(Linear) (Six-base) MAPSORT of: KIBB9912.seq Check: 874 from: 1 to: 1776 KIBB9912.orf With 182 enzymes: SgfI * December 5, 2014 12:55 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 464 492 1046 1157 1776 Size: 464 28 554 111 619 Fragments arranged by size: 619 554 464 111 28 AhdI GACnn_n'nnGTC Cuts at: 0 645 1776 Size: 645 1131 AlwNI CAG_nnn'CTG Cuts at: 0 874 955 1776 Size: 874 81 821 Fragments arranged by size: 874 821 81 ApoI r'AATT_y Cuts at: 0 609 1776 Size: 609 1167 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1753 1776 Size: 1753 23 BamHI G'GATC_C Cuts at: 0 813 1050 1776 Size: 813 237 726 Fragments arranged by size: 813 726 237 BanI G'GyrC_C Cuts at: 0 115 531 1776 Size: 115 416 1245 Fragments arranged by size: 1245 416 115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1518 1552 1776 Size: 1518 34 224 Fragments arranged by size: 1518 224 34 BciVI GTATCCnnnnn_n' Cuts at: 0 713 1776 Size: 713 1063 BglII A'GATC_T Cuts at: 0 1328 1776 Size: 1328 448 BmrI ACTGGGnnnn_n' Cuts at: 0 53 650 1031 1776 Size: 53 597 381 745 Fragments arranged by size: 745 597 381 53 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1707 1734 1776 Size: 1707 27 42 Fragments arranged by size: 1707 42 27 Bpu10I CC'TnA_GC Cuts at: 0 1195 1776 Size: 1195 581 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 982 1776 Size: 982 794 BsaAI yAC'GTr Cuts at: 0 377 910 1776 Size: 377 533 866 Fragments arranged by size: 866 533 377 BsaBI GATnn'nnATC Cuts at: 0 875 956 1776 Size: 875 81 820 Fragments arranged by size: 875 820 81 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 201 231 275 305 1655 1685 1776 Size: 201 30 44 30 1350 30 91 Fragments arranged by size: 1350 201 91 44 30 30 30 BseYI C'CCAG_C Cuts at: 0 1518 1776 Size: 1518 258 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1732 1776 Size: 1732 44 BsiWI C'GTAC_G Cuts at: 0 398 1776 Size: 398 1378 BsmI GAATG_Cn' Cuts at: 0 356 1776 Size: 356 1420 BstAPI GCAn_nnn'nTGC Cuts at: 0 1243 1704 1776 Size: 1243 461 72 Fragments arranged by size: 1243 461 72 BstYI r'GATC_y Cuts at: 0 12 813 1050 1328 1718 1776 Size: 12 801 237 278 390 58 Fragments arranged by size: 801 390 278 237 58 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1717 1776 Size: 1717 59 BtsI GCAGTG_nn' Cuts at: 0 153 1776 Size: 153 1623 ClaI AT'CG_AT Cuts at: 0 879 1776 Size: 879 897 DraI TTT'AAA Cuts at: 0 182 1776 Size: 182 1594 EaeI y'GGCC_r Cuts at: 0 663 1776 Size: 663 1113 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 464 492 1046 1157 1707 1734 1776 Size: 464 28 554 111 550 27 42 Fragments arranged by size: 554 550 464 111 42 28 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 1713 1776 Size: 1713 63 EcoO109I rG'GnC_Cy Cuts at: 0 1025 1191 1776 Size: 1025 166 585 Fragments arranged by size: 1025 585 166 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1320 1776 Size: 1320 456 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 631 663 889 921 959 991 1776 Size: 631 32 226 32 38 32 785 Fragments arranged by size: 785 631 226 38 32 32 32 HincII GTy'rAC Cuts at: 0 1436 1776 Size: 1436 340 HindIII A'AGCT_T Cuts at: 0 504 1776 Size: 504 1272 MfeI C'AATT_G Cuts at: 0 203 1776 Size: 203 1573 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 293 1335 1776 Size: 293 1042 441 Fragments arranged by size: 1042 441 293 MscI TGG'CCA Cuts at: 0 665 1776 Size: 665 1111 MslI CAynn'nnrTG Cuts at: 0 349 1160 1776 Size: 349 811 616 Fragments arranged by size: 811 616 349 NdeI CA'TA_TG Cuts at: 0 324 414 1776 Size: 324 90 1362 Fragments arranged by size: 1362 324 90 PflMI CCAn_nnn'nTGG Cuts at: 0 1758 1776 Size: 1758 18 PfoI T'CCnGG_A Cuts at: 0 1471 1776 Size: 1471 305 PmlI CAC'GTG Cuts at: 0 910 1776 Size: 910 866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 955 987 1776 Size: 955 32 789 Fragments arranged by size: 955 789 32 PpuMI rG'GwC_Cy Cuts at: 0 1025 1191 1776 Size: 1025 166 585 Fragments arranged by size: 1025 585 166 PsiI TTA'TAA Cuts at: 0 689 1776 Size: 689 1087 PstI C_TGCA'G Cuts at: 0 1236 1397 1776 Size: 1236 161 379 Fragments arranged by size: 1236 379 161 SfcI C'TryA_G Cuts at: 0 362 1232 1311 1393 1776 Size: 362 870 79 82 383 Fragments arranged by size: 870 383 362 82 79 SmlI C'TyrA_G Cuts at: 0 961 1776 Size: 961 815 SspI AAT'ATT Cuts at: 0 1491 1575 1776 Size: 1491 84 201 Fragments arranged by size: 1491 201 84 StyI C'CwwG_G Cuts at: 0 1071 1757 1776 Size: 1071 686 19 Fragments arranged by size: 1071 686 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 870 1071 1438 1776 Size: 870 201 367 338 Fragments arranged by size: 870 367 338 201 TatI w'GTAC_w Cuts at: 0 827 1776 Size: 827 949 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 275 307 894 926 1467 1499 1628 1660 Size: 275 32 587 32 541 32 129 32 Cuts at: 1660 1776 Size: 116 Fragments arranged by size: 587 541 275 129 116 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1150 1776 Size: 1150 626 XmnI GAAnn'nnTTC Cuts at: 0 597 1776 Size: 597 1179 Enzymes that do cut: AcuI AhdI AlwNI ApoI BaeI BamHI BanI BcgI BciVI BglII BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaXI BseYI BsgI BsiWI BsmI BstAPI BstYI BtgZI BtsI ClaI DraI EaeI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII MfeI MmeI MscI MslI NdeI PflMI PfoI PmlI PpiI PpuMI PsiI PstI SfcI SmlI SspI StyI TaqII TatI TstI Tth111I XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BanII BbeI BbsI BbvCI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BsaI BsaHI BsaWI BseRI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI CspCI DraIII DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MluI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI XcmI XhoI XmaI ZraI