(Circular) (Six-base) MAPSORT of: pFN21AE2619.seq Check: 7524 from: 1 to: 6073 pFN21AE2619 6073 bp With 182 enzymes: SgfI * April 15, 2016 11:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3883 278 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3416 1937 Size: 1479 4594 Acc65I G'GTAC_C Cuts at: 3394 3394 Size: 6073 AclI AA'CG_TT Cuts at: 1563 4200 4573 1563 Size: 2637 373 3063 Fragments arranged by size: 3063 2637 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4136 5520 5788 1632 Size: 2504 1384 268 1917 Fragments arranged by size: 2504 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5876 AflIII A'CryG_T Cuts at: 1203 2134 3253 4972 5949 1203 Size: 931 1119 1719 977 1327 Fragments arranged by size: 1719 1327 1119 977 931 AgeI A'CCGG_T Cuts at: 4904 4904 Size: 6073 AhdI GACnn_n'nnGTC Cuts at: 4802 4802 Size: 6073 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6073 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6073 AlwNI CAG_nnn'CTG Cuts at: 3816 5388 3816 Size: 1572 4501 ApaLI G'TGCA_C Cuts at: 4130 5286 4130 Size: 1156 4917 ApoI r'AATT_y Cuts at: 1493 2317 2389 3074 3382 3608 3760 5869 1493 Size: 824 72 685 308 226 152 2109 1697 Fragments arranged by size: 2109 1697 824 685 308 226 152 72 AseI AT'TA_AT Cuts at: 160 1032 2474 4627 160 Size: 872 1442 2153 1606 Fragments arranged by size: 2153 1606 1442 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 AvaI C'yCGr_G Cuts at: 1870 1943 3398 1870 Size: 73 1455 4545 Fragments arranged by size: 4545 1455 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2295 2328 2295 Size: 33 6040 BamHI G'GATC_C Cuts at: 3403 3770 3403 Size: 367 5706 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3394 4849 618 Size: 325 217 627 1607 1455 1842 Fragments arranged by size: 1842 1607 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3392 1391 Size: 2001 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2456 2967 928 Size: 971 557 511 4034 Fragments arranged by size: 4034 971 557 511 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4264 4298 1903 Size: 34 2327 34 3678 Fragments arranged by size: 3678 2327 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3965 5175 3965 Size: 1210 4863 BclI T'GATC_A Cuts at: 1548 2095 1548 Size: 547 5526 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4684 136 Size: 107 122 71 631 605 3012 1525 Fragments arranged by size: 3012 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2282 2786 6068 2282 Size: 504 3282 2287 Fragments arranged by size: 3282 2287 504 BlpI GC'TnA_GC Cuts at: 2657 3490 2657 Size: 833 5240 Bme1580I G_kGCm'C Cuts at: 1790 4134 5290 1790 Size: 2344 1156 2573 Fragments arranged by size: 2573 2344 1156 BmgBI CAC'GTC Cuts at: 1318 3033 1318 Size: 1715 4358 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3804 4762 442 Size: 416 53 83 399 24 2387 958 1753 Fragments arranged by size: 2387 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6073 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3355 3355 Size: 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2803 4733 1239 Size: 1564 1930 2579 Fragments arranged by size: 2579 1930 1564 Bpu10I CC'TnA_GC Cuts at: 2619 2619 Size: 6073 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4198 5063 5361 4198 Size: 865 298 4910 Fragments arranged by size: 4910 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4736 882 Size: 617 3237 2219 Fragments arranged by size: 3237 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2135 3256 3864 493 Size: 711 931 1121 608 2702 Fragments arranged by size: 2702 1121 931 711 608 BsaBI GATnn'nnATC Cuts at: 2158 2688 6067 2158 Size: 530 3379 2164 Fragments arranged by size: 3379 2164 530 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3880 4262 4897 275 Size: 53 83 186 936 2347 382 635 1451 Fragments arranged by size: 2347 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4506 4904 5178 5325 5736 4506 Size: 398 274 147 411 4843 Fragments arranged by size: 4843 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5848 Fragments arranged by size: 5848 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6073 BseYI C'CCAG_C Cuts at: 5276 5772 5276 Size: 496 5577 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5500 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3136 3523 4284 5312 1540 Size: 275 186 1135 387 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 387 275 186 BsiHKAI G_wGCw'C Cuts at: 2875 4134 4219 5290 2875 Size: 1259 85 1071 3658 Fragments arranged by size: 3658 1259 1071 85 BsmI GAATG_Cn' Cuts at: 3584 3677 3584 Size: 93 5980 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2875 3392 4134 4219 5290 1391 Size: 399 1085 517 742 85 1071 2174 Fragments arranged by size: 2174 1085 1071 742 517 399 85 BspEI T'CCGG_A Cuts at: 5736 5736 Size: 6073 BspHI T'CATG_A Cuts at: 2680 3962 5692 2680 Size: 1282 1730 3061 Fragments arranged by size: 3061 1730 1282 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3428 844 Size: 2584 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3960 1394 Size: 429 2137 3507 Fragments arranged by size: 3507 2137 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4568 4742 65 Size: 1183 3320 174 1396 Fragments arranged by size: 3320 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4717 4904 1700 Size: 110 2907 187 2869 Fragments arranged by size: 2907 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6073 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6073 BssSI C'ACGA_G Cuts at: 4133 5145 4133 Size: 1012 5061 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6073 BstBI TT'CG_AA Cuts at: 4877 4877 Size: 6073 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6073 BstYI r'GATC_y Cuts at: 1972 2282 2786 3403 3770 4155 4172 5624 5710 Size: 310 504 617 367 385 17 1452 86 Cuts at: 5710 5722 6068 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 617 504 385 367 346 310 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3354 3354 Size: 6073 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4582 Fragments arranged by size: 4582 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5334 Fragments arranged by size: 5334 683 56 BtsI GCAGTG_nn' Cuts at: 2799 3056 3313 3589 4383 4403 2799 Size: 257 257 276 794 20 4469 Fragments arranged by size: 4469 794 276 257 257 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6073 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6038 DraI TTT'AAA Cuts at: 3377 3377 Size: 6073 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5080 809 Size: 507 3764 1802 Fragments arranged by size: 3764 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3520 4409 6038 Size: 54 1425 50 275 18 1690 889 1629 Cuts at: 6038 8 Size: 43 Fragments arranged by size: 1690 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3520 1537 Size: 275 1708 4090 Fragments arranged by size: 4090 1708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2164 4003 1353 Size: 811 1839 3423 Fragments arranged by size: 3423 1839 811 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4660 5034 5180 387 Size: 1064 138 3071 374 146 1280 Fragments arranged by size: 3071 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2803 4136 4733 5520 5788 1239 Size: 393 1171 1333 597 787 268 1524 Fragments arranged by size: 1524 1333 1171 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2062 2062 Size: 6073 EcoO109I rG'GnC_Cy Cuts at: 2928 2928 Size: 6073 EcoRI G'AATT_C Cuts at: 3074 3382 3760 3074 Size: 308 378 5387 Fragments arranged by size: 5387 378 308 EcoRV GAT'ATC Cuts at: 2276 2398 2650 2276 Size: 122 252 5699 Fragments arranged by size: 5699 252 122 FspI TGC'GCA Cuts at: 4579 4579 Size: 6073 HaeII r_GCGC'y Cuts at: 1678 5220 1678 Size: 3542 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2011 2043 2857 2889 2900 Size: 32 135 32 439 32 814 32 11 Cuts at: 2900 2932 4714 4746 4788 4820 1373 Size: 32 1782 32 42 32 2626 Fragments arranged by size: 2626 1782 814 439 135 42 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 3417 3663 669 Size: 988 281 1479 246 3079 Fragments arranged by size: 3079 1479 988 281 246 HindIII A'AGCT_T Cuts at: 748 2440 2621 748 Size: 1692 181 4200 Fragments arranged by size: 4200 1692 181 HpaI GTT'AAC Cuts at: 3663 3663 Size: 6073 KpnI G_GTAC'C Cuts at: 3398 3398 Size: 6073 MfeI C'AATT_G Cuts at: 3672 3672 Size: 6073 MluI A'CGCG_T Cuts at: 5949 5949 Size: 6073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2359 3008 5187 5371 1305 Size: 1054 649 2179 184 2007 Fragments arranged by size: 2179 2007 1054 649 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4251 Fragments arranged by size: 4251 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 2697 3114 4031 4390 4549 518 Size: 648 951 580 417 917 359 159 2042 Fragments arranged by size: 2042 951 917 648 580 417 359 159 MspA1I CmG'CkG Cuts at: 1606 2672 3276 3489 4166 5314 5559 1606 Size: 1066 604 213 677 1148 245 2120 Fragments arranged by size: 2120 1148 1066 677 604 245 213 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4582 Fragments arranged by size: 4582 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6073 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6073 NotI GC'GGCC_GC Cuts at: 3520 3520 Size: 6073 NspI r_CATG'y Cuts at: 1132 1229 3431 4976 1132 Size: 97 2202 1545 2229 Fragments arranged by size: 2229 2202 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6073 PciI A'CATG_T Cuts at: 4972 4972 Size: 6073 PflMI CCAn_nnn'nTGG Cuts at: 1108 2737 1108 Size: 1629 4444 PmeI GTTT'AAAC Cuts at: 3377 3377 Size: 6073 PmlI CAC'GTG Cuts at: 3256 3256 Size: 6073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4142 4174 5655 5687 4142 Size: 32 1481 32 4528 Fragments arranged by size: 4528 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2928 2928 Size: 6073 PshAI GACnn'nnGTC Cuts at: 1613 2895 1613 Size: 1282 4791 PsiI TTA'TAA Cuts at: 2346 3643 2346 Size: 1297 4776 PstI C_TGCA'G Cuts at: 830 2794 3291 3425 830 Size: 1964 497 134 3478 Fragments arranged by size: 3478 1964 497 134 PvuI CG_AT'CG Cuts at: 2001 3136 2001 Size: 1135 4938 PvuII CAG'CTG Cuts at: 2672 3276 2672 Size: 604 5469 SalI G'TCGA_C Cuts at: 1936 3415 1936 Size: 1479 4594 SbfI CC_TGCA'GG Cuts at: 3425 3425 Size: 6073 ScaI AGT'ACT Cuts at: 2483 2766 4321 6033 2483 Size: 283 1555 1712 2523 Fragments arranged by size: 2523 1712 1555 283 SfcI C'TryA_G Cuts at: 826 1045 2790 3287 3421 4556 5237 5428 826 Size: 219 1745 497 134 1135 681 191 1471 Fragments arranged by size: 1745 1471 1135 681 497 219 191 134 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6073 SmaI CCC'GGG Cuts at: 1872 3400 1872 Size: 1528 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4177 5078 5340 820 Size: 197 926 2234 901 262 1553 Fragments arranged by size: 2234 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3864 493 Size: 3371 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6073 SphI G_CATG'C Cuts at: 1132 3431 1132 Size: 2299 3774 SspI AAT'ATT Cuts at: 5 52 3997 5 Size: 47 3945 2081 Fragments arranged by size: 3945 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6073 StyI C'CwwG_G Cuts at: 513 1065 2004 2034 2642 3512 513 Size: 552 939 30 608 870 3074 Fragments arranged by size: 3074 939 870 608 552 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2196 3104 4100 4117 4270 4455 4900 Size: 1255 616 908 996 17 153 185 445 Cuts at: 4900 325 Size: 1498 Fragments arranged by size: 1498 1255 996 908 616 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2481 2764 4319 Size: 274 80 33 51 1443 504 283 1555 Cuts at: 4319 6031 96 Size: 1712 138 Fragments arranged by size: 1712 1555 1443 504 283 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6033 6065 6033 Size: 32 6041 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6073 XbaI T'CTAG_A Cuts at: 3409 3409 Size: 6073 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2365 2683 3030 3245 2365 Size: 318 347 215 5193 Fragments arranged by size: 5193 347 318 215 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6073 XmaI C'CCGG_G Cuts at: 1870 3398 1870 Size: 1528 4545 XmnI GAAnn'nnTTC Cuts at: 2173 4202 2173 Size: 2029 4044 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3881 276 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI