Restriction map for
pFN21AE2570
(Circular) (Six-base) MAPSORT of: pFN21AE2570.seq Check: 1418 from: 1 to: 6283
pFN21AE2570 6283 bp
With 182 enzymes: SgfI *
April 14, 2015 11:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4093 278
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3626 1937
Size: 1689 4594
Acc65I G'GTAC_C
Cuts at: 3604 3604
Size: 6283
AclI AA'CG_TT
Cuts at: 1563 4410 4783 1563
Size: 2847 373 3063
Fragments arranged by size:
3063 2847 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3212 3265 4346 5730 5998 1632
Size: 1580 53 1081 1384 268 1917
Fragments arranged by size:
1917 1580 1384 1081 268 53
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6086
AflIII A'CryG_T
Cuts at: 1203 5182 6159 1203
Size: 3979 977 1327
Fragments arranged by size:
3979 1327 977
AgeI A'CCGG_T
Cuts at: 5114 5114
Size: 6283
AhdI GACnn_n'nnGTC
Cuts at: 2748 5012 2748
Size: 2264 4019
AleI CACnn'nnGTG
Cuts at: 1166 2321 1166
Size: 1155 5128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6283
AlwNI CAG_nnn'CTG
Cuts at: 4026 5598 4026
Size: 1572 4711
ApaLI G'TGCA_C
Cuts at: 4340 5496 4340
Size: 1156 5127
ApoI r'AATT_y
Cuts at: 1493 2637 3592 3818 3970 6079 1493
Size: 1144 955 226 152 2109 1697
Fragments arranged by size:
2109 1697 1144 955 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4837 160
Size: 872 3805 1606
Fragments arranged by size:
3805 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
AvaI C'yCGr_G
Cuts at: 1870 1943 2614 2689 3608 1870
Size: 73 671 75 919 4545
Fragments arranged by size:
4545 919 671 75 73
BamHI G'GATC_C
Cuts at: 3613 3980 3613
Size: 367 5916
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3604 5059 618
Size: 325 217 627 1817 1455 1842
Fragments arranged by size:
1842 1817 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2372 2810 2855 3602 1391
Size: 981 438 45 747 4072
Fragments arranged by size:
4072 981 747 438 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3576 928
Size: 971 1677 3635
Fragments arranged by size:
3635 1677 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4474 4508 1903
Size: 34 50 34 2453 34 3678
Fragments arranged by size:
3678 2453 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2269 4175 5385 2269
Size: 1906 1210 3167
Fragments arranged by size:
3167 1906 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6283
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4894 136
Size: 107 122 71 631 605 3222 1525
Fragments arranged by size:
3222 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6278 6278
Size: 6283
BlpI GC'TnA_GC
Cuts at: 2292 3700 2292
Size: 1408 4875
Bme1580I G_kGCm'C
Cuts at: 1790 4344 5500 1790
Size: 2554 1156 2573
Fragments arranged by size:
2573 2554 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6283
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2303 2938 4014
Size: 416 53 83 399 24 886 635 1076
Cuts at: 4014 4972 442
Size: 958 1753
Fragments arranged by size:
1753 1076 958 886 635 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2133 2731 2839 4943 1239
Size: 894 598 108 2104 2579
Fragments arranged by size:
2579 2104 894 598 108
Bpu10I CC'TnA_GC
Cuts at: 2341 2341
Size: 6283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2969 4408 5273 5571 2969
Size: 1439 865 298 3681
Fragments arranged by size:
3681 1439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2837 3452 4946 882
Size: 617 1338 615 1494 2219
Fragments arranged by size:
2219 1494 1338 617 615
BsaAI yAC'GTr
Cuts at: 493 1204 4074 493
Size: 711 2870 2702
Fragments arranged by size:
2870 2702 711
BsaBI GATnn'nnATC
Cuts at: 6277 6277
Size: 6283
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4090 4472 5107 275
Size: 53 83 186 936 2557 382 635 1451
Fragments arranged by size:
2557 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3259 4716 5114 5388 5535 5946 3259
Size: 1457 398 274 147 411 3596
Fragments arranged by size:
3596 1457 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2887 2917 1178
Size: 30 165 30 1484 30 4544
Fragments arranged by size:
4544 1484 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2346 3278 3320 3416 3578 1186
Size: 1160 932 42 96 162 3891
Fragments arranged by size:
3891 1160 932 162 96 42
BseYI C'CCAG_C
Cuts at: 2187 3508 5486 5982 2187
Size: 1321 1978 496 2488
Fragments arranged by size:
2488 1978 1321 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2409 2816 3030 1163
Size: 573 673 407 214 4416
Fragments arranged by size:
4416 673 573 407 214
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3733 4494 5522 1540
Size: 275 186 1732 761 1028 2301
Fragments arranged by size:
2301 1732 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2198 2381 2855 4344 4429 5500 2198
Size: 183 474 1489 85 1071 2981
Fragments arranged by size:
2981 1489 1071 474 183 85
BsmI GAATG_Cn'
Cuts at: 2107 3794 3887 2107
Size: 1687 93 4503
Fragments arranged by size:
4503 1687 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2372 2381 2810 2855 3602 4344
Size: 399 408 174 9 429 45 747 742
Cuts at: 4344 4429 5500 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 747 742 429 408 399 174
85 45 9
BspEI T'CCGG_A
Cuts at: 5946 5946
Size: 6283
BspHI T'CATG_A
Cuts at: 2917 4172 5902 2917
Size: 1255 1730 3298
Fragments arranged by size:
3298 1730 1255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3638 844
Size: 2794 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3187 4170 1394
Size: 429 1364 983 3507
Fragments arranged by size:
3507 1364 983 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4778 4952 65
Size: 1183 3530 174 1396
Fragments arranged by size:
3530 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4927 5114 1700
Size: 110 3117 187 2869
Fragments arranged by size:
3117 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6283
BssHII G'CGCG_C
Cuts at: 1925 2013 1925
Size: 88 6195
BssSI C'ACGA_G
Cuts at: 2803 3332 4343 5355 2803
Size: 529 1011 1012 3731
Fragments arranged by size:
3731 1012 1011 529
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6283
BstBI TT'CG_AA
Cuts at: 5087 5087
Size: 6283
BstEII G'GTnAC_C
Cuts at: 1184 3425 1184
Size: 2241 4042
BstYI r'GATC_y
Cuts at: 1972 2944 3613 3980 4365 4382 5834 5920 5932
Size: 972 669 367 385 17 1452 86 12
Cuts at: 5932 6278 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 972 669 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2322 3129 513
Size: 552 1257 807 3667
Fragments arranged by size:
3667 1257 807 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5544
Fragments arranged by size:
5544 683 56
BtsI GCAGTG_nn'
Cuts at: 3799 4593 4613 3799
Size: 794 20 5469
Fragments arranged by size:
5469 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2386 2421 2640 2675 529
Size: 35 1822 35 219 35 4137
Fragments arranged by size:
4137 1822 219 35 35 35
DraI TTT'AAA
Cuts at: 3587 3587
Size: 6283
DraIII CAC_nnn'GTG
Cuts at: 2500 2966 2500
Size: 466 5817
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5290 809
Size: 507 3974 1802
Fragments arranged by size:
3974 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2598 3730 4619
Size: 54 1425 50 275 18 768 1132 889
Cuts at: 4619 6248 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1132 889 768 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3730 1537
Size: 275 1918 4090
Fragments arranged by size:
4090 1918 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3469 4213 1353
Size: 2116 744 3423
Fragments arranged by size:
3423 2116 744
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4870 5244 5390 387
Size: 1064 138 3281 374 146 1280
Fragments arranged by size:
3281 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2853 2853
Size: 6283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 2731 2839 3212 3265 4346 4943
Size: 393 501 598 108 373 53 1081 597
Cuts at: 4943 5730 5998 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1081 787 598 597 501 393 373
268 108 53
EcoNI CCTnn'n_nnAGG
Cuts at: 3545 3560 3545
Size: 15 6268
EcoRI G'AATT_C
Cuts at: 3592 3970 3592
Size: 378 5905
FspI TGC'GCA
Cuts at: 4789 4789
Size: 6283
HaeII r_GCGC'y
Cuts at: 1678 2024 2033 3021 3367 5430 1678
Size: 346 9 988 346 2063 2531
Fragments arranged by size:
2531 2063 988 346 346 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2501 2530 2533 2562 2734
Size: 32 135 32 929 29 3 29 172
Cuts at: 2734 2766 3514 3546 4924 4956 4998 5030 1373
Size: 32 748 32 1378 32 42 32 2626
Fragments arranged by size:
2626 1378 929 748 172 135 42 32
32 32 32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2049 2314 3627 3873 669
Size: 988 281 111 265 1313 246 3079
Fragments arranged by size:
3079 1313 988 281 265 246 111
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6283
HpaI GTT'AAC
Cuts at: 3873 3873
Size: 6283
KpnI G_GTAC'C
Cuts at: 3608 3608
Size: 6283
MfeI C'AATT_G
Cuts at: 3882 3882
Size: 6283
MluI A'CGCG_T
Cuts at: 6159 6159
Size: 6283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5397 5581 1305
Size: 4092 184 2007
Fragments arranged by size:
4092 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2600 10
Size: 54 1425 343 768 3693
Fragments arranged by size:
3693 1425 768 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2321 3046 4241 4600 4759 518
Size: 648 1002 153 725 1195 359 159 2042
Fragments arranged by size:
2042 1195 1002 725 648 359 159 153
MspA1I CmG'CkG
Cuts at: 1606 2059 3391 3519 3699 4376 5524 5769 1606
Size: 453 1332 128 180 677 1148 245 2120
Fragments arranged by size:
2120 1332 1148 677 453 245 180 128
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5731
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6283
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6283
NotI GC'GGCC_GC
Cuts at: 3730 3730
Size: 6283
NspI r_CATG'y
Cuts at: 1132 1229 3289 3641 5186 1132
Size: 97 2060 352 1545 2229
Fragments arranged by size:
2229 2060 1545 352 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6283
PciI A'CATG_T
Cuts at: 5182 5182
Size: 6283
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6283
PfoI T'CCnGG_A
Cuts at: 3115 3115
Size: 6283
PmeI GTTT'AAAC
Cuts at: 3587 3587
Size: 6283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4352 4384 5865 5897 4352
Size: 32 1481 32 4738
Fragments arranged by size:
4738 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3131 1613
Size: 1518 4765
PsiI TTA'TAA
Cuts at: 3853 3853
Size: 6283
PstI C_TGCA'G
Cuts at: 830 3411 3635 830
Size: 2581 224 3478
Fragments arranged by size:
3478 2581 224
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6283
PvuII CAG'CTG
Cuts at: 3519 3519
Size: 6283
SacI G_AGCT'C
Cuts at: 2855 2855
Size: 6283
SalI G'TCGA_C
Cuts at: 1936 3625 1936
Size: 1689 4594
SbfI CC_TGCA'GG
Cuts at: 3635 3635
Size: 6283
ScaI AGT'ACT
Cuts at: 4531 6243 4531
Size: 1712 4571
SfcI C'TryA_G
Cuts at: 826 1045 2175 2400 3407 3631 4766 5447 5638
Size: 219 1130 225 1007 224 1135 681 191
Cuts at: 5638 826
Size: 1471
Fragments arranged by size:
1471 1135 1130 1007 681 225 224 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6283
SmaI CCC'GGG
Cuts at: 1872 2691 3610 1872
Size: 819 919 4545
Fragments arranged by size:
4545 919 819
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2948 4387 5288 5550 820
Size: 197 926 1005 1439 901 262 1553
Fragments arranged by size:
1553 1439 1005 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4074 493
Size: 3581 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6283
SphI G_CATG'C
Cuts at: 1132 3289 3641 1132
Size: 2157 352 3774
Fragments arranged by size:
3774 2157 352
SspI AAT'ATT
Cuts at: 5 52 4207 5
Size: 47 4155 2081
Fragments arranged by size:
4155 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6283
StyI C'CwwG_G
Cuts at: 513 1065 2211 2251 3171 3546 3722 513
Size: 552 1146 40 920 375 176 3074
Fragments arranged by size:
3074 1146 920 552 375 176 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4310 4327 4480 4665 5110 325
Size: 1255 2730 17 153 185 445 1498
Fragments arranged by size:
2730 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4529 6241 96
Size: 274 80 33 51 1443 2552 1712 138
Fragments arranged by size:
2552 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6243 6275 6243
Size: 32 6251
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6283
XbaI T'CTAG_A
Cuts at: 3619 3619
Size: 6283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2224 3310 3553 2224
Size: 1086 243 4954
Fragments arranged by size:
4954 1086 243
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6283
XmaI C'CCGG_G
Cuts at: 1870 2689 3608 1870
Size: 819 919 4545
Fragments arranged by size:
4545 919 819
XmnI GAAnn'nnTTC
Cuts at: 4412 4412
Size: 6283
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4091 276
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI