TseI Tsp509I