(Circular) (Six-base) MAPSORT of: pFN21AE5734.seq Check: 7522 from: 1 to: 6685 pFN21AE5734 6685 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4495 278 Size: 53 83 186 936 2959 2468 Fragments arranged by size: 2959 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2557 4028 1937 Size: 620 1471 4594 Fragments arranged by size: 4594 1471 620 Acc65I G'GTAC_C Cuts at: 4006 4006 Size: 6685 AclI AA'CG_TT Cuts at: 1563 4812 5185 1563 Size: 3249 373 3063 Fragments arranged by size: 3249 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2623 3113 4748 6132 6400 1632 Size: 991 490 1635 1384 268 1917 Fragments arranged by size: 1917 1635 1384 991 490 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6488 AflIII A'CryG_T Cuts at: 1203 2280 5584 6561 1203 Size: 1077 3304 977 1327 Fragments arranged by size: 3304 1327 1077 977 AgeI A'CCGG_T Cuts at: 5516 5516 Size: 6685 AhdI GACnn_n'nnGTC Cuts at: 3874 5414 3874 Size: 1540 5145 AleI CACnn'nnGTG Cuts at: 1166 3665 1166 Size: 2499 4186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6685 AlwNI CAG_nnn'CTG Cuts at: 2629 4428 6000 2629 Size: 1799 1572 3314 Fragments arranged by size: 3314 1799 1572 ApaLI G'TGCA_C Cuts at: 4742 5898 4742 Size: 1156 5529 ApoI r'AATT_y Cuts at: 1493 2122 2546 3636 3994 4220 4372 6481 1493 Size: 629 424 1090 358 226 152 2109 1697 Fragments arranged by size: 2109 1697 1090 629 424 358 226 152 AseI AT'TA_AT Cuts at: 160 1032 3110 5239 160 Size: 872 2078 2129 1606 Fragments arranged by size: 2129 2078 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 AvaI C'yCGr_G Cuts at: 1870 1943 4010 1870 Size: 73 2067 4545 Fragments arranged by size: 4545 2067 73 BamHI G'GATC_C Cuts at: 4015 4382 4015 Size: 367 6318 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2649 4006 5461 618 Size: 325 217 627 862 1357 1455 1842 Fragments arranged by size: 1842 1455 1357 862 627 325 217 BanII G_rGCy'C Cuts at: 1391 3388 3690 3785 4004 1391 Size: 1997 302 95 219 4072 Fragments arranged by size: 4072 1997 302 219 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2790 928 Size: 971 891 4823 Fragments arranged by size: 4823 971 891 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4876 4910 1903 Size: 34 2939 34 3678 Fragments arranged by size: 3678 2939 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4577 5787 4577 Size: 1210 5475 BclI T'GATC_A Cuts at: 1548 3436 1548 Size: 1888 4797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5296 136 Size: 107 122 71 631 605 3624 1525 Fragments arranged by size: 3624 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3850 6680 3850 Size: 2830 3855 BlpI GC'TnA_GC Cuts at: 2447 4102 2447 Size: 1655 5030 Bme1580I G_kGCm'C Cuts at: 1790 2647 4746 5902 1790 Size: 857 2099 1156 2573 Fragments arranged by size: 2573 2099 1156 857 BmgBI CAC'GTC Cuts at: 1318 2281 1318 Size: 963 5722 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4416 5374 442 Size: 416 53 83 399 24 2999 958 1753 Fragments arranged by size: 2999 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6685 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2657 2936 3177 5345 1239 Size: 1418 279 241 2168 2579 Fragments arranged by size: 2579 2168 1418 279 241 Bpu10I CC'TnA_GC Cuts at: 3042 3042 Size: 6685 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3338 4810 5675 5973 3338 Size: 1472 865 298 4050 Fragments arranged by size: 4050 1472 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3909 5348 882 Size: 617 2410 1439 2219 Fragments arranged by size: 2410 2219 1439 617 BsaAI yAC'GTr Cuts at: 493 1204 4476 493 Size: 711 3272 2702 Fragments arranged by size: 3272 2702 711 BsaBI GATnn'nnATC Cuts at: 3849 6679 3849 Size: 2830 3855 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4492 4874 5509 275 Size: 53 83 186 936 2959 382 635 1451 Fragments arranged by size: 2959 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2201 2550 3084 5118 5516 5790 5937 6348 2201 Size: 349 534 2034 398 274 147 411 2538 Fragments arranged by size: 2538 2034 534 411 398 349 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2588 2618 1178 Size: 30 165 30 1185 30 5245 Fragments arranged by size: 5245 1185 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2339 1186 Size: 1153 5532 BseYI C'CCAG_C Cuts at: 2382 3027 3689 5888 6384 2382 Size: 645 662 2199 496 2683 Fragments arranged by size: 2683 2199 662 645 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6112 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4135 4896 5924 1540 Size: 275 186 2134 761 1028 2301 Fragments arranged by size: 2301 2134 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2013 3053 4746 4831 5902 2013 Size: 1040 1693 85 1071 2796 Fragments arranged by size: 2796 1693 1071 1040 85 BsmI GAATG_Cn' Cuts at: 4196 4289 4196 Size: 93 6592 BsmBI CGTCTCn'nnnn_ Cuts at: 3337 3337 Size: 6685 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2647 3053 3388 3690 3785 4004 Size: 399 223 634 406 335 302 95 219 Cuts at: 4004 4746 4831 5902 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 634 406 399 335 302 223 219 95 85 BspEI T'CCGG_A Cuts at: 2550 6348 2550 Size: 3798 2887 BspHI T'CATG_A Cuts at: 2838 4574 6304 2838 Size: 1736 1730 3219 Fragments arranged by size: 3219 1736 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4040 844 Size: 3196 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3041 4572 1394 Size: 429 1218 1531 3507 Fragments arranged by size: 3507 1531 1218 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2751 2970 5180 5354 65 Size: 1183 1503 219 2210 174 1396 Fragments arranged by size: 2210 1503 1396 1183 219 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5329 5516 1700 Size: 110 3519 187 2869 Fragments arranged by size: 3519 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2283 3492 96 Size: 2187 1209 3289 Fragments arranged by size: 3289 2187 1209 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6685 BssSI C'ACGA_G Cuts at: 4745 5757 4745 Size: 1012 5673 BstAPI GCAn_nnn'nTGC Cuts at: 794 2756 2860 794 Size: 1962 104 4619 Fragments arranged by size: 4619 1962 104 BstBI TT'CG_AA Cuts at: 5489 5489 Size: 6685 BstEII G'GTnAC_C Cuts at: 1184 3173 1184 Size: 1989 4696 BstYI r'GATC_y Cuts at: 1972 3844 3850 4015 4382 4767 4784 6236 6322 Size: 1872 6 165 367 385 17 1452 86 Cuts at: 6322 6334 6680 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1872 1452 385 367 346 165 86 17 12 6 Bsu36I CC'TnA_GG Cuts at: 2259 2259 Size: 6685 BtgI C'CryG_G Cuts at: 513 1065 2004 2517 513 Size: 552 939 513 4681 Fragments arranged by size: 4681 939 552 513 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2466 3810 487 Size: 683 56 1240 1344 3362 Fragments arranged by size: 3362 1344 1240 683 56 BtsI GCAGTG_nn' Cuts at: 2988 3579 3978 4201 4995 5015 2988 Size: 591 399 223 794 20 4658 Fragments arranged by size: 4658 794 591 399 223 20 ClaI AT'CG_AT Cuts at: 1554 2394 1554 Size: 840 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6650 DraI TTT'AAA Cuts at: 3989 3989 Size: 6685 DraIII CAC_nnn'GTG Cuts at: 2281 3932 2281 Size: 1651 5034 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2471 5692 809 Size: 507 1155 3221 1802 Fragments arranged by size: 3221 1802 1155 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2514 4132 5021 Size: 54 1425 50 275 18 684 1618 889 Cuts at: 5021 6650 8 Size: 1629 43 Fragments arranged by size: 1629 1618 1425 889 684 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4132 1537 Size: 275 2320 4090 Fragments arranged by size: 4090 2320 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3665 4615 1353 Size: 2312 950 3423 Fragments arranged by size: 3423 2312 950 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5272 5646 5792 387 Size: 1064 138 3683 374 146 1280 Fragments arranged by size: 3683 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2623 2657 2936 3113 3177 4748 5345 Size: 393 991 34 279 177 64 1571 597 Cuts at: 5345 6132 6400 1239 Size: 787 268 1524 Fragments arranged by size: 1571 1524 991 787 597 393 279 268 177 64 34 EcoNI CCTnn'n_nnAGG Cuts at: 3789 3789 Size: 6685 EcoO109I rG'GnC_Cy Cuts at: 3613 3613 Size: 6685 EcoRI G'AATT_C Cuts at: 3994 4372 3994 Size: 378 6307 FspI TGC'GCA Cuts at: 5191 5191 Size: 6685 HaeII r_GCGC'y Cuts at: 1678 5832 1678 Size: 4154 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 2556 2588 2833 Size: 32 135 32 470 32 482 32 245 Cuts at: 2833 2865 3336 3368 3860 3892 5326 5358 5400 Size: 32 471 32 492 32 1434 32 42 Cuts at: 5400 5432 1373 Size: 32 2626 Fragments arranged by size: 2626 1434 492 482 471 470 245 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2722 4029 4275 669 Size: 988 281 784 1307 246 3079 Fragments arranged by size: 3079 1307 988 784 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6685 HpaI GTT'AAC Cuts at: 4275 4275 Size: 6685 KpnI G_GTAC'C Cuts at: 4010 4010 Size: 6685 MfeI C'AATT_G Cuts at: 4284 4284 Size: 6685 MluI A'CGCG_T Cuts at: 6561 6561 Size: 6685 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5799 5983 1305 Size: 4494 184 2007 Fragments arranged by size: 4494 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4863 Fragments arranged by size: 4863 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 2417 2531 3156 3665 4643 5002 Size: 648 948 303 114 625 509 978 359 Cuts at: 5002 5161 518 Size: 159 2042 Fragments arranged by size: 2042 978 948 648 625 509 359 303 159 114 MspA1I CmG'CkG Cuts at: 1606 4101 4778 5926 6171 1606 Size: 2495 677 1148 245 2120 Fragments arranged by size: 2495 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5194 Fragments arranged by size: 5194 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6685 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6685 NotI GC'GGCC_GC Cuts at: 4132 4132 Size: 6685 NspI r_CATG'y Cuts at: 1132 1229 3926 4043 5588 1132 Size: 97 2697 117 1545 2229 Fragments arranged by size: 2697 2229 1545 117 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6685 PciI A'CATG_T Cuts at: 5584 5584 Size: 6685 PflMI CCAn_nnn'nTGG Cuts at: 1108 3914 1108 Size: 2806 3879 PfoI T'CCnGG_A Cuts at: 2695 3776 2695 Size: 1081 5604 PmeI GTTT'AAAC Cuts at: 3989 3989 Size: 6685 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4754 4786 6267 6299 4754 Size: 32 1481 32 5140 Fragments arranged by size: 5140 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6685 PsiI TTA'TAA Cuts at: 2589 4255 2589 Size: 1666 5019 PstI C_TGCA'G Cuts at: 830 2764 2856 4037 830 Size: 1934 92 1181 3478 Fragments arranged by size: 3478 1934 1181 92 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6685 SalI G'TCGA_C Cuts at: 1936 4027 1936 Size: 2091 4594 SbfI CC_TGCA'GG Cuts at: 4037 4037 Size: 6685 ScaI AGT'ACT Cuts at: 4933 6645 4933 Size: 1712 4973 SfcI C'TryA_G Cuts at: 826 1045 2467 2760 2852 4033 5168 5849 6040 Size: 219 1422 293 92 1181 1135 681 191 Cuts at: 6040 826 Size: 1471 Fragments arranged by size: 1471 1422 1181 1135 681 293 219 191 92 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6685 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6685 SmaI CCC'GGG Cuts at: 1872 4012 1872 Size: 2140 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3353 4789 5690 5952 820 Size: 197 926 1410 1436 901 262 1553 Fragments arranged by size: 1553 1436 1410 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4476 493 Size: 3983 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6685 SphI G_CATG'C Cuts at: 1132 3926 4043 1132 Size: 2794 117 3774 Fragments arranged by size: 3774 2794 117 SspI AAT'ATT Cuts at: 5 52 4609 5 Size: 47 4557 2081 Fragments arranged by size: 4557 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6685 StyI C'CwwG_G Cuts at: 513 1065 2004 2025 4124 513 Size: 552 939 21 2099 3074 Fragments arranged by size: 3074 2099 939 552 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2374 3891 4712 4729 4882 5067 5512 Size: 1255 794 1517 821 17 153 185 445 Cuts at: 5512 325 Size: 1498 Fragments arranged by size: 1517 1498 1255 821 794 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 3492 4931 Size: 274 80 33 51 1443 306 1209 1439 Cuts at: 4931 6643 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1439 1209 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3284 3316 6645 6677 3284 Size: 32 3329 32 3292 Fragments arranged by size: 3329 3292 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6685 XbaI T'CTAG_A Cuts at: 3166 4021 3166 Size: 855 5830 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3749 3749 Size: 6685 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6685 XmaI C'CCGG_G Cuts at: 1870 4010 1870 Size: 2140 4545 XmnI GAAnn'nnTTC Cuts at: 3098 3240 3636 4814 3098 Size: 142 396 1178 4969 Fragments arranged by size: 4969 1178 396 142 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4493 276 Size: 53 83 186 936 2959 2468 Fragments arranged by size: 2959 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI