(Circular) (Six-base) MAPSORT of: pFN21AB9912.seq Check: 7554 from: 1 to: 6481 pFN21AB9912 6481 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4291 278 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3824 1937 Size: 1887 4594 Acc65I G'GTAC_C Cuts at: 3802 3802 Size: 6481 AclI AA'CG_TT Cuts at: 1563 4608 4981 1563 Size: 3045 373 3063 Fragments arranged by size: 3063 3045 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2469 2497 3051 3162 4544 5928 6196 1632 Size: 837 28 554 111 1382 1384 268 1917 Fragments arranged by size: 1917 1384 1382 837 554 268 111 28 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6284 AflIII A'CryG_T Cuts at: 1203 5380 6357 1203 Size: 4177 977 1327 Fragments arranged by size: 4177 1327 977 AgeI A'CCGG_T Cuts at: 5312 5312 Size: 6481 AhdI GACnn_n'nnGTC Cuts at: 2650 5210 2650 Size: 2560 3921 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6481 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6481 AlwNI CAG_nnn'CTG Cuts at: 2879 2960 4224 5796 2879 Size: 81 1264 1572 3564 Fragments arranged by size: 3564 1572 1264 81 ApaLI G'TGCA_C Cuts at: 4538 5694 4538 Size: 1156 5325 ApoI r'AATT_y Cuts at: 1493 2614 3790 4016 4168 6277 1493 Size: 1121 1176 226 152 2109 1697 Fragments arranged by size: 2109 1697 1176 1121 226 152 AseI AT'TA_AT Cuts at: 160 1032 5035 160 Size: 872 4003 1606 Fragments arranged by size: 4003 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 AvaI C'yCGr_G Cuts at: 1870 1943 3806 1870 Size: 73 1863 4545 Fragments arranged by size: 4545 1863 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3758 3791 3758 Size: 33 6448 BamHI G'GATC_C Cuts at: 2818 3055 3811 4178 2818 Size: 237 756 367 5121 Fragments arranged by size: 5121 756 367 237 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 2536 3802 5257 618 Size: 325 217 627 333 416 1266 1455 1842 Fragments arranged by size: 1842 1455 1266 627 416 333 325 217 BanII G_rGCy'C Cuts at: 1391 3800 1391 Size: 2409 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5510 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3523 3557 4672 4706 1903 Size: 34 1586 34 1115 34 3678 Fragments arranged by size: 3678 1586 1115 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2718 4373 5583 2718 Size: 1655 1210 3616 Fragments arranged by size: 3616 1655 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6481 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5092 136 Size: 107 122 71 631 605 3420 1525 Fragments arranged by size: 3420 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3333 6476 3333 Size: 3143 3338 BlpI GC'TnA_GC Cuts at: 3898 3898 Size: 6481 Bme1580I G_kGCm'C Cuts at: 1790 4542 5698 1790 Size: 2752 1156 2573 Fragments arranged by size: 2752 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6481 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2058 2655 3036 Size: 416 53 83 399 24 641 597 381 Cuts at: 3036 4212 5170 442 Size: 1176 958 1753 Fragments arranged by size: 1753 1176 958 641 597 416 399 381 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3712 3739 3791 5141 1239 Size: 2473 27 52 1350 2579 Fragments arranged by size: 2579 2473 1350 52 27 Bpu10I CC'TnA_GC Cuts at: 3200 3200 Size: 6481 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2987 4606 5471 5769 2987 Size: 1619 865 298 3699 Fragments arranged by size: 3699 1619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5144 882 Size: 617 3645 2219 Fragments arranged by size: 3645 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2382 2915 4272 493 Size: 711 1178 533 1357 2702 Fragments arranged by size: 2702 1357 1178 711 533 BsaBI GATnn'nnATC Cuts at: 2880 2961 6475 2880 Size: 81 3514 2886 Fragments arranged by size: 3514 2886 81 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4288 4670 5305 275 Size: 53 83 186 936 2755 382 635 1451 Fragments arranged by size: 2755 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4914 5312 5586 5733 6144 4914 Size: 398 274 147 411 5251 Fragments arranged by size: 5251 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2206 2236 2280 2310 3660 Size: 30 165 30 803 30 44 30 1350 Cuts at: 3660 3690 1178 Size: 30 3969 Fragments arranged by size: 3969 1350 803 165 44 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6481 BseYI C'CCAG_C Cuts at: 3523 5684 6180 3523 Size: 2161 496 3824 Fragments arranged by size: 3824 2161 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3737 1163 Size: 573 2001 3907 Fragments arranged by size: 3907 2001 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3931 4692 5720 1540 Size: 275 186 1930 761 1028 2301 Fragments arranged by size: 2301 1930 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4542 4627 5698 4542 Size: 85 1071 5325 Fragments arranged by size: 5325 1071 85 BsiWI C'GTAC_G Cuts at: 2403 2403 Size: 6481 BsmI GAATG_Cn' Cuts at: 2361 3992 4085 2361 Size: 1631 93 4757 Fragments arranged by size: 4757 1631 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3800 4542 4627 5698 1391 Size: 399 2010 742 85 1071 2174 Fragments arranged by size: 2174 2010 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6144 6144 Size: 6481 BspHI T'CATG_A Cuts at: 4370 6100 4370 Size: 1730 4751 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3836 844 Size: 2992 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4368 1394 Size: 429 2545 3507 Fragments arranged by size: 3507 2545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4976 5150 65 Size: 1183 3728 174 1396 Fragments arranged by size: 3728 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5125 5312 1700 Size: 110 3315 187 2869 Fragments arranged by size: 3315 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6481 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6481 BssSI C'ACGA_G Cuts at: 4541 5553 4541 Size: 1012 5469 BstAPI GCAn_nnn'nTGC Cuts at: 794 3248 3709 794 Size: 2454 461 3566 Fragments arranged by size: 3566 2454 461 BstBI TT'CG_AA Cuts at: 5285 5285 Size: 6481 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6481 BstYI r'GATC_y Cuts at: 1972 2017 2818 3055 3333 3723 3811 4178 4563 Size: 45 801 237 278 390 88 367 385 Cuts at: 4563 4580 6032 6118 6130 6476 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 801 390 385 367 346 278 237 88 86 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4990 Fragments arranged by size: 4990 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3722 487 Size: 683 56 2496 3246 Fragments arranged by size: 3246 2496 683 56 BtsI GCAGTG_nn' Cuts at: 2158 3997 4791 4811 2158 Size: 1839 794 20 3828 Fragments arranged by size: 3828 1839 794 20 ClaI AT'CG_AT Cuts at: 1554 2884 1554 Size: 1330 5151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6446 DraI TTT'AAA Cuts at: 2187 3785 2187 Size: 1598 4883 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5488 809 Size: 507 4172 1802 Fragments arranged by size: 4172 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2668 3928 4817 Size: 54 1425 50 275 18 838 1260 889 Cuts at: 4817 6446 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1260 889 838 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3928 1537 Size: 275 2116 4090 Fragments arranged by size: 4090 2116 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4411 1353 Size: 3058 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5068 5442 5588 387 Size: 1064 138 3479 374 146 1280 Fragments arranged by size: 3479 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2469 2497 3051 3162 3712 3739 3791 Size: 393 837 28 554 111 550 27 52 Cuts at: 3791 4544 5141 5928 6196 1239 Size: 753 597 787 268 1524 Fragments arranged by size: 1524 837 787 753 597 554 550 393 268 111 52 28 27 EcoNI CCTnn'n_nnAGG Cuts at: 3718 3718 Size: 6481 EcoO109I rG'GnC_Cy Cuts at: 3030 3196 3030 Size: 166 6315 EcoRI G'AATT_C Cuts at: 3790 4168 3790 Size: 378 6103 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3325 3325 Size: 6481 FspI TGC'GCA Cuts at: 4987 4987 Size: 6481 HaeII r_GCGC'y Cuts at: 1678 5628 1678 Size: 3950 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2636 2668 2894 2926 2964 Size: 32 135 32 1064 32 226 32 38 Cuts at: 2964 2996 5122 5154 5196 5228 1373 Size: 32 2126 32 42 32 2626 Fragments arranged by size: 2626 2126 1064 226 135 42 38 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3441 3825 4071 669 Size: 988 281 1503 384 246 3079 Fragments arranged by size: 3079 1503 988 384 281 246 HindIII A'AGCT_T Cuts at: 748 2509 748 Size: 1761 4720 HpaI GTT'AAC Cuts at: 4071 4071 Size: 6481 KpnI G_GTAC'C Cuts at: 3806 3806 Size: 6481 MfeI C'AATT_G Cuts at: 2208 4080 2208 Size: 1872 4609 MluI A'CGCG_T Cuts at: 6357 6357 Size: 6481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 3340 5595 5779 1305 Size: 993 1042 2255 184 2007 Fragments arranged by size: 2255 2007 1042 993 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2670 10 Size: 54 1425 343 838 3821 Fragments arranged by size: 3821 1425 838 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2354 3165 4439 4798 4957 518 Size: 648 843 345 811 1274 359 159 2042 Fragments arranged by size: 2042 1274 843 811 648 359 345 159 MspA1I CmG'CkG Cuts at: 1606 3897 4574 5722 5967 1606 Size: 2291 677 1148 245 2120 Fragments arranged by size: 2291 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4990 Fragments arranged by size: 4990 939 552 NdeI CA'TA_TG Cuts at: 387 2329 2419 387 Size: 1942 90 4449 Fragments arranged by size: 4449 1942 90 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6481 NotI GC'GGCC_GC Cuts at: 3928 3928 Size: 6481 NspI r_CATG'y Cuts at: 1132 1229 3839 5384 1132 Size: 97 2610 1545 2229 Fragments arranged by size: 2610 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6481 PciI A'CATG_T Cuts at: 5380 5380 Size: 6481 PflMI CCAn_nnn'nTGG Cuts at: 1108 3763 1108 Size: 2655 3826 PfoI T'CCnGG_A Cuts at: 3476 3476 Size: 6481 PmeI GTTT'AAAC Cuts at: 3785 3785 Size: 6481 PmlI CAC'GTG Cuts at: 2915 2915 Size: 6481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2960 2992 4550 4582 6063 6095 2960 Size: 32 1558 32 1481 32 3346 Fragments arranged by size: 3346 1558 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3030 3196 3030 Size: 166 6315 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6481 PsiI TTA'TAA Cuts at: 2694 4051 2694 Size: 1357 5124 PstI C_TGCA'G Cuts at: 830 3241 3402 3833 830 Size: 2411 161 431 3478 Fragments arranged by size: 3478 2411 431 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6481 SalI G'TCGA_C Cuts at: 1936 3823 1936 Size: 1887 4594 SbfI CC_TGCA'GG Cuts at: 3833 3833 Size: 6481 ScaI AGT'ACT Cuts at: 4729 6441 4729 Size: 1712 4769 SexAI A'CCwGG_T Cuts at: 3777 3777 Size: 6481 SfcI C'TryA_G Cuts at: 826 1045 2367 3237 3316 3398 3829 4964 5645 Size: 219 1322 870 79 82 431 1135 681 Cuts at: 5645 5836 826 Size: 191 1471 Fragments arranged by size: 1471 1322 1135 870 681 431 219 191 82 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6481 SmaI CCC'GGG Cuts at: 1872 3808 1872 Size: 1936 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2966 4585 5486 5748 820 Size: 197 926 1023 1619 901 262 1553 Fragments arranged by size: 1619 1553 1023 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4272 493 Size: 3779 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6481 SphI G_CATG'C Cuts at: 1132 3839 1132 Size: 2707 3774 SspI AAT'ATT Cuts at: 5 52 3496 3580 4405 5 Size: 47 3444 84 825 2081 Fragments arranged by size: 3444 2081 825 84 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6481 StyI C'CwwG_G Cuts at: 513 1065 2004 3076 3762 3920 513 Size: 552 939 1072 686 158 3074 Fragments arranged by size: 3074 1072 939 686 552 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2875 3076 3443 4508 4525 4678 4863 Size: 1255 1295 201 367 1065 17 153 185 Cuts at: 4863 5308 325 Size: 445 1498 Fragments arranged by size: 1498 1295 1255 1065 445 367 201 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 4727 6439 Size: 274 80 33 51 1443 855 1895 1712 Cuts at: 6439 96 Size: 138 Fragments arranged by size: 1895 1712 1443 855 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2280 2312 2899 2931 3472 3504 3633 3665 6441 Size: 32 587 32 541 32 129 32 2776 Cuts at: 6441 6473 2280 Size: 32 2288 Fragments arranged by size: 2776 2288 587 541 129 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3155 1316 Size: 1839 4642 XbaI T'CTAG_A Cuts at: 3817 3817 Size: 6481 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6481 XmaI C'CCGG_G Cuts at: 1870 3806 1870 Size: 1936 4545 XmnI GAAnn'nnTTC Cuts at: 2602 4610 2602 Size: 2008 4473 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4289 276 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI