(Linear) (Six-base) MAPSORT of: KIEE6665.seq  Check: 8068  from: 1  to: 870



KIEE6665.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:35  ..

AatII G_ACGT'C

Cuts at:      0     542     870 
   Size:        542     328
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     341     589     870 
   Size:        328      13     248     281

  Fragments arranged by size: 

                 328     281     248      13
 
AflIII A'CryG_T

Cuts at:      0     678     870 
   Size:        678     192
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      80     112     870 
   Size:         80      32     758

  Fragments arranged by size: 

                 758      80      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     301     870 
   Size:        301     569
 
ApaI G_GGCC'C

Cuts at:      0     744     870 
   Size:        744     126
 
ApoI r'AATT_y

Cuts at:      0     416     870 
   Size:        416     454
 
AseI AT'TA_AT

Cuts at:      0     694     870 
   Size:        694     176
 
BanII G_rGCy'C

Cuts at:      0     744     870 
   Size:        744     126
 
BclI T'GATC_A

Cuts at:      0     387     870 
   Size:        387     483
 
Bme1580I G_kGCm'C

Cuts at:      0     744     870 
   Size:        744     126
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     606     870 
   Size:        606     264
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     870 
   Size:         38     832
 
BsaHI Gr'CG_yC

Cuts at:      0     539     870 
   Size:        539     331
 
BsmI GAATG_Cn'

Cuts at:      0     718     870 
   Size:        718     152
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     546     870 
   Size:        546     324
 
Bsp1286I G_dGCh'C

Cuts at:      0     744     870 
   Size:        744     126
 
BspHI T'CATG_A

Cuts at:      0     390     870 
   Size:        390     480
 
BsrDI GCAATG_nn'

Cuts at:      0     350     556     870 
   Size:        350     206     314

  Fragments arranged by size: 

                 350     314     206
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     352     870 
   Size:        352     518
 
BtgI C'CryG_G

Cuts at:      0      39     151     870 
   Size:         39     112     719

  Fragments arranged by size: 

                 719     112      39
 
BtsI GCAGTG_nn'

Cuts at:      0     710     870 
   Size:        710     160
 
EaeI y'GGCC_r

Cuts at:      0     728     870 
   Size:        728     142
 
EarI CTCTTCn'nnn_

Cuts at:      0     485     643     870 
   Size:        485     158     227

  Fragments arranged by size: 

                 485     227     158
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     328     341     589     870 
   Size:         38     290      13     248     281

  Fragments arranged by size: 

                 290     281     248      38      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0     740     741     870 
   Size:        740       1     129

  Fragments arranged by size: 

                 740     129       1
 
HincII GTy'rAC

Cuts at:      0     233     684     870 
   Size:        233     451     186

  Fragments arranged by size: 

                 451     233     186
 
HpaI GTT'AAC

Cuts at:      0     233     870 
   Size:        233     637
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     410     870 
   Size:        410     460
 
MscI TGG'CCA

Cuts at:      0     730     870 
   Size:        730     140
 
MslI CAynn'nnrTG

Cuts at:      0     395     870 
   Size:        395     475
 
NspI r_CATG'y

Cuts at:      0     144     682     870 
   Size:        144     538     188

  Fragments arranged by size: 

                 538     188     144
 
PciI A'CATG_T

Cuts at:      0     678     870 
   Size:        678     192
 
PfoI T'CCnGG_A

Cuts at:      0      98     870 
   Size:         98     772
 
PspOMI G'GGCC_C

Cuts at:      0     740     870 
   Size:        740     130
 
PstI C_TGCA'G

Cuts at:      0     705     870 
   Size:        705     165
 
SfcI C'TryA_G

Cuts at:      0     701     870 
   Size:        701     169
 
StyI C'CwwG_G

Cuts at:      0     505     870 
   Size:        505     365
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     129     870 
   Size:        129     741
 
Tth111I GACn'n_nGTC

Cuts at:      0     397     870 
   Size:        397     473
 
ZraI GAC'GTC

Cuts at:      0     540     870 
   Size:        540     330
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AloI    AlwNI     ApaI     ApoI     AseI
    BanII     BclI Bme1580I     BmrI     BpmI    BsaHI     BsmI    BsmBI
 Bsp1286I    BspHI    BsrDI   BstAPI     BtgI     BtsI     EaeI     EarI
  Eco57MI EcoO109I   HincII     HpaI     MmeI     MscI     MslI     NspI
     PciI     PfoI   PspOMI     PstI     SfcI     StyI    TaqII  Tth111I
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BspEI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     KasI     KpnI     MfeI     MluI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI     XbaI     XcmI
     XhoI     XmaI     XmnI