(Circular) (Six-base) MAPSORT of: pFN21AE6665.seq Check: 8859 from: 1 to: 5575 pFN21AE6665 5575 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2547 3385 278 Size: 53 83 186 936 1011 838 2468 Fragments arranged by size: 2468 1011 936 838 186 83 53 AccI GT'mk_AC Cuts at: 1937 2918 1937 Size: 981 4594 Acc65I G'GTAC_C Cuts at: 2896 2896 Size: 5575 AclI AA'CG_TT Cuts at: 1563 3702 4075 1563 Size: 2139 373 3063 Fragments arranged by size: 3063 2139 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 2346 2594 3638 5022 5290 1632 Size: 701 13 248 1044 1384 268 1917 Fragments arranged by size: 1917 1384 1044 701 268 248 13 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5378 AflIII A'CryG_T Cuts at: 1203 2683 4474 5451 1203 Size: 1480 1791 977 1327 Fragments arranged by size: 1791 1480 1327 977 AgeI A'CCGG_T Cuts at: 4406 4406 Size: 5575 AhdI GACnn_n'nnGTC Cuts at: 4304 4304 Size: 5575 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5575 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5575 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 2085 Size: 32 5543 AlwNI CAG_nnn'CTG Cuts at: 2306 3318 4890 2306 Size: 1012 1572 2991 Fragments arranged by size: 2991 1572 1012 ApaI G_GGCC'C Cuts at: 2749 2749 Size: 5575 ApaLI G'TGCA_C Cuts at: 3632 4788 3632 Size: 1156 4419 ApoI r'AATT_y Cuts at: 1493 2421 2884 3110 3262 5371 1493 Size: 928 463 226 152 2109 1697 Fragments arranged by size: 2109 1697 928 463 226 152 AseI AT'TA_AT Cuts at: 160 1032 2699 4129 160 Size: 872 1667 1430 1606 Fragments arranged by size: 1667 1606 1430 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 AvaI C'yCGr_G Cuts at: 1870 1943 2900 1870 Size: 73 957 4545 Fragments arranged by size: 4545 957 73 BamHI G'GATC_C Cuts at: 2905 3272 2905 Size: 367 5208 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2896 4351 618 Size: 325 217 627 1109 1455 1842 Fragments arranged by size: 1842 1455 1109 627 325 217 BanII G_rGCy'C Cuts at: 1391 2749 2894 1391 Size: 1358 145 4072 Fragments arranged by size: 4072 1358 145 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4604 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3766 3800 1903 Size: 34 50 34 1745 34 3678 Fragments arranged by size: 3678 1745 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3467 4677 3467 Size: 1210 4365 BclI T'GATC_A Cuts at: 1548 2392 1548 Size: 844 4731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4186 136 Size: 107 122 71 631 605 2514 1525 Fragments arranged by size: 2514 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5570 5570 Size: 5575 BlpI GC'TnA_GC Cuts at: 2992 2992 Size: 5575 Bme1580I G_kGCm'C Cuts at: 1790 2749 3636 4792 1790 Size: 959 887 1156 2573 Fragments arranged by size: 2573 1156 959 887 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5575 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2611 3306 4264 Size: 416 53 83 399 24 1194 695 958 Cuts at: 4264 442 Size: 1753 Fragments arranged by size: 1753 1194 958 695 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5575 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2043 4235 1239 Size: 804 2192 2579 Fragments arranged by size: 2579 2192 804 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3700 4565 4863 3700 Size: 865 298 4412 Fragments arranged by size: 4412 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4238 882 Size: 617 2739 2219 Fragments arranged by size: 2739 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3366 493 Size: 711 2162 2702 Fragments arranged by size: 2702 2162 711 BsaBI GATnn'nnATC Cuts at: 5569 5569 Size: 5575 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2544 3382 3764 4399 Size: 53 83 186 936 1011 838 382 635 Cuts at: 4399 275 Size: 1451 Fragments arranged by size: 1451 1011 936 838 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4008 4406 4680 4827 5238 4008 Size: 398 274 147 411 4345 Fragments arranged by size: 4345 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5350 Fragments arranged by size: 5350 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5575 BseYI C'CCAG_C Cuts at: 4778 5274 4778 Size: 496 5079 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5002 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3025 3786 4814 1540 Size: 275 186 1024 761 1028 2301 Fragments arranged by size: 2301 1028 1024 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3636 3721 4792 3636 Size: 85 1071 4419 Fragments arranged by size: 4419 1071 85 BsmI GAATG_Cn' Cuts at: 2723 3086 3179 2723 Size: 363 93 5119 Fragments arranged by size: 5119 363 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2551 2551 Size: 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2749 2894 3636 3721 4792 1391 Size: 399 959 145 742 85 1071 2174 Fragments arranged by size: 2174 1071 959 742 399 145 85 BspEI T'CCGG_A Cuts at: 5238 5238 Size: 5575 BspHI T'CATG_A Cuts at: 2395 3464 5194 2395 Size: 1069 1730 2776 Fragments arranged by size: 2776 1730 1069 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2930 844 Size: 2086 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3462 1394 Size: 429 1639 3507 Fragments arranged by size: 3507 1639 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2355 2561 4070 4244 65 Size: 1183 1107 206 1509 174 1396 Fragments arranged by size: 1509 1396 1183 1107 206 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4219 4406 1700 Size: 110 2409 187 2869 Fragments arranged by size: 2869 2409 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5575 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5575 BssSI C'ACGA_G Cuts at: 3635 4647 3635 Size: 1012 4563 BstAPI GCAn_nnn'nTGC Cuts at: 794 2357 794 Size: 1563 4012 BstBI TT'CG_AA Cuts at: 4379 4379 Size: 5575 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5575 BstYI r'GATC_y Cuts at: 1972 2905 3272 3657 3674 5126 5212 5224 5570 Size: 933 367 385 17 1452 86 12 346 Cuts at: 5570 1972 Size: 1977 Fragments arranged by size: 1977 1452 933 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2044 2156 513 Size: 552 979 112 3932 Fragments arranged by size: 3932 979 552 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4836 Fragments arranged by size: 4836 683 56 BtsI GCAGTG_nn' Cuts at: 2715 3091 3885 3905 2715 Size: 376 794 20 4385 Fragments arranged by size: 4385 794 376 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5575 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5540 DraI TTT'AAA Cuts at: 2879 2879 Size: 5575 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4582 809 Size: 507 3266 1802 Fragments arranged by size: 3266 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2733 3022 3911 Size: 54 1425 50 275 18 903 289 889 Cuts at: 3911 5540 8 Size: 1629 43 Fragments arranged by size: 1629 1425 903 889 289 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3022 1537 Size: 275 1210 4090 Fragments arranged by size: 4090 1210 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2490 2648 3505 1353 Size: 1137 158 857 3423 Fragments arranged by size: 3423 1137 857 158 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4162 4536 4682 387 Size: 1064 138 2573 374 146 1280 Fragments arranged by size: 2573 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2333 2346 2594 3638 4235 5022 Size: 393 411 290 13 248 1044 597 787 Cuts at: 5022 5290 1239 Size: 268 1524 Fragments arranged by size: 1524 1044 787 597 411 393 290 268 248 13 EcoO109I rG'GnC_Cy Cuts at: 2745 2746 2745 Size: 1 5574 EcoRI G'AATT_C Cuts at: 2884 3262 2884 Size: 378 5197 FspI TGC'GCA Cuts at: 4081 4081 Size: 5575 HaeII r_GCGC'y Cuts at: 1678 4722 1678 Size: 3044 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4216 4248 4290 4322 1373 Size: 32 135 32 2644 32 42 32 2626 Fragments arranged by size: 2644 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2238 2689 2919 3165 669 Size: 988 281 300 451 230 246 3079 Fragments arranged by size: 3079 988 451 300 281 246 230 HindIII A'AGCT_T Cuts at: 748 748 Size: 5575 HpaI GTT'AAC Cuts at: 2238 3165 2238 Size: 927 4648 KpnI G_GTAC'C Cuts at: 2900 2900 Size: 5575 MfeI C'AATT_G Cuts at: 3174 3174 Size: 5575 MluI A'CGCG_T Cuts at: 5451 5451 Size: 5575 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2415 4689 4873 1305 Size: 1110 2274 184 2007 Fragments arranged by size: 2274 2007 1110 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2735 10 Size: 54 1425 343 903 2850 Fragments arranged by size: 2850 1425 903 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2400 3533 3892 4051 518 Size: 648 1234 1133 359 159 2042 Fragments arranged by size: 2042 1234 1133 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2991 3668 4816 5061 1606 Size: 1385 677 1148 245 2120 Fragments arranged by size: 2120 1385 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5023 NdeI CA'TA_TG Cuts at: 387 387 Size: 5575 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5575 NotI GC'GGCC_GC Cuts at: 3022 3022 Size: 5575 NspI r_CATG'y Cuts at: 1132 1229 2149 2687 2933 4478 1132 Size: 97 920 538 246 1545 2229 Fragments arranged by size: 2229 1545 920 538 246 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5575 PciI A'CATG_T Cuts at: 2683 4474 2683 Size: 1791 3784 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5575 PfoI T'CCnGG_A Cuts at: 2103 2103 Size: 5575 PmeI GTTT'AAAC Cuts at: 2879 2879 Size: 5575 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3644 3676 5157 5189 3644 Size: 32 1481 32 4030 Fragments arranged by size: 4030 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5575 PsiI TTA'TAA Cuts at: 3145 3145 Size: 5575 PspOMI G'GGCC_C Cuts at: 2745 2745 Size: 5575 PstI C_TGCA'G Cuts at: 830 2710 2927 830 Size: 1880 217 3478 Fragments arranged by size: 3478 1880 217 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5575 SalI G'TCGA_C Cuts at: 1936 2917 1936 Size: 981 4594 SbfI CC_TGCA'GG Cuts at: 2927 2927 Size: 5575 ScaI AGT'ACT Cuts at: 3823 5535 3823 Size: 1712 3863 SfcI C'TryA_G Cuts at: 826 1045 2706 2923 4058 4739 4930 826 Size: 219 1661 217 1135 681 191 1471 Fragments arranged by size: 1661 1471 1135 681 219 217 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5575 SmaI CCC'GGG Cuts at: 1872 2902 1872 Size: 1030 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3679 4580 4842 820 Size: 197 926 1736 901 262 1553 Fragments arranged by size: 1736 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3366 493 Size: 2873 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5575 SphI G_CATG'C Cuts at: 1132 2933 1132 Size: 1801 3774 SspI AAT'ATT Cuts at: 5 52 3499 5 Size: 47 3447 2081 Fragments arranged by size: 3447 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5575 StyI C'CwwG_G Cuts at: 513 1065 2510 3014 513 Size: 552 1445 504 3074 Fragments arranged by size: 3074 1445 552 504 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2134 3602 3619 3772 3957 4402 325 Size: 1255 554 1468 17 153 185 445 1498 Fragments arranged by size: 1498 1468 1255 554 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3821 5533 96 Size: 274 80 33 51 1443 1844 1712 138 Fragments arranged by size: 1844 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5535 5567 5535 Size: 32 5543 Tth111I GACn'n_nGTC Cuts at: 1316 2402 1316 Size: 1086 4489 XbaI T'CTAG_A Cuts at: 2911 2911 Size: 5575 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5575 XmaI C'CCGG_G Cuts at: 1870 2900 1870 Size: 1030 4545 XmnI GAAnn'nnTTC Cuts at: 3704 3704 Size: 5575 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2545 3383 276 Size: 53 83 186 936 1011 838 2468 Fragments arranged by size: 2468 1011 936 838 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI