################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AocI 1348 CCTCAGG CC^T[ACGT]AGG AseI 468 ATTAAT AT^TAAT AsnI 468 ATTAAT AT^TAAT Asp700I 164 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 866 GTGCTC G[AT]GC[AT]^C AvaII 923 GGTCC G^G[AT]CC BbrPI 1249 CACGTG CAC^GTG BclI 90 TGATCA T^GATCA BlpI 651 GCTGAGC GC^T[ACGT]AGC BmyI 866 GTGCTC G[AGT]GC[ACT]^C BpmI 777 CTGGAG CTGGAG(16/14) Bpu1102I 651 GCTGAGC GC^T[ACGT]AGC BsiEI 1128 CGATCG CG[GA][CT]^CG BsiHKAI 866 GTGCTC G[AT]GC[AT]^C Bsp1286I 866 GTGCTC G[AGT]GC[ACT]^C BspHI 675 TCATGA T^CATGA Bsu36I 1348 CCTCAGG CC^T[ACGT]AGG CelII 651 GCTGAGC GC^T[ACGT]AGC DraII 922 AGGTCCC [GA]G^G[ACGT]CC[CT] EarI 164 GAAGAG CTCTTC(1/4) Eco81I 1348 CCTCAGG CC^T[ACGT]AGG EcoNI 53 CCTCTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 922 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 1069 GAATTC G^AATTC FbaI 90 TGATCA T^GATCA Ksp632I 164 GAAGAG CTCTTC(1/4) MwoI 562 GCAGTCCACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflMI 726 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1116 GAGTC GAGTC(4/5) PmaCI 1249 CACGTG CAC^GTG PmlI 1249 CACGTG CAC^GTG PpuMI 922 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 886 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 468 ATTAAT AT^TAAT PvuI 1128 CGATCG CGAT^CG RcaI 675 TCATGA T^CATGA SfaNI 1315 GATGC GCATC(5/9) TaqI 1297 TCGA T^CGA Tsp45I 1133 GTCAC ^GT[GC]AC TthHB8I 1297 TCGA T^CGA Van91I 726 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 164 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AciI 304 GCGG CCGC(-3/-1) 574 GCGG AflIII 129 ACGTGT A^C[GA][CT]GT 1248 ACACGT AlwI 47 GGATC GGATC(4/5) 684 GGATC BbsI 955 GAAGAC GAAGAC(2/6) 458 GTCTTC BglII 277 AGATCT A^GATCT 781 AGATCT BpuAI 955 GAAGAC GAAGAC(2/6) 458 GTCTTC BsaAI 128 TACGTG [CT]AC^GT[GA] 1249 CACGTG BsaBI 149 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 679 GATCTGGATC BsaJI 29 CCTTGG C^C[ACGT][ACGT]GG 637 CCTTGG BsmAI 853 GAGAC GTCTC(1/5) 229 GTCTC BsmFI 1278 GGGAC GGGAC(10/14) 924 GTCCC BsrI 762 ACTGG ACTGG(1/-1) 776 ACTGG BssKI 691 CCAGG ^CC[ACGT]GG 1208 CCAGG BstNI 691 CCAGG CC^[AT]GG 1208 CCAGG BstYI 277 AGATCT [GA]^GATC[CT] 781 AGATCT Cfr13I 923 GGTCC G^G[ACGT]CC 1205 GGGCC EcoRII 691 CCAGG ^CC[AT]GG 1208 CCAGG EcoT14I 29 CCTTGG C^C[AT][AT]GG 637 CCTTGG HindIII 435 AAGCTT A^AGCTT 616 AAGCTT MamI 149 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 679 GATCTGGATC MflI 277 AGATCT [GA]^GATC[CT] 781 AGATCT MspA1I 665 CAGCTG C[AC]G^C[GT]G 1269 CAGCTG MvaI 691 CCAGG CC^[AT]GG 1208 CCAGG PstI 785 CTGCAG CTGCA^G 1282 CTGCAG PvuII 665 CAGCTG CAG^CTG 1269 CAGCTG Sau96I 923 GGTCC G^G[ACGT]CC 1205 GGGCC ScaI 476 AGTACT AGT^ACT 759 AGTACT ScrFI 691 CCAGG CC^[ACGT]GG 1208 CCAGG SfcI 785 CTGCAG C^T[GA][CT]AG 1282 CTGCAG StyI 29 CCTTGG C^C[AT][AT]GG 637 CCTTGG XhoII 277 AGATCT [GA]^GATC[CT] 781 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BfaI BsiYI BslI BstF5I Cac8I DdeI DpnII EcoRV Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI NdeII NlaIII NlaIV RsaI Sau3AI TfiI Tru9I TseI Tsp509I TspRI XcmI