Restriction map for
pFN21AE6384
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlpI BpmI Bpu1102I BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI MwoI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TaqI Tth111I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 16 GCGG CCGC(-3/-1)
AflIII 752 ACGTGT A^C[GA][CT]GT
AlwI 290 GATCC GGATC(4/5)
Asp700I 867 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 64 GAGCTC G[AT]GC[AT]^C
AvrII 948 CCTAGG C^CTAGG
BanI 895 GGCACC G^G[CT][GA]CC
BanII 64 GAGCTC G[GA]GC[CT]^C
BlnI 948 CCTAGG C^CTAGG
BsaAI 751 TACGTG [CT]AC^GT[GA]
BsaWI 292 TCCGGT [AT]^CCGG[AT]
BseRI 727 CTCCTC GAGGAG(10/8)
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
BsmAI 544 GAGAC GTCTC(1/5)
BsmI 787 GAATGC GAATGC(1/-1)
Bsp1407I 926 TGTACA T^GTACA
BspHI 731 TCATGA T^CATGA
BspMI 420 GCAGGT ACCTGC(4/8)
BsrGI 926 TGTACA T^GTACA
BstEII 207 GGTGACC G^GT[ACGT]ACC
BstPI 207 GGTGACC G^GT[ACGT]ACC
BstXI 368 CCATTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 289 AGATCC [GA]^GATC[CT]
Cac8I 34 GCAGGC GC[ACGT]^[ACGT]GC
DraII 916 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 876 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 426 GACTTCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 782 CCACGG C^C[GA][CT]GG
EarI 509 CTCTTC CTCTTC(1/4)
EcoO109I 916 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 207 GGTGACC G^GT[ACGT]ACC
Ksp632I 509 CTCTTC CTCTTC(1/4)
MflI 289 AGATCC [GA]^GATC[CT]
MseI 922 TTAA T^TAA
NdeI 277 CATATG CA^TATG
PleI 546 GACTC GAGTC(4/5)
PpuMI 916 GGGACCT [GA]G^G[AT]CC[CT]
PstI 42 CTGCAG CTGCA^G
RcaI 731 TCATGA T^CATGA
SacI 64 GAGCTC GAGCT^C
SapI 508 GCTCTTC GCTCTTC(1/4)
SfcI 42 CTGCAG C^T[GA][CT]AG
SmlI 441 CTTGAG C^T[CT][GA]AG
SspBI 926 TGTACA T^GTACA
Tru9I 922 TTAA T^TAA
TspRI 886 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 289 AGATCC [GA]^GATC[CT]
XmnI 867 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 683 AAATTC [GA]^AATT[CT]
829 AAATTT
ApoI 683 AAATTC [GA]^AATT[CT]
829 AAATTT
AvaII 917 GGACC G^G[AT]CC
953 GGACC
BbsI 622 GTCTTC GAAGAC(2/6)
907 GTCTTC
BbvI 38 GCTGC GCAGC(8/12)
41 GCTGC
BmyI 64 GAGCTC G[AGT]GC[ACT]^C
894 GGGCAC
BpuAI 622 GTCTTC GAAGAC(2/6)
907 GTCTTC
BsmFI 916 GGGAC GGGAC(10/14)
952 GGGAC
Bsp1286I 64 GAGCTC G[AGT]GC[ACT]^C
894 GGGCAC
BsrDI 261 CATTGC GCAATG(2/0)
369 CATTGC
BsrI 887 ACTGG ACTGG(1/-1)
432 CCAGT
CfoI 3 GCGC GCG^C
791 GCGC
DpnII 54 GATC ^GATC
290 GATC
Fnu4HI 38 GCTGC GC^[ACGT]GC
41 GCTGC
HhaI 3 GCGC GCG^C
791 GCGC
HinP1I 3 GCGC G^CGC
791 GCGC
ItaI 38 GCTGC GC^[ACGT]GC
41 GCTGC
MaeII 50 ACGT A^CGT
752 ACGT
MboI 54 GATC ^GATC
290 GATC
NdeII 54 GATC ^GATC
290 GATC
PflMI 85 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
710 CCATCATGTGG
Sau3AI 54 GATC ^GATC
290 GATC
SfaNI 81 GCATC GCATC(5/9)
758 GCATC
TseI 38 GCTGC G^C[AT]GC
41 GCTGC
Van91I 85 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
710 CCATCATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AfaI AluI BcnI BfaI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cfr13I DdeI EcoRII EcoT14I FokI
HaeIII HapII HinfI HpaII HphI
MaeI MaeIII MboII MnlI MslI
MspI MvaI NciI NlaIII NlaIV
RsaI Sau96I ScrFI StyI TfiI
Tsp45I Tsp509I