Restriction map for
pFN21AE4153
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I FauI
FseI FspI HaeII HapII HpaI
HpaII KasI KpnI KspI MluI
MroI MspA1I MspI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflIII 671 ACGTGT A^C[GA][CT]GT
AhdI 472 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1135 GATCC GGATC(4/5)
Asp700I 1099 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 472 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1570 TGGCCA TGG^CCA
BbvCI 1293 GCTGAGG CCTCAGC(-5/-2)
BcgI 1614 GCATTTGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3 GGATAC GTATCC(6/5)
BclI 488 TGATCA T^GATCA
BsaBI 1409 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI 1114 GCAGGT ACCTGC(4/8)
BssSI 1493 CTCGTG CACGAG(-5/-1)
BstBI 1634 TTCGAA TT^CGAA
BstXI 690 CCACAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1134 AGATCC [GA]^GATC[CT]
CfoI 1057 GCGC GCG^C
DraI 1723 TTTAAA TTT^AAA
DsaI 1203 CCATGG C^C[GA][CT]GG
EaeI 1570 TGGCCA [CT]^GGCC[GA]
Eam1105I 472 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1393 GAAGAG CTCTTC(1/4)
EcoRI 313 GAATTC G^AATTC
EcoRV 571 GATATC GAT^ATC
FbaI 488 TGATCA T^GATCA
HhaI 1057 GCGC GCG^C
HinP1I 1057 GCGC G^CGC
HindIII 1684 AAGCTT A^AGCTT
Ksp632I 1393 GAAGAG CTCTTC(1/4)
MamI 1409 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1218 CAATTG C^AATTG
MflI 1134 AGATCC [GA]^GATC[CT]
MluNI 1570 TGGCCA TGG^CCA
MscI 1570 TGGCCA TGG^CCA
MunI 1218 CAATTG C^AATTG
NcoI 1203 CCATGG C^CATGG
NlaIV 463 GGCTCC GG[ACGT]^[ACGT]CC
NspV 1634 TTCGAA TT^CGAA
PstI 1112 CTGCAG CTGCA^G
SacI 172 GAGCTC GAGCT^C
SalI 480 GTCGAC G^TCGAC
SfcI 1112 CTGCAG C^T[GA][CT]AG
SfuI 1634 TTCGAA TT^CGAA
SphI 1343 GCATGC GCATG^C
XbaI 969 TCTAGA T^CTAGA
XcmI 609 CCACCTGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1134 AGATCC [GA]^GATC[CT]
XmnI 1099 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 480 GTCGAC GT^[AC][GT]AC
1148 GTCTAC
AcyI 643 GGCGTC G[GA]^CG[CT]C
729 GGCGTC
AspHI 122 GTGCTC G[AT]GC[AT]^C
172 GAGCTC
BanII 172 GAGCTC G[GA]GC[CT]^C
462 GGGCTC
BbsI 25 GAAGAC GAAGAC(2/6)
1050 GAAGAC
BpmI 175 CTCCAG CTGGAG(16/14)
1651 CTCCAG
BpuAI 25 GAAGAC GAAGAC(2/6)
1050 GAAGAC
BsaHI 643 GGCGTC G[GA]^CG[CT]C
729 GGCGTC
BsaI 433 GAGACC GGTCTC(1/5)
1648 GGTCTC
BsaJI 1203 CCATGG C^C[ACGT][ACGT]GG
1462 CCTTGG
BsiHKAI 122 GTGCTC G[AT]GC[AT]^C
172 GAGCTC
BsiYI 780 CCTCCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
851 CCTATGAGGGG
BslI 780 CCTCCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
851 CCTATGAGGGG
BsmAI 433 GAGAC GTCTC(1/5)
1649 GTCTC
BsrI 889 CCAGT ACTGG(1/-1)
1256 CCAGT
BssKI 177 CCAGG ^CC[ACGT]GG
1405 CCAGG
BstNI 177 CCAGG CC^[AT]GG
1405 CCAGG
Cfr13I 407 GGCCC G^G[ACGT]CC
1166 GGGCC
EcoRII 177 CCAGG ^CC[AT]GG
1405 CCAGG
EcoT14I 1203 CCATGG C^C[AT][AT]GG
1462 CCTTGG
HgaI 644 GCGTC GACGC(5/10)
730 GCGTC
Hin1I 643 GGCGTC G[GA]^CG[CT]C
729 GGCGTC
HincII 480 GTCGAC GT[CT]^[GA]AC
1000 GTCAAC
HindII 480 GTCGAC GT[CT]^[GA]AC
1000 GTCAAC
HphI 700 GGTGA GGTGA(8/7)
1351 GGTGA
MaeII 671 ACGT A^CGT
1085 ACGT
MseI 1627 TTAA T^TAA
1724 TTAA
MvaI 177 CCAGG CC^[AT]GG
1405 CCAGG
NspI 1233 GCATGT [GA]CATG^[CT]
1343 GCATGC
PleI 483 GACTC GAGTC(4/5)
629 GAGTC
Sau96I 407 GGCCC G^G[ACGT]CC
1166 GGGCC
ScrFI 177 CCAGG CC^[ACGT]GG
1405 CCAGG
SfaNI 289 GATGC GCATC(5/9)
292 GCATC
SmlI 169 CTTGAG C^T[CT][GA]AG
274 CTTGAG
StyI 1203 CCATGG C^C[AT][AT]GG
1462 CCTTGG
TaqI 481 TCGA T^CGA
1635 TCGA
TfiI 67 GAATC G^A[AT]TC
1596 GAATC
Tru9I 1627 TTAA T^TAA
1724 TTAA
TthHB8I 481 TCGA T^CGA
1635 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BfaI BmyI BsgI Bsp1286I
BsrDI BstF5I Cac8I DdeI DpnII
EcoT22I Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboI MboII
MnlI MslI MwoI NdeII NlaIII
NsiI RsaI Sau3AI TseI Tsp45I
Tsp509I TspRI