Restriction map for
pFN21AE5734
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AclI AcyI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BlnI BsaAI BsaHI BsgI BsiEI
BsiWI BsmI BsoBI BspLU11I BspMI
BsrFI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I CfoI Cfr10I CpoI
DraI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MscI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 551 GTAGAC GT^[AC][GT]AC
AccII 410 CGCG CG^CG
AccIII 545 TCCGGA T^CCGGA
AflIII 275 ACGTGT A^C[GA][CT]GT
AhdI 1864 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1840 GATCC GGATC(4/5)
AlwNI 619 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 253 CCTCAGG CC^T[ACGT]AGG
AseI 1104 ATTAAT AT^TAAT
AsnI 1104 ATTAAT AT^TAAT
AspEI 1864 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 644 GGTGCC G^G[CT][GA]CC
BbsI 778 GAAGAC GAAGAC(2/6)
BclI 1431 TGATCA T^GATCA
BglII 1845 AGATCT A^GATCT
BlpI 441 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 441 GCTCAGC GC^T[ACGT]AGC
BpuAI 778 GAAGAC GAAGAC(2/6)
BsaBI 1840 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1898 GGTCTC GGTCTC(1/5)
BseAI 545 TCCGGA T^CCGGA
BseRI 319 GAGGAG GAGGAG(10/8)
BsmBI 1338 GAGACG CGTCTC(1/5)
BsmFI 1025 GGGAC GGGAC(10/14)
BspDI 388 ATCGAT AT^CGAT
BspEI 545 TCCGGA T^CCGGA
BspHI 833 TCATGA T^CATGA
BsrBI 1034 CCGCTC CCGCTC(-3/-3)
BstEII 1168 GGTGACC G^GT[ACGT]ACC
BstPI 1168 GGTGACC G^GT[ACGT]ACC
BstUI 410 CGCG CG^CG
Bsu36I 253 CCTCAGG CC^T[ACGT]AGG
CelII 441 GCTCAGC GC^T[ACGT]AGC
ClaI 388 ATCGAT AT^CGAT
DraII 1607 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 460 GACTATAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 512 CCACGG C^C[GA][CT]GG
EaeI 509 CGGCCA [CT]^GGCC[GA]
Eam1105I 1864 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1665 GAAGAG CTCTTC(1/4)
Eco81I 253 CCTCAGG CC^T[ACGT]AGG
EcoNI 1780 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1607 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1168 GGTGACC G^GT[ACGT]ACC
EcoT14I 20 CCAAGG C^C[AT][AT]GG
FbaI 1431 TGATCA T^GATCA
HgaI 127 GACGC GACGC(5/10)
HincII 715 GTTGAC GT[CT]^[GA]AC
HindII 715 GTTGAC GT[CT]^[GA]AC
Ksp632I 1665 GAAGAG CTCTTC(1/4)
MamI 1840 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 545 TCCGGA T^CCGGA
MvnI 410 CGCG CG^CG
NspI 1917 GCATGC [GA]CATG^[CT]
PflMI 1903 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1104 ATTAAT AT^TAAT
RcaI 833 TCATGA T^CATGA
SmlI 1348 CTCAAG C^T[CT][GA]AG
SphI 1917 GCATGC GCATG^C
StyI 20 CCAAGG C^C[AT][AT]GG
Van91I 1903 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1161 TCTAGA T^CTAGA
XcmI 1737 CCAGGATTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AspHI 4 GAGCAC G[AT]GC[AT]^C
1044 GTGCTC
BsiHKAI 4 GAGCAC G[AT]GC[AT]^C
1044 GTGCTC
Bsp1407I 278 TGTACA T^GTACA
1487 TGTACA
BsrDI 966 CATTGC GCAATG(2/0)
739 GCAATG
BsrGI 278 TGTACA T^GTACA
1487 TGTACA
BstAPI 745 GCAAATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
849 GCAGAAAATGC
BstYI 1839 AGATCC [GA]^GATC[CT]
1845 AGATCT
DraIII 271 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
1922 CACCGAGTG
MaeII 275 ACGT A^CGT
497 ACGT
MflI 1839 AGATCC [GA]^GATC[CT]
1845 AGATCT
PstI 755 CTGCAG CTGCA^G
847 CTGCAG
SspBI 278 TGTACA T^GTACA
1487 TGTACA
TaqI 48 TCGA T^CGA
389 TCGA
TthHB8I 48 TCGA T^CGA
389 TCGA
XhoII 1839 AGATCC [GA]^GATC[CT]
1845 AGATCT
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsaWI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XmnI