Restriction map for
pFN21AB9912
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AvaI
AviII AvrII BanII BbeI BbsI
BbvCI BclI BfaI BfrI BglI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsmBI BsoBI Bsp1286I
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I CpoI DraIII DrdI DsaI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KpnI
Ksp632I KspI MaeI MluI MroI
MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 1701 CGCG CG^CG
AcsI 609 AAATTC [GA]^AATT[CT]
AhdI 640 GACTATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 609 AAATTC [GA]^AATT[CT]
Asp700I 593 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 640 GACTATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1147 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 663 TGGCCA TGG^CCA
BbrPI 908 CACGTG CAC^GTG
BcgI 1529 CGAAAGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 702 GTATCC GTATCC(6/5)
BcnI 1472 CCCGG CC^[GC]GG
BglII 1328 AGATCT A^GATCT
BsgI 1747 CTGCAC GTGCAG(16/14)
BsiWI 398 CGTACG C^GTACG
BsmFI 980 GGGAC GGGAC(10/14)
BsmI 350 GAATGC GAATGC(1/-1)
BspDI 878 ATCGAT AT^CGAT
BstUI 1701 CGCG CG^CG
ClaI 878 ATCGAT AT^CGAT
DraI 180 TTTAAA TTT^AAA
EaeI 663 TGGCCA [CT]^GGCC[GA]
Eam1105I 640 GACTATCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1709 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 24 CCCGC CCCGC(4/6)
HapII 1473 CCGG C^CGG
HgaI 885 GCGTC GACGC(5/10)
HincII 1434 GTTGAC GT[CT]^[GA]AC
HindII 1434 GTTGAC GT[CT]^[GA]AC
HindIII 504 AAGCTT A^AGCTT
HpaII 1473 CCGG C^CGG
MfeI 203 CAATTG C^AATTG
MluNI 663 TGGCCA TGG^CCA
MscI 663 TGGCCA TGG^CCA
MspI 1473 CCGG C^CGG
MunI 203 CAATTG C^AATTG
MvnI 1701 CGCG CG^CG
NciI 1472 CCCGG CC^[GC]GG
PflFI 1147 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1752 CCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 908 CACGTG CAC^GTG
PmlI 908 CACGTG CAC^GTG
SmlI 961 CTTGAG C^T[CT][GA]AG
Tth111I 1147 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1752 CCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 593 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwNI 869 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
950 CAGATTCTG
BamHI 813 GGATCC G^GATCC
1050 GGATCC
BanI 115 GGCACC G^G[CT][GA]CC
531 GGCACC
BsaAI 375 CACGTA [CT]AC^GT[GA]
908 CACGTG
BsaBI 871 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
952 GATTCTGATC
BsmAI 991 GAGAC GTCTC(1/5)
462 GTCTC
BstAPI 1237 GCAACCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1698 GCACGCGATGC
DraII 1024 GGGTCCT [GA]G^G[ACGT]CC[CT]
1190 AGGACCT
EcoO109I 1024 GGGTCCT [GA]G^G[ACGT]CC[CT]
1190 AGGACCT
EcoT14I 1071 CCTTGG C^C[AT][AT]GG
1757 CCTTGG
MaeII 376 ACGT A^CGT
909 ACGT
MamI 871 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
952 GATTCTGATC
MslI 345 CACAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1156 CATATAGATG
NdeI 323 CATATG CA^TATG
413 CATATG
PleI 105 GACTC GAGTC(4/5)
1426 GACTC
PpuMI 1024 GGGTCCT [GA]G^G[AT]CC[CT]
1190 AGGACCT
PstI 1232 CTGCAG CTGCA^G
1393 CTGCAG
SspI 1489 AATATT AAT^ATT
1573 AATATT
StyI 1071 CCTTGG C^C[AT][AT]GG
1757 CCTTGG
TaqI 879 TCGA T^CGA
888 TCGA
TthHB8I 879 TCGA T^CGA
888 TCGA
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BpmI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII